Carrot Oil Research Articles

Use of Carrot Oil, Olive Oil, Pine Oil, Rose Oil in Comparison to Xylene as Clearing Agent During Tissue Processing: an in Vitro Study

ABSTRACT: Background: Clearing plays a vital role in histological section preparation. Xylene has long been valued for its ability to efficiently deparaffinize and clear tissue samples. However, its hazardous properties have raised health and environmental concerns, leading researchers to seek alternative solvents for tissue processing. This study utilized safer substitutes to address these concerns. Aim: To assess the clearing ability of four different oils i.e., Carrot oil, Olive oil, Pine oil, and Rose oil in comparison with that of xylene. Materials and Methods: Thirty different formalin-fixed tissue samples were cut into five parts and cleared using Carrot oil, Olive oil, Pine oil, Rose oil, and xylene followed by routine processing, sectioning, and staining steps. Presence or absence of shrinkage, ease of cutting, nucleus, cytoplasm details and staining quality were assesed and comparison was done between each of them cleared with the 4 different oils with that cleared using xylene. Results: The results showed that all four oils cleared tissues as effectively as xylene, with Pine oil superior in nucleus and cytoplasm details, as well as staining quality insignificant number of samples. Conclusion: These oils not only more affordable and eco-friendly than xylene but can also serve as effective clearing agents.

Effect of Thermally Extracted Carrot Oil Using Biomass Solvent to Boost Consumer Goods: Box-Behnken Design Approach

Massive production of fast-moving consumer goods boosts the economy of any nation. Thus, the need to look into ways of accelerating and transforming carrot utilization into more commercialized usage in order to add value to the developmental strides of any nation where carrot is cultivated like Nigeria. This study concluded that the optimization of the carrot oil extracted from crushed carrot and coconut oil (biomass volume) via soxhlet equipment using Box-Behnken Design (BBD) of Response Surface Methodology (RSM) approach, generated an ideal optimal output of 18.58 wt% at SW of 60 g, BV of 200 ml and ET of 14mins. Although, three input elements which are sample weight (SW), biomass volume (BV), and extraction time (ET) were modelled to generate 17 setups by BBD, but standard order (Std) 8 from the 17 setups generated an ideal output of 18.58 wt%. Thus, the process optimization of the extraction process via BBD of RSM showed that both the coefficient of determination (R2) which is 0.9886, and the difference between adjusted R2 and predicted R2 which is less than 0.2 fall within an ideal range. Also, the elements considered for modeling are mutually significant with the quadratic model equation and the interactive effects of the independent parameters on the carrot oil output showed that an increase in the sample weight and time led to a corresponding increase in the carrot oil output, and this is corroborated by the perturbation plot. Additionally, the physiochemical valuables of the carrot oil generated showed that the free fatty acid and the acid value were very low signifying that the carrot oil is edible and can be used in the manufacturing process using the saponification method in making soap and moisturizing cream for the benefit of mankind.

Seed Oils as Tyrosinase Inhibitors for the Treatment of Hyperpigmentation

Background: Hyperpigmentation is a growing problem worldwide among various skin diseases and directly relates to the quality of life. The factors causing hyperpigmentation may range from excess exposure to sunlight, pollution, underlying disease conditions, adverse effects of a medication, modified lifestyle, and oxidative stress. Treatment includes the use of hydroquinone, retinoids, corticosteroids, and kojic acid along with anti-inflammatory drugs. However, these medications are preferred for short-term treatment under acute conditions, while in the case of long-term treatment, botanical extracts are a safe choice for a majority of the population for the treatment of hyperpigmentation. Formulation with plant extracts/oil enriched with polyphenols, vitamin C, and vitamin E are reported to be safe and effective in inhibiting melanogenesis. Objective: Seed oils composed of polyphenols, vitamins and unsaturated fatty acids were selected to evaluate their antioxidant property and tyrosinase enzyme inhibitory activity. Methods: Grape seed oil (GSO), papaya seed oil (PSO), and carrot seed oil (CSO) were evaluated for their total phenolic and flavonoid content , in-vitro antioxidant activity was done using DPPH assay and anti-tyrosinase activity was done usingMushroomtyrosinaseinhibition assay. Result: Total phenolic content for PSO was 0.936 mg/gm. Gallic acid equivalent (GAE) was highest when compared to GSO and CSO, while CSO had a higher total flavonoid concentration, 0.945 mg/gm as quercetin equivalent (QE). Free radical scavenging activity was comparable to standard and tyrosinase inhibitory activity for grape and carrot seed oils were 80.10 % and 76.52 % at 100µg/ml, respectively and was comparable to kojic acid used as standard. Conclusion: The results obtained suggest that the oils can be formulated as a topical depigmenting product and used for skin care and skin glow

Effectiveness Of Cream Formulation Of Carrot Seed Oil As Anti-Aging

Carrot (Daucus carota L.) is a vegetable plant that has many uses for public health services in the world. Fresh carrots contain water, protein, carbohydrates, fat, fiber, ash, anti-cancer nutrients, natural sugars (fructose, sucrose, dextrose, lactose, and maltose), pectin, glutathione, minerals (calcium, phosphorus, iron, potassium, sodium, magnesium, chromium), vitamins (beta carotene, B1, and C) and asparagine. The purpose of this study was to characterize simplicia and carrot seed oil extract, and to determine the antioxidant activity of carrot seed oil extract with formulations of concentrations of 3%, 6% and 9% seeds on the effectiveness of anti-aging Moisture, Evenness, Pore, Spot and Wrinkle. Carrot seed oil can be formulated in a homogeneous cream dosage form with an oil-in-water emulsion type, pH 6.7-7.7; does not cause skin irritation and is stable on storage for 90 days at room temperature. Carrot seed oil cream with a concentration of 9% showed the best anti-aging effectiveness by increasing water content, reducing skin hardness, shrinking pores, reducing blemishes and reducing wrinkles compared to other cream formulas.

Could the high incidence of breast cancers in Nigerian women be related to 1,4-Dioxane in skin lightening products?

Two monstrous female health-related issues are currently engaging the attention of the global community. These are the chemical skin-lightening issue and the rising female breast cancer issue. Nigeria has the highest prevalence of chemical skin lightening practice with 77.3% of traders in Lagos using such products and one of the highest incidences of breast cancer globally of 50.5 per 100,000 women. 1,4-Dioxane, a possible contaminant in toiletries and cosmetics, is an IARC group 2B carcinogen and a potential breast carcinogen. It is generated when ethylene oxide -a known breast carcinogen is added to other chemicals during manufacturing. The skin lightening procedure is most conducive for optimal per-cutaneous absorption and inhalation of Dioxane. The research question entertained was if there is a link in the increasing prevalence of skin lightening practice and the rising breast cancer burden in Nigeria. One hundred skin lightening products procured from across Lagos, Nigeria had their listed contents scrutinized and entered onto a spreadsheet, then they were analyzed for Dioxane using Scalia’s Solid Phase Extraction gas chromatography procedure. Water (Aqua) 66%, Glycerin 60% and Tocopherol or Vitamin E 41% were found to be the most listed ingredients on the package labels, with Vitamin C 28%, Kojic acid 26%, Citric acid 22% and Carrot oil seen in 20% of products. All the analyzed samples were negative for Dioxane. The study brought to the fore the need to explore other potential breast carcinogens in skin lightening cosmetics and environmental chemicals.

THE USE OF CARROT SEED OIL (DAUCUS CAROTA L.) TO FORMULATE NANOEMULGELS AS AN EFFECTIVE NATURAL SUNSCREEN AND SKIN ANTI-AGING

Objective: The purpose of this study was to develop a nanoemulgel containing vegetable oil of carrot seed oil as an effective natural sunscreen and skin anti-aging. Methods: Nanoemulgels containing 4% carrot seed oil were formulated in three formulas with different ratios of Tween 80 and Sorbitol and prepared by using the high-energy emulsification method. The nanoemulgels were determined for the organoleptic characteristic, globule size, pH, physical stability during storage for 12 w at three different temperatures (room, high and low temperature), centrifugation, and cycling test. The Sun Protection Factor (SPF) value was determined by UV spectrophotometric method and the effectiveness of anti-aging was evaluated by using a skin analyzer and the results were compared with sunscreen emulgel. Results: Nanoemulgel containing 4% carrot seed oil with a ratio of Tween 80 as surfactant and Sorbitol as co-surfactant 40 and 20 resulted in the smallest mean droplet size of 338.34 nm and the sizes were increased during 12 w of storage at room temperature but still in the nano size and this nanoemulgel did not show phase separation or still stable. These nanoemulgels were also stable after the centrifugation and cycling test. The emulgel preparation was not stable or showed phase separation after the centrifugation test. The SPF value obtained from the nanoemulgel was 20.28±0.22 and these values were higher than the sunscreen emulgel (13.94±0.27). The pore size, spot, and wrinkles of the volunteer skin were reduced after using the nanoemulgel containing 4% carrot seed. Conclusion: The sunscreen and skin anti-aging activity of nanoemulgel preparation containing 4% carrot seed oil with a ratio of surfactant Tween 80 and co-surfactant Sorbitol 40 and 20 were more effective compare with emulgel preparation.

Inhibitory Potential of Coconut and Carrot Oil Extracts on Some Bacterial Skin Microflora

In response to the chemically harmful effects of commercially sold body creams on the skin due to the presence of steroids, hydroquinone and kojic acid, natural oil extracts from medicinal plants and fruits are seen as alternative. The use of natural oil extract is not without some intricacies too as its effect against skin microflora has been reported. Therefore, the inhibitory potentials of coconut and carrot oil extracts on some bacterial skin microflora were determined using standard laboratory identification and agar well diffusion methods for identification of bacteria from human skin swab and antibacterial susceptibility test, respectively. The isolated organisms include, Staphylococcus aureus, Streptococcus species, and Pseudomonas aeruginosa. The result obtained shows that carrot oil extract inhibits all the test isolates with mean zones of inhibition (MZI) ranged from 6.00±1.00 to 12.15±0.55 while coconut oil extract was only active against Pseudomonas aeruginosa with MZI of 12.50±0.55. The minimum inhibitory concentration (MIC) result also shows that carrot oil extract was significantly (p ˂ 0.05) more active than coconut oil with MIC of 50% while the test isolates were still viable at 100% use of coconut oil extract. There was no minimum inhibitory concentration (MBC) obtained as all the test organisms were still viable at 100% of both oil extracts. From the result of this study, it can be concluded that carrot oil extract possesses inhibitory potentials against skin microflora as opposed to coconut oil extract. Therefore, it is safer to apply coconut oil on human skin since it has less inhibitory effect on skin microflora.

Triacylglycerols and Fatty Acid Compositions of Cucumber, Tomato, Pumpkin, and Carrot Seed Oils by Ultra-Performance Convergence Chromatography Combined with Quadrupole Time-of-Flight Mass Spectrometry.

The triacylglycerol (TAG) compositions of cucumber, tomato, pumpkin, and carrot seed oils were analyzed using ultra-performance convergence chromatography (UPC2) combined with quadrupole time-of-flight mass spectrometry (Q-TOF MS). A total of 36, 42, 39, and 27 different TAGs were characterized based on their Q-TOF MS accurate molecular weight and MS2 fragment ion profiles in the cucumber, tomato, pumpkin, and carrot seed oils, respectively. Generally, different vegetable seed oils had different TAGs compositions. Among the identified fatty acids, linoleic acid was the most abundant fatty acid in cucumber, tomato, and pumpkin seed oils and the second most abundant in carrot seed oil with relative concentrations of 54.48, 48.69, 45.10, and 15.92 g/100 g total fatty acids, respectively. Oleic acid has the highest concentration in carrot seed oil and the second highest in cucumber, tomato, and pumpkin seed oils, with relative concentrations of 78.97, 18.57, 27.16, and 33.39 g/100 g total fatty acids, respectively. The chemical compositions of TAGs and fatty acids could promote understanding about the chemical profiles of certain vegetable seed oils, thus improving the potential ability to select appropriate oils with specific functions and a high nutritional value and then develop functional foods in the future.

Extraction and Characterization of Selected Carrier Oils

Extraction and characterization of carrier oil has been conducted experimentally on coconut, avocado and carrot oil. FTIR scan analysis was carried out on the extracted oil. Also physical and chemical properties of the carrier oils were assessed to evaluate oil quality including Specific gravity, peroxide value, saponification value, acid value and free fatty acid. The result showed all the extracted oils were liquid at room temperature an indication of the presence of oleic acid and linoleic acid and other unsaturated fatty acids. The specific gravities of 0.92, 0.91 and 0.93 for coconut, avocado and carrot oils indicate that each of the oils could be used on commercial scale. The Peroxide value recorded 0.11-0.12 meq/kg which is far below the maximum limits of 1-5 meq/kg and can last for a long time without going rancid. The low saponification values of coconut, avocado and carrot oils of 127.62 meq/kg oil, 120.12 meq/kg oil and 130.47 meq/kg oil respectively indicates that the oils could be used industrially (soap making). An iodine value of 54.63g I2/100g coconut oil, 49.21g I2/100g avocado oil and 55.01g I2/100g carrot oil obtained indicates high level of unsaturated fatty acids and can explain its liquid state on storage at room temperature. Acid values of 6.10 mg KOH/g coconut oil, 9.40 mg KOH/g avocado oil and 6.50 mg KOH/g carrot oil indicated that the oils are acceptable for industrial purposes. The % FFA values obtained as 3.10%, 4.70% and 3.30% for coconut, avocado and carrot oils respectively indicates the suitability of the oils for consumption. The FTIR scan analysis showed the various compounds present in the oils and by indication presented that coconut oil have dominant properties over that of carrot oil and should not be used as base oil for its extraction. Carboxylic acid (effective in providing antioxidant and anti-aging protection as well as improving moisture retention) and aromatic groups were found present as well as esters (excellent hydrating and softening agents). These compounds are found in all vegetable oils thereby validating their quality and explain the excellent properties of carrier oils and why they are used in conjunction with essential oils for aromatherapy purposes as well as for human consumption.

Masere Domates (Lycopersicon esculentum) ve Havuç (Daucus carota) Yağlarının Yüksek Yoğunlukta Stoklanmış Gökkuşağı Alabalığı (Oncorhynchus mykiss, L.)’nın Hematolojik Parametrelerine Etkisi

In this study, 2% of macerated tomato (Lycopersicon esculentum) and carrot (Daucus carota) oils were added to the feeds of rainbow trout with an average weight of 35.88 ± 1.97 g. The experiment was continued for 21 days and the samples were also measured for changes in the hematological parameters. Statistically, significant differences were found in LYM, RBC, HGB, HCT, MCH and NBT values (p<0.05). There was no statistically significant difference other blood parameters levels (p>0.05). It was determined that both macerated carrot and macerated tomato oils stimulated to the non-specific immune system and erythropoiesis at high stocking density. Especially, it was occurred that macerated carrot oil (MCO) more effective than macerated tomato oil (MTO).

Bioactivity of Wild Carrot (Daucus carota, Apiaceae) Essential Oil Against Mosquito Larvae.

Invasive alien plants wreak havoc on native ecosystems and using them as a source of biopesticides could improve their management. We examined the toxicity of essential oil of wild carrot (also known as 'Queen Anne's Lace', Daucus carota Linnaeus), an aggressive invader throughout the United States, against Aedes aegypti L., Culex pipiens L., and Culex restuans Theobald larvae. Comparisons were made between essential oil extracted from umbels of local populations of wild carrot versus a commercial brand. Methyl isoeugenol (60.7%) was by far the most abundant constituent in commercial brand oil, whereas α-pinene (33.0%) and β-pinene (25.8%) were the dominant constituents in essential oil extracted from local wild carrot populations. The commercial brand essential oil was significantly more toxic to Cx. restuans larvae (LC50 = 44.4 ppm) compared with Cx. pipiens (LC50 = 51.0 ppm) and Ae. aegypti (LC50 = 54.5 ppm). Essential oil from local populations of wild carrot was significantly more toxic to both Cx. pipiens (LC50 = 42.9) and Cx. restuans (LC50 = 40.3) larvae compared with Ae. aegypti (LC50 = 64.6 ppm) larvae. Three of the nine tested chemical constituents of wild carrot essential oil (terpinolene, para cymene, and γ-terpinene) were consistently more toxic to larvae of the three mosquito species than the whole essential oil. These findings suggest that exploiting wild carrot essential oil and its chemical constituents as a biopesticide for mosquito control could be used as part of multifaceted approaches for controlling this invasive alien plant species.

Możliwości wykorzystania w kosmetologii wybranych olejów roślinnych jako źródła karotenoidów

Introduction. Plant oils, due to the content of polyunsaturated fatty acids, which have a positive effect on the condition of the skin, are widely used in cosmetology. Some of them are also used as a rich source of carotenoids, plant dyes, which enjoy unmatched interest of cosmetics manufacturers because of their properties (color, physiological activity). Aim. The aim of the study was to compare the total carotenoid content, including β-carotene, in selected plant oils applied in cosmetology. Material and methods. The carotenoid content in sea buckthorn (Hippophaë rhamnoides L.), carrot (Daucus carota L.), marigold (Calendula officinalis L.) and pumpkin (Cucurbita pepo L.) was determined by UV/VIS spectrophotometry and HPLC methods. Results. The tested plant oils have a different content of plant dyes. In terms of total carotenoid content, including β-carotene, they can be arranged as follows: sea buckthorn oil > carrot oil > marigold oil > pumpkin seed oil. Conclusions. The results of this study show that the tested plant oils, especially sea buckthorn oil, can be used in cosmetic formulations as a source of carotenoids, including β-carotene.

Wild carrot pentane-based fractions suppress proliferation of human HaCaT keratinocytes and protect against chemically-induced skin cancer

BackgroundPrevious studies in our laboratory showed that the Lebanese Daucus carota ssp. carota (wild carrot) oil extract possesses in vitro and in vivo anticancer activities. The present study aims to examine the cytotoxic effect of Daucus carota oil fractions on human epidermal keratinocytes and evaluate the chemopreventive activity of the pentane diethyl ether fraction on DMBA/TPA induced skin carcinogenesis in mice.MethodsWild carrot oil extract was chromatographed to yield four fractions (F1, 100% pentane; F2, 50:50 pentane:diethyl ether; F3, 100% diethyl ether; F4 93:7 chloroform:methanol). The cytotoxic effect of fractions (10, 25, 50 and 100 μg/mL) was tested on human epidermal keratinocytes (non-tumorigenic HaCaT cells and tumorigenic HaCaT-ras variants) using WST a ssay. Cell cycle phase distribution of tumorigenic HaCaT-ras variants was determined by flow cytometry post-treatment with F2 fraction. Apoptosis related proteins were also assessed using western blot. The antitumor activity of F2 fraction was also evaluated using a DMBA/TPA induced skin carcinoma in Balb/c mice.ResultsAll fractions exhibited significant cytotoxicity, with HaCaT cells being 2.4–3 times less sensitive than HaCaT-ras A5 (benign tumorigenic), and HaCaT-ras II4 (malignant) cells. GC-MS analysis revealed the presence of a major compound (around 60%) in the pentane/diethylether fraction (F2), identified as 2-himachalen-6-ol. Treatment of HaCaT-ras A5 and HaCaT-ras II4 cells with F2 fraction resulted in the accumulation of cells in the sub-G1 apoptotic phase and decreased the population of cells in the S and G2/M phases. Additionally, F2 fraction treatment caused an up-regulation of the expression of pro-apoptotic (Bax) and down-regulation of the expression of anti-apoptotic (Bcl2) proteins. A decrease in the phosphorylation of AKT and ERK was also observed. Intraperitoneal treatment with F2 fraction (50 or 200 mg/kg) in the DMBA/TPA skin carcinogenesis mouse model showed a significant inhibition of papilloma incidence (mice with papilloma), yield (number of papilloma/mouse) and volume (tumor relative size) at weeks 15, 18 and 21.ConclusionThe present data reveal that F2 fraction has a remarkable antitumor activity against DMBA/TPA-induced skin carcinogenesis, an effect that may be mediated through inhibition of the MAPK/ERK and PI3K/AKT pathways.

Carrot Plant-A Potential Source of High Value Compounds and Biological Activities: A Review

Essential oil of carrot is produced and accumulated in the secretary ducts known as vittae. Phytochemical studies have shown that the major chemical components of carrot plant are polyphenols, flavonols, glycosides, alkaloids, saponins, sesquiterpenoids and essential oil. Chemical constituents of carrot seed essential oil obtainedby hydrodistillation have been the subject of frequent researches. The composition of essential oil varies with plant parts. The carrot essential oil possesses biological activities like antimicrobial, antibacterial, antifungal, herbicidal, antioxidant, anticancer etc. The health benefits of carrot essential oil can be attributed to its antiseptic, disinfectant, detoxifying, antioxidant, anticarcinogenic, tonic, carminative, depurative, diuretic and stomachic properties.Major components present in different parts of carrot plant are listed along with their chemical composition. The morphological details of plant, its essential oil, chemicalconstituents and biological activities have been discussed in this review.

Chemical Transformations of Carotol Isolated from Carrot Seed Oil

Chemical Transformations of Carotol Isolated from Carrot Seed Oil

Carotol: A Sesquiterpenoid Isolated from Carrot Seed Oil

Carotol: A Sesquiterpenoid Isolated from Carrot Seed Oil

Cytotoxic effect of wild carrot oil fractions on human epidermal keratinocytes

Wild carrot has been used in traditional medicine in Lebanon to treat several diseases. Recently, Daucus carota oil extract and its fractions, F1 (pentane; 100%), F2 (pentane-diethyl ether; 50:50), F3 (diethyl ether; 100%) and F4 (chloroform-methanol; 93:7), were shown to possess anticancer and antioxidant activities [1,2]. The present study aims to complete spectrum of the anticancer activity of the 4 fractions against three types of human epidermal keratinocytes (HaCaT cells) cell lines HaCaT-II4 (non-invasive), HaCatT-A5 (invasive) and HaCaT (immortalized). Cells were treated with 10, 25, 50 and 100 µg/mL of the fractions for 48h and cell survival was determined using WST-1 assay. The 4 fractions exhibited a dose-dependent inhibition of cell viability, with F1 and F2 being more potent (p < 0.05) than F3 and F4. The immortalized HaCat cell line was more resistant to cytotoxicity compared with the two malignant counterparts (p < 0.05). The IC50 values of F1, F2, F3, and F4 fractions in the immortalized HaCat cells were respectively 34, 37, 50 and 45 µg/mL, which were significantly (p < 0.05) higher than that of HaCat-A5 cells with IC50 values of 26, 32, 38 and 36 µg/mL respectively and of HaCat-II4 cells with IC50 values of 26, 24, 39 and 34 µg/mL. In conclusion, the results show that F1 and F2 are more toxic to invasive and non-invasive HaCaT than the immortalized cells. Further studies are needed to isolate and characterize the biologically active compounds in F1 and F2.

Chemopreventive potential of the pentane-based fraction of wild carrot oil against chemically-induced squamous cell carcinoma

Chemopreventive potential of the pentane-based fraction of wild carrot oil against chemically-induced squamous cell carcinoma

In vivo immunomodulatory effect of wild carrot oil and its fractions

Daucus carota L. ssp. carota (wild carrot) oil extract (DCOE) and fractions (F1, F2, F3 and F4) were recently shown to exhibit in vitro and in vivo anticancer activity [1,2]. The aim of this study is to examine the in vivo immunomodulatory effect of DCOE and its fractions on the levels of IFN-γ, TNF-α, IL-10, IL-17, and VEGF in spleen tissues of BALB/c mice. Animals were injected with 200 mg/Kg of DCOE or fractions and spleens were collected at 6 and 24 hrs post treatment. Whereas, DCOE and F1 did not induce a significant increase in IFN-γ levels at either interval, F2, F3, F4 and LPS caused 6.7, 4.6, 3.5 and 2.3 fold increases, respectively. The level of TNF-α displayed 2.4, 2.8, 6.2, 3.8, 2.5 and 3.1 fold increase compared to control 24 hrs post treatment with DCOE, F1, F2, F3, F4 and LPS, respectively. DCOE, F1 and F4 induced a 1.3 fold increase and F2 and F3 induced a 1.4 fold increase in the IL-10 levels, 6 hrs post treatment. However, no significant elevation of IL-10 was observed 24 hrs post injection of F2, F3 and F4 fractions. While 1.7, 1.6, and 1.75 fold increases in IL-17 were observed 6 hrs post treatment with F2, F3 and F4 respectively, these increments did not reach significance after 24 hrs. In addition, F1 and F2 were shown to inhibit the production levels of spleen VEGF. In conclusion, the in vivo antitumor activity of DCOE and fractions may be attributed to the enhancement of IFN-γ, TNF-α and IL-17 production and down regulation in the level of VEGF.

Bioactivity and safety profile of Daucus carota subsp. maximus essential oil

Daucus carota (carrot) essential oil is one of the most valued by-product of this species due to its medicinal properties. The present study focuses on the antifungal and anti-inflammatory activity of the unexplored essential oil of wild carrot (D. carota subsp. maximus) from Portugal.The essential oil from the aerial parts of plants was analysed by GC and GC–MS. The oil showed high contents of α-pinene (22.2%), geranyl acetate (16.0%), β-bisabolene (11.5%), α-asarone (9.8%) and E- methylisoeugenol (8.0%).The antifungal activity (MIC and MLC) was evaluated against several pathogenic strains. The oil showed a broad antifungal activity being particularly active against dermatophytes and Cryptococcus neoformans, with MIC values ranging from 0.16 to 0.32μL/mL. The anti-inflammatory potential was also assessed at both a peripheral and central level, by measuring nitric oxide (NO) production in lipopolysaccharide-stimulated macrophages and microglia cells. The essential oil (0.32μL/mL) was able to reduce NO production by 20.7% and 35.8% in macrophages and microglia, respectively. Moreover, the oil safety profile was demonstrated in several mammalian cells using the MTT assay.Both bioactive and safe concentrations of D. carota subsp. maximus essential oil were disclosed supporting further in vivo investigations for the development of therapeutic effective complements in the management of dermatophytosis and/or inflammatory-related disorders. Moreover, bearing in mind that inflammation plays a key role in the development of neurodegenerative diseases, the novelty and significance of these results set up an interesting starting point for the development of a nutraceutical to prevent or ameliorate these highly prevalent chronic disorders.