Introduction: Unfamiliarity with crash cart contents contributes to decreased patient safety. There is no standardization of crash carts organization or contents. Initial moments of an emergency are critical and especially stressful. Responders familiar with the available equipment may be more efficient in retrieval and utilization of supplies. Methods: This study investigated the effects of Rapid Cycle Deliberate Practice (RCDP) as a training tool to increase familiarity with crash carts and contents, through a challenge of timed retrieval and assembly of critical items needed in response to in-hospital cardiac arrest (IHCA). Individuals were randomly assigned to one of four experimental groups to further identify efficient education methods. Hypothesis: RCDP predicted to improve retrieval performance and participant group assigned to hands-on discovery of the crash cart would show most improvement. Results: 75 interdisciplinary participants averaged total time for retrieval of 12 critical care items with completion of 4 corresponding tasks of 327 seconds decreased to 104 seconds from round 1 to 2. Secondly, average number of drawer pulls to retrieve items went from 14.8 to 10.5. The item most infrequently found in the 60 seconds allotted in round 1 was the intraosseous access kit (14/75, 18.67%). Connection of the defibrillator electrodes to the defibrillator device failed completion by 23 participants (30.67%) in the 60-second round 1. Retrieval of intraosseous access kit and connection of defibrillator electrodes was successful by all participants in round 2. Conclusions: Findings highlight the importance of training staff on available emergency equipment, with the expectation that increased familiarity and decreased response times will correlate to improved patient IHCA outcomes. Furthermore, findings indicate a need for standardization of crash cart organization across units and facilities.