Mold Casting Research Articles

Numerical study on inclusion collision, coalescence and removal in an extra-thick slab continuous casting mold with M-EMS

Surface flaw detection is one of the main problems of extra-thick slab. In practice, it is considered that the application of mold electromagnetic stirring (M-EMS) is an effective means to reduce the inclusions located at the subsurface of thick slab. The present study conducted numerical studies on the inclusion transports in an extra-thick slab continuous casting mold with the effects of M-EMS. The influences of M-EMS on the inclusion collision, coalescence and removal were revealed. The predicted results indicate that the majority of inclusions entering the mold are smaller than 20 µm. The application of M-EMS promotes the collision, aggregation, and flotation of these inclusions. When the inclusions entering the mold are larger than 30 µm, the flotation removal of inclusions significantly increases, and the collision and aggregation among inclusions decreases accordingly. Furthermore, the predicted results show that inclusions larger than 200 µm in size are unlikely to follow the downward flow into the lower region of the mold, with the primary removal location concentrated near the nozzle. Large-size inclusions have been quantitatively extracted from samples with and without M-EMS using galvanostatic electrolysis method. The results indicate that the inclusions of various sizes in the slab surface layer are reduced when M-EMS is employed. Notably, inclusions sized 50–150 µm show the most significant reduction, with their mass density decreasing from 38.17 mg/10 kg to 15.11 mg/10 kg. This demonstrates a substantial reduction in large-size inclusions on the slab surface layer following the introduction of M-EMS in the mold.

Modelling smart machining process towards intelligent manufacturing - a case study

According to the concept of Industry 4.0 (even Industry 5.0) managers strive to improve production by applying information technologies and next integrating them into operational technologies, that’s enabled to transform the traditional into Intelligent Manufacturing (IM). Introduction of these changes is particularly important to improve the machining process, which is complex and dynamic. In this article the smart machining process is modelled and demonstrated for a real-world case, namely manufacturing of the die casting mould in order to increase life of the die casting mould. Therefore, firstly, the data was acquired based on the applied within a company information system and additionally on the IoT sensors from the diecasting machines, the moulds themselves and the process parameters which are used during the manufacturing process of the diecasting parts from such mould. Next, a model for predicting the life of the die-casting mould using regression analysis, based on acquired data was applied. The results of mould design and mould manufacturing process are ultimately visible on the die casting machines. Such gathered data is used to model the prediction of the possibilities for increasing life of the die casting was elaborated.

Mathematical modeling of the effect of SEN outport shape on the bubble size distribution in a wide slab caster mold

Herein, the effects of outport shapes of the submerged entry nozzle (SEN) on the size distribution of argon bubbles were studied in a wide slab caster mold by the mathematical modeling. The Eulerian–Eulerian model coupled with Multiple-Size-Group (MUSIG) approach were used to solve equations of the two phase flow and polydispersed bubbly flow in the mold, respectively. The effect of the SEN outport angle and shape on the distribution of the molten steel flow, argon gas volume fraction, and argon bubble size were investigated. The outport shapes of the SEN included large rectangular, small rectangular, square, trapezoid, and runway. Results showed that the speed of molten steel increased within the range of 260 mm from the SEN, and decreased within the range of 550 mm from the narrow face with the outport angle of SEN increased from 20° to 45°. The bubble diameter was 5.7 mm at 255 mm distance from the SEN along the mold width at different outport angles. The average speed of molten steel at the outport of SEN decreased from 0.91 to 0.72 m/s with the outport shape changed from small rectangular to runway, trapezoid, square and large rectangular. The average diameter of bubbles was approximate 5.2 mm at the outport for the five types of nozzles, and increased from 3.2 to 3.45 mm inside the mold with the outport shape changed from the runway to square, trapezoid, large rectangular and small rectangular, indicating the rate of bubble breakup was larger with the runway and square SEN casting.

The effect of cooling plate, mechanical vibration, and grain refinement on the microstructure and hardness of A380 produced by sand mold

ABSTRACT The mechanical properties of aluminium alloys can be increased by controlling the grain size and morphology of the alloy. In these studies, mechanical vibration, cooling slope plate (CSP), and grain refiner were applied on sand mould casting using A380 alloys. The hardness, and microstructure of the cast samples are investigated. These processes lead to the refinement of grain structures and a decrease in the tendency for dendritic structure formation. The Effect of the solidification time (modulus) on the microstructure is investigated. It was determined that the solidification time varies depending on the section thickness, which affects the SDAS values. It was observed that the lowest SDAS values were in CSP and the highest values were in grain refiners added casting. The lowest hardness value was recorded at CSP casting. Meanwhile, the grain refiner added vibration casting exhibits the highest hardness.

Effect of sodium content on the structure and mechanical properties of silicon bronze (Cu-3wt%Si alloys)

In the present study, the effect of sodium content on the structure and mechanical properties of Cu-3wt%Si alloys were investigated. The experimental alloys were produced with various sodium concentrations of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.5, 2 and 3% by weight using permanent mould casting technique. Tensile and hardness tests were carried out on the cast samples. Microstructures of the specimens were also analysed using optical microscopy. The results indicated that the addition of sodium to Cu-3wt%Si alloy refined and modified the structure of the alloy resulting in improvement in the ultimate tensile strength and hardness of the experimental alloy by 453.85% and 58.82% respectively at 1.5wt%Na content and percentage elongation by 168.81% at 0.1wt%Na content. The addition of sodium also led to the formation of the Na1.44Si136 intermetallic phase which further contributed to the increase in strength and hardness of the alloy.

A novel non-sinusoidal oscillator and synchronous control model for steel continuous casting mold

A novel non-sinusoidal oscillator and synchronous control model for steel continuous casting mold

A new generation of metal chillers to control the solidification structure of Al-4.5 wt%Cu alloy

This paper introduces and examines a new generation of chillers for judicious control of the solidification structure of cast metals and alloys. This innovative chiller makes use of the absorption of the latent heat of melting of a Phase Change Material (PCM) incorporated in the chiller. In this work, Al-4.5 wt%Cu melt was cast in sand molds fitted with a traditional solid steel chiller as well as this new type of chiller consisted of a steel container filled with a given amount of pure zinc as PCM. Effects of the PCM fitted chiller on the thermal history, solidification and structure of the castings were studied by experimental and computer simulation investigations. The optimum casting parameters and mold and chillers dimensions were selected using ProCast simulation software. Use of the PCM fitted chiller resulted in faster columnar-equiaxed transition, 27 %, 54 % and 40 % reduction in the length of the columnar zone and the primary and the secondary dendrite arm spacings, respectively, and about 30 % improvement in the hardness. Thermal gradients and cooling rates at different points of the PCM fitted casting were generally more than those in the traditionally chilled casting. Use of the PCM fitted chiller eliminated the formation of the feathery grains and reduced the formation of the undesired Fe-rich phases close to the chiller due to different thermal history of the castings. Segregation pattern and change of Growth Restriction Factor (GRF) along the castings were studied and related to the cooling and growth conditions experienced by each casting.

A Printable Magnetic-Responsive Iron Oxide Nanoparticle (ION)-Gelatin Methacryloyl (GelMA) Ink for Soft Bioactuator/Robot Applications.

The features or actuation behaviors of nature's creatures provide concepts for the development of biomimetic soft bioactuators/robots with stimuli-responsive capabilities, design convenience, and environmental adaptivity in various fields. Mimosa pudica is a mechanically responsive plant that can convert pressure to the motion of leaves. When the leaves receive pressure, the occurrence of asymmetric turgor in the extensor and flexor sides of the pulvinus from redistributing the water in the pulvinus causes the bending of the pulvinus. Inspired by the actuation of Mimosa pudica, designing soft bioactuators can convert external stimulations to driving forces for the actuation of constructs which has been receiving increased attention and has potential applications in many fields. 4D printing technology has emerged as a new strategy for creating versatile soft bioactuators/robots by integrating printing technologies with stimuli-responsive materials. In this study, we developed a hybrid ink by combining gelatin methacryloyl (GelMA) polymers with iron oxide nanoparticles (IONs). This hybrid ION-GelMA ink exhibits tunable rheology, controllable mechanical properties, magnetic-responsive behaviors, and printability by integrating the internal metal ion-polymeric chain interactions and photo-crosslinking chemistries. This design offers the inks a dual crosslink mechanism combining the advantages of photocrosslinking and ionic crosslinking to rapidly form the construct within 60 s of UV exposure time. In addition, the magnetic-responsive actuation of ION-GelMA constructs can be regulated by different ION concentrations (0-10%). Furthermore, we used the ION-GelMA inks to fabricate a Mimosa pudica-like soft bioactuator through a mold casting method and a direct-ink-writing (DIW) printing technology. Obviously, the pinnule leaf structure of printed constructs presents a continuous reversible shape transformation in an air phase without any liquid as a medium, which can mimic the motion characteristics of natural creatures. At the same time, compared to the model casting process, the DIW printed bioactuators show a more refined and biomimetic transformation shape that closely resembles the movement of the pinnule leaf of Mimosa pudica in response to stimulation. Overall, this study indicates the proof of concept and the potential prospect of magnetic-responsive ION-GelMA inks for the rapid prototyping of biomimetic soft bioactuators/robots with untethered non-contact magneto-actuations.

Technology for the production of sickles and knives of the Petrovka Culture of the Southern Trans-Urals (by the results of metallographic analysis)

The data of the metallographic study of sickles and knives (37 pcs) of the Petrovka Culture from the Southern Trans-Urals and the Middle Tobol River basin of the 19th–18th centuries BC are reported. The implements originate from settlements (Ustye 1, Kulevchi 3, Starokumlyak, Kamyshnoe 2, Ubagan 2, Nizhneingaly 3) and burial complexes (Ozernoe 1, Krivoye Ozero, Verkhnyaya Alabuga). The reconstruction of the manufacturing technology of the Petrovka Culture tools from the Southern Trans-Urals was carried out by both taking into account the results of the surface visual inspection, as well as by the data of the microstructural study of the metal. The metallographic analysis was conducted at the Tyumen Scientific Center of the Siberian Branch of the RAS (microscope Axio Observer D1m from Zeiss; microhardness tester PMT-3M from LOMO). A certain correla-tion was revealed between the functional purpose of the product, type of the raw material, and the tool manufacturing flowchart. The sickles and knives with handles are produced primarily from pure copper (including oxidised) both in the process of casting in mould with subsequent finishing, as well as in the result of the forming forging. The tools obtained in the casting process often had casting defects, accompanied by the phenomenon of shrinking warpage of the metal. The finishing of the copper tools was taking place in most cases either in the regime of incomplete hot forging at 300–500 C, or hot forging at 600–800 C and at near-melting temperatures of 900–1000 C. Most of the sickles in the forging process were purposefully hardened by forging on the cold metal. Unlike the sickles and knives with handles, shank knives are made mainly of low-alloyed tin bronze. Apparently, this category of tools was given a special ritual significance, especially considering the fact that about a third of the tools came from burial complexes with a specific selection of the related implements. The use of tin bronze in the production of knives sig-nificantly contributed to the fabrication of high-quality castings with the smooth surface without metal warping defects. The fini-shing of the knives after casting was carried out with heating up to 600–800 C or 900–1000 C (44 % of the tools) or in the regime of incomplete hot forging (25 %). The forging on the cold metal with annealing was rarely used. Thus, at the basis of the choice of the technological traditions of the metal production lies the availability of a certain raw material base, the type of the metal obtained from this ore, as well as the inheritance of the technologies from the preceding cultural communities. Technological inno-vations in the processing of non-ferrous metal, associated with the supply of Sn-bronzes in the form of ingots or finished products from Central Kazakhstan to the Southern Trans-Urals, led to the significant increase in the quality of the produce.

Numerical Simulation of Flow and Argon Bubble Distribution in a Continuous Casting Slab Mold under Different Argon Injection Modes

A three-dimensional model is established to investigate the effect of argon injection mode, argon flow rate and casting speed on the gas–liquid two-phase flow behavior inside a slab continuous casting mold. The Eulerian–Eulerian model is employed to simulate the gas–liquid flow, and the population balance model is applied to describe the bubble breakage and coalescence process in the mold. The numerical simulation results of the bubble size distribution are verified using the water model experiment. The results show that the flow field and bubble distribution are similar between the argon injection at the upper submerged entry nozzle (SEN) and tundish upper nozzle (TUN), while the number density is larger for the argon injection of TUN. The coalescence rate of bubbles and the bubble size inside the mold increase with increasing argon flow rate. When the argon flow rate exceeds 4 L/min, the flow pattern of liquid steel changes from double-roll flow to complex flow, with aggravation of the level fluctuation of the top surface near the SEN. When the casting speed increases, the bubble breakup rate increases and results in a decrease in the size of bubbles inside the mold. At a high casting speed, the flow pattern tends to form double-roll flow, and the liquid level at the narrow face of the top surface increases.

Physical modeling and numerical investigation of fluid flow and solidification behavior in a slab caster mold using hexa‐furcated nozzle

AbstractSlag entrapment from metal–slag interface during continuous casting operations has been a major area of concern for steelmakers globally. The presence of inactive regions in the upper region of the mold poses another challenge. Proper flow behavior of the molten metal coming out of the nozzle in the mold is required to overcome these challenges. Nozzle design greatly affects the flow pattern of the molten steel inside the mold. The present investigation is an attempt to study the flow and solidification behavior in a slab caster mold with the use of a novel‐designed hexa‐furcated nozzle using numerical investigation results. The casting speed and submerged entry nozzle (SEN) depth are varied to study the effect of these parameters on minimizing the inactive zones in the mold and the steel/slag interface fluctuations. The results show that the interface fluctuation increases at higher casting speed and lower SEN depth. The residence time distribution (RTD) analysis was also performed for different cases to investigate the flow behavior. The validation of the fluid flow and RTD curve inside the computational domain is carried out with the use of physical modeling.

Mass Production and Vast Application States of Bulk Metallic Glasses

Since the first synthesis of bulk metallic glasses (BMGs) by copper mold casting in 1990, much effort has been devoted to the searching of new BMG composition, the clarification of fundamental and engineering properties for BMGs and their industrialization. At present, BMGs have been formed in a large number of multicomponent alloy systems where the empirical three component rule is satisfied. Nowadays, commercialized BMGs are classified to Zr-based and Fe-based alloy groups. When we look at the industrialization of Zr-Al-Ni-Cu-based BMGs, the first commercialization was made for golf clubs in Japan in 1998, followed by watch parts etc. Since then, Zr-based BMGs have been used continuously up to 2013, though their application scale was in a limited state. Since 2014, the application scale was significantly extended in collaboration with the rapid developments of smartphones and electric vehicles. At present, the mass production facilities for Zr-based BMGs have been significantly developed and variety of BMG products have been produced. On the other hand, Fe-based soft magnetic BMGs were found in 1995. Their BMGs have also been used on a huge number of pieces in various kinds of electronic-magnetic instruments. These recent application states for Zr- and Fe-based BMGs are introduced together with new nanocrystalline Fe-based soft magnetic alloys developed through the derivation of alloy composition from Fe-based BMGs.

Can Multifunctionality of Bioresorbable BMGs be Tuned by Controlling Crystallinity?

Ca-Mg-Zn bulk metallic glasses (BMGs) are promising biomaterials for orthopaedic applications because when they get reabsorbed, a retrieval surgery is not needed. In this study, Ca-Mg-Zn metallic glasses with different compositions, Ca56.02Mg20.26Zn23.72 and Zn50.72Mg23.44Ca25.84, were fabricated by induction melting followed by copper mould casting. Their degree of crystallinity was modified by annealing, obtaining exemplar specimens of fully amorphous, partially amorphous (i.e., a BMG composite (BMGC)) and fully crystalline alloys. The microstructure, thermodynamic and corrosion performance of these alloys were evaluated as well as their electrochemical behaviour. The results of polarisation tests demonstrate that the corrosion resistance of the Zn-rich alloy is markedly better than the Ca-rich BMG. Corrosion rates of these Ca-and Zn-rich alloys with different degrees of crystallinity illustrate that the corrosion behaviours of alloys strongly depend on their microstructure, which shows a positive correlation between the corrosion current density and the crystallised volume fraction of the alloy. This study aims to shed light on the impact of the amorphicity-to-crystallinity ratio on the multifunctional properties of BMGs/BMGCs, and to assess how feasible it is to fine-tune those properties by controlling the percentage of crystallinity.

Ultra‐high performance alkali‐activated slag as a reusable mold for light metal casting

AbstractLight metal die casting is usually performed using steel molds. However, these lead to a reduced quality of the casting due to the occurrence of metal corrosion on the surface and the incorporation of hydrogen into the casting as a result of required process chemistry. Ultra‐high performance concrete based on alkali‐activated slag can be used to produce mineral molds for aluminum casting. The use of reusable mineral molds not only enables the production of various thin‐walled geometries. The risk of metal corrosion is eliminated and the concrete molds can withstand multiple cycles due to their thermal stability and high strength, making them potentially superior to the already common lost mineral molds.

Deep learning–based inline monitoring approach of mold coating thickness for Al-Si alloy permanent mold casting

AbstractIn the permanent mold casting process, the distribution of mold coating thickness is a significant variable with respect to the coating’s thermal resistance, as it strongly influences the mechanical properties of cast parts and the thermal erosion of expensive molds. However, efficient online coating thickness measurement is challenging due to the high working temperatures of the molds. To address this, we propose an indirect monitoring concept based on the analysis of the as-cast surface corresponding to the coated area. Our previous research proves linear correlations between the as-cast surface roughness parameter known as arithmetical mean height (Sa) and the coating thickness for various coating materials. Based on these correlations, we can derive the coating thickness from the analysis of the corresponding as-cast surface. In this work, we introduce a method to quickly evaluate the as-cast surface roughness by analyzing optical images with a deep-learning model. We tested six different models due to their high accuracies on ImageNet: Vision Transformer (ViT), Multi-Axis Vision Transformer (MaxViT), EfficientNetV2-S/M, MobileNetV3, Densely Connected Convolutional Networks (DenseNet), and Wide Residual Networks (Wide ResNet). The results show that the Wide ResNet50-2 model achieves the lowest mean absolute error (MAE) value of 1.060 µm and the highest R-squared (R2) value of 0.918, and EfficientNetV2-M reaches the highest prediction accuracy of 98.39% on the test set. The absolute error of the surface roughness prediction remains well within an acceptable tolerance of ca. 2 µm for the top three models. The findings presented in this paper hold significant importance for the development of an affordable and efficient online method to evaluate mold coating thickness. In future work, we plan to enrich the sample dataset to further enhance the stability of prediction accuracy.

Precipitation Kinetics of Water-Cooled Copper Mold Al-Mg-Si(-Mn, Zr) Alloy during Aging

The aging precipitation behavior of 6061 aluminum alloy that underwent iron casting and water-cooled copper casting and 6061 aluminum with Mn and Zr elements added was studied. Firstly, the hardness curves, tensile properties, and fracture morphology of four aging alloys—6061 (iron mold casting), 6061 (water-cooled copper mold casting), 6061-0.15Mn-0.05Zr (iron mold casting), and 6061-0.15Mn-0.05Zr (water-cooled copper mold casting)—were studied. The results of the aging hardness curve show that the aging precipitated phase of the 6061 alloy cast with a water-cooled copper mold is dispersed. The addition of Mn increases the amount of coarse inclusion α-(AlMnFeSi) in the alloy, resulting in a decrease in the age hardening property. The addition of Zr is related to the nucleation and growth of the G.P. region in the early aging period, mainly changing the formation rate and quantity of the G.P. region, leading to the advancement of peak aging and an increase in hardness. After the G.P. region gradually transforms into the β phase, the hardness of the alloy increases with the increase in the volume fraction of the β phase. When the β″ phase is coarsened to the point where the fault line can be bypassed, the transitional metastable β′ phase begins to precipitate, and the coherent distortion around it weakens, indicating over-aging. Finally, the equilibrium phase Mg2Si is formed. The results of the tensile tests indicate that the tensile strength and yield strength of the 6061-0.15Mn-0.05Zr alloy produced by water-cooled copper casting after aging are 356 Mpa and 230 Mpa, respectively. These values are 80 MPa and 75 MPa higher, respectively, than those of the 6061 aluminum alloy produced via iron casting. However, the elongation is by 5%. The fracture morphology of the tensile sample of the aging alloy shows that dislocation slip in the alloy results in dislocation plugging, stress concentration, and the initiation of crack cleavage on the surface. The fracture of the water-cooled copper mold-casting alloy is a ductile fracture of the microporous aggregation type, and the macroscopic fracture exhibits an obvious “neck shrinkage” phenomenon. The fracture analysis is consistent with the mechanical properties. The DSC curve shows that there is no enrichment process of solute atoms during the heating process, and the aging precipitation process after homogenization is as follows: G.P. zone → β″ phase → β′ phase. The aging precipitation process of the water-cooled copper casting alloy after homogenization treatment is as follows: β″ phase → β′ phase (no precipitation in the G.P. zone was observed). The results of the differential scanning calorimetry (DSC) analysis show that the main strengthening phase in the experimental alloy system is the β″ phase. The activation energies for the β″ phase precipitation were calculated and found to be 147 KJ/mol, 217 KJ/mol, 185 KJ/mol, and 235 KJ/mol, respectively. Additionally, a kinetic equation for the β″ phase precipitation during alloy aging was fitted.

Numerical Analysis of Slag–Steel–Air Four-Phase Flow in Steel Continuous Casting Model Using CFD-DBM-VOF Model

Argon injection is usually applied in the continuous casting mold to prevent submerged entry nozzle (SEN) clogging. However, the stability of the slag–steel interface is affected by the injected gas, even leading to the formation of the slag eye. A computational fluid dynamics–discrete bubble model–volume of fluid (CFD-DBM-VOF) model is established to predict the argon–slag–steel–air four-phase flow in the continuous casting mold. The bubble behavior is treated with the Lagrangian approach considering bubble coalescence and breakup. The movement behavior of the slag–steel interface is analyzed with and without argon blowing, validated with the water model. The results show that the large bubble tends to float up into the slag–steel interface near the SEN with argon injection, resulting in fluctuations in the slag–steel interface near the SEN. The bubble distribution, flow field, fluctuation height of the slag–steel interface and configuration of the slag eye in the mold are analyzed. Furthermore, the effect on the casting speed, gas flow rate and thickness of the slag layer is obtained based on the result. The mathematical prediction results showcase a combination of well-established phenomena and newly generated predictions.

Effect of Sn Addition on the Microstructure and Age-Hardening Response of a Zn-4Cu Alloy

The aim of this research is to assess the influence of Sn inclusion on the microstructure evolution and age-hardening response of a Zn-4Cu alloy. This is the first study to correlate the age-hardening response to the microstructure of Zn-4Cu alloy reinforced with different Sn contents. A series of Zn-4Cu-Sn alloys were successfully fabricated with different Sn concentrations in the range of 0.0–4.0 wt.% using permanent mold casting. The microstructure of Zn-4Cu-Sn alloys was investigated by means of a scanning electron microscope (SEM) attached with an energy dispersive spectroscope (EDS) and X-ray diffraction (XRD) line profile analysis. At room temperature, the Vickers microhardness measurements were used to assess the age-hardening response of alloys. The results show that the microhardness of the Zn-4Cu (ZC) binary alloy increases a little bit from 76 to 80 HV as the aging time increases from 2 to 128 h, respectively. For aging times up to 16 h, the microhardness of all Sn-containing alloys decreases but then increases again. The lowest hardness belongs to the ZC-1.5Sn alloy, and the Sn-Zn-3.0Sn alloy has the highest; the other alloys fall somewhere in between. At high aging times (64 and 128 h), the microhardness of all Sn-containing samples increased continuously with an increasing Sn content from 0.0 to 3.0 wt.%. When the Sn-containing alloys (3.5 and 4.0 wt.% Sn) were aged for 64 and 128 h, the hardness declined by 7.94% and 8.90% compared to their peak aging hardness values, respectively. By considering the structural changes that occur in the Zn-4Cu-Sn alloys, the reasons for the observed variations in microhardness data with increasing Sn content and aging time were elucidated. X-ray diffraction (XRD) data was analyzed to determine the zinc matrix’s lattice parameters, c/a ratio, and unit cell volume variations.

Abstract 18445: Rigorous Mechanics in Engineered Heart Tissues

Introduction: Heart failure can develop or be exacerbated by mechanical stressors, including increased diastolic strain (preload) and increased systolic impedance (afterload). Recent advances in human induced pluripotent stem cell (iPSC) technology and tissue engineering have enabled long term culture and maturation of engineered heart tissues (EHTs). Most current platforms do not allow for mechanical assessment under a range of loading conditions. Here, we develop a technique to engineer cardiac tissues that allow for non-destructive rigorous phenotyping of tissue mechanics. Methods: Custom tissue anchors were 3D printed using Boston Micro Fabrication HTL Resin and placed within polydimethylsiloxane tissue casting molds. iPS cardiomyocytes and ventricular fibroblasts were mixed 5:1 in 2 mg/mL collagen and cast in rectangular molds. Tissues were compacted around custom tissue anchors over 14 days. Tissues were dismounted from the molds and fixed for immunostaining or hooked onto a force transducer for length-tension, force-frequency, and work-loop analysis. Results: 3D printed tissue anchors accommodated EHT molding and compaction. EHTs spontaneously contracted after 3 days in culture, and compacted to 15.9±0.8% their initial tissue area by 14 days of culture. Engineered tissues were cell dense and mature, with bands of aligned sarcomeres. During isometric testing, tissues display a physiologic length-tension relationship, with linear increases in force with stretch between L 0 (slack length) and L max (length at maximum active force generation, 4.3±0.7 mN/mm 2 ; r 2 =0.96). These are coupled with increases in contraction and relaxation velocities. EHTs were capable of mimicking physiologic work loops. Conclusions: In summary, we have shown the ability to grow and mature three-dimensional human engineered heart tissues capable of rigorous mechanical assessment, enabling future studies on load-dependent cardiac remodeling.

Effect of flow characteristics in the inner gate on the microstructure of a semi-solid processed hypereutectic Al–Si alloy

ABSTRACTThis study aims to prepare hypereutectic Al–Si alloy cylindrical samples with hard inner surfaces by changing the depth of inner gates in squeeze casting molds. The characteristic parameters (volume fraction and equivalent diameter) of the hard phases (primary Si and Fe-rich phases) and the hardness of the samples were observed. When the depth of the inner gate is 5 mm, there is a radial gradient in the characteristic parameters of the hard phases in the members resulting in surface strengthening of the structural member. However, when the depth of the inner gate is 9 mm, there is no radial gradient. The mechanisms of achieving surface strengthening in hypereutectic Al–Si alloy cylindrical samples by changing the rheological characteristics of semi-solid Al–Si alloy slurries were revealed.