Herbivores Research Articles

Comparative transcriptomics of Venus flytrap (Dionaea muscipula) across stages of prey capture and digestion.

The Venus flytrap, Dionaea muscipula, is perhaps the world's best-known botanical carnivore. The act of prey capture and digestion along with its rapidly closing, charismatic traps make this species a compelling model for studying the evolution and fundamental biology of carnivorous plants. There is a growing body of research on the genome, transcriptome, and digestome of Dionaea muscipula, but surprisingly limited information on changes in trap transcript abundance over time since feeding. Here we present the results of a comparative transcriptomics project exploring the transcriptomic changes across seven timepoints in a 72-hour time series of prey digestion and three timepoints directly comparing triggered traps with and without prey items. We document a dynamic response to prey capture including changes in abundance of transcripts with Gene Ontology (GO) annotations related to digestion and nutrient uptake. Comparisons of traps with and without prey documented 174 significantly differentially expressed genes at 1 hour after triggering and 151 genes with significantly different abundances at 24 hours. Approximately 50% of annotated protein-coding genes in Venus flytrap genome exhibit change (10041 of 21135) in transcript abundance following prey capture. Whereas peak abundance for most of these genes was observed within 3 hours, an expression cluster of 3009 genes exhibited continuously increasing abundance over the 72-hour sampling period, and transcript for these genes with GO annotation terms including both catabolism and nutrient transport may continue to accumulate beyond 72 hours.

Strawberry growth and dry matter partitioning due to fertigation systems

Strawberry cultivation possesses unique features, including the need for high-quality seedlings adapted to the cultivation environment and proper fertigation management. These elements influence plant growth and production. This study evaluated the growth and dry matter partitioning of strawberry plants of the cultivar "Albion" at different seedling ages (0, 14, 28... 196 DAT) using two fertigation methods: open-loop and closed-loop systems. Fifteen evaluations occurred throughout the cultivation cycle, spanning from planting to 196 days after transplanting (DAT), with the study replicated over two consecutive years. We evaluated dry matter partitioning among roots, stems, leaves, senescent leaves, flowers, commercial fruits, and non-commercial fruits. We also measured root volume and length, besides counts of leaves, flowers, and both commercial and non-commercial fruits. Despite no significant differences between fertigation systems for most variables, the closed-loop system shows promise, contingent on proper nutrient solution management. The partitioning analysis revealed that fruits, followed by leaves, were the primary consumers of photoassimilates.

Micropropagation and genetic transformation of Byblis liniflora

AbstractByblis, a small genus of carnivorous plants predominantly found in Australia, is characterized by its passive trapping mechanism and unique floral features. The chemical composition of Byblis, including identified phenylethanoid glycosides, particularly acteoside, highlights its pharmacological potential with various biological activities. In vitro culture techniques have been established for propagation, with micropropagation protocols developed for different Byblis species. However, information on genetic transformation, vital for trait modification and enhanced pharmacological interest, remains limited. This study focuses on optimizing micropropagation, adventitious regeneration, and genetic transformation methods for Byblis liniflora. Adventitious regeneration rates were highest in medium with reduced Murashige and Skoog salts (MS/10) and sucrose (3 gL−1) concentrations. Zeatin supplementation (1 mgL−1) further improved regeneration rates and bud development with 100% of regenerated root explants and 8.8 shoots per explant. Liquid MB3 medium supplemented with indole-3-acetic acid (IAA) 5 mgL−1 facilitated efficient rooting and acclimatization. The establishment of an efficient Rhizobium-mediated genetic transformation method yielded transgenic plants expressing green fluorescent protein (GFP). Molecular analysis confirmed transgene integration, marking the first successful genetic transformation in the Byblis genus. These advancements pave the way for exploring gene function and enhancing pharmacological properties, thereby broadening our understanding and utilization of carnivorous plants like Byblis.

Growth and biomass of Wolffia arrhiza with different sources of organic fertilizer

Wolffia arrhiza has a growth rate and biomass as well as a good nutritional content for the growth of cultivated fish, especially herbivorous fish. Due to the high protein content (up to 45% of the dry weight), Wolffia is an excellent plant for the production of various biologically active substances, especially proteins. These plants can double every 1-6 days, similar to algae or mushrooms, and the number of leaves doubles every 2-3 days. This study aims to determine the effect of different fertilizer sources on doubling time, relative growth rate, population density and biomass of Wolffia arrhiza and water quality. The method used in this research is experimental. The research design used a completely randomized design (CRD) consisting of 4 treatments and 3 replications. The treatments were as follows: treatment A: chicken manure, B: cow manure, C: buffalo manure and D: goat manure with each dose of 200 grams. Parameters observed included doubling time, relative growth rate, population density, biomass and water quality parameters. The results showed that treatment B (cow manure) gave the highest value for doubling time, 1.59 days, relative growth rate 0.392 g/day, population density 209.75 ind/cm2, biomass 94.97. Keyword: Biomass; Fertilizer; Growth; Wolffia

To eat or not to eat: novel stable isotope models reveal a shift in carnivory with nutrient availability for aquatic Utricularia spp.

Freshwater nitrogen inputs are increasing globally, altering the structure and function of wetland ecosystems adapted to low nutrient conditions. Carnivorous wetland plants of the genus Utricularia are hypothesized to reduce their reliance on carnivory and increase their assimilation of environmental nutrients when the supply of ambient nutrients increases. Despite success in using stable isotope approaches to quantify carnivory of terrestrial carnivorous plants, quantifying carnivory of aquatic Utricularia requires improvement. We developed stable isotope mixing models to quantify aquatic plant carnivory and used these models to measure dietary changes of three Utricularia species, Utricularia australis, U. gibba and U. uliginosa, in 11 wetlands across a 794-km gradient in eastern Australia. Diet was assessed using multiple models that compared variations in the natural-abundance nitrogen isotope composition (δ15N) of Utricularia spp. with that of non-carnivorous plants, and environmental and carnivorous nitrogen sources. Carnivory supplied 40-100 % of plant nitrogen. The lowest carnivory rates coincided with the highest availability of ammonium and dissolved organic carbon. Our findings suggest that Utricularia populations may adapt to high nutrient environments by shifting away from energetically costly carnivory. This has implications for species conservation as anthropogenic impacts continue to affect global wetland ecosystems.

Graph-theoretic modeling reveals connectivity hotspots for herbivorous reef fishes in a restored tropical island system

ContextSeascape connectivity refers to how the spatial configuration of marine habitats facilitates or hinders the movement of organisms, nutrients, materials or energy. Predicting and ranking potential connectivity among habitat patches for coral reef fishes helps to understand how reef fishes could utilize and connect multiple habitat types through the flow of nutrients, energy and biomass across the wider seascape during foraging movements.ObjectivesTo advance a spatially explicit understanding of connectivity linkages within a tropical atoll system by modeling, mapping and quantifying potential seascape connectivity for two locally abundant herbivorous reef fish species, the parrotfish, Chlorurus spilurus (pahoro hohoni or pa’ati pa’apa’a auahi), and the surgeonfish, Acanthurus triostegus (manini).MethodsWe applied a two-step modeling approach by first mapping habitat suitability for the focal species. A graph-theoretic modeling technique was then applied to model and measure the contribution of benthic habitat patches to species-specific potential connectivity within the seascape.ResultsHabitat suitability was higher and less fragmented for C. spilurus than for A. triostegus. Potential ecological connectivity estimates for C. spilurus were higher across the entire seascape, with differences between species likely driven by local-scale benthic habitat patch configuration and species home ranges. Hotspots of ecological connectivity across the atoll were mapped for both species.ConclusionsDespite advances in the application of graph-theoretic techniques in the coastal environment, few marine conservation and restoration measures currently integrate spatial information on ecological connectivity. This two-step spatial modeling approach holds great potential for rapid application of connectivity modeling at multiple spatial scales, which may predict ecological responses to conservation actions including active habitat restoration.

Range-wide salamander densities reveal a key component of terrestrial vertebrate biomass in eastern North American forests.

Characterizing the population density of species is a central interest in ecology. Eastern North America is the global hotspot for biodiversity of plethodontid salamanders, an inconspicuous component of terrestrial vertebrate communities, and among the most widespread is the eastern red-backed salamander, Plethodon cinereus. Previous work suggests population densities are high with significant geographic variation, but comparisons among locations are challenged by lack of standardization of methods and failure to accommodate imperfect detection. We present results from a large-scale research network that accounts for detection uncertainty using systematic survey protocols and robust statistical models. We analysed mark-recapture data from 18 study areas across much of the species range. Estimated salamander densities ranged from 1950 to 34 300 salamanders ha-1, with a median of 9965 salamanders ha-1. We compared these results to previous estimates for P. cinereus and other abundant terrestrial vertebrates. We demonstrate that overall the biomass of P. cinereus, a secondary consumer, is of similar or greater magnitude to widespread primary consumers such as white-tailed deer (Odocoileus virginianus) and Peromyscus mice, and two to three orders of magnitude greater than common secondary consumer species. Our results add empirical evidence that P. cinereus, and amphibians in general, are an outsized component of terrestrial vertebrate communities in temperate ecosystems.

Determinants of Firm Value: Empirical Evidence from The Primary Consumer Goods Sector Companies

Investor decisions to invest in a company are partially influenced by the company's value. Investors are increasingly attracted to companies that can generate sustainable profits, ultimately enhancing the company's value. This study aims to analyze the influence of intellectual capital, profitability, leverage, and asset turnover on company value, using a sample of companies listed on the Indonesia Stock Exchange in the primary consumer products sector between 2017 and 2021. Using purposive sampling, 61 companies were selected as samples. With observations over 5 years, a total of 305 data observations were obtained. Multiple linear regression analysis was applied to secondary data sourced from the financial accounts of the selected organizations. The correlational relationship between the dependent variable and the regressors was determined by analyzing the data obtained using SPSS tools. The study results indicate that intellectual capital does not affect company value, profitability, and asset turnover positively affect company value, but leverage negatively affects company value. The positive impact of profitability and asset turnover indicates that an increase in these factors will enhance the company's value. Conversely, the negative impact of leverage suggests that an increase in leverage will decrease the company's value.

Evolutionary emergence of plant and pollinator polymorphisms in consumer-resource mutualisms

Mutualism is considered a major driver of biodiversity, as it enables extensive codiversification in terrestrial communities. An important case is flowering plants and their pollinators, where convergent selection on plant and pollinator traits is combined with divergent selection to minimize niche overlap within each group. In this article, we study the emergence of polymorphisms in communities structured trophically: plants are the primary producers of resources required by the primary consumers, the servicing pollinators. We model natural selection on traits affecting mutualism between plants and pollinators and competition within these two trophic levels. We show that phenotypic diversification is favored by broad plant niches, suggesting that bottom-up trophic control leads to codiversification. Mutualistic generalism, i.e., tolerance to differences in plant and pollinator traits, promotes a cascade of evolutionary branching favored by bottom-up plant competition dependent on similarity and top-down mutualistic services that broaden plant niches. Our results predict a strong positive correlation between the diversity of plant and pollinator phenotypes, which previous work has partially attributed to the trophic dependence of pollinators on plants.

Energy efficiency in medium-scale airports: Two Mexican airports as case of study

Reducing energy consumption in the operation of airports has been identified as one of the approaches to achieve the commitments of the countries in reducing their greenhouse gas (GHG) emissions. The first step in this approach is the development of an energy diagnostic. However, multiple practical aspects remain unresolved when applying the existing methodologies to perform energy diagnostics, especially in the case of small and medium-scale airports. Seeking to address these issues, this work presents energy diagnostics of two Mexican international airports so that it can be used to carry out energy diagnostics in other airports with similar characteristics. Emphasis is given to identifying and prioritizing, from a sustainable point of view, the strategies to reduce energy consumption and GHG emissions. The Ciudad del Carmen Airport (CME) is located in a nearshore region with high ambient temperatures (27 °C) and humidities. It was found that in 2019, the CME airport consumed 123 MWh with an average of 577 Wh per passenger, with the HVAC system being the primary energy consumer. Critical strategies for the CME airport include photovoltaic systems and HVAC renovation. In contrast, the Puebla airport (PBC) is located in a region with comfortable ambient conditions (16 °C). In 2019, the PBC airport consumed 61.31 MWh/year and 442 Wh per passenger. The main strategies for PBC include expanding its photovoltaic energy generation system, employee awareness programs, and renewing the vehicle fleet with electric vehicles.

Effects of Aeromonas infection on the immune system, physical barriers and microflora structure in the intestine of juvenile grass carp (Ctenopharyngodon idella)

Grass carp (Ctenopharyngodon idella) is an intensively cultured and economically important herbivorous fish species in China, but its culture is often impacted by Aeromonas pathogens such as Aeromonas hydrophila and Aeromonas veronii. In this study, healthy grass carp were separately infected with A. hydrophila or A. veronii for 12, 24, 48 or 72 h. The results showed that the mRNA expression levels of intestinal inflammatory factors (tnf-α, il-1β and il-8), complement factors (c3 and c4), antimicrobial peptides (hepcidin, nk-lysin and β-defensin-1), immunoglobulins (igm and igt), and immune pathway-related signaling molecules (tlr1, tlr2, tlr4, myd88, irak4, irak1, traf6, nf-κb p65 and ap-1) were differentially upregulated in response to A. hydrophila and A. veronii challenge. Additionally, the expression levels of the intestinal pro-apoptotic genes tnfr1, tnfr2, tradd, caspase-8, caspase-3 and bax were significantly increased, whereas the expression of the inhibitory factor bcl-2 was significantly downregulated, indicating that Aeromonas infection significantly induced apoptosis in the intestine of grass carp. Moreover, the expression of intestinal tight junction proteins (occludin, zo-1, claudin b and claudin c) was significantly decreased after infection with Aeromonas. Histopathological analysis indicated the Aeromonas challenge caused severe damage to the intestinal villi with adhesions and detachment of intestinal villi accompanied by severe inflammatory cell infiltration at 12 h and 72 h. The 16S rRNA sequencing results showed that Aeromonas infection significantly altered the structure of the intestinal microflora of the grass carp at the phylum (Proteobacteria, Fusobacteria, Bacteroidetes and Firmicutes) and genus (Proteus, Cetobacterium, Bacteroides, and Aeromonas) levels. Take together, the findings of this study revealed that Aeromonas infection induces an intestinal immune response, triggers cell apoptosis, destroys physical barriers and alters microflora structure in the intestine of juvenile grass carp; the results will help to reveal the pathogenesis of intestinal bacterial diseases in grass carp.

Bayesian Meta-Analysis: Impacts of Eating Habits and Habitats on Omega-3 Long-Chain Polyunsaturated Fatty Acid Composition and Growth in Cultured Fish.

Omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFAs) such as eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) offer protective benefits against various pathological conditions, including atherosclerosis, obesity, inflammation, and autoimmune diseases. Marine fish and seafood are the primary sources of n-3 LC-PUFAs in the human diet. However, the inclusion of fish oil in aquafeeds is declining due to limited availability, fluctuating prices, sustainability concerns, and replacement with vegetable oils. While comprehensive narrative reviews on the impact of substituting fish oil with vegetable oil in aquafeeds exist, quantitative studies are relatively scarce and mainly focused on comparing the source of vegetable oils. Herein, we employed, for the first time, a Bayesian meta-analysis approach, collecting research data from 81 articles to quantitatively analyze the effects of dietary n-3 LC-PUFA levels on the n-3 LC-PUFA composition and growth performance in cultured fish. Our findings indicate that with the exception of herbivorous fish, dietary n-3 LC-PUFA levels significantly affect the EPA and DHA levels in the livers and muscles of carnivorous, omnivorous, freshwater, and marine fish. Additionally, the growths of freshwater and herbivorous fish were less affected by changes in dietary n-3 LC-PUFA levels compared to that of carnivorous and marine fish.

Peak patterns and drivers of city-level daily CO2 emissions in China

Cities are the primary carbon emitters in China and play a critical role in achieving national and regional emission-reduction targets. However, because existing CO2 emission datasets are mostly limited to national and provincial levels and have significant time lags, there are few studies on urban emission patterns and drivers below the annual level. In this study, utilizing near-real-time daily CO2 emission data from Carbon Monitor Cities-China, we analyzed the seasonality and peak patterns of city-level emissions and conducted a decomposition of emission drivers by sector based on sociometric and natural factors. Our results indicated that most cities in China exhibited seasonal emissions, with peak emissions occurring during winter. However, heavily industrialized cities tend to produce high year-round emissions. The residential and power sectors are crucial for reducing emissions in winter-peaked cities, whereas the industrial and power sectors are the primary energy consumers in non-winter-peaked cities. The results of the decomposition analysis show that the emission structure of the service industry and the impact of temperature on power generation are the primary drivers of CO2 emissions during winter. The above analysis offers recommendations for developing emission-reduction policies for various types of cities. Service-based cities should concentrate their efforts on reducing emissions from tertiary industries, whereas energy-dependent cities should continue to focus on controlling emissions from secondary industries such as industry and power. In addition, both service-based and energy-dependent cities must address the significant impact of heating demands on emissions.

GONAD MATURITY LEVELS OF LEMPUK FISH (Gobiopterus sp) IN RANU GRATI, PASURUAN, EAST JAVA

Pasuruan Regency in East Java Province boasts a natural lake, Ranu Grati, formed by volcanic activity, covering about 50 hectares and home to endemic species like the Lempuk fish (Gobiopterus sp.). These fish play a crucial role in Ranu Grati's trophic structure, serving as primary consumers and key planktonic organisms supporting higher trophic levels. The Lempuk fish population faces sustainability challenges due to high demand for this traditional food, leading to overexploitation and decreased reproductive capabilities. Understanding the reproductive biology, particularly gonad maturity levels, is vital for effective resource management. This study, conducted from June to August 2023, aimed to investigate the reproductive biology and gonad maturity levels of Lempuk fish in Ranu Grati. Analysis revealed a male-biased sex ratio, with significant fluctuations in gonad maturity levels across months. In June, the sex ratio was 1.05, in July 1.17, and in August 1.39, indicating a higher population of males. Histological observations showed asynchronous egg development, likely influenced by environmental factors and the fish's reproductive strategy

Beyond biomass: Resource effects on primary production and consumer nutrient assimilation in streams

Abstract Primary production in aquatic ecosystems is strongly controlled by resource availability (bottom‐up). At the same time, grazers exert top‐down pressure on algae. The effects of both resources and grazers on algal communities have been investigated extensively for both benthic and planktonic systems. However, most studies focus exclusively on net effects on algal standing stock, while the underlying ecological functions and processes often remain unknown. Here, we tested the effects of the two resources, light (L) and the limiting nutrient phosphorus (P), on both primary production (net oxygen production) by benthic algae (periphyton) and matter assimilation by the next trophic guild (particularly nitrogen assimilation by grazers). For this, we grew natural periphyton communities in stream mesocosm flumes together with two native grazer taxa and 15N as a tracer. Both resources resulted in an initial increase in net primary production, but this effect equalised after 36 days in the more mature communities. While both resources still showed strong positive effects on algal biovolume, the biovolume‐specific net primary production decreased with resource level and was lowest in the double elevated (+L, +P) treatment. The response of nitrogen assimilation by grazers was more closely related to the response of periphyton quality (stoichiometric and taxonomic composition) than to its biomass. In addition, light affected the nitrogen assimilation of both grazers negatively. These findings demonstrate that the responses of ecological functions, here primary production and assimilation by grazers, are not necessarily proportional to algal biomass and are thus not easily predictable from the common use of biomass as a proxy for functions.

Processes regulating local community assembly of plants and spiders in high latitude mountains

AbstractAimMountains are often used to study how environmental factors influence biodiversity. However, we have limited understanding of the processes causing biodiversity variation in mountains and whether such processes vary across trophic levels and spatial scales. The aim of this study was to evaluate (i) whether community assembly processes varied along elevational gradients, (ii) whether there were differences in such variation between primary producers (vascular plants) and secondary consumers (spiders) and (iii) whether there were scale dependencies in any elevational variation in community assembly.LocationFennoscandia, Northern Sweden.TaxonVascular plants, spiders.MethodsWe used phenotypic and phylogenetic dispersion to quantify how elevation influenced community assembly of vascular plants and spiders and whether there were any scale dependencies in such influences. Our original data of plant and spider communities came from our own field surveys, phenotypic dispersion was calculated based on matrices of ecological traits, and phylogenetic dispersion was calculated from phylogenetic trees for each organism group. Trait matrices were based on a combination of literature values and our own measurements. The phylogeny for vascular plants was based on a published plant super‐tree, whereas the phylogeny for spiders was created by ourselves based on the DNA sequences at the mitochondrial cytochrome c oxidase subunit 1 (COI).ResultsPlants were environmentally filtered throughout all elevations and scales, but the importance of convergent evolution increased with elevation. For spiders, the importance of environmental filtering as well as niche conservatism increased with elevation. For both groups, communities at smaller scales were more influenced by biotic regulation and niche conservatism than at larger scales.Main ConclusionsOur study highlights both taxonomic differences and scale dependencies in how elevation influences community assembly. We argue that these results can have broad ramifications for our understanding of how spatial variation in biodiversity is generated and maintained. This may have particular relevance for our ability to predict the ecological consequences of climate change. Our results further highlight that high elevation specialists may suffer increased risks of climate driven extinctions due to a combination of increased competition and increased fragmentation of suitable habitats. Particularly for spiders, which had high elevation specialists clustered along specific lineages, such extinctions could lead to significant loss of phylogenetic variation.

The smallest angiosperm genomes may be the price for effective traps of bladderworts.

Species of the carnivorous family Lentibulariaceae exhibit the smallest genomes in flowering plants. We explored the hypothesis that their minute genomes result from the unique mitochondrial cytochrome c oxidase (COX) mutation. The mutation may boost mitochondrial efficiency, which is especially useful for suction-bladder traps of Utricularia, but also increase DNA-damaging reactive oxygen species, leading to genome shrinkage through deletion-biased DNA repair. We aimed to explore this mutation's impact on genome size, providing insights into genetic mutation roles in plant genome evolution under environmental pressures. We compiled and measured genome and mean chromosome sizes for 127 and 67 species, respectively, representing all three genera (Genlisea, Pinguicula, and Utricularia) of Lentibulariaceae. We also isolated and analyzed COX sequences to detect the mutation. Through phylogenetic regressions and Ornstein-Uhlenbeck models of trait evolution, we assessed the impact of the COX mutation on the genome and chromosome sizes across the family. Our findings reveal significant correlations between the COX mutations and smaller genome and chromosome sizes. Specifically, species carrying the ancestral COX sequence exhibited larger genomes and chromosomes than those with the mutation. This evidence supports the notion that the COX mutation contributes to genome downsizing, with statistical analyses confirming a directional evolution towards smaller genomes in species harboring these mutations. Our study confirms that the COX mutation in Lentibulariaceae is associated with genome downsizing, likely driven by increased reactive oxygen species production and subsequent DNA damage requiring deletion-biased repair mechanisms. While boosting mitochondrial energy output, this genetic mutation compromises genome integrity and may potentially affect recombination rates, illustrating a complex trade-off between evolutionary advantages and disadvantages. Our results highlight the intricate processes by which genetic mutations and environmental pressures shape genome size evolution in carnivorous plants.

Tobin's Q dan Determinannya: Studi Empiris

This research aims to analyze factors that influence firm value, including profitability, leverage, liquidity and firm size. Research on 56 Primary Consumer Goods Sector Companies in Indonesia with an observation period of 2017 to 2021. Data analysis carried out using multiple linear regression shows that profitability has a positive effect while leverage has a negative effect on firm value. This research fails to prove the role of liquidity and company size in explaining changes in firm value.

Food odors elicit feeding behavior through the activation of the olfactory system in blunt snout bream (Megalobrama amblycephala)

Olfaction plays an important role in the survival and reproduction of various organisms. Aside from being a nutrient, in many fish species, amino acids also function as attractants to induce feeding behaviors. As the herbivorous fish, blunt snout bream (Megalobrama amblycephala) commonly consumes an aquatic plant, Hydrilla verticillata. Here, we studied the effect of food odors on the feeding behavior of blunt snout bream using a mixture of amino acids and H. verticillata extract. The behavioral and electro-olfactogram tests demonstrated that the mixture of amino acids and H. verticillata extract induced an attractive reaction and olfactory response in blunt snout bream, respectively. Through Western blotting and immunohistochemical analyses, we confirmed that ciliated and microvillus neurons are distributed on the olfactory placode. The results of phospho-extracellular regulated protein kinases (pERK) showed that a large number of microvillus and ciliated neurons were activated under the stimulation of 10−5 mol/L amino acid mixture and 0.005× H. verticillata extract. The calcium signal imagery demonstrated that the nerve signals of the olfactory bulb were activated by the food odors. The quantitative real-time PCR (qPCR) analysis revealed significant changes in the expression of representative olfactory receptor genes in the olfactory bulb and brain of blunt snout bream upon stimulation with food odor. Collectively, these results provided evidence and preliminary explanations for the mediating role of olfaction in the feeding behavior of fish.

Transfer Learning in Multimodal Sunflower Drought Stress Detection

Efficient water supply and timely detection of drought stress in crops to increase yields is an important task considering that agriculture is the primary consumer of water globally. This is particularly significant for plants such as sunflowers, which are an important source of quality edible oils, essential for human nutrition. Traditional detection methods are labor-intensive, time-consuming, and rely on advanced sensor technologies. We introduce an innovative approach based on neural networks and transfer learning for drought stress detection using a novel dataset including 209 non-invasive rhizotron images and 385 images of manually cleaned sections of sunflowers, subjected to normal watering or water stress. We used five neural network models: VGG16, VGG19, InceptionV3, DenseNet, and MobileNet, pre-trained on the ImageNet dataset, whose performance was compared to select the most efficient architecture. Accordingly, the most efficient model, MobileNet, was further refined using different data augmentation mechanisms. The introduction of targeted data augmentation and the use of grayscale images proved to be effective, demonstrating improved results, with an F1 score and an accuracy of 0.95. This approach encourages advances in water stress detection, highlighting the value of artificial intelligence in improving crop health monitoring and management for more resilient agricultural practices.