Cariogenic Diet Research Articles

Prevalencia de hábitos bucodentales saludables en la población infanto-juvenil residente en España

IntroductionOral health is integral to children's health. To achieve this, it is essential establishing healthy habits early on them, such as regular check-ups, proper and frequent tooth brushing and a non-cariogenic diet. Prevalence of these habits are investigated in Spanish children by age group (pre-school: 2-5 years and school: 6-15 years) and by maternal educational level. Material and methodsA descriptive cross-sectional study was conducted, based on the last Spanish National Health Survey (2006). A representative sample of 8042 subjects (2-15 years) was obtained. ResultsA total of 47.35% reported having gone to dental services during the last year and 69.09% having gone sometime during their lives. Only 22.68% of the sample reported daily tooth brushing three times or more and 89.03% reported eating a cariogenic risk diet. In the school group (6-15 years) 29.87% of children whose mothers were university level brushed their teeth three times or more compared to 25.83% and 20.89% of those whose mothers had secondary and even primary studies respectively. For the intake of cariogenic risk diet, the percentages were 87.05%, 91.11% and 90.44%. In both age groups, and except for the consumption of sweets, there was a statistically significant association between maternal educational level and healthy habits. ConclusionsThe prevalence of these healthy habits is far lower than the recommendations: a lower use of services and a lower frequency of daily tooth brushing and higher intake of a cariogenic risk diet. The outcomes are more favourable in both age groups when the mother are university level, wich may serve as a guide to the promotion of oral health.

Dental Caries in Rats Associated with Candida albicans

In addition to occasional opportunistic colonization of the oral mucosa, Candida albicans is frequently found in carious dentin. The yeast’s potential to induce dental caries as a consequence of its pronounced ability to produce and tolerate acids was investigated. Eighty caries-active Osborne-Mendel rats were raised on an ampicillin-supplemented diet and exposed to C. albicans and/or Streptococcus mutans, except for controls. Throughout the 28-day test period, the animals were offered the modified cariogenic diet 2000a, containing 40% various sugars. Subsequently, maxillary molars were scored for plaque extent. After dissection, the mandibular molars were evaluated for smooth surface and fissure caries. Test animals exposed to C. albicans displayed considerably more advanced fissure lesions (p < 0.001) than non-exposed controls. While S. mutans yielded similar results, a combined association of C. albicans and S. mutans had no effect on occlusal caries incidence. Substituting dietary sucrose by glucose did not modify caries induction by C. albicans. However, animals fed a diet containing 20% of both sugars showed no differences to non-infected controls. Smooth surface caries was not generated by the yeast. This study provides experimental evidence that C. albicans is capable of causing occlusal caries in rats at a high rate.

A LATINO ORAL HEALTH PARADOX? USING ETHNOGRAPHY TO SPECIFY THE BIOCULTURAL FACTORS BEHIND EPIDEMIOLOGICAL MODELS

This article presents evidence of a "Latino oral health paradox," in which Mexican immigrant parents in California's Central Valley report having had better oral health status as children in Mexico than their U.S.-born children. Yet little research has explored the specific environmental, social, and cultural factors that mediate the much-discussed "Latino health paradox," in which foreign-born Latinos paradoxically enjoy better health status than their children, U.S.-born Latinos, and whites. Through ethnography, we explore the dietary and environmental factors that ameliorated immigrant parents' oral health status in rural Mexico, while ill-preparing them for the more cariogenic diets and environments their children face in the U.S. We argue that studies on the "Latino health paradox" neglect a binational analysis, ignoring the different health status of Latino populations in their sending countries. We use the issue of immigrant children's high incidence of oral disease to initiate a fuller dialogue between U.S.-based studies of the "health paradox" and non-U.S. based studies of the "epidemiological transition." We show that both models rely upon a static opposition between "traditional" and "modern" health practices, and argue that a binational analysis of the processes that affect immigrant children's health can help redress the shortcomings of epidemiological generalizations.

Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats

This study evaluated the influence of Psidium cattleianum Sabine (Myrtaceae) and Myracrodruon urundeuva Allemão (Anacardiaceae) aqueous extracts on S. mutans counts and dental enamel micro-hardness of rats submitted to a cariogenic challenge. Sixty Wistar rats were distributed in three groups and received water (control) or aqueous extracts of Psidium cattleianum or Myracrodruon urundeuva as hydration solution. Initially the animals had their sublingual and submandibular salivary glands surgically removed and the parotid ducts ligated. Then the rats were inoculated with 106 CFU of Streptococcus mutans ATCC 35668 and were fed with a cariogenic diet. To detect and quantify the presence of S. mutans, oral biofilms were sampled and microbial DNA was extracted and submitted to amplification by means of real-time PCR (Polymerase Chain Reaction). After seven weeks the animals were sacrificed and enamel demineralization was analyzed by cross-sectional micro-hardness. Both extracts produced a significant reduction on S. mutans counts and decreased the enamel demineralization. It can be concluded that the extracts tested had a significant effect on S. mutans in oral biofilm of the rats, decreasing S. mutans accumulation and enamel demineralization.

Heterogeneous Glycopeptides from Gastric Mucin Inhibit Dental Decay in Rats: A Pilot Study

ABSTRACT The aim of this pilot study was to investigate whether the application of sufficient amounts of soluble adhesin receptor analogues in the oral cavity could effectively inhibit the lectin mediated binding of Strep. mutans, and consequently the development of dental caries in vivo. Germ free rats were orally infected with Strep. mutans and were fed a high cariogenic diet. The control-group was given sterilized tap water. The test group received a sterilized solution of hydrolyzed gastric mucin. The test group developed significantly less carious lesions on approximal surfaces. Our data revealed that a continuous supplementation of glycopeptides from gastic mucins via drinking water is a potential inhibitor of dental decay in gnotobiotic rats.

Oral health and the postcontact adaptive transition: A contextual reconstruction of diet in Mórrope, Peru

This work explores the effects of European contact on Andean foodways in the Lambayeque Valley Complex, north coast Peru. We test the hypothesis that Spanish colonization negatively impacted indigenous diet. Diachronic relationships of oral health were examined from the dentitions of 203 late-pre-Hispanic and 175 colonial-period Mochica individuals from Mórrope, Lambayeque, to include observations of dental caries, antemortem tooth loss, alveolar inflammation, dental calculus, periodontitis, and dental wear. G-tests and odds ratio analyses across six age classes indicate a range of statistically significant postcontact increases in dental caries, antemortem tooth loss, and dental calculus prevalence. These findings are associated with ethnohistoric contexts that point to colonial-era economic reorganization which restricted access to multiple traditional food sources. We infer that oral health changes reflect creative Mochica cultural adjustments to dietary shortfalls through the consumption of a greater proportion of dietary carbohydrates. Simultaneously, independent skeletal indicators of biological stress suggest that these adjustments bore a cost in increased nutritional stress. Oral health appears to have been systematically worse among colonial women. We rule out an underlying biological cause (female fertility variation) and suggest that the establishment of European gender ideologies and divisions of labor possibly exposed colonial Mochica women to a more cariogenic diet. Overall, dietary change in Mórrope appears shaped by local responses to a convergence of colonial Spanish economic agendas, landscape transformation, and social changes during the postcontact transition in northern Peru. These findings also further the understandings of dietary and biocultural histories of the Western Hemisphere.

Oral Therapeutic Vaccination withStreptococcus sobrinusRecombinant Enolase Confers Protection against Dental Caries in Rats

Dental caries is among the more prevalent chronic human infections for which an effective human vaccine has not yet been achieved. Enolase from Streptococcus sobrinus has been identified as an immunomodulatory protein. In the present study, we used S. sobrinus recombinant enolase (rEnolase) as a target antigen and assessed its therapeutic effect in a rat model of dental caries. Wistar rats that were fed a cariogenic solid diet on day 18 after birth were orally infected with S. sobrinus on day 19 after birth and for 5 consecutive days thereafter. Five days after infection and, again, 3 weeks later, rEnolase plus alum adjuvant was delivered into the oral cavity of the rats. A sham-immunized group of rats was contemporarily treated with adjuvant alone. In the rEnolase-immunized rats, increased levels of salivary IgA and IgG antibodies specific for this recombinant protein were detected. A significant decrease in sulcal, proximal enamel, and dentin caries scores was observed in these animals, compared with sham-immunized control animals. No detectable histopathologic alterations were observed in all immunized animals. Furthermore, the antibodies produced against bacterial enolase did not react with human enolase. Overall, these results indicate that rEnolase could be a promising and safe candidate for testing in trials of vaccines against dental caries in humans.

Effects of Erosive, Cariogenic or Combined Erosive/Cariogenic Challenges on Human Enamel

Individuals with cariogenic diet can also consume erosive beverages. Thus, it seems necessary to investigate a possible caries/erosion interaction. To test in situ/ex vivo a combination of these challenges, 11 subjects wore intraoral appliances containing four enamel blocks randomly assigned. In the first 2-week phase, the appliances were immersed in a cola drink 3 times/day. Two blocks were free of plaque (erosion only: EO) and two blocks were covered with plaque (erosion + plaque: EP). In the second 2-week phase, four new blocks were all covered with plaque and subjected to a sucrose solution 8 times/day. Among the four new blocks, two were also subjected to the cola drink 3 times/day (erosion + caries: EC) while the other two were not (caries only: CO). Thus, in EO, the specimens were fixed at the intraoral appliance level. In EP, EC and CO they were fixed 1.0 mm under the appliance level and covered with plastic meshes for dental plaque accumulation. Changes in wear and hardness were measured. Data were tested using ANOVA and Tukey’s test (p < 0.05). Mean values of wear (µm) and change in hardness (kp/mm²) were: EO 4.82/310; EP 0.14/48; EC 0.34/245; CO 0.42/309. With respect to surface softening, EP and EC differed significantly from each other and from EO and CO, which did not differ significantly. EO presented significantly higher wear than the other groups. The data suggest that the presence of dental plaque can decrease the acid attack of an erosive drink and the association of erosive and cariogenic challenges showed less enamel alterations when compared to erosive or cariogenic challenges only.

Nutrition, diet and dental health — de- and remineralisation of teeth

There is little if any evidence that nutrition during the time of tooth formation has any clinically significant effect on caries susceptibility of erupted teeth in man. Caries, erosion and demastication are not nutritional diseases. They are, however, diet-related diseases because erupted teeth are subject to local chemical and mechanical effects of the diet contacting the teeth. Caries is the result of a chronic undermining demineralisation of the teeth by organic acids that are produced by the bacteria of the dental biofilm while fermenting carbohydrates from the human diet. Dental erosion is the result of a chronic localised loss of dental hard tissue etched away from the surface by acids derived from the diet or from gastric reflux. The physicochemical grounds of de- and remineralisation of teeth are explained using detailed diagrams. Initial caries lesions without cavitation of the surface can remineralise (heal) under conditions of low cariogenic diet and good oral hygiene. However, once the surface has broken and cavitation occurred, there is no alternative to restorative dental therapy because remineralisation is no more possible.

Ernährung, Nahrungsmittel und Zahngesundheit – De- und Remineralisation der Zähne

There is little if any evidence that nutrition during the time of tooth formation has any clinically significant effect on caries susceptibility of erupted teeth in man. Caries, erosion and demastication are not nutritional diseases. They are, however, diet-related diseases because erupted teeth are subject to local chemical and mechanical effects of the diet contacting the teeth. Caries is the result of a chronic undermining demineralisation of the teeth by organic acids that are produced by the bacteria of the dental biofilm while fermenting carbohydrates from the human diet. Dental erosion is the result of a chronic localised loss of dental hard tissue etched away from the surface by acids derived from the diet or from gastric reflux. The physicochemical grounds of de- and remineralisation of teeth are explained using detailed diagrams. Initial caries lesions without cavitation of the surface can remineralise (heal) under conditions of low cariogenic diet and good oral hygiene. However, once the surface has broken and cavitation occurred, there is no alternative to restorative dental therapy because remineralisation is no more possible.

Designing school programmes to be effective vehicles for changing oral hygiene behaviour

Schools are a commonly used setting for dental health education, health promotion and interventions with preventive agents. However, traditional dental health education programmes are rarely designed to be effective change agents. Twice daily tooth brushing with fluoride toothpaste has consistently been shown to be an important behaviour for controlling dental caries in children. Ideally, parents incorporate twice daily brushing into their child’s hygiene routine. Families where brushing is irregular are over-represented in areas of socio-economic deprivation and the effects of irregular oral hygiene are often compounded by cariogenic diets. Therefore, in communities with high caries levels, a targeted community approach to prevention can provide additional benefit, through fluoride programmes delivered in school. It is essential that dental health education programmes are designed to recognise the social, cultural and environmental context in which the behaviours occur. This paper provides a new framework to classify school programmes. In addition, evidence-based guidance is developed of the necessary components for programmes to enhance effectiveness in establishing the key behaviour of twice-daily tooth brushing with fluoridated toothpaste.

The influence of a novel propolis on mutans streptococci biofilms and caries development in rats

A flavonoids-free Brazilian propolis (type 6) showed biological effects against mutans streptococci and inhibited the activity of glucosyltransferases. This study evaluated the influence of the ethanolic extract of a novel type of propolis (EEP) and its purified hexane fraction (EEH) on mutans streptococci biofilms and the development of dental caries in rats. The chemical composition of the propolis extracts were examined by gas chromatography/mass spectrometry. The effects of EEP and EEH on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 biofilms were analysed by time-kill and glycolytic pH drop assays. Their influence on proton-translocating F-ATPase activity was also tested. In the animal study, the rats were infected with S. sobrinus 6715 and fed with cariogenic diet 2000. The rats were treated topically twice a day with each of the extracts (or control) for 5 weeks. After the experimental period, the microbial composition of their dental plaque and their caries scores were determined. The results showed that fatty acids (oleic, palmitic, linoleic and stearic) were the main compounds identified in EEP and EEH. These extracts did not show major effects on the viability of mutans streptococci biofilms. However, EEP and EEH significantly reduced acid production by the biofilms and also inhibited the activity of F-ATPase (60-65%). Furthermore, both extracts significantly reduced the incidence of smooth surface caries in vivo without displaying a reduction of the percentage of S. sobriuns in the animals' plaque (P < 0.05). However, only EEH was able to reduce the incidence and severity of sulcal surface caries (P < 0.05). The data suggest that the cariostatic properties of propolis type 6 are related to its effect on acid production and acid tolerance of cariogenic streptococci; the biological activities may be attributed to its high content of fatty acids.

Cariogram – a multifactorial risk assessment model for a multifactorial disease

This paper reviews some common methods for the assessment of caries risk. It also describes a new way of illustrating the caries risk profile of an individual, the Cariogram. Past caries experience and socioeconomic factors are often used for prediction of caries. As prediction models, the methods are simple, inexpensive and fast. However, they are not risk models, as they do not specify which particular risk factors are operating. Various biological factors can be used for risk assessment. Common ones are bacteria, diet and host factors. Taken separately, these biological factors often have limited predictive values. Socioeconomic factors often have a heavy impact on the biological factors as they can explain why an individual, for example, has a cariogenic diet or neglects oral hygiene. The biological factors are the immediate cause of the cavities. Caries experience is an illustration of how the host copes up with the biological activity. To facilitate the interpretation of biological data, the Cariogram was developed. It is a computer program showing a graphical picture that illustrates a possible overall caries risk scenario. The program contains an algorithm that presents a 'weighted' analysis of the input data, mainly biological factors. It expresses as to what extent different etiological factors of caries affect caries risk. The Cariogram identifies the caries risk factors for the individual and provides examples of preventive and treatment strategies to the clinician.

Análise dos livros didáticos do ensino fundamental e médio quanto aos conteúdos de saúde bucal

The school should offer the information about the health care. Children and teenagers have the opportunity to know, to explore, and to fix concepts with regard to the body care, including in this context the oral health. The purpose of this text is to analyze the information in the elementary and high school didactics books used by the public and private institutions in Santa Maria. A total of 48 books were analyzed, through content analysis, and the collected information was registered in a previous developed card. The subject referred to dental caries, periodontal diseases, fluoride use, cariogenic diet, and the dentist were qualified in terms of satisfaction and dissatisfaction according to scientific criteria. The results indicated that there is the predominance of the dissatisfaction criteria, mainly in what refers to the dental caries and the periodontal diseases information, whose contents can induce the wrong and incomplete knowledge. According to the results obtained by this work, it is possible to conclude that there is necessity of adequacy of the oral health didactics contents that are presented in the books adopted by public or private schools. In this sense, the dentistry should subsidize book authors and schools with information that are adequate to the scientific point of view, and it also should work beside teachers to be more useful with their students.

Breast‐feeding and early childhood caries: an assessment among Brazilian infants

The purpose of the present study was to evaluate early childhood caries among 12-36-month-old children from families living in poor socio-economic conditions in the city of Recife, Brazil, its association with the type and duration of feeding (e.g. natural, sugared, bottle and glass), as well as the relationship between a supplementary diet and the occurrence of this type of caries. The present study consisted of a visual clinical examination of teeth that had been previously cleaned with gauze. This was carried out under natural light in a waiting room. Four calibrated examiners performed the examination and the kappa test value was 8.0. The parents or guardians were interviewed for the following information: name, address, age, type of feeding, number of sugary meals, sugar intake and habitual diet. Some 468 children were included in this study. Their ages ranged from 12 to 36 months. The sample was comprised of 222 (47.4%) males and 246 females (52.6%). Of the 468 children included in this study, 133 (28.4%) had caries. Only 59 (12.6%) of the children examined had been breast-fed, 20 (33.9%) of whom presented with caries. Three hundred and twenty-seven (69.9%) subjects had been bottle-fed with sugared milk, 86 (26%) of whom had caries. Two hundred and eight children had five or more sugary meals per day, 70 (33.6%) of whom had caries. No statistically significant relationship was seen between breast-feeding and the prevalence of tooth decay. The results of the present study show that the prevalence of early childhood caries in 12-36-month-old children from poor backgrounds in Recife is in accordance with the rate found in other Brazilian cities and is extremely high compared with that of the world population as a whole. Early childhood caries was not clearly related to the type of feeding in this sample. The prevalence of early childhood caries increased with age, and the number of sugary snacks between meals and a cariogenic diet were strongly related to early childhood caries. The lack of fluoridated water and high rates of early childhood caries in lead the authors to suggest that fluoride dentifrice should be introduced at 12 months of age as a fluoride supplement and an important aid in the prevention of childhood caries. Additional studies in different cultures and societies need to be undertaken before a definitive conclusion can be drawn.

The effect of fluorine and homeopathic medicines in rats fed cariogenic diet

Although some sectors of dentistry have benefited from technological advances, dental caries is still a major problem. Prevention and treatment of dental caries by fluorine is considered a major advance in public health. Nevertheless fluorosis, caused by ingestion of excessive amounts of fluorine during the period of teeth formation, is of great concern. In accordance with the homeopathic doctrine, minimum doses of fluorine and other substances could prevent and/or treat caries. In this experiment, we compared the preventive action of fluorine and evaluated the effect of homeopathic medicines on the teeth of rats fed a cariogenic diet. None of the groups included in this study developed caries. However, microscopy revealed the presence of precipitate and/or deposit in the groups treated with homeopathic medicines. This phenomenon might be due to deposit in the dental surface or precipitation of bacterial plaque or calcium salts. It was not possible to identify the composition of the deposit/precipitate due for technical reasons. In one of the groups treated with homeopathic medicines fur loss was observed in 40% of animals. These reactions might be caused due to the action of the homeopathic medicines.

A pilot study of cariogenic bacteria and diet associated with root surface caries in Iceland

A pilot study of cariogenic bacteria and diet associated with root surface caries in Iceland

Dental health and fluoride supplements on Tristan da Cunha

Background The island of Tristan Da Cunha is one of the few examples in the world of a remote enclosed community. The inhabitants of the island were transported to England in 1961 as a result of a volcanic eruption and during their time in England they received a detailed dental health examination. They were later examined back on the island in 1966 by Dr John Fisher and in 1982 a school fluoride supplementation programme was introduced. Aim The present paper reports the results of the first dental health survey to be carried out on the island since the introduction of a regular school fluoride supplementation programme in 1982. Subjects and methods A cohort of 6–19-year-old subjects on the island were examined in 1996 using a similar protocol to that which was used by Fisher in the 1966 examination. Results Comparing the 6–12-year-olds who were caries free in 1966 with those caries free in 1996 using the Chi-squared statistic reveals a statistically significant greater number caries free in the more recent cohort (χ2 = 6.0, p = 0.014). For the older age group (13–19 years), a similar comparison reveals a highly statistically significant difference (χ2 = 12.26, p = 0.005). Conclusions A significant increase in the number of caries free 6–19 year old children in Tristan Da Cunha between 1966 and 1996 was noted: 1) This is a significant finding in the light of the school fluoride supplementation programme that was introduced on the island in 1982; and 2) Appropriate fluoride supplementation regimes may have conferred a protective effect in a group of children with a cariogenic diet. The paper discusses the significance of this study in the support of fluoridation as a method of reducing the prevalence of dental caries and also discusses possibilities for future research on the island of Tristan Da Cunha.

Black tea extract and dental caries formation in hamsters

Several studies have suggested that green tea and Oolong tea extracts have antibacterial and anticariogenic properties in vitro and in vivo. The aim of the present study was to determine the effect of a standardized black tea extract (BTE) on caries formation in inbred hamsters on a regular and a cariogenic diet. Eighty hamsters were divided into four groups of 20 animals each. Two groups received a pelleted regular diet (LabChow) with water or BTE ad libitum. The other two groups received a powdered cariogenic diet (Diet 2000, containing 56% sucrose) with water or BTE ad libitum. The animals were kept for 3 months on their respective diets and then were sacrificed. The heads were retained, the jaws were prepared and stained using alizarin mordant red II, and were then scored for dental caries according to the Keyes method. This is the first study indicating that BTE, as compared with water, significantly decreased caries formation by 56.6% in hamsters on a regular diet and by 63.7% in hamsters on a cariogenic diet (P<0.05). In the cariogenic diet group BTE, reduced the mandibular caries score of the hamsters slightly more than the maxillary caries score. The fluoride content of the standardized BTE solution was frequently monitored during the experiment; the mean fluoride concentration was found to be 4.22 ppm. A frequent intake of black tea can significantly decrease caries formation, even in the presence of sugars in the diet.

Black tea extract and dental caries formation in hamsters.

Several studies have suggested that green tea and Oolong tea extracts have antibacterial and anticariogenic properties in vitro and in vivo. The aim of the present study was to determine the effect of a standardized black tea extract (BTE) on caries formation in inbred hamsters on a regular and a cariogenic diet. Eighty hamsters were divided into four groups of 20 animals each. Two groups received a pelleted regular diet (LabChow) with water or BTE ad libitum. The other two groups received a powdered cariogenic diet (Diet 2000, containing 56% sucrose) with water or BTE ad libitum. The animals were kept for 3 months on their respective diets and then were sacrificed. The heads were retained, the jaws were prepared and stained using alizarin mordant red II, and were then scored for dental caries according to the Keyes method. This is the first study indicating that BTE, as compared with water, significantly decreased caries formation by 56.6% in hamsters on a regular diet and by 63.7% in hamsters on a cariogenic diet (P < 0.05). In the cariogenic diet group BTE, reduced the mandibular caries score of the hamsters slightly more than the maxillary caries score. The fluoride content of the standardized BTE solution was frequently monitored during the experiment; the mean fluoride concentration was found to be 4.22 ppm. A frequent intake of black tea can significantly decrease caries formation, even in the presence of sugars in the diet.