Heat treatment is one of the essential operations widely used in most dairy processes, and heat stability is one of the essential properties of milk. Casein micelles are the major component in milk responsible for the heat stability of milk during processing. This study assessed the effects of heat treatment temperature and duration on the average size, turbidity, polydispersity index and heat stability of casein micelles in yak skim milk and distilled water. The results showed that whey protein had an important role in influencing the heat stability of casein micelles. The average size, polydispersity index and turbidity of micelles in skim milk were higher than those of micelles in distilled water in all cases while the heat stability of casein micelles in skim milk was lower than those in distilled water. As a result of the heat treatment, the size of micelles in skim milk increased due to complex of casein/whey protein formed via covalent bonds, whereas it decreased in distilled water attributed to the change of hydrophobicity in micelles. The size distribution of particles broadened with increasing heating temperature, resulting in the increase in turbidity and polydispersity index of casein micelles both in skim milk and distilled water. The micelles in skim milk combined with whey protein during heating. These findings will help processors design appropriate heating conditions for yak milk and yak casein products and help identify new opportunities for product development.