Caries Development In Children Research Articles

Nutritional habits as risk factors for dental caries in children from rural and urban areas in Skopje

Proper nutrition is a long-term investment in one’s own health. Oral health as an integral part of general health is in bidirectional relationship with diet and nutrition. Globalization and urbanization have led to nutrition transition from a traditional agriculturally-based diet to consumption of processed foods that are high in sugar. Children have been particularly affected by this change. The aim of this study was to investigate the association between specific nutritional and hygiene habits and the development of caries in children from urban and rural areas. The study was conducted in December 2019 and comprised children aged 6 years. A survey was made and children’s dental status was tested. The results obtained showed a higher percentage of healthy deciduous (50%) and permanent (35%) teeth in children from rural areas than in children from urban area. Both groups had similar habits in terms of cariogenic and cariostatic food intake, but there were differences in children's hygiene habits.

Peculiarities of the state of the hard tissue of the temporary teeth in 6-years-old children

Literary data show that caries is the most common dental nosology in the world. After conducting epidemiological studies of our region, we established a high incidence of caries in children aged 6 according to WHO criteria. In this regard, we set ourselves the aim of assessing the peculiarities of the state of hard tissues of temporary teeth in children living in different regions of Bukovyna. To solve the aim, we examined 306 children in 13 schools of the Chernivtsi region. The following observation groups were selected: Group I – 93 children aged 6 years living in Vyzhnytskyi District, Group II – 119 children aged 6 years living in Chernivtsi District and Subgroup III – 94 children aged 6 years living in Dniester District. We analyzed the prevalence, intensity of caries of temporary teeth, level of intensity, ICDAS1-6 in different regions of Bukovyna. The degree of probability of the obtained results was statistically assessed. The prevalence of caries of temporary teeth in 6-year-old children is high in all regions: 93.54% in Vyzhnytskyi, 93.61% in Dnistrovskyi and 92.43% in Chernivtsi districts. The analysis of the intensity of caries showed that the highest values – in children of the Vyzhnytsky district (5.46±0.33) affected teeth, the lowest – in the examined children of the Dniester district – (5.05±0.28). The "d" component dominated the structure of the "dmf" index in all regions. It should be noted that the extracted teeth were from 1.59 to 4.39%. In all regions, the average level of intensity prevailed, but the share of children with a high level was also high: 39.78% in Vyzhnytskyi, 31.91% in Dniester and 30.25% in Chernivtsi districts. The lower second molar was most often affected, with a share of 26.53%. A combined lesion of several groups of teeth, in particular the upper and lower molars, as well as the upper frontal teeth that were present, is characteristic. Carious cavities were most often located on the masticatory (31.25%) and contact surfaces (51.52%). Enamel caries was most often diagnosed in 41.21% of cases, a third of all lesions (36.64%) were dentine caries, and a fifth part (22.13%) were caries at the stain stage. Therefore, the high incidence of caries in children in all districts of the Chernivtsi region indicates the need to study the regional risk factors for the development of caries in children for the further development of regionally adapted treatment and prevention programs. Keywords: children, caries, complications of caries, prevalence, intensity, SIC-index.

Role of mutans streptococci, acid tolerant bacteria and oral Candida species in predicting the onset of early childhood caries.

Early childhood caries is the most common chronic infectious disease in children in the United States. This study, which is part of a larger, longitudinal study exploring oral microbiological components of caries development in children, reports on the impact of total mutans streptococci (MS), total acid tolerant bacteria and Candida species on the development of dental caries in a subset of these children. Of particular interest was the relationship between caries development and co-colonization of mutans streptococci and Candida species. Children between the ages of 12 and 47 months displaying no evidence of dental caries were recruited for a longitudinal study (n = 130). Twelve age- and gender-matched pairs were selected. In each pair, one child developed caries during the study, and one did not. Whole mouth plaque samples were collected by swab at baseline and every 6 months thereafter for a duration of 18 months and spiral plated for microbial counts (CFU/ml). Cut-offs based on percent of total cultivable flora were designated for all microbial measures. A scoring system designated the Plaque Microbial Index (PMI) was developed for use in statistical analyses to assess potential predictive factors for caries risk assessment. Children who developed caries were significantly more likely to harbor higher percentages of acid tolerant bacteria (p = 0.003), MS (p < 0.001) and have Candida species present (p < 0.001) at ≥1 visit leading up to caries onset. Mean PMI scores derived from the aforementioned microbial measures, were higher for caries active children than caries free children (p = 0.000147). Co-colonization of MS and Candida species was significantly associated with caries development (p < 0.001) and detection of both at the same visit had a 100% positive predictive value and 60% negative predictive value for caries development. In children who developed caries, there was a statistically significant association with the percent of total flora that was acid tolerant, the percent of MS, the presence of Candida and co-colonization of MS and Candida species. Combining these microbial measures into PMI scores further delineated children who developed caries from those who remained caries-free. These microbiological measures show potential as predictive factors and risk assessment tools for caries development.

The relationship between socio-behavioural factors in family environment and dental caries development in children

Objective: the assessment of the relationship between socio–behavioural factors in family environment and dental caries in school age children. In the case–control study, 82 children between the ages of 11 and 14 were clinically examined. In the research batch (L1) were included 41 subjects with dental caries. The control group (Lo) consisted of 41 children without caries. There were studied dental caries indices dental caries experience. Socio–behavioural factors in the family environment were collected using the questionnaire method. The analysis of the data achieved was performed using parametric and non–parametric tests of Excel Software and Epi Info. In order to determine if there are associations between the studied variables, the Spearman correlation coefficient has been valued. There were positive, strong and very strong correlations between the indicators of dental caries damage and the following factors: the mother’s level of education (incomplete secondary education) ρ=0.86, p&lt;0.01, the parental check absence of oral hygiene (ρ=0.84, p&lt;0.001), the preventive examination refusal and dental treatment (ρ=0.79, p&lt;0.01). Conclusions: the establishment of socio–behavioural risk factors in the family environment is relevant to the creation of a health policy aimed at meeting the needs of the population, with the establishment of a hierarchy of care’s priority and evaluation of sources allocated for the implementation of oral hygiene programs, the achievement of dental diseases treatment and the implementation of preventive measures.

Acidulated phosphate fluoride sebagai bahan pencegahan Karies gigi anak : a scoping review (Laporan Penelitian)

Background: Caries occur in the hard tissues of the teeth due to bacterial activity in plaque. Untreated dental caries can result in pain, therefore early and if caries is left over time it will cause pain so that early caries prevention is necessary. Caries prevention can be done using Acidulated Phosphate Fluoride as an effective option in preventing the process of caries development in children. Aim: To know the effectiveness of APF gel 1,23% in preventing caries in children. Method: Scoping Review with inclusion criteria using a Randomized Controlled Trial (RCT) study design was conducted according to Preferres Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and two databases (PubMed and Google Scholar). Result: The results showed that the initial search yielded 985 documents. After eliminating duplicate studies and screening full-text articles for eligibility (n = 21), the authors identified 3 studies that met the criteria for inclusion. Conclusion: APF gel 1.23% has the effectiveness and preventing dental caries in children due to its better ability to bind calcium enamel,also does not irritate the gingiva..

Features of the course of teeth caries in children of Bukovina region

The prevalence of dental diseases is high, regardless of age. In the literature, the indicators of the prevalence and intensity of the main dental nosologies in different parts of Ukraine are widely covered. However, they are significantly higher in regions with contrasting geographical conditions, one of which is the Chernivtsi region. That is why the study of dental morbidity in children in the age aspect in this region is relevant. The purpose of the study is to study the prevalence and intensity of caries of temporary and permanent teeth in children of different ages living in Bukovina. To establish the dental status of children in Bukovyna, we examined 839 children of various ages in 13 schools of Chernivtsi region. The following observation groups were selected: I – 306 6-year-old children, II – 298 12-year-old children, III – 235 15-year-old children. The prevalence, intensity of caries of temporary and permanent teeth, Significant Caries Index, level of dental care were evaluated. The degree of probability of the obtained results was statistically assessed. Because of the conducted research, it was established that the incidence of caries of temporary and permanent teeth, regardless of age and region of residence, is high: in 6-year-old children ‒ 96.73%, in 12-year-old children ‒ 84.89%, in 15-year-old children ‒ 89.78%. In temporary teeth, the intensity of caries was 5.19±0.30 teeth. In the structure of the index, the "d" component was from 77.47% to 87.45% depending on the region, with the share of caries complications in this structure – 12.53‒15.48%. Prematurely removed temporary molars were detected in all regions of Bukovyna: from 1.59% to 4.39%. Every fourth to fifth child aged 6 has 1-2 recently erupted permanent teeth affected by caries, and in mountainous areas – almost every second one. The "D" component was also predominant. In its structure, 5.55% were complications of caries in recently erupted permanent teeth of children living in the mountainous region. With age, the values of the caries intensity index increased: 4.28 times in 12-year-olds and 6.40 times in 15-year-olds compared to 6-year-olds. The ratio between the components of the indicator remained the same as in the previous age period ‒ the number of carious teeth predominated over filled ones. At 12-year-old children, caries complications were 7.95%, at 15-year-old children – 5.57%. The level of providing dental care to children was satisfactory only for 15-year-old children of the Vyzhnytsky district. In the rest of the regions, regardless of age, the level of providing dental care was insufficient. Therefore, the high prevalence and intensity of caries of temporary and permanent teeth, the insufficient level of providing dental care indicate the need to study the risk factors for the development of caries in children for the further development of regionally adapted treatment and prevention programs. Keywords: children, caries, complications of caries, prevalence, intensity, SiC-index.

The use of anti-asthmatic inhalation therapy and the risk of dental caries in a group of Polish children: a prospective study.

Assessment of the effect of inhaled therapy with anti-asthmatic medications on the development of caries in children between 3 and 17 years old. Study design: The study involved 208 patients, 114 in the study group and 94 in the control group. Data on general health condition of patients, use of medications, dietary habits and oral hygiene were obtained. Information on the onset of asthma, its severity and type, doses and method of administration of asthma medications were obtained from the available medical records by the paediatrician. Dental examination was performed using the DMFT/dmft and DMFS/dmfs index, ICDAS II classification and Nyvad's criteria for noncavitated white spot lesion differentiation. Additionally, an assessment of salivary S. mutans (SM) and Lactobacillus spp. (LA) was carried out using the CRT Bacteria (Ivoclar Vivadent) test. There is still no clear consensus among the authors as to whether asthma and medications used in its treatment increase the risk of caries. Nowadays, despite better access to various sources of information, there is still a need for increased awareness in patients and medical doctors on the dental care and caries prevention in patients with asthma.

Assessment of dental caries in children with organic lesions of the nervous system using ICDAS II criteria.

Studies of the dental status of children with cerebral palsy (CP) indicate a high prevalence and intensity of damage to the hard tissues of the teeth. The risk of developing dental diseases is known to increase significantly as the severity of neurological symptoms increase. The purpose of the study was to assess the incidence of dental caries using the International Caries Detection and Assessment System (ICDAS II) criteria in children with organic diseases of the nervous system depending on the severity of motor impairment. A number of 122 children (mean age 8.8±3.7 years) with spastic forms of cerebral palsy were examined. They were divided into groups according to the Gross Motor Function Classification System – Expanded & Revised (GMFCS-ER). All patients underwent a neurological examination, and the state of dental caries was determined using the ICDAS II criteria. In children with cerebral palsy, lesions of the occlusal surfaces of the teeth predominate, lesions of the proximal surfaces appeared to be three times less, but more than three times higher than in healthy children. Higher intensity of the carious process and the frequency of deep cavities are observed in children with cerebral palsy with severe motor impairment, according to GMFCS-ER. Establishing the features of caries development in children with cerebral palsy depending on the severity of neurological symptoms according to the ICDAS II system is an essential factor in determining the direction of preventive measures that should be taken for this group of children.

Association between demarcated enamel hypomineralization on second primary molars and dental caries in childhood

Hypomineralized Second Primary Molars (HSPM) is the name used to describe the presence of demarcated enamel hypomineralization in second primary molars. HSPM has been compared with Molar Incisor Hypomineralization (MIH) as regards its clinical appearance and consequences. The aim of this study was to investigate associations between HSPM and dental caries in childhood. It was a cross-sectional populationbased study that included 216 children aged 4-6 years from public schools in Botelhos, Brazil. Children with all second primary molars erupted were eligible for participation. Clinical examination was performed by one calibrated examiner. HSPM was recorded according to modified European Academy of Paediatric Dentistry criteria. The main outcome was dental caries experience as indicated by the dmft index (%dmft&gt;0) according to WHO criteria. Chi-square test and Logistic Regression Model were used to adjust the results for effects of covariates Among the 216 children examined, 22.2% presented HSPM. After adjustment for logistic regression, children with HSPM were found to have a 2.28 times greater chance of presenting dental caries. Our results reinforce the importance of HSPM in caries development in children, which should be considered by public health dentists in the process of planning actions aimed at this population.

ВОЗМОЖНОСТЬ ПРОГНОЗИРОВАНИЯ КАРИЕСА ЗУБОВ У ДЕТЕЙ В ПЕРИОД СМЕННОГО ПРИКУСА

Background. Preventive measures that are implemented through the introduction of regional prevention programs have a massive focus and do not lead to a decrease in dental morbidity, because they are based on routine preventive measures without taking into account the individual characteristics of metabolic processes in the patient's oral cavity. All this confirms the need to abandon the mass approach when carrying out preventive measures, and requires the search for modern methods for predicting dental caries, based on predictive diagnostics of the risk of caries, which should be a key element of clinical examination of the pediatric population by a dentist.&#x0D; Purpose of the study: on the basis of a comprehensive clinical and laboratory approach with the use of mathematical analysis of the obtained data on homeostasis of the oral cavity, to establish predictors of the development of the carious process in children in order to determine the possibility of predicting dental caries during the period of mixed bite.&#x0D; Methodology. To achieve this goal, a comprehensive clinical and laboratory examination of metabolic processes in the oral cavity was carried out in 60 caries-resistant children during the period of mixed bite: from 7 to 12 years. Statistical analysis was performed using the STATISTICA 8.0 software.&#x0D; Results. According to the results of the study, strong relationships were revealed between individual clinical and laboratory indicators-predictors, an optimal set of predictors was established for constructing classification models for patients with different types of MCS, which is a prerequisite for the possibility of predicting the risk of caries development in children during the mixed bite period.&#x0D; Conclusions. An optimal set of predictors has been established for constructing classification models for patients with different types of ISS for creating computer programs aimed at preclinical diagnostics of the subclinical course of the carious process with access to prediction, which will allow planning individual primary preventive measures in children during the period of active formation of hard dental tissues.

Effectiveness of dental caries prevention in children with bronchial asthma

Asthma is one of the common diseases in the pediatric population in children, the prevalence of asthma in children in the world is 5 – 10 %. According to the literature, the dental caries in children with asthma is 68. 45 – 88. 1%. The development of dental caries in children with asthma is affected by changes, which are in the body of children, as well as long-term drug treatment.Objective. To evaluate the effectiveness of preventive measures in children with asthma.Material and methods. Under our supervision, which lasted for two years, there were 144 12-year-old children suffered from asthma and were treated in the pulmonary allergy department of the children's regional hospital. A set of measures was developed for the prevention of dental caries for children with I-II and III-IV severity of asthma. Assessment of the effectiveness of the complex of preventive measures was carried out after 6, 12, 24 months according to the following criteria: caries intensity (DMF), reduction of the increase in the dental caries intensity, enamel resistance by TER.Results. The improvement of the following indicators was found for 2 years of our observation: decrease in the increase of caries intensity in children with III-IV compared with I-II severity of asthma; improving of oral hygiene (according to the Green-Vermillion index); increase of enamel resistance by TER test. The reduction of the increase in caries intensity in children with asthma constitutes 46.94% in children with I-II and 51.02% with III-IV severity of asthma.Conclusions. The analysis of the results of the children’s examination for two years of application of the developed set of measures for dental caries prevention shows that the implemented measures are effective for use in children depending on the severity of asthma.

Do enamel and dentine caries at 5 years of age predict caries development in newly erupted teeth? A prospective longitudinal study

Objective: To explore caries development in children from 5 to 12 years of age, and to study whether enamel caries and dentine caries at 5 years of age could predict caries prevalence at 12 years of age, controlled for child characteristics.Methods: The study included 3282 children examined at 5 and 12 years of age. Data were collected by clinical examination and questionnaire. Enamel and dentine caries were registered at surface level. Data were tested by t-test and analysed by bi- and multivariate logistic regression. The study was ethically approved.Results: In 5-year-olds, 15% of the children had dentine caries experience and 21% had enamel caries. In 12-year-olds, 32% had dentine caries experience and 47% had enamel caries. Children with dentine caries experience at 5 years of age had at 12 years of age developed more surfaces with enamel caries (mean 2.8, SD 4.2) and dentine caries experience (mean 1.8, SD 2.5) than other children (p < .05). Dentine caries experience at 12 years of age was associated with having only enamel caries (OR 1.6, CI 1.2–2.0) and dentine caries experience (OR 3.2, CI 2.6–3.9) at 5 years of age. Family status and parental education were related to caries development.Conclusion: Children with caries in primary teeth continued to be caries risk children during the mixed dentition period. In addition to dentine caries experience, enamel caries in primary teeth was a predictor for caries development in young permanent teeth and may be used to improve the caries risk assessment.

Birth order is associated with caries development in young children: a register-based cohort study

BackgroundBirth order has been shown to affect the health of the child; less is known, however, about how birth order affects caries development in children. Thus, the present study investigated the association between birth order and dental caries development in young children.MethodsThis retrospective registry-based cohort study included all children born in 2000–2003 who were residing in Stockholm County, Sweden, at age 3 years (n = 83,147). The study followed the cohort until subjects reached 7 years of age. Children with registry data on dental examinations and sociodemographic characteristics at ages 3- and 7 years constituted the final study cohort (n = 65,259). The outcome variable was “caries increment from age 3- to 7 years” (Δdeft > 0) and the key exposure, “birth order”, was divided into five groups. A forward stepwise logistic binary regression was done for the multivariate analysis with adjustments for sociodemographic factors.ResultsAt age 3 years, 94% had no fillings or manifest caries lesions. During the study period, 22.5% (n = 14,711) developed dental caries. The final logistic regression analysis found a statistically significant positive association between birth order and caries increment. Further, excess risk increased with higher birth order; with the mother’s first-born child as reference, risk for the second-born child was OR 1.17, 95% CI = 1.12–1.23; for the third-born child, OR 1.47, 95% CI = 1.38–1.56; for the fourth-born child, OR 1.69, 95% CI = 1.52–1.88; and for the fifth-born or higher birth-order child, OR 1.84, 95% CI = 1.58–2.14.ConclusionsThese findings show that birth order influences caries development in siblings, suggesting that birth order can be regarded as a predictor for caries development in young children. This factor may be helpful in assessing caries risk in preschool children and should be considered in caries prevention work in young children with older siblings.

POSSIBILITIES OF CARIES PROGNOSIS IN CHILDREN OF SCHOOL-AGE ACCORDING TO DATA GAINED FROM THEM AND THEIR PARENTS QUESTIONNAIRE

Caries in children is one of the main dental problems that scientists are trying to solve [1, 2, 3]. A considerable number of methods of treatment and prevention of caries have been developed. However, the issue remains unresolved [4, 5]. Much efforts are devoted to the identification of general and local risk factors of caries appearance in children and the possibility of influence on them [6, 7]. Questionnaire is one of the forms of determining caries appearance factors [8]. This method of research allows to identify and compare the significance of factors observed in children and their parents. Therefore, the purpose of our study was to identify the possibility of predicting caries in preschool children according to questionnaires of surveyed children and their parents.&#x0D; We performed survey and questionnaire of 223 children aged from 7 to 12 years. A questionnaire was also offered to the parents of these children. The examination of children was carried out in children’s organized collectives of Poltava. In all children the caries intensity was determined according to the following indices of df, DEF and DEF + def.&#x0D; The quantitative indices obtained during the examination of patients were processed with standard methods of mathematical statistics. For the semi-quantitative and qualitative indices, the frequency tables were built. Since most of the indicators were semi-quantitative and qualitative, Spirman’s coefficient of pair correlation was determined for the analysis of the relationships of the studied parameters. The correlation coefficient was considered significant in the case of of error probability “p &lt;0,05”. Discriminant analysis was conducted in order to determine the factors of classification. Numerous dependencies were observed between the revealed behavior features, habits, peculiarities of parents’ and children’s views concerning dental health surveyed and dental indices of the examined children.&#x0D; Thus, the need for preventive examinations was documented by 87.5% of parents and 92.9% of children without caries and only 63.0% of parents and 74.1% of children with the highest degree of caries affection. The proportion of children who consumed carbonated beverages grew with the development of carious process. Anxiety was also noted more often in children with the high degree caries affection.&#x0D; The received algorithms for analyzing the factors of caries development and the calculation of probability of child assignment to the each of four groups of caries affection provide an opportunity for preventive work with parents and children. According to the questionnaires of parents, it should be noted that there is a frequent underestimation of caries progression in comparison with the algorithms that consider the results of questionnaires of children, particularly, the group of children with low activity of carious process (almost 40% of children with caries). Therefore, it's better to consider data gained from children, rather than stay restrained parental questionnaire.&#x0D; The performed research allowed to identify a group of factors that affect the appearance and development of caries in children. Among these factors (according to the questionnaires of children) the most important are: the amount of complaints about the state of hard tooth tissues, swell of gingiva, the number of toothbrush characteristics considered during its selection (the most important reducing risk factor), circles attending (the highest risk factor), consummation of carbonated beverages (the second most significant risk factor).&#x0D; There are factors with the biggest impact on the appearance and development of caries in children (according to parents questionnaires): income; preventive examinations (the most important reducing risk factor); flossing (an important risk factor); parents' assistance during tooth brushing; toothpaste choice depending on the mechanism of their action; toothbrush choice considering its size and toothbrush head shape (the biggest risk factor); visits of sports sections by parents.&#x0D; Prospects for further research. The obtained results lead to a more detailed study of peculiarities of the revealed factors influence in the development of caries in school-age children. This will be the next aim for the further research.

CORRELATION RELATIONS OF CARISES AND SOCIO-HYGIENIC FACTORS

There is a demand in statistical processing of questionnaire data and objective screening of children with the usage of correlation analysis as well as interview with parents in order to increase their awareness about caries prevention and lifestyle. The emergence of caries in children 7-12 years of age is influenced by socio-hygienic factors. During the interview with parents, the effect of a combination of factors was found (i.e. avoiding referring to a dentist for the purpose of prevention or professional hygiene, neglecting the additional hygienic tools and methods and low awareness about them, regularity and balance of nutrition, lack of awareness concerning etiology of caries appearance, referring to a dentist only in case of toothache, anxiety during a visit to a dentist. Interview with children showed the impact of a combination of factors on the occurrence of caries. Among them are evasion of referring to a dentist for the purpose of prevention, lack of awareness concerning etiology of caries appearance, referring to a dentist only in case of toothache, anxiety during a visit to a dentist, low awareness concerning additional hygienic tools and methods. The combined effect of parental awareness and habits on the development of caries in children is much higher than the effect of the respective peculiarities of children. Thus, the analysis of caries appearance factors in children aged 7-12 years reveals the existence of close relationships between the alimentary, behavioral habits of parents and the appropriate habits of children and the development of pathological changes in the oral cavity of children which leads to the propagation of carious lesions. This requires improvement of preventive work with parents aimed at formation of healthy lifestyle habits and oral health care in order to prevent pathology of oral cavity and masticatory apparatus.

Взаємозв’язок між інтенсивністю карієсу, структурно-функціональною резистентністю емалі та гігієною порожнини рота у дітей із карієсом оклюзійної поверхні жувальної групи зубів

Summary. The article describes the relationship between caries intensity, oral hygiene and enamel resistance during the period of secondary mineralization of teeth is an actual task, the solution of which will be the basis for the justification of caries prevention.The aim of the study – to assess the relationship between caries intensity, oral hygiene and enamel resistance during the period of secondary mineralization of the teeth.Materials and Methods. 156 children were examined, among them 76 were between 12 and 15 years of age and 80 children aged 16 to 18 years. To assess the odor of caries of chewing teeth, the intensity of the KPV index and intensity levels according criteria were determined. The condition of hygiene of the oral cavity was estimated using the hygienic index Green-Vermilion. Structural-functional resistance was determined by the TER-test Okushko V. R., Kosareva L. I. (1983).Results and Discussion. As a result of our research, we found that with an increase in the intensity of the carious process in children of both groups of the study deterioration of the hygienic state of the oral cavity according to the OHI-S index. Also between this index and TER-test there is a strong correlation relationship. When TER-test increases the value of OHI-S index enlarges.Conclusions. Unsatisfactory level of hygiene of the oral cavity, especially during the period of active mineralization of the enamel, contributes to reducing the resistance of the enamel, which plays a significant role in the development of caries in children of the study group.

Association of Salivary Microbiota with Dental Caries Incidence with Dentine Involvement after 4 Years.

Salivary microbiota alterations can correlate with dental caries development in children, and mechanisms mediating this association need to be studied in further detail. Our study explored salivary microbiota shifts in children and their association with the incidence of dental caries with dentine involvement. Salivary samples were collected from children with caries and their subsequently matched caries-free controls before and after caries development. The microbiota was analyzed by 16S rRNA gene-based high-throughput sequencing. The salivary microbiota was more diverse in caries-free subjects than in those with dental caries with dentine involvement (DC). Although both groups exhibited similar shifts in microbiota composition, an association with caries was found by function prediction. Analysis of potential microbiome functions revealed that Granulicatella, Streptococcus, Bulleidia, and Staphylococcus in the DC group could be associated with the bacterial invasion of epithelial cells, phosphotransferase system, and D-alanine metabolism, whereas Neisseria, Lautropia, and Leptotrichia in caries-free subjects could be associated with bacterial motility protein genes, linoleic acid metabolism, and flavonoid biosynthesis, suggesting that functional differences in the salivary microbiota may be associated with caries formation. These results expand the current understanding of the functional significance of the salivary microbiome in caries development, and may facilitate the identification of novel biomarkers and treatment targets.

Risk factors for caries-free time: longitudinal study in early childhood.

ABSTRACTOBJECTIVE To estimate time in days from the beginning of follow-up up to the development of dental caries in children under 30 months and to assess risk factors potentially affecting the development of the disease.METHODS The study population of the cohort study were children attending public, private, and charitable day care centers in Salvador, Northeastern Brazil, followed up for fourteen months. We used the multivariate Cox regression to estimate risk and Kaplan-Meier method to estimate the caries-free time.RESULTS Of the 495 children studied, 112 developed caries (22.6%). Mean caries-free time was 248.6 (SD = 96.2) days. The comparasion of curves by age group (> 24 months) and children attending public day care showed more caries in a shorter period (p < 0.00). The following variables were important risk factors for increased rate of caries: district of origin (HR = 1.88, 95%CI 1.27–2.77), category of day care (HR = 3.88, 95%CI 2.04–7.38), age (HR = 1.77, 95%CI 1.15–2.74), bottle-feeding before sleep time after the age of 12 months (HR = 1.62, 95%CI 1.04–2.51), presence of active white spots (HR = 2.70, 95%CI 1.07–6.80), and living in non-masonry house (HR = 1.68, 95%CI 1.02–2.76). The highest hazard ratio (HR = 4.60, 95%CI 2.80–7.42) was found for previous caries experience.CONCLUSIONS Social variables were considered as of high risk for the development of dental caries.

Factors associated with the risk of caries development after comprehensive dental rehabilitation under general anesthesia

Background/purposeThe aims of this cross-sectional study were to determine the possible risk factors of caries development in children with early childhood caries (ECC) and to perform a caries risk assessment using Cariogram over a 12-month period. There is no report in examining caries risk assessment under 6 years old children in Taiwan. Materials and methodsSeventy-nine children (mean age 48.80 ± 10.71 months) with ECC indicated for comprehensive dental reconstruction under general anesthesia were selected. A questionnaire was completed by the parents to assess the possible caries-related factors at a 12-month follow-up. Data were collected for caries index, plaque index, Streptococcus mutans (SM) and Lactobacillus (LB) counts, and salivary buffering capacity at the initial examination and at the 12-month follow-up. Children were divided into two groups: caries-free (decayed, extracted, and filled teeth = 0) and caries-recurrent (decayed, extracted, and filled teeth > 0). The children's caries risk was assessed using the Cariogram software program. ResultsAt the 12-month follow-up, 79.7% of the children had developed new caries. No significant differences were found in parents' education levels, child's oral health practices, or parental knowledge and attitudes toward oral health (P > 0.05) between the caries-free group and the caries-recurrent group. The SM count (P = 0.001) and caries risk assessment (CRA) score (P < 0.001) were found to be significantly associated with new caries development. ConclusionThis study has shown that SM count and CRA score were associated with new caries development in ECC children who needed to be treated under general anesthesia. The modified Cariogram used in this study is another significant tool for predicting new carries development in this particular population.

Perinatal outcomes and changes in the oral cavity: Brazilian cohorts of Ribeirão Preto and São Luís.

Studies have shown a possible association of oral diseases during pregnancy with preterm birth (PTB) and low birth weight (LBW). These perinatal outcomes appear to be associated with enamel defects in the primary dentition, which, in turn, seem to predispose to future development of caries in children. Therefore it is relevant to include oral health variables of the mother/child dyad in cohort studies to understand how these factors are associated. The objectives of this study are: 1) check if there is an association between diseases of the oral cavity of pregnant women and PTB, 2) test the hypothesis of association between perinatal outcomes and enamel defects/dental caries in children, 3) examine whether there are associations between perinatal outcomes and disorders of tooth eruption in children; 4) build theoretical models to study social inequities as a common factor between oral conditions and perinatal outcomes. We used an integrated, collaborative approach between two Brazilian cities with contrasting socioeconomic conditions: Sao Luis , MA, and Ribeirão Preto, SP - British Birth Cohort Studies study (BRISA Ribeirão Preto, São Luís). Two cohorts were evaluated: one initiated at birth, representative of the population of live births, and another, initiated prenatally. Participants were reassessed from the beginning of the second year of life. It is expected that these cohorts will contribute to foster the development and consolidation of population-based follow-up studies in Brazil.