Carioca Bean Research Articles

Aging indicators for stored carioca beans

High temperature, moisture content and radiation conditions, common in the tropics, accelerate the physiological post-harvest disorders in beans, affect integument color and bean hardness. This study explored the darkening and hardening mechanisms in carioca type beans during storage. The contrasting genotypes for bean darkening and hardening (BRS Estilo and BRS Pontal: rapid darkening and hardening; BRSMG Madrepérola and CNFC 10467: slow darkening and partially resistant to hardening; and a Canadian genotype of the Pinto Bean type resistant to darkening (negative control)) were evaluated right after harvest and after six months storage at 20.3 ± 0.2 °C and 78.9 ± 6.0% RH, with respect to their physicochemical, biochemical and morphological attributes. All the samples hardened with time, but the velocity of darkening did not always parallel the degree of hardening of the beans (that increase linearly with time), which indicates that the color of the bean integument was not a safe indicator to predict the culinary quality of carioca beans. During storage, the SOD (superoxide dismutase) activity and LP (lipid peroxidation) in the cotyledon increased, and the increase was more significant in the integument of the rapid-darkening genotypes. The oxidative stress detected in the beans apparently started in the green bean formation phase, continuing during post-harvest, especially for the rapid-darkening beans. Quantification of the bivalent ions in the bean fractions, together with the degree of membrane damage was positively associated with bean darkening, independent of the group. Thus, aging of the carioca beans could be triggered by a complex mechanism involving diverse intrinsic factors in different degrees according to the genotype and the post-harvest period, but some parameters could serve as indicators, as cooking time, hardness and color, to differentiate between rapid and slow darkening beans.

Physical quality and mineral composition of new Mesoamerican bean lines developed for cultivation in Brazil

The present study was undertaken to evaluate whether new common bean lines present differences in their physical quality and mineral composition traits; investigate correlations between those traits; and to select common bean lines for use in human nutrition and in breeding programs. Four experiments were carried out to examine 17 genotypes (cultivars and lines) of carioca beans (beige seed coat with brown streaks) and black beans, which represent the technological advances of six breeding programs in Brazil. A significant genotype × environment interaction was observed for all evaluated traits, except for potassium concentration. Most physical quality traits were correlated with each other (r ≥ 0.848), and almost all evaluated pairs of minerals exhibited a positive correlation with each other (r ≥ 0.609). Indirect selection based on the highest values for normal grains and uptake is efficient for identifying fast-cooking common bean lines, and it is possible to select mineral-biofortified common bean lines. The LP 09-33 and LEC 01-16 carioca bean lines and the TB 03-11 and CHP 01-182-48 black bean lines present high physical quality and better mineral composition, and their use in human nutrition and in breeding programs is therefore recommended.

Enzymatic hydrolysis of Carioca bean (Phaseolus vulgaris L.) protein as an alternative to commercially rejected grains

This study on Carioca bean protein aims to improve its functional properties by enzymatic hydrolysis at 6 and 9% degree of hydrolysis using the pH-stat method. The production of protein fractions of lower molar masses, evidenced by gel electrophoresis (SDS-PAGE), allowed an increased solubility at basic pH values, reduction in hydrophobicity, improvement in emulsion properties (emulsification capacity, emulsification activity, and emulsion stability), and foaming properties (foaming ability, rates of liquid incorporated into the foam and drainage). These parameters were studied for hydrolyzed and control samples at pH 3.0, 5.0, 7.0, and 9.0. Carioca bean protein hydrolysates presented higher values of solubility, hydrophobicity, emulsification capacity, and emulsifying activity compared to soy hydrolysates. It was also observed that foam formation and collapse behaviors were similar between hydrolyzed Carioca bean protein and soybean protein. It was concluded that Carioca bean protein has a potential to be used in food formulations when emulsion and foaming characteristics are desirable.

English

Pesticides are identified as hazardous contaminants due to high toxicity and persistence in the environment. In this context, it is necessary to monitor these pollutants in the environment in situ, with the use of low cost analytical techniques and of quick response. Biosensors are presented as a complementary analytical tool to more complex techniques, such as chromatography. The present work aims to develop, in a simple and low cost way, an amperometric biosensor for the detection of simazine in aqueous samples from the inhibition of the enzyme peroxidase extracted from the pinto bean (Phaseolus vulgaris L.). For the development of the biosensor, the enzyme peroxidase was extracted in sodium phosphate buffer solution, of pH 6.0, and pre-purified by ammonium sulfate precipitation 40% (w / v) salt saturation to 50.0 ml of the protein extract. Peroxidase was characterized by electrophoresis and immobilized on silica-titanium oxide, synthesized by the sol-gel method in the proportions of 70% / 30% respectively. The biosensor showed linearity in the concentration range between 1.0 and 6.0 μgL-1 of simazine (R2 = 0.989) in the potential range 0.20 V to 0.30 V vs Ag / AgCl by the square wave voltammetry technique. The curve was obtained in the presence of 2.0 mgL-1 phenol and 3% H2O2 (v / v)), where the current density signal decreased in the presence of successive additions of the simazine inhibitor. The biosensor was applied to a standard simazine-fortified sample and detected the presence of simazine at the concentration of 35.05 μgL-1. Even showing a relative percentage error of 12.63%, when compared with the result of the analysis by high performance liquid chromatography, the biosensor is useful to monitor the presence or absence of the simazine pesticide in the solution. Key words: Simazine, Biosensor, Carioca beans (Phaseolus vulgaris L.).

Induction of seed coat darkening in common beans (Phaseolus vulgaris L.) and the association with cooking time after storage

Carioca is the most important edible dry bean (Phaseolus vulgaris L.) grown in Brazil. It represents the largest dry bean market class in the world. The seed coat of carioca beans will darken under adverse harvest conditions and with the increasing of storage time. In general, darkened seeds are associated with older seeds that suffer from prolonged cooking times. A relatively new ‘slow darkening’ trait is available in carioca that delays seed coat darkening under storage. However, its effect on cooking time is unknown. The objective of this work was to evaluate two induction methods of seed coat darkening and to examine the effect of slow darkening trait on cooking time after storage. Lines derived from four segregating populations resulting from crossings between cultivar BRSMG Madrepérola with slow seed coat darkening, and the parents BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime with normal darkening were evaluated. An experiment inlcuding 220 lines, 55 per population and the five parents, in a 15x15 triple lattice was conducted in the winter growing season in Brasilia. Seed coat darkening and cooking time traits were evaluated. Two methods for inducing seed coat darkening were compared: an accelerated aging test using UV light for 72 hours, and an extended storage for 90 days under ambient conditions. The correlation between the induction methods ranged from 0.77 to 0.85 for the different populations indicating either method could be used to discriminate lines with normal versus slow darkening trait. The percentage of light-colored grain lines was identified by both induction methods of seed coat darkening ranged from 75 to 85.7% in the populations. The genetic correlation between seed coat darkening and cooking time varied from -0.06 to -0.48, indicating that in some populations there is no significant genetic correlation between seed coat darkening and cooking time. Thus, light colored grains are not indicative of low cooking time, when they are considered genotypes with genetic variation for the seed coat darkening.

A half century of a bean breeding program in the South and Alto Paranaíba regions of Minas Gerais

Abstract The success of bean (Phaseolus vulgaris L.) crops in Brazil is due to the development of new cultivars. The aim of the present study was to critically analyze the fifty years of a bean breeding program at the Universidade Federal de Lavras (UFLA) and estimate the genetic progress (GP) achieved for grain yield. The estimated genetic progress for grain yield was 0.35% per year, which is associated with visible improvement in plant architecture, resistance to some pathogens, and better carioca bean grain quality. The program was found to be efficient, generating 119 theses and 43 dissertations during the period under study. In addition, hundreds of articles have been published, especially studies aimed at improving the efficiency of genetic breeding programs. Over the past few years, partnerships with other public institutions in performing Value for Cultivation and Use tests have resulted in 12 recommended cultivars.

Nutrition, yield and nutrient export in common bean under zinc fertilization in no-till system

ABSTRACT Zinc (Zn) is one of the most deficient plant micronutrients in agricultural crops. The objective of this study was to evaluate plant nutrition, grain yield and nutrient export rate in response to soil and foliar Zn fertilization in common bean (Phaseolus vulgaris L.). Two field experiments in no-till system were carried out using two common bean cultivars, BRS Esteio (black bean) and IPR Campos Gerais (Carioca bean). Treatments were composed of soil Zn application during sowing and foliar Zn spray at flowering stage. Soil Zn application had effect on leaf Zn concentration in IPR Campos Gerais and did not affect grain yield of both cultivars. Foliar Zn spray increased leaf Zn concentration by approximately two times in both cultivars, but negatively affected the grain yield in BRS Esteio. Leaf concentration of N, Ca and S were affected by soil Zn application and leaf concentration of Mn was affected by foliar Zn spray, while leaf concentration of P, K, Mg, Cu and Fe were not influenced by the soil and foliar Zn treatments. In treatments without Zn, the descending order of nutrient export rate from the experimental site was as follows: N > K > P > Ca ≈ S > Mg for macronutrients and Fe > Mn > Cu > Zn for micronutrients. Foliar Zn spray increased the export rate of Zn, P, Ca, Mg, S, Mn, Cu and Fe in IPR Campos Gerais, while soil Zn application resulted in higher export rate of P, K and Mn in BRS Esteio.

Scanning Electron Microscopy and Crystallinity of starches granules from cowpea, black and carioca beans in raw and cooked forms

Abstract Beans are a vital food for the population around the world because it contains a relatively high amount of protein, vitamins, fiber, and minerals, being an important vegetable source of iron, particularly when combined with ascorbic acid and cysteine. The starch content in the seed is between 45 and 60%. This paper evaluated the granular structure of starches from common beans (Phaseolus vulgaris) and cowpea (Vigna unguiculata) in raw and cooked forms by Scanning Electron Microscopy (SEM), and their crystallinity by X-ray diffraction (DR-X). By SEM, was observed the occurrence of gelatinization phenomenon in cowpea and carioca beans, and the resistance to this event in black beans. By DR-X, the diffraction angles found in this work are more consistent with the classification of the standard polyphorm A. The Relative Crystallinity (RC) of black bean cowpea and carioca beans, observed by X-ray diffraction, varied significantly.

Combining Ionizing Irradiation and Ultrasound Technologies: Effect on Beans Hydration and Germination.

The present work studied, for the first time, the effect of two technologies on the hydration and germination process of beans: ionizing irradiation and ultrasound. In addition, this work proposed a possible biological effect of ultrasound to describe its effect on grain hydration. Carioca beans were irradiated at doses of 2.3 and 3.8 kGy using γ-rays, establishing different metabolic activities for the beans. Then, they were hydrated with and without the ultrasound technology (91 W/L and 25 kHz) at 25°C. Both the hydration and germination kinetics were evaluated considering the application of both technologies alone and in combination. As results, irradiation did not significantly affect (P>0.05) the hydration rate, the equilibrium moisture, or the lag phase time, but ultrasound affected them, reducing 50% of the processing time. On the other hand, only the equilibrium moisture was slightly reduced by the interactive effect of irradiation and ultrasound technology, which was discussed based on different metabolism levels. Furthermore, the germination process of carioca bean was hindered by both the studied variables, reducing its germination capacity to 0% in some cases. Therefore, by studying the effect of ultrasound on beans with different active metabolism (due to different irradiation doses), it was suggested that both physical and biological mechanisms are involved during hydration process and that both can be affected by ultrasound. Further, irradiation could be used on carioca beans with objectives such as avoid germination, insects, and microorganism's growth, without an important effect on the hydration kinetics. PRACTICAL APPLICATION: Ultrasound technology has demonstrated to accelerate the soaking process of several legume grains. However, sometimes, grains are irradiated for disinfestation purpose to improve their shelf life. Indeed, irradiation can change the grain properties as the cooking time, hydration time, and germination capacity. Therefore, this work verified if irradiation changes the desirable effect of ultrasound on grain processing: soaking and germination. Moreover, this work novelty is also based on describing the phenomena: by proposing a possible biological effect, further approaches to improve the grain processing can be achieved.

Physicochemical and technological properties of common bean cultivars (Phaseolus vulgaris L.) grown in Brazil and their starch characteristics

A great diversity of bean cultivars is developed each year, but, to our knowledge, there is a dearth of information regarding the physicochemical and technological properties of the grains, as well as its starch characteristics. Therefore, this study aims to evaluate the physicochemical and technological properties of five cultivars from the carioca bean group and five cultivars from the black bean group as freshly harvested grains. Moreover, the physicochemical, crystallinity and pasting properties of isolated starch were also determined. The results showed differences in all of the studied properties as a function of genotype. High grain thickness and low seed coat percentage were associated with the low cooking time. The highest protein and starch content were exhibited by grains from the “Perola” and “Estilo” cultivars, respectively. Similar crystalline structure was observed in all starches, but the relative crystallinity and the physicochemical and pasting properties were dependent on the cultivar.

Gamma irradiation of common beans: Effect on nutritional and technological properties

This work aimed studied the effect of ionizing irradiation on the nutritional and technological properties of cooked Carioca beans (Phaseolus vulgaris). The dry beans were irradiated with different doses (1, 5 and 10 kGy) of γ-radiation using 60Co as source. The composition and nutritional properties (protein, fat, ash, tannin, phytic acid content and protein in vitro digestibility) of the cooked beans were analyzed, with and without previously soaking process. In addition, the technological properties (hydration index, cooking time and color) of the beans were also evaluated. The results showed that protein, fat, ash and tannin content, as well as color, were not affected by irradiation, while the phytic acid was significantly reduced by the irradiation. Further, hydration index was slightly reduced only by using 10 kGy suggesting probable structure changes. Irradiation reduced the cooking time of the beans, as higher irradiation doses were used, which was related with the reduction on the phytic acid content. Moreover, protein in vitro digestibility was enhanced by irradiation. In conclusion, irradiation up to 10 kGy can be safely used to improve the shelf life of common beans without degradation of nutritional components, reducing some antinutrients content, improving protein digestibility and reducing the cooking time.

Genetic Control of Seed Coat Darkening in Common Bean Cultivars from Three Market Classes

Common bean (Phaseolus vulgaris L.) cultivars with slow seed coat darkening benefit the growers by extending the seed storage period without losses in commercial quality. Seed coat darkening in pinto and carioca seeded common bean is controlled by recessive genes, but it is not known whether these genes are different or not. The objectives of this study were (i) to verify if the gene that controls seed coat darkening in common bean cultivars from different market classes is the same, and (ii) to evaluate the efficiency of the simple sequence repeat (SSR) marker Pvsd‐1158 and the single nucleotide polymorphism (SNP) marker PvbHLHp12804 linked to the Sd gene in identifying carioca and mulatinho seeded lines contrasting for seed coat darkening. Seventeen lines were used for genotypic and phenotypic evaluations in two trials, and F2:3 progenies obtained from crosses between one pinto bean line (Sd gene) and two carioca lines that have slow seed coat darkening were used in segregation tests. The result of the two markers was identical and shown 87.5% coincidence with phenotypic data. Only two lines, CNFM 11940 (mulatinho) and TAA Dama (carioca), exhibited slow seed coat darkening and marker alleles related to regular darkening. Therefore, there was recombination between the markers and the Sd gene, or there is another gene causing slow seed coat darkening in these lines. All F2:3 progenies exhibited slow seed coat darkening, indicating that there was no segregation. The two markers are efficient for assisted selection. Results of the genotypic evaluations and the segregation test indicating that the same gene (Sd) is responsible for seed coat darkening in pinto and carioca beans.

Generation and validation of genetic markers for the selection of carioca dry bean genotypes with the slow-darkening seed coat trait

Slow darkening (SD) is a trait that helps to maintain a brighter seed coat appearance in certain market classes of dry beans. The aim of this study was to generate new fluorescence-based markers and validate previously identified microsatellite markers for linkage to the SD trait in lines of the carioca market class. Four segregating populations were generated by Embrapa, the Brazilian Agricultural Research Corporation, from crosses between the SD cultivar BRSMG Madrepérola and the regular-darkening cultivars BRS Estilo, BRS Cometa, BRS Notável and BRS Sublime. These populations were screened with the simple-sequence markers Pvsd-1158 and PVM02TC116 and with a TaqMan™ marker designed for the single-nucleotide polymorphism (SNP) PvbHLHp12804. A KASP marker was also designed for the PvbHLHp12804 marker for testing on advanced carioca lines developed by the University of Saskatchewan. In the carioca lines developed by Embrapa, PVM02TC116 proved unsuitable for marker-assisted selection (MAS). Both the Pvsd-1158 and PvbHLHp12804 markers were found to be tightly linked to the gene responsible for the SD trait, with genetic distances calculated at 2.8 cM for Pvsd-1158 and 2.0 and 3.1 cM for PvbbHLHp12804, respectively. These markers presented more than 97% of selection efficiency. The genotypic scoring using the PvbHLHp12804 KASP marker was perfectly correlated with the phenotype in all lines of the University of Saskatchewan. The results of this study validates the use of Pvsd-1158 as a gel-based marker for SD in carioca beans. The new fluorescence-based SNP PvbHLHp12804 markers exhibited very tight linkage to SD in carioca and pinto bean lines. These markers will be ideal for MAS for the SD trait in these market classes.

GGE Biplot and REML/BLUP based-analysis of yield stability and adaptability for common beans in multi-environment trials

Prior to releasing new varieties, one of the most common aims in breeding is to study genotype by environmental interactions. Using the analysis obtained from these initial processes, a multitude of varieties based on their relative performance through different environments is produced. Herein we assessed the link-up between GGE Biplot and REML/BLUP based methods in a commercial panel of 32 bean genotypes, which included 17 carioca bean genotypes and 15 black bean genotypes. The assessment was conducted during 2009 in four different locations of Pernambuco State, Brazil. Regarding the locations, Sao Joao and Araripina showed to be highly representative and non-discriminatory, while Arcoverde was the most discriminatory and well-represented favoring the selection of widely adapted genotypes. The selection of genotypes was consistent by using both methods and the final conclusion shows outstanding performance. Considering a high yield stability and adaptability, the following genotypes showed outstanding performance: the black types included CNFP (11995, 11983, 11985 and 11991), and the carioca types are CNFC (11951 and 11954). Understanding genotype by environmental interactions enabled us to identify potential genotypes for releasing.

Effects of refrigeration on biochemical, digestibility, and technological parameters of carioca beans during storage.

The objective of this study was to evaluate the effects of grain moisture, temperature, and storage time on biochemical, digestibility, and technological parameters of carioca beans. The grains were stored at 16.7% and 13.8% of moisture at 12, 20, 28, and 36°C for 240days. It was observed that a reduction in the storage temperature maintained the germination and vigor of the grains. The temperature of 36°C causes between 3.81% and 4.52% reduction in weight of a thousand grains and significant darkening. Carioca beans stored at 36°C exhibited hard-to-cook (HTC) defect at 80days and increases in hardness after 240days. The best digestibility indexes of carioca beans were obtained with refrigerated storage. The refrigerated storage (12°C) provides the best preservability of the grains throughout the storage, verified by the parameters biochemical, digestibility, and technological parameters. PRACTICAL APPLICATIONS: Carioca beans are among the most consumed beans in Brazil and their quality for consumption is directly influenced by storage conditions that alter color, nutritional value, bioactive, and cooking properties. Thus, this study seeks to present a practical and viable solution for stockist of bean grains, so that they can store these grains for long periods, maintaining the quality standards for commercialization. In addition, this technology allows the stockist to identify the best time for commercialization of the carioca beans, which has high added value.

Soluble extracts from carioca beans (Phaseolus vulgaris L.) affect the gut microbiota and iron related brush border membrane protein expression in vivo (Gallus gallus).

The effect of soluble extracts with putative prebiotic ability extracted from various bean varieties on the intestinal brush border membrane (BBM) iron related proteins, and intestinal bacterial populations were evaluated using the Gallus gallus model and by the intra-amniotic administration procedure. Eight treatment groups [(non-injected; 18 MΩ H2O; 40 mg/mL Inulin; 50 mg/mL BRS Perola (carioca standard); 50 mg/mL BRS Cometa (carioca, Fe biofortified); 50 mg/mL BRS Esteio (black, standard); 50 mg/mL SMN 39 (black, Fe biofortified); 50 mg/mL BRS Artico (white, standard)] were utilized. Tested groups reduced the relative abundance of Clostridium and E. coli compared to the Inulin group (positive control) and they did not affect the relative abundance of Bifidobacterium and Lactobacillus compared to the negative control (18MΩ H2O). The relative expression of zinc transporter 1, ferroportin and amino peptidase were up-regulated in the BRS Cometa group (Fe-biofortified carioca beans). Results suggest that soluble extracts from carioca beans may improve the iron bioavailability by affecting intestinal bacterial populations, and BBM functionality.

Evaluation of Lipoxygenase Activity in Common Beans by UV and NMR Spectroscopy: Proposal for a Complementary Technique for Enzymatic Studies

Factors that reduce the quality of common bean during storage, including oxidative rancidity caused by lipoxygenase, have been extensively studied. In this context, this work evaluates lipoxygenase activity in five Carioca bean cultivars at different temperatures and storage times using ultraviolet (UV) and nuclear magnetic resonance (NMR) spectroscopies. UV absorption analyses demonstrated that the storage temperature of the grains influences the kinetic reaction rates during the enzymatic activity of lipoxygenase. Pinto beans and CNFC 10467 presented lower initial activity than the three BRS varieties but were more sensitive to high temperatures. BRS Estilo presented an anomalous behavior, with a strong reduction in activity at 15 and 21 °C until 72 days of storage. BRSMG Madreperola and BRS Pontal presented stable enzymatic rates until 108 days of storage. A 26% reduction in fatty acid content, as a result of lipoxygenase action during storage, was observed both in solution-state and high resolution magic angle spinning (HR-MAS) NMR studies. These results highlight the importance of NMR in enzymatic kinetic reactions studies, complementing the results obtained by UV.

Consumer preference and the technological, cooking and nutritional quality of carioca beans

Carioca beans will achieve better consumer acceptability if they exhibit high grain quality. The objectives of this work were to identify the visual quality traits that determine consumer choice of carioca beans; to evaluate whether the commercial brands of carioca beans present differences in their technological, cooking and nutritional quality traits; and to select the best commercial brand of carioca beans according to these traits using a selection index. For this purpose, six different commercial brands of carioca beans were evaluated with respect to consumer preference and their grain physicochemical traits. The visual quality traits, colour and grain size, defined the consumer choice of carioca beans. The commercial brands of carioca beans presented differences regarding their technological, cooking and nutritional quality traits. Brand 4 had lighter colour grains (L* = 53.33), a slightly red shade (a* = 6.20), medium-sized beans (27.52 g), an elliptical shape, a semi-full flatness, and a fast cooking time (15 min and 25 s). The brand 4 showed high concentrations of protein, potassium, phosphorus, calcium, zinc and copper, and this sample was the preference of 51.92% of the survey participants. The best commercial brand of carioca beans selected by the selection index was brand 4 for its technological, cooking and nutritional quality traits.

Carioca bean genotypes for tolerance to grain darkening by natural and accelerated methods

ABSTRACT The slow darkening of grains is sought by bean breeders because the consumers consider that darker grains demand more time for cooking. The analysis currently used takes around 90 days to differentiate grain color among genotypes. The objective was to evaluate the color as a function of the value of L* (lightness) of carioca beans, by natural and accelerated methods to verify equivalence between methods, validation of the methodology and identification of genotypes tolerant to the darkening. The grain darkening was compared and evaluated by natural darkening method under shelf conditions, in days storage, and accelerated darkening method under ultraviolet light, in hours. The natural darkening time of 90 days was statistically equal to 24 hours of accelerated darkening, and the difference among the genotypes could be obtained in a shorter time, indicating a correspondence in the methods. The accelerated darkening method can be used to shorten the analysis time in the routine of breeding programs.

Research Article Genetic diversity as an additional selection criterion in a carioca common bean recurrent selection program for resistance to common bacterial blight

Common beans are a key source of protein and are consumed daily by most of the Brazilian population. More than 70% of what is consumed in this country is classified as carioca beans, based on seed qualities and appearance. We evaluated progenies of carioca common beans of the Brazilian agriculture research agency (Embrapa) recurrent selection program. This recurrent selection program is based on resistance to common bean bacterial blight (CBB), seed yield, and other important agronomic traits. Selection also considers the genetic representativeness of the parents and the genetic diversity among phenotypically selected progenies. Initially, 60 superior progenies were selected based on resistance to CBB and on seed type, in two locations (Santo Antonio de Goias, GO and Ponta Grossa, PR). These progenies were evaluated in the C0S0:2 generation in field trials in six locations during the rainy growing season. A randomized block experimental design was used with two replications, in plots of two 3-m rows spaced at 0.5 m. The most promising C0S0:3 progenies for combined agronomic performance in the different environments were selected and were then evaluated by means of 24 microsatellite molecular markers for the purpose of determining the genetic representativeness of their parents and the genetic diversity among them. The phenotypic data was subjected to analysis of variance for each trait in each of the environments, and then joint analyses were performed. To estimate genetic diversity among the progenies, the Rogers-W genetic distance was used, and a dissimilarity matrix was used to construct a dendrogram of genetic distances through the UPGMA method. Among the 60 C0S0:2 progenies that were evaluated, those that stood out for resistance to CBB also stood out for seed yield and for resistance to other diseases. Various progenies were superior, exhibiting large genetic distances between each other and in relation to the parents. This indicates a possibility of direct and indirect gains from the recurrent selection program. This molecular information will help direct selection of individuals for future recombination cycles.