Cartilage Lesions Research Articles

Tongbi Huoluo Decoction alleviates cartilage degeneration in knee osteoarthritis by inhibiting degradation of extracellular matrix

BackgroundKnee osteoarthritis (KOA) is an age-related degenerative disease characterized by abrasion of articular cartilage. Tongbi Huoluo Decoction (TBHLD) has been transformed from the famous traditional Chinese medicine Duhuo Jisheng Decoction, which can effectively alleviate pain symptoms in KOA. However, the active components and mechanisms of TBHLD in treating KOA have not yet been elucidated. The purpose of the study was to demonstrate the molecular mechanism of TBHLD in treating KOA.MethodsThe components and targets of TBHLD and KOA were collected from multiple databases, and the protein to protein interaction (PPI) network was constructed. Next, we performed topological calculation and enrichment analysis. Besides, we performed virtual screening for molecular docking and molecular dynamics simulation (MDS). Furthermore, the vitro and vivo experiments were performed to evaluate the validity and mechanism of TBHLD.Results206 active components and 187 potential targets were screened from Tongbi Huoluo Decoction. A total of 50 intersecting genes were identified between TBHLD and KOA, 20 core targets were calculated by network topology analysis. The core targets were enriched in the ECM interaction pathways. The results of virtual screening for molecular docking and MDS showed that the active components of TBHLD had steady binding conformations with core genes. Moreover, we identified 32 differential serum components in TBHLD-containing serum using LC–MS, including 22 upregulated and 10 downregulated serum components. TBHLD improved the proliferation activity of OA chondrocytes, decreased the expression of Col1a1, Col1a2, Mmp2, Mmp13 in OA chondrocytes, ameliorated the cartilage lesions and restored the cartilage abrasion.ConclusionTBHLD inhibited degradation of cartilage ECM by regulating the expression of type I collagens and Mmps to ameliorate cartilage degeneration in KOA.

Predilection of Patellofemoral Cartilage Lesions in Patients with Posterior Medial Meniscal Root Lesions.

The present study aims to compare the presence and severity of patellofemoral osteoarthritis between patients with root lesions and non-root lesions. A total of 102 patients were included in this study (51 root lesions and 51 non-root lesions). The root lesion cohort was matched to a non-root lesion cohort based on sex, body mass index, and age at the time of surgery. Radiographic evaluation with modified Outerbridge scoring of MRI of the knee was performed to determine the severity of degeneration of the knee joint preoperatively. Mann-Whitney and Independent t tests were used to compare the groups. The root lesion group had statistically greater Outerbridge patella scores (M = 2.45 ± 1.12) and trochlear scores (M = 2.27 ± 1.37) than the non-root lesion patients (M = 1.78 ± 1.30, P = 0.006, and M = 1.55 ± 1.40, P = 0.010, respectively). When using a new scale for grading patellofemoral arthritis, the root lesion group had statistically greater scores (M = 8.33 ± 3.38) than the non-root lesion patients (M = 5.67 ± 3.07) (P < 0.001). Patients with root lesions have a greater degree of patellofemoral cartilage lesions than patients without root lesions. The presence of cartilage lesions preoperatively in root lesion patients has presented the question of whether repair is worthwhile or if one should delay surgery until arthroplasty is indicated. Future research should be carried out on outcomes of root repair surgery in patients with patellofemoral cartilage lesions, in addition to considering the patient's age, activity level, and other risk factors.

Reliability of Conventional Hip MRI in Detecting Labral Tear and Labrocartilagenous Lesions in cases of Femoroacetabular Impingement, A Comparative Study with Hip Arthroscopy.

Imaging studies play a crucial role in diagnosing femoroacetabular impingement (FAI), including plain radiography and Magnetic Resonance Imaging (MRI). FAI is a combined pathology of bony abnormality, labral and labrocartilagenous erosions. Surgical treatment for such cases has become more established and preoperative imaging is the roadmap that includes the assessment of labrum and articular cartilage. During a period of 2 years, thirty-seven patients with a clinical diagnosis of FAI were retrospectively enrolled in this study, including 17 men and 20 women, aged 27-62 years. There were 22 right hips and 15 left hips. MRI was done for all patients to identify bony details, labral and chondral abnormalities and to exclude coexisting disorders. The imaging findings were compared with the arthroscopic data. 15 patients had Pincer FAI, 11 patients had CAM, and 11 patients had combined Cam/Pincer FAI. Labral tear was detected in 100 % of patients, 97 % had an anterosuperior labral tear. 82 % of patients had partial thickness cartilage lesions and 8 % had full thickness cartilage lesions. MRI had a sensitivity of 100 % compared to hip arthroscopy in detecting labral tear, 60 % in detecting cartilage erosion. Conventional hip MRI detects bony changes in FAI, type of impingement and associated labral tear and cartilage erosions in comparison to the hip arthroscopy.

Deep Learning Convolutional Neural Network Reconstruction and Radial k-Space Acquisition MR Technique for Enhanced Detection of Retropatellar Cartilage Lesions of the Knee Joint.

To assess diagnostic performance of standard radial k-space (PROPELLER) MRI sequences and compare with accelerated acquisitions combined with a deep learning-based convolutional neural network (DL-CNN) reconstruction for evaluation of the knee joint. Thirty-five patients undergoing MR imaging of the knee at 1.5 T were prospectively included. Two readers evaluated image quality and diagnostic confidence of standard and DL-CNN accelerated PROPELLER MR sequences using a four-point Likert scale. Pathological findings of bone, cartilage, cruciate and collateral ligaments, menisci, and joint space were analyzed. Inter-reader agreement (IRA) for image quality and diagnostic confidence was assessed using intraclass coefficients (ICC). Cohen's Kappa method was used for evaluation of IRA and consensus between sequences in assessing different structures. In addition, image quality was quantitatively evaluated by signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) measurements. Mean acquisition time of standard vs. DL-CNN sequences was 10 min 3 s vs. 4 min 45 s. DL-CNN sequences showed significantly superior image quality and diagnostic confidence compared to standard MR sequences. There was moderate and good IRA for assessment of image quality in standard and DL-CNN sequences with ICC of 0.524 and 0.830, respectively. Pathological findings of the knee joint could be equally well detected in both sequences (κ-value of 0.8). Retropatellar cartilage could be significantly better assessed on DL-CNN sequences. SNR and CNR was significantly higher for DL-CNN sequences (both p < 0.05). In MR imaging of the knee, DL-CNN sequences showed significantly higher image quality and diagnostic confidence compared to standard PROPELLER sequences, while reducing acquisition time substantially. Both sequences perform comparably in the detection of knee-joint pathologies, while DL-CNN sequences are superior for evaluation of retropatellar cartilage lesions.

Diagnostic Performance of Multi-Detector Computed Tomography Arthrography and 3-Tesla Magnetic Resonance Imaging to Diagnose Experimentally Created Articular Cartilage Lesions in Equine Cadaver Stifles.

The purpose of the study was to determine the diagnostic performance of computed tomographic arthrography (CTA) and 3-Tesla magnetic resonance imaging (MRI) for detecting artificial cartilage lesions in equine femorotibial and femoropatellar joints. A total of 79 cartilage defects were created arthroscopically in 15 cadaver stifles from adult horses in eight different locations. In addition, 68 sites served as negative controls. MRI and CTA (80-160 mL iodinated contrast media at 87.5 mg/mL per joint) studies were obtained and evaluated by a radiologist unaware of the lesion distribution. The stifles were macroscopically evaluated, and lesion surface area, depth, and volume were determined. The sensitivity and specificity of MRI and CTA were calculated and compared between modalities. The sensitivity values of CTA (53%) and MRI (66%) were not significantly different (p = 0.09). However, the specificity of CTA (66%) was significantly greater compared to MRI (52%) (p = 0.04). The mean lesion surface area was 11 mm2 (range: 2-54 mm2). Greater lesion surface area resulted in greater odds of lesion detection with CTA but not with MRI. CTA achieved a similar diagnostic performance compared to high-field MRI in detecting small experimental cartilage lesions. Despite this, CTA showed a higher specificity than MRI, thus making CTA more accurate in diagnosing normal cartilage. Small lesion size was a discriminating factor for lesion detection. In a clinical setting, CTA may be preferred over MRI due to higher availability and easier image acquisition.

Surgical Treatment ofFocal Chondral Lesions ofthe Knee inthe Military Population: Current andFuture Therapies.

Chondral and osteochondral defects of the knee are common injuries in the military population that have a significant impact on readiness. Definitive treatment of these injuries is challenging since cartilage has a limited capacity for self-repair and regeneration. Management is particularly challenging in military patients who maintain a higher level of activity similar to athletes. Existing surgical techniques have variable results and often long recovery times, sparking the development of several new innovative technologies to return service members back to duty more quickly and effectively after cartilage injury. The purpose of this article is to review the current and future surgical treatments for chondral and osteochondral knee lesions and their relevance in managing these injuries in the military. In this review article, we describe the current treatments for chondral and osteochondral defects of the knee, reporting on outcomes in military populations. We explore emerging treatment modalities for cartilage defects, reporting innovations, stage of research, and current data. Published results of each treatment option in military populations are reviewed throughout the article. This review includes 12 treatments for chondral lesions. Of these therapies, four are considered synthetic and the remaining are considered regenerative solutions. Regenerative therapies tend to perform better in younger, healthier populations with robust healing capacity. Success of treatment depends on lesions and patient characteristics. Nearly all modalities currently available in the USA were successful in improving patients from presurgical function in the short (<6 months) term, but the long-term efficacy is still challenged. Upcoming technologies show promising results in clinical and animal studies that may provide alternative options desirable for the military population. The current treatment options for cartilage lesions are not entirely satisfactory, usually with long recovery times and mixed results. An ideal therapy would be a single procedure that possesses the ability to enable a quick return to activity and duty, alleviate pain, provide long-term durability, and disrupt the progression of osteoarthritis. Evolving technologies for cartilage lesions are expanding beyond currently available techniques that may revolutionize the future of cartilage repair.

Hip rotation muscle strength is implicated in the progression of early post-traumatic osteoarthritis: A longitudinal evaluation up to 5 years following ACL reconstruction

IntroductionFollowing ACL reconstruction (ACLR), deficits in hip muscle strength and relationships to future outcomes are unknown. Methods111 participants one year after ACLR, completed hip external rotation (ER) and internal rotation (IR) strength assessment. At 1 (n = 111) and 5 (n = 74) years post-ACLR, participants completed a battery of functional, symptomatic (Knee Osteoarthritis Outcome Score (KOOS)) and structural assessments (radiography, magnetic resonance imaging (MRI)). Cartilage health of the patellofemoral and tibiofemoral compartments was assessed with the semiquantitative MRI Osteoarthritis Knee Score. Hip rotation strength was compared between-limbs, and relationships between hip strength at 1 year and functional, symptomatic and cartilage outcomes at 1 and 5 years were investigated with regression models. ResultsThe index (ACLR) limb had weaker hip ER (but not IR) strength compared to the contralateral side (standardised mean difference ER = −0.33 (95%CI -0.60, −0.07; IR = −0.11 (95%CI -0.37, 0.15). Greater hip ER and IR strength was associated with superior function at 1 and 5 years, and better KOOS-Patellofemoral symptoms at 5 years. Greater hip ER strength was associated with lower odds of worsening tibiofemoral cartilage lesions at 5 years (odds ratio 0.01, 95%CI 0.00, 0.41). ConclusionHip rotation strength may play a role in worsening function, symptoms and cartilage health after ACLR.

Additional Anterolateral Ligament Reconstruction Helps Patients Improve Dynamic Postural Stability in Revision Anterior Cruciate Ligament Reconstruction.

Background and Objectives: The goal in treating anterior cruciate ligament (ACL) injury especially in revision cases is return to sports activity by regaining dynamic postural stability. Among various methods to achieve this goal, additional anterolateral ligament reconstruction (ALLR) is gaining attention. The purpose of this study was to evaluate the effects of additional ALLR in revision ACL reconstruction (RACLR). Materials and Methods: Patients who underwent RACLR between July 2015 and June 2018 were enrolled. The exclusion criteria were less than 1-year follow-up, age older than 45 years, concomitant multiple ligament injuries, contralateral knee injury, subtotal or total meniscectomized state, and articular cartilage lesions worse than Outerbridge grade 3. Thirty-nine patients (20 patients; RACLR only (Group A), 19 patients; RACLR with additional ALLR (Group B)) were included. Clinical scores (Lysholm score, subjective International Knee Documentation Committee (IKDC) score, Tegner activity scale), isokinetic strength test, single-leg-hop for distance test (SLHDT), Y-balance test (YBT) were checked preoperatively and 1-year postoperatively. Results: Limb symmetry index values in YBT showed significantly better result in Group B 1-year postoperatively (Group A: 97.2 ± 4.0, Group B: 100.3 ± 2.9, p = 0.010), although there were no differences preoperatively between groups (Group A: 90.4 ± 6.7, Group B: 89.3 ± 5.5, p = 0.594). Regarding clinical scores, isokinetic strength tests, and SLHDT, there were no differences between groups preoperatively nor 1-year postoperatively. Conclusions: Additional ALLR in RACLR helped patients gain better dynamic postural stability at 1-year postoperative follow-up.

Poster 290: Effect of Platelet-rich Plasma Injections on Cartilage T1rho in Knee Osteoarthritis

Poster 290: Effect of Platelet-rich Plasma Injections on Cartilage T1rho in Knee Osteoarthritis

Poster 294: Patellar Tendon Insertion is Not Distalized following Tibial Tubercle Osteotomy with Distalization

Objectives: Patella alta is a common anatomic risk factor in patients with patellofemoral instability and/or patellofemoral cartilage lesions, occurring in nearly 25% of these patients. Distalization tibial tubercle osteotomy (TTO) is effective in patients with patella alta in association with patellofemoral instability and cartilage lesions. Prior studies have suggested that increased patellar tendon length itself is a risk factor for recurrent patellofemoral instability, and have proposed the addition of a patellar tendon tenodesis via placement of a suture anchor proximal to the distalization TTO. However, concerns regarding increased patellofemoral cartilage stresses with addition of patellar tendon tenodesis have limited its adoption. The purpose of this study was to evaluate patellar tendon length and alignment parameters on preoperative and postoperative MRI and to evaluate patient reported outcomes (PROs) following distalization tibial tubercle osteotomy (TTO) without patellar tendon tenodesis in patients with patella alta. Methods: We conducted a retrospective review of 20 adult patients who underwent distalization TTO for patellar alta at our institution (December 2014-August 2021). All patients underwent preoperative and postoperative magnetic resonance (MR) imaging of the affected knee. Distalization TTO utilized a wedge-shaped osteotomy measuring ˜5cm in length and was fixed with two 4.5mm bicortical fully threaded screws following TT transfer. Caton-Deschamps (CDI), axial and sagittal TT-TG indices1, patellar tendon length and distances from tibial plateau to patellar tendon insertion and tibial tubercle were assessed (Figure 1). PROs included IKDC Subjective Knee Evaluation, KOOS Quality of Life Form, Kujala Anterior Knee Pain From, and Veterans RAND 12 Item Mental (VR12-MH) and Physical Health (VR12-PH) Surveys. Clinical data, including demographics, complication and perioperative data, was obtained from preoperative and postoperative charts in our electronic medical record. Results: Average age at time of surgery was 27.4 years (range: 14 – 42 years) and all patients were female. Average BMI was 24.9 +/- 4.4 kg/m2. Eleven patients (55%) were diagnosed with isolated patellofemoral cartilage lesions, four patients (20%) were diagnosed with patellofemoral instability without associated cartilage lesions and five patients (25%) were diagnosed with both instability and cartilage lesions. At the time of TTO distalization, eleven patients (55%) underwent matrix-induced autologous chondrocyte implantation (MACI), nine patients (45%) underwent MPFL reconstruction, four patients (20%) underwent lateral release/lengthening, and three patients (15%) underwent osteochondral allograft (OCA) implantation. Fourteen patients (70%) underwent anteromedialization of the tibial tubercle in addition to distalization. Radiographic parameters demonstrated improved patellar height (CDI decreased from 1.36 to 1.11 (p &lt; 0.001), improved rotational alignment (axial TT-TG decreased from 14.3mm to 11.1mm (p = 0.018)) and anteriorization of the TT (sagittal TT-TG increased from -4.3mm to -1.4mm (p = 0.037)) following distalization TTO. Distance from the tibial plateau to the PT insertion decreased from 20.1mm preoperatively to 17.9mm postoperatively (p&lt; 0.020) and patellar tendon length decreased from 53.4mm preoperatively to 50.0mm postoperative (p &lt; 0.001). Average distalization was 6.4mm. IKDC scores increased from 37.1 to 60.5 (p = 0.011), KOOS QOL scores increased from 19.5 to 42.1 (p &lt; 0.001), Kujala scores increased from 50.9 to 70.6 (p = 0.018), VR12-MH scores increased from 52.7 to 59.2 (p = 0.024) and VR12-PH scores increased from 35.1 to 41.7 (p = 0.009). Of the patients with preoperative patellofemoral instability, there were no cases of recurrent instability postoperatively. Ten patients (50%) underwent elective removal of hardware of TTO screws at an average of 10.7 months. There were four (20%) complications in this cohort, with two cases (10%) of postoperative arthrofibrosis requiring manipulation under anesthesia, one case (5%) of postoperative MRSA infection of the MPFL graft and one case (5%) of tibial tubercle delayed union requiring bone grafting and revision fixation. Conclusions: Distalization TTO without patellar tendon tenodesis is an effective surgical technique for improving patellar height in patients with patella alta and patellofemoral instability and/or patellofemoral cartilage lesions, with improvements in radiographic parameters and PROs postoperatively. There were no cases of recurrent instability in this cohort. The patellar tendon insertion does not appear to be significantly distalized following distalization TTO, likely related to scarring of the patellar tendon just proximal to the osteotomy site postoperatively. Given the complexity of risk factors associated with patellofemoral instability and patellofemoral cartilage lesions, distalization TTO provides an additional tool for patient-specific surgical management, without the need for patellar tendon tenodesis.

Poster 252: BMAC Augmentation of Allograft Anterior Cruciate Ligament Reconstruction Improves Patient Reported Outcomes in the Presence of Intra-articular Pathology

Objectives: A randomized control trial demonstrated evidence of earlier graft remodeling and improved 9-month International Knee Documentation Committee (IKDC) scores when anterior cruciate ligament reconstruction (ACLR) with bone-tendon-bone allograft was augmented with an intra-graft injection of bone marrow aspirate concentrate (BMAC). While these results are promising, the mechanism by which BMAC may affect patient reported outcome measures (PROMs) following ACLR is unclear. Methods: This was a sub-analysis of patients enrolled to date in an IRB-approved, double-blinded, randomized control trial comparing patients undergoing ACLR with BTB allograft ± BMAC. Only patients who had completed at least 9 months follow-up were included. Patients were stratified by 1) treatment arm (BMAC versus control) and 2) presence of concurrent meniscus or cartilage pathology, as noted on diagnostic arthroscopy). The primary outcome was the Tegner activity scale and International Knee Documentation Committee (IKDC) at 9 months. A secondary sub-analysis assessed differences in MRI characteristics (signal intensity ratio [SIR], graft volume) by group. Results: The final study cohort included 44 BMAC patients – 19 (43%) without and 25 (57%) with concurrent cartilage or meniscus pathology – and 39 control group patients - 16 (41%) without and 23 (59%) with concurrent cartilage or meniscus pathology. 24 patients in the BMAC group had a meniscus lesion, 13 of which were treated via meniscectomy (54%) and 11 by meniscus repair (46%). By comparison, 21 patients in the control group had a meniscus lesion (P=0.57), 11 who were treated with meniscectomy (52%, P=0.88) and 11 who had a meniscus repair (P=1.00). 5 patients with BMAC (20%) and 6 control patients (25%) had a cartilage injury noted intraoperatively (P=0.94); chondroplasty was performed in 2 patients in the BMAC group (8.0%) and 4 patients in the control group (16.7%). Tegner score at 9 months did not differ by BMAC treatment or presence of concurrent meniscus/cartilage pathology (P=0.816). If a patient had a concurrent meniscus and/or cartilage lesion, the group treated with BMAC had a greater mean IKDC at 9 months (81.7 [SD=10.2] vs. 74.3 [SD=14.6], P=0.039). If a patient had no concurrent pathology, there was no difference in IKDC scores by treatment group (P=0.80). Change in Tegner (P=0.99) and IKDC (P=0.31) did not differ by groups. At 3 months, patients with BMAC and concurrent pathology had the highest mean SIR in the inferior 1/3 of the graft (3.00), followed by patients with BMAC but no concurrent pathology (2.60), controls without concurrent pathology (1.95), and controls with concurrent pathology (1.83, P=0.041). There were no significant differences in SIR at 9 months or graft volume, in this comparative cohort study. Conclusions: Amongst patients with concurrent meniscus and/or cartilage lesions, patients with BMAC had an 8-point higher mean IKDC score than controls at 9 months postoperatively. However, there was no difference in mean IKDC score between BMAC and control groups if no concurrent pathology was present. Thus, improved IKDC amongst patients with BMAC may be mediated by improved healing of repaired tissue or through an undefined anti-inflammatory or nociceptive pathway. Further investigation will be required to delineate a physiologic mechanism of action associated with BMAC during the recovery process when utilized at the time of ACL allograft reconstruction. [Table: see text][Table: see text][Table: see text]

Poster 288: The Effect of Time Delay From Biopsy to Second-Stage Implantation on Return to Sport and Clinical Outcomes following Autologous Chondrocyte Implantation

Objectives: Expansion of chondral defect size and clinical outcomes in patients with cartilage lesions are thought to be modulated by several risk factors. Previous studies suggest that time delay until implantation may be a risk factor for an increase in the number and size of chondral lesions at the time of implantation. Reports on the effect of time delay on clinical outcomes, however, remain limited. Therefore, the purpose of this study was to evaluate the effect of time delay from cartilage biopsy to implantation on patient satisfaction, return to sport (RTS), and clinical outcomes following autologous chondrocyte implantation. Methods: A retrospective review was performed among patient who underwent autologous chondrocyte implantation (ACI) and matrix-associated autologous chondrocyte implantation (MACI) at a single institution between the years 2012 to 2020 with minimum 2-year clinical follow-up. Clinical outcomes were measured using the Visual Analog Scale (VAS) for pain and satisfaction and the Knee Injury and Osteoarthritis Outcome Score (KOOS; subscores: symptoms, pain, sport, and quality of life (QOL)). Time delay was calculated as the elapsed time between cartilage biopsy and implantation. Cartilage lesions were categorized based on the Outerbridge classification of chondral lesions. The number of high-grade lesions (Outerbridge grade 3 or 4) and size of lesions were analyzed at time of biopsy and implantation. T-tests or non-parametric tests and Chi-squared tests were used to analyze continuous variables and categorical variables, respectively. A one-way ANOVA was used to compare outcomes based on RTS status, using a post-hoc Tukey’s HSD test for multiple comparisons. Bivariate linear regression was used to determine the impact of the time interval from biopsy to implantation on the change in size of the largest high-grade lesion and RTS. Results: A total of 36 patients, mainly female (55.6%) aged 29.25 (± 9.49) with a mean follow-up of 6.51 ± 2.5 years were included in our analysis. Thirteen patients (36.1%) did not RTS. Of the 23 patients (63.9%) who returned to sport, 11 (30.6%) returned at their preoperative level or higher, and 12 (33.3%) returned at a lower level. VAS pain scores were significantly higher in patients who did not RTS vs those who did (42.85 vs. 9.35, p &lt;0.001). All KOOS subscores (Symptom: 56.54 vs. 81.96, Pain: 72.22 vs. 91.74, Sports: 40.38 vs. 79.3, QOL: 35.58 vs. 70.38, p &lt;0.001) were significantly lower in patients who did not RTS compared to those who returned. Increase in time delay from biopsy to implantation was associated with an increase in VAS pain score (OR [95% CI]; 1.42 [0.16 to 2.69], p=0.02) and inversely related to KOOS pain scores (OR [95% CI]; -1.01 [-1.83 to -0.01], p= 0.01. Furthermore, when comparing time from biopsy to implantation and expansion of the largest highgrade lesion, it was found that for every month increase in time, lesion surface area increased by 0.588 cm2; however, this correlation did not reach significance (p=0.09). The number of high-grade lesions at time of implantation were significantly higher in those that returned to sport at a lower level than those who did not return (1.67 vs. 1.23, p=0.02). The average surface area of the defect at biopsy and implantation were 3.13 cm2 and 4.38 cm2 respectively. The number of patients who had an increase in the number of high-grade lesions from biopsy to implantation was significantly higher in those who returned to sport at a lower level compared to those who did not return (4 vs. 1, p=0.05). Logistic regression demonstrated no significant relationship between time interval and rate of return to sport (p=3.59). No other associations were observed between time from biopsy to implantation and clinical outcomes. Conclusions: An increase in time delay from biopsy to graft implantation is associated with worse pain after surgery. Both the number of high-grade lesions at the time of implantation and an increase in time delay were not associated with lower rates of return to sport. Further studies are needed to evaluate the effects of time delay until implantation and its potential role as a risk factor for worse clinical outcomes in patients undergoing autologous chondrocyte implantation procedures. [Table: see text]

Knee- and Overall Health-Related Quality of Life Following Anterior Cruciate Ligament Injury: A Cross-sectional Analysis of Australian and Canadian Cohorts.

OBJECTIVE: To describe the knee- and overall health-related quality of life (QOL) 3 to 12 years after anterior cruciate ligament (ACL) tear, and to assess the association of clinical and structural features with QOL after ACL tear. DESIGN: Cross-sectional analysis of combined data from Australian (n = 76, 5.4 years postinjury) and Canadian (n = 50, 6.6 years postinjury) prospective cohort studies. METHODS: We conducted a secondary analysis of patient-reported outcomes and index knee magnetic resonance imaging (MRI) acquired in 126 patients (median 5.5 [range: 4-12] years postinjury), all treated with ACL reconstruction. Outcomes included knee (ACL Quality of Life questionnaire [ACL-QOL]) and overall health-related QOL (EQ-5D-3L). Explanatory variables were self-reported knee pain (Knee Injury and Osteoarthritis Outcome Score [KOOS-Pain subscale]) and function (KOOS-Sport subscale), and any knee cartilage lesion (MRI Osteoarthritis Knee Score). Generalized linear models were adjusted for clustering between sites. Covariates were age, sex, time since injury, injury type, subsequent knee injuries, and body mass index. RESULTS: The median [range] ACL-QOL score was 82 [24-100] and EQ-5D-3L was 1.0 [-0.2 to 1.0]. For every 10-point higher KOOS-Sport score, the ACL-QOL score increased by 3.7 points (95% confidence interval [CI]: 1.7, 5.7), whereas there was no evidence of an association with the EQ-5D-3L (0.00 points, 95% CI: -0.02, 0.02). There were no significant association between KOOS-Pain and ACL-QOL (4.9 points, 95% CI: -0.1, 9.9) or EQ-5D-3L (0.05 points, 95% CI: -0.01, 0.11), respectively. Cartilage lesions were not associated with ACL-QOL (-1.2, 95% CI: -5.1, 2.7) or EQ-5D-3L (0.01, 95% CI: -0.01, 0.04). CONCLUSION: Self-reported function was more relevant for knee-related QOL than knee pain or cartilage lesions after ACL tear. Self-reported function, pain, and knee structural changes were not associated with overall health-related QOL. J Orthop Sports Phys Ther 2023;53(7):1-12. Epub: 8 June 2023. doi:10.2519/jospt.2023.11838.

Poster 119: MRI evidence of labral tears in healthy young athletes without hip pain

Objectives: The prevalence of labral tears in asymptomatic active adults has been reported, but the prevalence of labral tears and other incidental hip lesions in the asymptomatic adolescent population has not yet been determined. The purpose of this study was to determine the prevalence of hip abnormalities detected on 3T MRI in an active adolescent population with no hip symptoms and, secondarily to compare with hip abnormalities found in adolescents who underwent an MRI for a hip related condition. Methods: Following IRB approval, adolescent patients presenting with isolated knee pain requiring knee MRI were prospectively recruited to have a 3T MRI of their asymptomatic contralateral hip (ASx). A comparison group of adolescent subjects who underwent an MRI for hip pain was enrolled retrospectively (Sx). All MRI scans were anonymized and randomized. Based on preliminary power analysis, 50 subjects were enrolled for each cohort. Blinded to the patient’s cohort status, clinical and demographic data, 2 fellowship-trained musculoskeletal radiologists evaluated these MRI’s independently for abnormal hip lesions, including labral tears. Inter-reader reliability was evaluated using Cohen’s Kappa. A chi-square or Fisher’s exact test was used to compare the prevalence of these hip lesions between the two cohorts. Results: Average patient age was 14.9 years for both cohorts (range 9-18 years); 52% were female. In the ASx group, incidental labral tears were found in 18%, labral/paralabral cysts 6%, cartilage lesion 0%, subchondral cyst 0%, ligamentum teres tear 0%, femoral fibrocystic change 0%, cam lesion 30%, acetabular bone edema 0%, acetabular rim fracture 0%. The prevalence of labral tears (30%, P-value 0.160) and cam lesion (36%, P-value 0.523) in the Sx group was not significantly different from that of the ASx cohort. Conclusions: Labral tears were present on 3T MRI’s of adolescents with and without hip pain. While MRI is essential to confirm the surgeon’s suspicion and to detect unexpected pathology, clinical exam and history are crucial in pinpointing clinically relevant abnormal imaging findings. [Table: see text]

Poster 126: Chronic ACL Injury Patients Have Lower Levels of Pro-Inflammatory Biomarkers in their Synovial Fluid and Worse ACLOAS Score at the Time of Surgery Than Those with Acute ACL Injury: A Cohort Study

Objectives: The relationship between structural abnormalities and inflammatory changes in the synovial fluid following ACL injury has not been well defined. The purpose of this study is to compare MRI findings and concentration of inflammatory biomarkers in the synovial fluid (SF) of acute and chronic anterior cruciate ligament (ACL) injury patients. Methods: Patients undergoing surgery for ACL injury from October 2011 to December 2021 were included in a database of patients who had SF samples collected at the time of surgery. From this cohort, 82 patients with acute ACL injury and 28 patients with chronic ACL injury were identified. All patients underwent a preoperative MRI examination of the knee and SF was aspirated from the operative knee prior to the surgical incision and concentrations of inflammatory biomarkers were quantified. MRI examinations were reviewed by a fellowship-trained musculoskeletal radiologist to assess radiographic features of acute and chronic knee injury using the validated ACLOAS system. Nonparametric tests were used to compare demographic factors, MRI findings, clinical symptoms, and SF inflammatory biomarkers between acute versus chronic ACL injury patients. A generalized linear model was used to assess the relationship of each SF biomarker with the ACLOAs subscores. Results: For acute ACL injury patients, mean time from injury to MRI and injury to surgery was 14 ± 35 days and 49 ± 38 days, respectively. The mean time from injury to MRI and injury to surgery was 3.2 ± 3.7 years and 3.4 ± 3.8 years, respectively, for those with chronic ACL injury. There were no significant demographic differences between groups; age (acute: 31.62 ± 8.02 years, chronic: 33.70 ± 10.60 years, p=0.130), sex (acute: 51.2% males, chronic: 57.1%, p=0.588), or body mass index (acute: 5.5 ± 4.4 kg/mm2, chronic: 25.3 ± 3.6 kg/mm2, p=0.931). Acute ACL injury patients had higher bone marrow edema severity scores (p&lt;0.001), Hoffa’s synovitis score (p=0.002), and effusion severity scores (p&lt;0.001). Chronic ACL injury patients had higher cartilage degeneration severity scores (p&lt;0.001), prevalence of meniscus tears (p=0.003), and meniscus tear severity scores (p&lt;0.001). Acute ACL injury patients had significantly higher synovial fluid levels of IL-6 (p=0.032), VEGF (p=0.002), TIMP1 (p&lt;0.001), IL1-RA (p=0.003), and MMP3 (p&lt;0.001) than chronic ACL injury patients. The generalized linear model revealed that after adjusting for age, sex, BMI, and time from injury to surgery, increased VEGF (p=0.003) and MMP3 (p=0.012) were associated with higher effusion score. MMP-3 was associated with a higher bone marrow edema score (p=0.034), and RANTES was associated with both a higher meniscus severity score (p=0.027) and increased cartilage lesions in the chronic ACL injury cohort. Conclusions: The findings from this study suggest that despite having more severe cartilage degeneration and a higher prevalence of meniscus pathology, chronic ACL injury patients had lower levels of synovial fluid inflammatory biomarkers at time of surgery compared to acute ACL injury patients. Additionally, we found that in chronic injuries, RANTES was associated with worse ACLOAS cartilage scores.

Poster 115: Patients who Underwent Primary Hip Arthroscopy for Femoroacetabular Impingement with Acetabular Microfracture Show 77% Survivorship at 10-Year Follow-Up

Objectives: The purpose is to report minimum 10-year follow-up survivorship, defined as non- conversion to total hip arthroplasty (THA), and patient-reported outcome scores (PROS) following primary hip arthroscopy with acetabular microfracture in the setting of femoroacetabular impingement syndrome (FAIS) and acetabular chondral lesions, respectively. Methods: Data was prospectively collected and retrospectively analyzed on all patients who underwent a primary hip arthroscopy and received an acetabular microfracture between June 2009 and January 2011 and included in this study. Patients with a minimum 10-year follow-up for the modified Harris Hip Score (mHHS), Nonarthritic Hip Score (NAHS), and the visual analog scale (VAS) for pain were included. If available, the minimum 10-year follow-up for the Hip Outcome Score-Sport-Specific Subscale (HOS-SSS) was reported. The demographics, intraoperative findings, surgical procedures, PROS, rate of achieving the minimal clinical important difference (MCID), and secondary surgeries were analyzed and reported. Results: Twenty-two hips (20 patients) were included in the study, and the mean follow-up time was 124.5 ± 2.2 months. There were 17 hips (77.3%) from males and 5 hips (22.7%) from females. The average patient age at the time of surgery was 42.3 years ± 9.6. All patients on average experienced statistically significant improvement (P &lt; 0.05) between preoperative and minimum 10-year follow-up scores for all PROs. In total, 77.3% of the patients did not require conversion to THA. Additionally, 83.3% of the patients achieved the MCID for the mHHS, NAHS, and VAS for pain. Conclusions: At a minimum 10-year follow-up, survivorship of 77.3% was reported for patients that underwent primary hip arthroscopy with acetabular microfracture for the treatment of FAIS and focal/full-thickness acetabular cartilage lesions. Further, in the patients that did not require THA conversion, significant improvement in all PROS was demonstrated.

Review of Surgical Management Techniques for Osteochondral Lesions.

Early attempts at surgical management of cartilage lesions date back to the 1950s. Since then, various reconstructive techniques have been developed; unfortunately, none of the methods used has been able to produce a regenerate formed solely of hyaline cartilage. This paper summarizes the most popular techniques for chondral and osteochondral reconstructions of knee joint tissues.The techniques differ in their indications, which depend primarily on the location of the injury, the extent of the damage and the patient's overall health. In cases of deep damage, osteochondral reconstruction is indicated, which involves both repairing the bone defect and creating favorable conditions for the formation of regenerative tissue cartilage.The use of an appropriate repair technique increases the chances of a good therapeutic effect, which is understood as a reduction in pain, resumption of previous activities and slowing down the progression of osteoarthritis.

Patient-Specific Instrumentation for Medial Closing Wedge Distal Femoral Osteotomy With Patellar Osteochondral Allograft

The primary indications for performing a medial closing wedge distal femoral osteotomy are valgus knee malalignment, lateral knee compartment overload, lateral meniscus insufficiency, and/or lateral compartment osteoarthritis or cartilage damage. Without correction of this malalignment, there is an increased risk for chondral damage in the lateral and patellofemoral compartment of the knee. The optimal candidates for this procedure are young, active individuals with moderate to severe arthritis in the lateral compartment. Recently, preoperative planning for high tibial and distal femoral osteotomies (HTOs and DFOs) using 3-dimensional (3D) patient-specific instrumentation (PSI) has increased in popularity. Successful patient outcomes have been reported using this technique. This Technical Note illustrates our preferred technique that uses 3D PSI in addition to a patellar OCA transplant when treating a symptomatic cartilage lesion associated with genu valgum.

Comparative evaluation of autologous platelet-rich plasma and platelet lysate in patients with knee osteoarthritis

Autologous platelet-rich plasma (PRP) and platelet lysate (PL) are nowadays promising candidates in the treatment of articular cartilage lesions. We aimed to compare PRP and PL injection effectiveness in patients with knee osteoarthritis (KOA). A total of fifty women with KOA were included in the study. Patients were treated with intra-articular injections of PRP and PL. Clinical outcomes were evaluated using the comparison of VAS, WOMAC, and ROM scores. The concentration levels of growth factors and cytokines were measured by ELISA. All patients showed significant improvements in pain and function following treatment of KOA with PL and PRP compared to baseline. Moreover, PL's concentration of growth factors was significantly higher than PRP. A significant increase was also observed in all of the aforementioned mediators in both PRP and PL products compared to control. These results can introduce PL as a promising and alternative option for KOA therapy in the future.

Patellofemoral joint geometry and osteoarthritis features 3-10 years after knee injury compared with uninjured knees.

In this cross-sectional study, we compared patellofemoral geometry in individuals with a youth-sport-related intra-articular knee injury to uninjured individuals, and the association between patellofemoral geometry and magnetic resonance imaging (MRI)-defined osteoarthritis (OA) features. In the Youth Prevention of Early OA (PrE-OA) cohort, we assessed 10 patellofemoral geometry measures in individuals 3-10 years following injury compared with uninjured individuals of similar age, sex, and sport, using mixed effects linear regression. We also dichotomized geometry to identify extreme (>1.96 standard deviations) features and assessed likelihood of having extreme values using Poisson regression. Finally, we evaluated the associations between patellofemoral geometry with MRI-defined OA features using restricted cubic spline regression. Mean patellofemoral geometry did not differ substantially between groups. However, compared with uninjured individuals, injured individuals were more likely to have extremely large sulcus angle (prevalence ratio [PR] 3.9 [95% confidence interval, CI: 2.3, 6.6]), and shallow lateral trochlear inclination (PR 4.3 (1.1, 17.9)) and trochlear depth (PR 5.3 (1.6, 17.4)). In both groups, high bisect offset (PR 1.7 [1.3, 2.1]) and sulcus angle (PR 4.0 [2.3, 7.0]) were associated with cartilage lesion, and most geometry measures were associated with at least one structural feature, especially cartilage lesions and osteophytes. We observed no interaction between geometry and injury. Certain patellofemoral geometry features are correlated with higher prevalence of structural lesions compared with injury alone, 3-10 years following knee injury. Hypotheses generated in this study, once further evaluated, could contribute to identifying higher-risk individuals who may benefit from targeted treatment aimed at preventing posttraumatic OA.