Carrot Extract Research Articles

Application of bacterial nanocellulose film loaded with sodium nitrite, sumac, and black carrot extracts to reduce sodium nitrite, extend shelf life, and inhibit Clostridium perfringens in cooked beef ham

This study aimed to develop innovative bacterial nanocellulose (BNC) films incorporated with sodium nitrite (SN), sumac extract (SE), and black carrot extract (BCE) to reduce sodium nitrite, extend shelf life, and inhibit Clostridium perfringens in cooked beef ham. The ham covered with BNC film immersed in SE (10 % w/v) + BCE (5 % w/v) + SN (125 ppm) (SE10BCE5SN125) exhibited the lowest microbial load (4.39, 4.32, 3.83, and 4.6 log cycles reductions in total viable count, lactic acid bacteria, Enterobacteriaceae, and C. perfringens, respectively) and the lowest oxidation rate (70 % reduction compared to control) on day 28 of storage. The samples covered with SE10BCE5SN125 film had also the lowest level of residual nitrite (64 % lower compered to positive control). Positive control (ham containing 120 ppm SN) and ham covered with SE10BCE5SN125 film had the highest redness. It was concluded that the films developed in this study have the potential to reduce the use of sodium nitrite in beef ham production.

High Specificity and Sensitivity Method for Quantitation of Anthocyanins by Precursor Ion Scan Using Liquid Chromatography‐Tandem Mass Spectrometry Technique

ABSTRACTAnthocyanins, primarily found in flowers, vegetables, and fruits, play a critical role in human and plant health. These polyphenolic pigments are predominantly found in plants in the form of glucosides linked to one or more sugar units and exhibit a broad range of structural diversity. This creates a complex spectrum of anthocyanins making it challenging to perform its absolute quantification in different plant matrices due to the lack of availability of all reference standards. More commonly today, anthocyanins are quantified by spectrophotometric methods, which is non‐specific quantification due to a lack of information on the anthocyanin classes present. In this study, a systematic approach has been proposed to address this challenge, utilizing liquid chromatography‐tandem mass spectrometry using precursor ion scan mode for high‐specificity identification and subsequent quantification of the anthocyanin classes. A method was developed for six anthocyanin classes and it was used to quantify anthocyanins in black carrot extract. The initial scan was conducted for all anthocyanin classes, followed by the quantification of the anthocyanin classes detected using the reference standard specific to that class. This approach has demonstrated that precursor ion scan mass spectrometry can serve as a valuable tool for the rapid identification and specific quantification of anthocyanin classes in plants.

Acylated Anthocyanins From Black Carrots and Their Related Phenolic Acids Diminish Priming and Activation of the NLRP3 Inflammasome in THP-1 Monocytes.

Excessive activation of the nucleotide-binding oligomerization domain-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome contributes to chronic inflammation. Thus, targeting NLRP3 inflammasome activation by anthocyanins may prevent inflammatory diseases. Therefore, the present study determines the influence of a black carrot extract (BCE) with high amounts of acylated anthocyanins and their related phenolic acids on the NLRP3 inflammasome. THP-1 monocytes are pretreated with a BCE, cyanidin-3-glucoside (C3G), or hydroxycinnamic acids. NLRP3 inflammasome assembly is initiated by priming THP-1 monocytes with lipopolysaccharide and/or activating the NLRP3 inflammasome with nigericin. Flow cytometry is used to assess apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) speck formation, as well as ASC and NLRP3 protein expression. Caspase-1 activity is measured using a bioluminescent assay, and cytokine concentrations are determined by enzyme-linked immunosorbent assays (ELISA). C3G and phenolic acids diminish ASC and NLRP3 protein expression. In addition, C3G and phenolic acids attenuate ASC speck formation. Furthermore, the BCE and C3G decline caspase-1 activity. Consistently, IL-1β and IL-18 secretion are reduced upon NLRP3 inflammasome activation. The present study shows that a BCE with high amounts of acylated anthocyanins and their related phenolic acids diminish priming and activation of the NLRP3 inflammasome in THP-1 monocytes.

Investigating the antioxidant and anticancer potential of Daucus spp. extracts against human prostate cancer cell line C4-2, and lung cancer cell line A549

The study evaluated 297 carrot germplasm lines, focusing on 52 cultivars to explore their therapeutic potential and address challenges related to the accessibility and affordability of nutraceuticals and health promoting foods. The investigation explores the application of DNA barcoding using the ITS region for precise species identification, highlighting genetic diversity among the examined cultivars. Through ITS sequence-based analysis and phylogenetic examination, six diverse Daucus spp. genotypes were differentiated and classified into distinct groups, indicating the presence of vast genetic variation. Evaluation of antioxidant activities using the DPPH radical scavenging assay revealed varying degrees of scavenging ability among genotypes with SKAU-C-15, SKAU-C-17, and SKAU-C-16 exhibiting the highest activity, suggesting their potential for antioxidant-rich products. Thin Layer Chromatography (TLC) bioautography confirmed the presence of bioactive compounds in carrot extracts responsible for their antioxidant properties. In cell culture studies, specific carrot genotype extracts demonstrated potential anti-proliferative and anti-invasive effects on recurrent prostate cancer cell line - C4-2 (SKAU-C-30, SKAU-C-10, and SKAU-C-42) and non-small cell lung cancer cell line - A549 (SKAU-C-18 and SKAU-C-11) cancer cells, as indicated by MTT assay, wound healing assay, and Colony Forming Unit assay. These findings suggest the promising therapeutic potential of carrot genotypes for developing anti-cancer functional foods, nutraceuticals and health supplements.Therefore, the study contributes to the nutrition security, paving the way for advancements in functional foods and health applications, particularly in cancer treatment and prevention.

Bilayer pH-sensitive colorimetric indicator films based on chitosan/purple carrot extract and gellan gum/Mg-carbon dots for visual monitoring of pork freshness

A pH-sensitive colorimetric bilayer film was constructed on the basis of layer-by-layer self-assembly, with chitosan (CS)-loaded purple carrot anthocyanin (PCA) as the inner indicator layer and gellan gum (GG)-loaded Mg-doped carbon dots (Mg-CDs) as the protective outer layer. X-ray diffraction and scanning electron microscopy analyses confirmed the successful preparation of the bilayer film and the incorporation of Mg-CDs. Fourier transform infrared spectroscopy indicated that the Mg-CDs formed hydrogen bonds with the film matrix. Thermogravimetric analysis revealed that the Mg-CDs improved the thermal stability of the double-layer films. Moreover, the addition of 7 wt% Mg-CDs increased the water vapor permeability, oxygen permeability, and tensile strength of the films by 0.94-, 0.79-, and 3.18-fold, respectively. The antioxidant and antimicrobial activities of the films were significantly enhanced by including Mg-CDs as the active agents. Illumination experiments revealed that the relative color change (S values) increased by only 2.76 % and 4.69 % after the addition of 7 wt% Mg-CDs to the GG -Mg-CDs layer after 21 days of visible light and 72 h of UV light exposure, respectively. These bilayer films containing PCA and Mg-CDs exhibit good pH color responsiveness and biosafety. The C-P/G-7 %CD film had the best overall performance and can effectively reflect pork freshness during storage. Therefore, this research has great potential for developing a double-layer film for monitoring meat quality changes in intelligent food packaging.

Exploring the Potential of Anthocyanin-Based Edible Coatings in Confectionery-Temperature Stability, pH, and Biocapacity.

This study aims to develop purple-coloured polymeric coatings using natural anthocyanin and desoxyanthocianidins (3-DXA) colourants for application to chocolate almonds. The objective is to achieve a stable and uniform colour formulation throughout processing and storage, enhancing the appearance and durability of the almonds to appeal to health-conscious consumers and align with market demands. Plant materials like sweet potato pulp, sweet potato peel, radish peel, black carrot, and sorghum were employed to obtain the desired purple hue. Anthocyanidins and 3-DXA were extracted from the matrices using solvent extraction and ultrasound-assisted methods at different pH values. High-performance liquid chromatography with diode array detection (HPLC-DAD) and high-resolution tandem mass spectrometry (HRMS/MS) were used to identify the compounds in the extracts. The highest antioxidant capacities, as measured by the DPPH• and FRAP methods, were observed in purple sweet potato and dye factory extracts, respectively; meanwhile, sorghum extract inhibited both α-amylase and α-glucosidase, indicating its potential for managing postprandial hyperglycemia and type 2 diabetes. The degradation kinetics of coloured coatings in sugar syrup formulations with anthocyanins and 3-DXA revealed that locust bean gum offered the best colour stabilization for plant extracts, with sorghum extracts showing the highest and black carrot extracts the lowest colour variation when coated with Arabic gum. Sweet potato pulp extracts exhibited less colour variation in sugar pastes, both with and without blue spirulina dye, compared to factory dye, highlighting their potential as a more stable and suitable alternative for colouring purple almonds, particularly over a five-month storage period. This study supports sustainable practices in the confectionery industry while aligning with consumer preferences for healthier and environmentally friendly products.

Purple Carrot Extract Exhibits a Neuroprotective Profile in th e Nigrostriatal Pathway in the Reserpine-induced Model of Parkinson 's Disease.

Parkinson's disease (PD) is a chronic neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway. Even with scientific and technological advances, the therapeutic approaches used for the treatment of PD have shown to be largely ineffective in controlling the progression of symptoms in the long term. There is a growing demand for the development of novel therapeutic strategies for PD treatment. Different herbs and supplements have been considered as adjuvant to treat the symptoms of Parkinsonism. The carrot is one of the most consumed vegetable species worldwide, and its root is known for its content of anthocyanins, which possess antioxidant and antiinflammatory properties. This study evaluated the neuroprotective effect of purple carrot extract (CAR) in rats on the reserpine (RES)-induced progressive parkinsonism model. Male rats (6-month-old) received orally the CAR (400 mg/kg) or vehicle and subcutaneously RES (0.01 mg/kg) or vehicle for 28 days (Preventive Phase). From the 29th day, rats received CAR or vehicle daily and RES (0.1 mg/kg) or vehicle every other day (for 23 days, Protective phase). Behavioral tests were conducted throughout the treatment. Upon completion, the animals' brain were processed for tyrosine hydroxylase (TH) immunohistochemical assessment. Our results showed that the chronic treatment of CAR protected against motor disabilities, reducing the time of catalepsy behavior and decreasing the frequency of oral movements, possibly by preserving TH levels in the Ventral Tegmental Area (VTA) and SNpc. CAR extract is effective to attenuate motor symptoms in rats associated with increased TH+ levels in the Ventral Tegmental Area (VTA) and SNpc, indicating the potential nutraceutical benefits of CAR extract in a progressive parkinsonism model induced by RES.

Phytochemical characterization and quality evaluation of stirred goat milk yoghurt with added fruit and vegetable puree during storage

PurposeGoat milk yoghurt differs from cow milk yoghurt in that it has a different casein composition and content, which presents several technical challenges, including consistency with an appropriate flavor.Design/methodology/approachIn this study, the antioxidant potential and phytochemical profiling of the fruits (pineapple and papaya) and vegetable (carrot) extracts was evaluated and the effect of their purees on the quality and stability of stirred goat milk yoghurt (GMY) were investigated. The qualities of stirred GMY with carrot (CrY), pineapple (PaY) and papaya (PpY) purees were assessed against the product without puree (CY).FindingsThe carrot puree had the highest moisture, ash contents and pH value. The carrot extract had the highest DPPH radical scavenging activity, while the pineapple extract had the highest total phenolic value (1.59 µg GAE/g) and flavonoids content (0.203 µg CE/g). The scanning of all the puree extracts in GC-MS indicated that 5-hydroxymethylfurfural was a major component. The phytochemical quantification of the extracts through multiple reaction monitoring (MRM) against 16 compounds showed the presence of sinapic acid, cinnamic acid, pthalic acid, ferulic acid, 4-OH-benzoic acid, 3-OH-benzoic acid, p-coumaric acid, caffeic acid and vanillic acid in different quantities. The addition of purees and storage period had a significant (p < 0.05) effect on the moisture, pH, titratable acidity, syneresis, viscosity, color values and sensory properties of the products. In all the samples after 15 days of storage, Streptococcus thermophilus and Lactobacillus bulgaricus counts remained above the recommended level of 106CFU/g. Stirred GMY sample produced with pineapple puree showed a higher syneresis and viscosity, but the CrY sample demonstrated the highest antioxidant activity. The developed formulations remained stable with minimum changes in quality and sensory attribute during refrigerated storage for 10 days.Originality/valueThis study suggests that addition of fruit and vegetable improve the viscosity and sensory perception of the product with minimal use of synthetic flavor and preservatives.

Novel tributyl phosphate-based deep eutectic solvent: Application in microwave assisted extraction of carotenoids

A contribution to the use of deep eutectic solvents (DES) and microwave-assisted extraction (MAE) was made for bioactive compounds recovery, especially those with lipophilic character, from tomato and carrot samples rich in carotenoids. For the first time, a novel deep eutectic solvent was synthesized, comprising tributyl phosphate (TBP) as a hydrogen bond acceptor and acetic acid (AcOH) as a hydrogen bond donor. The total antioxidant capacity (TAC) of tomato and carrot extracts obtained by MAE, in which optimization of operational parameters and modeling were made with the use of Box-Behnken design of the response surface methodology (RSM), was evaluated using the Cupric Reducing Antioxidant Capacity (CUPRAC) method. For the highest TAC, operational parameters that best suit the MAE procedure were set at 80 °C, 35 min, and 25 mL/2.0 g. The TAC values of extracts obtained by MAE using TBP:AcOH, 1:2 (mol/mol) were examined against those of extracts acquired by classical solvent extraction using a mixture of hexane, ethanol and acetone (H:E:A, 2:1:1 (v/v/v)) mixture. TAC of extracts in DES varied between 5.10 and 0.71 lycopene equivalents (mmol LYC kg−1). The highest extraction yield comparable to conventional organic solvents was obtained with TBP:AcOH (1:2). It was observed that, in addition to lipophilic antioxidants, some hydrophilic antioxidant compounds were partially extracted with the proposed DES. Moreover, the extracted antioxidant compounds were identified and quantified by HPLC analysis. The proposed DES and MAE process will find potential application for hydrophobic antioxidant extraction from tomatoes and carrots on an industrial scale after further studies.

Sustainable Bio-Dyeing of Cellulosic-Based Fabrics with Anthocyanins from Black Carrot (Daucus carota L.)

Abstract In this study, anthocyanin-based black carrot (Daucus carota L.) extract was investigated as a natural textile dye on cellulose-based flax and cotton. Sodium alginate and gall oak (Quercus infectoria Olivier) were used as bio-mordants. The optimal dyeing conditions were determined by varying the amounts of these organic bio-mordants. A CIEL*a*b* spectrophotometer was used to measure the color characteristics of the dyed samples. The RP-HPLC-DAD method was used to identify the dye components in all dyed fabrics. Moreover, the dyed fabrics were evaluated for color fastness to washing, light, and rubbing. The mixture of these bio-mordants gave good color efficiency and acceptable colorfastness test results. Consequently, it appeared that black carrot (Daucus carota L.) can be used to color natural fibers. Applying these natural dye sources and bio-mordants may easily produce various colors.

The Quality Analysis of Of Rolled Pancake with Carrot Extract Substitution as Natural Food Colouring

This research aims to analyze the effect of 0%, 15%, 30% and 45% substitution of carrot extract to the quality of rolled pancakes in terms of shape, color, aroma, texture and taste. This research was conducted in August 2023 at the Culinary Workshop, Department of Family Welfare Sciences, Faculty of Tourism and Hospitality, Padang State University. This type of research is a pure experiment using a completely randomized design method consisting of 4 treatments and 3 repetitions. The data used is primary data obtained from 3 expert panelists using an organoleptic test format on the quality of rolled pancakes with the substitution of carrot extract. The data obtained was then tabulated in table form and analysis of variance (ANOVA) was carried out. If F count &gt; F table then proceed with the Duncan test. The results of this research show that there is a significant effect of substitution of 0%, 15%, 30% and 45% carrot extract on the quality of color and aroma of rolled pancakes. The results of data analysis obtained the highest overall achievement scores, namely: neat shape 3.56 (45%), uniform shape 3.44 (30%), color 3.44 (45%), aroma 3.67 (45%), texture 3.67 (45%) and taste 4.00 (45%). The results of the quality test for rolled pancakes with the best carrot extract substitution were 45% (X3).

Peanut (Arachis hypogaea) Milk Vegan Cheese with Pineapple (Ananas comosus) Bromelin Enzyme as Coagulant and Carrot (Daucus carota) Extract as Source of Antioxidants

Peanut (Arachis hypogaea) Milk Vegan Cheese with Pineapple (Ananas comosus) Bromelin Enzyme as Coagulant and Carrot (Daucus carota) Extract as Source of Antioxidants

Extraction and food enrichment applications of black carrot phytocompounds: A review

Black carrots are an essential component of the human diet due to the presence of various beneficial nutrients, including polyphenols, anthocyanins, and phenolic acids. Black carrots can be added to enhance the color, taste, and nutritional value of a number of food products. Black carrots provide several health advantages, including antioxidant, anti-inflammatory, and anticancer characteristics. Black carrots lose some of their antioxidant power during extraction and during the storage period. The most prevalent anthocyanin in black carrot is cyanidin-3-xylosyl-feruloyl-glucosyl-galactoside. It is critical to consider the stability of anthocyanins while working with black carrot extracts. Anthocyanin stability may alter in response to environmental conditions like heat, light, pH, and oxygen. Anthocyanins may undergo photochemical breakdown when exposed to light, and thermal breakdown when exposed to high temperatures. The pH may also affect the stability of anthocyanins, with a low pH leading to colorless and reduced stability. This review discusses the storage stability of bioactive components in black carrots. This study discusses the various polyphenols present in black carrots and their potential advantages, including antioxidant, anti-inflammatory, anticancer, antidiabetic, and anti-hypersensitivity actions. The numerous pharmacological functions of the flavonoids found in black carrots, including quercetin, luteolin, and myricetin are discussed.

Wounding stress enhances the anti-obesogenic, anti-inflammatory, and antioxidant properties of carrots (Daucuscarota)

This study evaluated the health benefits of carrots (Daucus carota) subjected to post-harvest wounding stress, emphasizing their anti-obesogenic, anti-inflammatory, and antioxidant properties. Carrots were shredded and stored at 15 ± 2 °C for 48 h to induce wounding stress, leading to a significant increase in chlorogenic acid content, by over 112.5 % compared to control. The study utilized 3T3-L1 cell differentiation assays to evaluate the anti-obesogenic potential, monitoring changes in lipid accumulation and adipogenesis-related gene expression. The anti-inflammatory activity was determined in LPS-stimulated RAW 264.7 macrophages by measuring nitrite production, while the antioxidant capacity was assessed using the DCFH-DA assay in Caco-2 cells. Key findings include a notable reduction in lipid accumulation in 3T3-L1 cells with stressed carrot extracts, particularly at 166 μg/mL concentration, showing an 18 % decrease compared to the control. Furthermore, there was a significant alteration in the expression of adipogenesis-related genes, with a 73 % decrease in FAS expression and a 160 % increase in ATGL expression. The stressed carrot extracts at 125 μg/mL also exhibited enhanced anti-inflammatory activity, inhibiting nitrite production by 44.60 %, a 153.26 % increase over the lower concentration. Additionally, antioxidant activity in stressed carrot extracts increased by 77.15 % at 125 μg/mL. These results indicate that wounding stress significantly boosts the health-promoting properties of carrots, suggesting their potential as functional foods for combating obesity and metabolic syndrome-related diseases. The study highlights the value of post-harvest treatments in enhancing the nutraceutical quality of vegetables.

Application of On-Pack pH Indicators to Monitor Freshness of Modified Atmospheric Packaged Raw Beef

Abstract Three on-pack visual indicators for the real-time monitoring of raw beef steaks in a modified atmospheric packaging (MAP) environment were developed. The indicators were prepared by spray deposition of a nanocellulose (NC) suspension (1.5 wt. %) with the desired concentration of the pH-sensitive indicators, either red cabbage (RC) extract, black carrot (BC) extract, or chlorophenol red (CPR). The responsiveness of the colorimetric pH indicators, assessed visually and by CIE-Lab quantitative analysis, to the freshness of raw beef steaks stored under MAP conditions at 4 °C or 20 °C, was analysed over 7 days. All indicators gave a colour change for beef steak stored at 4 °C for 7 days that was noticeable with the naked eye and had a ΔE value &amp;gt;12. The sensitivity of the RC pH indicator was superior to the BC and CPR pH indicators. A study linking total microbial count (aerobic + Escherichia coli + coliform) and the quantitative colorimetric response of the indicators (ΔE) revealed a strong linear correlation. Thus, it was concluded that the developed colorimetric pH indicators could be used to monitor the freshness of raw beef and as an alternative to the best-before date commonly used in pre-packaged meat.

Using Black Carrot Extracts as an Alternative Biological Dye for Tissue Staining

Background: Tissue staining is pivotal in histology and histopathology, shouldering a noteworthy role in identifying and classifying tissues and diseases. Due to their non-production of toxic effluents, the utilization of plant-based dyes aligns harmoniously with environmental sustainability and the well-being of laboratory personnel and the general public. Furthermore, this approach is highly cost-effective, further enhancing its appeal. Objectives: This research study explored the feasibility of staining various tissues in mice, such as the liver, kidney, intestine, and cartilage, utilizing a dye extracted from black carrots. Methods: An ethanol extract of 200 g of fresh black carrots (Daucus carota L.) was prepared using 95% ethanol saturated with two different solvents in 200 mL of distilled water. Subsequently, the prepared sections of mice tissue were immersed in the extracted dye solution for 20 minutes, followed by assessment using a light microscope. Hematoxylin-eosin staining was used as a control. Results: The dye extracted from the black carrot using alum and acetic acid successfully stained the cartilage, kidney, intestine, and liver tissues, giving them a bluish-gray coloration. Phytochemical screening further confirmed the presence of anthocyanins in the black carrot extract. Conclusion: The dye derived from black carrots exhibits natural tissue staining capabilities, making it an alternative to hematoxylin-eosin in histology and histopathology laboratories.

Optimizing encapsulation of black carrot extract using complex coacervation technique: Maximizing the bioaccessibility and release kinetics in different food matrixes

The encapsulation of bioactive compounds at the micro-scale presents an advanced approach for the food and nutraceutical industries. However, the challenge lies in determining optimal encapsulation parameters for each specific bioactive compound and encapsulation method. In addressing this, our study demonstrates the application of statistical programs to streamline the optimization of wet-lab experimental designs. Focusing on the encapsulation technique of complex coacervates for black carrot phenolic extract (BCPE), the response surface methodology was employed to optimize encapsulation parameters in terms of coating material, the core-to-coating material ratio, and the pH of the encapsulation environment. The optimum conditions predicted by RSM to produce BCPE-loaded complex coacervates were found to be a pH of 3.02, a core-to-coating ratio of 10g/100g, and a coating material composition of 59.10g/100 mL maltodextrin, and 0.90g/100 mL whey protein isolate. According to the RSM pattern with 84% desirability, encapsulation efficiency was found 86.08%. In addition, the effect of different food matrixes was examined on the in vitro bioaccessibility of spray-dried BCPE loaded complex coacervated powder (BCPE-CCp). To explore the impact of protein and carbohydrate richness, along with food temperature on capsule stability, the BCPE-CCp was incorporated into skim milk, apple juice, and chocolate beverages (1g/100 mL). Notably, heat treatment had no significant effect on the in vitro bioaccessibility of BCPE-CCp in terms of total phenolic compound and antioxidant activity (p < 0.05), indicating its suitability for hot formulations. Furthermore, the release of BCPE in a protein-rich environment was observed to be higher than in a carbohydrate-rich food matrix under both gastric and intestinal conditions. These findings provide valuable insights into the stability and release dynamics of BCPE-CCp in different food settings, supporting its adaptability for diverse formulations, including those involving elevated temperatures.

The study of the physicochemical properties of rectal cream samples with the carrot extract and rutin

The modern arsenal of drugs aimed at eliminating pathological processes of the anorectal zone, as a rule, consists of suppositories and soft drugs. When using suppositories, hemorrhoidal nodes may be injured, uneven distribution of the drug along the walls of the anal canal may be observed. In addition, with external and combined hemorrhoids, there is a need to apply the drug not only to the walls of the anal canal, but also to the perianal skin. Therefore, preference is given to the use of soft drugs since the process of their introduction is as easy as possible. Today, the pharmaceutical market of Ukraine has a wide range of products for the treatment of diseases of the anorectal zone, but the constant increase in the number of patients indicates that this problem has not been solved. Therefore, the study on the creation of a new rectal soft medicine that will have a complex effect on pathological processes and expand the range of domestic drugs for use in proctology is relevant. Aim. To substantiate the type of base for a soft rectal medicine by studying the organoleptic and physicochemical properties of the experimental samples. Materials and methods. The study objects were samples of a soft dosage form of various compositions and the reference drug “Procto-Glivenol Cream” (VAMPHARMA S.R.L., Italy). Samples on different carrier bases were examined by their organoleptic and textural properties; their colloidal and thermal stability, viscosity and pH were studied. Results and discussion. It has been found that the use of a sample on a gel base with Aristoflex does not ensure the presence of an optimal adhesive layer between the mucous membrane of the anorectal zone and the dispersion medium of the drug. The proxanol-188 sample does not have satisfactory thermal and colloidal stability indicators. The use of an emulsion base provides the necessary ability to spread over the surface, adhesion and cohesion. Conclusions. Therefore, for further research, it is advisable to use a sample of the rectal cream, which is an emulsion system.

Comparison of Physical and Chemical Analyzes, Antioxidant Activity, Anthocyanin Amounts of Tarhana with Black Carrot Extract and Classical Tarhana

Tarhana, which is very rich in nutritional value, has been the crown of our tables for centuries and is one of our common values wherever we live. It is obtained with black carrot extract to give this special food an even richer form. The effects of foods rich in anthocyanins on health (such as anti-inflammatory, anti-diabetic, anti-tumor, anti-ulcer, antioxidant, anti-carcinogenic) are known. Black carrot extract was used to increase the anthocyanin content of the widely consumed tarhana. In this study, physical and chemical analyzes, total phenolic content and total flavonoid content, antioxidant activities, colour measurement, sensory analysis of tarhana prepared with black carrot extract were determined. At the same time, the total amount of monomeric anthocyanin was determined. All these values were compared with classical tarhana and it was determined that tarhana containing black carrot extract had high DPPH activity (4.21±1.78 mg/mL) and high anthocyanin content (19.14±2.02 mg cyn3-glu/kg sample). According to the sensory analysis, the acceptability of tarhana with black carrot extract was determined to be high.

VITAMIN A CONTENT AND HEDONIC TEST OF MODIFIED MODISCO SLURP PUDDING WITH VARIOUS CARROT EXTRACT ADDITIONS

Backgorund : A Modisco is a form of PMT-P as a treatment for malnutrition and severe malnutrition. To increase the acceptance and function of nutritional sources, Modisco is done by modifying its shape and composition to become Modisco slurp pudding with the addition of carrot juice.&#x0D; Objectives : The purpose of this study was to analyze the content of Vitamin A and the results of the favorite test of the modisco suction pudding with the addition of carrot juice variations of 0%, 5%, 10%, and 15%.&#x0D; Method : This study used an experimental research method in the form of a Posttest Only Control Group Design design. The design of this study used two groups, namely the treated group, namely the modisco suction pudding with the addition of various carrot extracts and the untreated group, the modisco suction pudding without the addition of carrot juice.&#x0D; Results : The results of the statistical test of Vitamin A levels using One-Way ANOVA showed a significance value of 0.0001 (&lt;0.05) or there was a significant difference in Vitamin A levels in the modisco suction pudding with variations in the addition of carrot juice.&#x0D; Conclusion : The statistical results of the preference test show that only the aroma and taste are significantly different, while the color and texture are not significant. Sample X0 has a preferred aroma and taste compared to X1, X2, and X3. Based on the Vitamin A content test and the Preferred Test X1, the value of Vitamin A content was closest to the nutritional requirement of toddlers for Vitamin A and was the most preferred treatment group.&#x0D;