Carcass Fatty Acid Composition Research Articles

An investigation of slaughter weight and muscle type effects on carcass fatty acid profiles and meat textural characteristics of young Holstein Friesian bulls

Study objectives included the assessment of carcass fatty acid composition and meat texture characteristics of younger Holstein Friesian bulls. Three experimental groups were formed based on the weights of the 23 young bulls at slaughter: lighter, medium, and heavier. Samples were taken from the Gluteus medius (GM) and Longissimus thoracis muscles 24 h after slaughter. Fatty acid composition, Warner-Bratzler Meat Shear (WBS) measurements, as well as textural profile analysis (TPA) and sensory analysis of the muscle samples were conducted. The fatty acid composition was determined using Thin Layer Chromatography (HPTLC). Polyunsaturated fatty acids and dietary fatty acids give a neutral hypocholesterolemic effect in direct fluorescent antibody (DFA) contents, DFA/OFA (C14:0+C16:0) ratio, hardness, Warner-Bratzler Shear force and also the chews number – which is desirable - before swallowing (NCBS) the meat were significantly decreased with the increasing slaughter weight. Higher slaughter weight resulted in a larger amount of beef with a better panel tenderness score; however, the meat obtained from the LSW group was less healthy considering the fatty acid profile. Additionally, internal fat contained the highest saturated fatty acids concentrations, while subcutaneous fat contained the highest amount of monounsaturated fatty acids. Furthermore, intramuscular fat levels were highest in PUFA and PUFA/SFA ratio. As a result, this study strongly suggests that slaughter weight and anatomical location of fat samples contribute significantly to meat texture characteristics and fatty acid profiles in Holstein Friesian bulls.

Effect of feed enriched with fermented coconut water products on growth and carcass composition of gurami sago (Osphronemus goramy Lacepède, 1801) juvenile.

Background Giant gourami (Osphronemus goramy Lacepede, 1801) is a popular freshwater species in Indonesia, but there is limited information on feed used for its cultivation. Therefore, this study aims to determine effect of feed enriched with fermented coconut water and palm sap sugar solution on growth, feed efficiency, and carcass composition of juvenile sago gurami. Methods A total of 2 litres coconut water and 1litres palm sap sugar solution (10%) were formulated. Each formulated product was then mixed with 6 g Aspergillus niger (P1), 6 g Rhizopus oligosporus (P2), and 6 g Saccharomyces cerevisiae (P3) to obtain the supplements. Subsequently, supplementation was carried out on commercial feed at a dose of 300 ml/kg of feed. Samples supplemented with P1, P2, and P3 were designated P1, P2, and P3 diets, while feed added to freshwater was considered P4 (placebo). Gurami sago juvenile (initial weight 50±2.5 g and length 13.2±0.4 cm) were then placed in triplicate nets (2×1×1 m) in a freshwater concrete pond with a stocking density of 30 fish/net. Results The different products formulations had a significant effect (p<0.05) on growth performance. The weight gain in P1, P2, P3, and P4 diets were 167.24%, 193.99%, 134,22%, and 115.98%, respectively. For feed conversion efficiency, the values obtained were 0.65, 0.73, 0.65, and 0.64 in P1, P2, P3, and P4, respectively. Furthermore, supplementing commercial feed with varying products formulation had a significant impact (p<0.05) on the fatty acid composition and carcass body of gurami sago. Lipid content in fish carcass fed P1, P2, P3, and P4 were 2.90%, 4.42%, 2.98%, and 2.76%, respectively. Conclusion Based on the results, P2 contained a higher concentration of fatty acids compared to other diets, leading to increased body weight, feed efficiency, and carcass fatty acid composition in sago gurami reared in freshwater concrete ponds.

A matter of body weight and sex type: Pig carcass chemical composition and pork quality

This study compares minced carcass chemical composition and meat quality of castrated (CM), immunocastrated (IM) and entire male (EM), and female (FE) pigs at 70, 100 and 120 kg target body weights (TBW) (n = 80; 20 per sex). Sex affected fat, protein, and moisture content of the minced carcasses. Carcass fatty acid (FA) composition was affected by sex, with higher saturated and monounsaturated FA content in CM than in FE, IM and EM, and higher polyunsaturated FA in CM than in EM, with FE and IM in between. Except for intramuscular fat, which was higher in CM than in FE and EM, no significant differences between sexes were found in meat quality. TBW affected carcass chemical composition and some meat quality traits. An interaction between sex and TBW was found with IM approaching EM or CM depending on TBW.

Carcass fatty acid composition and sensory properties of Nile tilapia (Oreochromis niloticus) fed on oilseed meals with crude papain enzyme

In this study, proximate body composition, fillets fatty acid and sensory properties of Nile tilapia fed on oilseed meals with crude papain enzyme were determined. A control diet (D1) of 300g/kg crude protein (CP) and (2900Kcal/kg) was formulated using fishmeal (Rastrionaebola argentea) (FM) and test diets by replacing 10% CP of FM by soybean meal (SBM) (D2), canola meal (CM) (D3) and sunflower meal (SFM) (D4). One hundred and one days feeding trial was conducted in a 4x2 factorial design on 4 diets (D1, D2, D3 and D4) with (0.06%) and (0%) enzyme using 720 Nile tilapia fingerlings (7±3g). Fish were fed twice daily at 5% of their biomass at 10am and 4pm in two equal meals. At the end of feeding trial, fish were starved for 24 hrs and weighed. A sample of ten fish representing the average weight of each replicated group was used. The fillets fatty acid profiles were determined by MPA FT-NIR spectrometer. Organoleptic tests of both fresh and steamed fish were carried out by eight semi trained panellists using 5-point hedonic scale. There was increase (p<0.05) in carcass proximate composition in all the treatments. Fillets fatty acid levels were influenced by the crude papain enzyme (p<0.05). Palmitic acid (C16:0), linoleic acid (C18:2n-6) and oleic acid (C18:1n-9) were the most abundant fatty acids in the fillets. Fish fed on fishmeal based diet recorded higher percentage (22.56) of polyunsaturated fatty acids (PUFA). There was significant difference among the treatments (p<0.05) for general appearance. Fish fed on crude papain enzyme treated diets were less preferred. It is recommended that more research be done on the effect of papain enzyme on the carcass fatty acid and sensory properties of farmed fish.

The utilization of new products formulated from water coconut, palm sap sugar, and fungus to increase nutritional feed quality, feed efficiency, growth, and carcass of gurami sago (Osphronemus goramy Lacepède, 1801) juvenile

Background: Giant gourami (Osphronemus goramy Lacepede, 1801) has become popular aquaculture in Indonesia. However, information on the feed used is minimal. This study analyzed the change in feeding nutrition, fish growth, feed efficiency, and body-carcass using product formulated from water coconut, palm sap sugar, and fungus. Methods: A total of 2,000 ml coconut water and 1,000 ml of palm sap sugar solution were formulated. Each product formulated was added with 6 g of Aspergillus niger (called product P1), 6 g of Rhizopus oligosporus (called product P2), and 6 g of Saccharomyces cerevisiae (called product P3). Commercial feeds supplemented with P1, P2, and P3 products are designated P1, P2, and P3 diets. Commercial feed added to freshwater is called the P4 diet (placebo). Their dosage is 300 ml/kg of feed. Gurami sago juveniles (initial weight 50±2.5 g and length 13.2±0.4 cm) were stocked in triplicate nets (2×1×1 m) in a freshwater concrete pond with a stocking density of 30 fish/net, an initial feeding rate of 3% per day until study termination. Results: Our results support our hypothesis that different product formulations have a significant effect (p<0.05) on growth performance and feed efficiency. At the same time, supplementing commercial feed with varying formula products has a significant impact (p<0.05) on the fatty acid composition of the diets and carcass body of gurami sago. Conclusion: Diet P2 contains a higher concentration of fatty acids to increase body weight, feed efficiency, and the best carcass fatty acid composition than other experiments for sago gurami reared in freshwater concrete ponds.

Effects of switching from corn distillers dried grains with solubles- to corn- and soybean meal-based diets on finishing pig performance, carcass characteristics, and carcass fatty acid composition.

Corn distillers dried grains with solubles (DDGS) are known to negatively impact carcass yield and fat quality, thus finishing pigs may need to be switched from diets containing DDGS to corn–soybean meal (CSBM) diets before marketing (DDGS withdrawal). A total of 860 finishing pigs (PIC C48 or L42 × 327; initially 66.2 kg body weight, BW) were used in a 76-day experiment to evaluate the effects of DDGS withdrawal periods at increasing intervals before harvest. Pen served as the experimental unit, and there were seven replicate pens per treatment with 23 to 25 pigs per pen. Pens were blocked by BW and allotted to one of five dietary treatments differentiated by the DDGS withdrawal period: 76, 42, 27, 15, or 0 day before harvest. Diets contained 40% DDGS from 22 to 66 kg prior to the experiment, 0% or 35% DDGS during the experiment from ~66 to 82 kg, and 0% or 30% DDGS until the completion of the trial. Diets were not balanced for net energy. Linear and quadratic response to time following dietary switch was evaluated using PROC GLIMMIX. For the overall period (day 76 prior to market to day 0), as withdrawal period increased, average daily gain (ADG) and final BW increased (linear, P < 0.002) and feed efficiency (G:F) improved (quadratic, P = 0.019). Average daily feed intake increased (quadratic, P = 0.030) as withdrawal period increased. There was an increase (linear P = 0.010) in hot carcass weight (HCW), with a marginally significant increase in carcass yield (linear, P = 0.094) with increasing withdrawal period. Loin depth and lean percentage did not demonstrate any evidence for treatment differences (P > 0.132). Backfat increased (linear, P = 0.030) with increasing withdrawal period. Finally, iodine value (IV) of belly fat was decreased (linear, P = 0.001) with increased withdrawal period. In conclusion, switching from a DDGS-based diet to a CSBM-based diet for longer periods before slaughter increased ADG and improved G:F, resulting in increased HCW. After diets were switched from DDGS to CSBM, pigs demonstrated an increase in intake, likely due to the ability to consume high volumes of feed after consuming high fiber (DDGS) diets. Belly fat IV was decreased as the length of time after dietary change was increased, with the lowest IV resulting from pigs that consumed CSBM for the entire experimental period.

Feeding Whole Thraustochytrid Biomass to Cultured Atlantic Salmon (Salmo salar) Fingerlings: Culture Performance and Fatty Acid Incorporation

Replacement of fish oil by 5% thraustochytrid whole cell biomass in diets for Atlantic salmon had no ill effect on fish growth performance, carcass total lipid and total fatty acid content. Carcass fatty acid composition indicated incorporation of the dietary thraustochytrid-derived fatty acids. This was confirmed by compound specific stable isotope analysis (CSIA) which revealed significantly 13C-depleted (δ13C value of −24‰) ω3 long-chain (≥C20) polyunsaturated fatty acids (ω3 LC-PUFAs) in the fingerlings fed the thraustochytrid biomass containing diet, reflecting the highly 13C-depleted glycerol used to grow the thraustochytrid cultures. This finding demonstrates the bioavailability of the ω3 LC-PUFA of the Australian strain thraustochytrid culture (TC) 20 from the whole cell biomass that was partly cultivated on crude glycerol produced during biodiesel manufacturing. This paper demonstrates the value of Australian thraustochytrid strains grown heterotrophically for their wider biotechnological potential including as a source of higher value lipids, in particular the health-benefitting ω3 LC-PUFA, for use in aquaculture and other applications.

The technological meat quality of the White Mangalitsa breed

The technological meat quality of the White Mangalitsa breed

Effect of partial replacement of marine protein and oil sources in presence of lyso-lecithin in the diet of tiger shrimp Penaeus monodon Fabricius, 1978

&lt;p&gt;A 42-days growth trail was performed to evaluate the effect of lyso-lecithin in &lt;em&gt;Penaeus monodon&lt;/em&gt; (5.08±0.08 g) in terms of growth and carcass fatty acid composition. Three iso-nitrogenous and iso-lipidic diets were prepared by partially replacing both marine protein and oil sources using plant based ingredients in the presence of soy-lecithin or lyso-lecithin. Each experimental diet was randomly allotted to three tanks with fifteen shrimps per tank and all the tanks were equipped with the flow-through system. Results revealed that shrimp fed diet with lyso-lecithin had significantly (p&amp;lt;0.05) higher specific growth rate, SGR (2.15) and daily growth coefficient, DGC (1.44) than those fed soy-lecithin (1.91 and 1.25 respectively). The growth pattern, feed and protein efficiency measures were not only better with lyso-lecithin compared to soy-lecithin and also on par with the control group. However, there was no significant difference in survival (82.22 to 84.44%) between the dietary treatments. The fatty acid composition of diets to a certain extent was reflected in the carcass composition of shrimp. The most essential fatty acids &lt;em&gt;viz.,&lt;/em&gt; eicosapentaenoic and docosahexaenoic acids were significantly (p&amp;lt;0.05) higher in shrimp fed lyso-lecithin supplemented diet. Present results suggest that lyso-lecithin enhances growth performance of &lt;em&gt;P. monodon&lt;/em&gt; and carcass fatty acid composition compared to soy-lecithin due to its high emulsification properties with partial replacement of marine sources.&lt;/p&gt;

Dietary inclusion of mussel meal enhances performance and improves feed and protein utilization in common sole (Solea solea, Linnaeus, 1758) juveniles

The present study was carried out to test different mussel meal (MM) dietary levels in combination with fishmeal (FM) on the growth performance, fatty acid composition and liver histology of common sole, Solea solea juveniles to highlight the growth potential of this species. Four isoproteic (53%) and isolipidic (11%) pelletized diets were formulated to contain graded levels of mussel meal, MM0 (0%), MM25 (25%), MM50 (50%) and MM75 (75%), up to 75%. Sole juveniles (initial individual mean body weight 13.1 ± 2.3 g, n = 840) were fed to satiation for 91 days. Seventy fish per tank (500-L, 0.64 m2 bottom surface) were reared in 12 tanks (3 tanks per treatment) at 20 ± 1°C. Diets containing MM (MM25, MM50 and MM75) gave a significantly higher specific growth rate (SGR, 1.27 ± 0.01, 1.38 ± 0.06 and 1.40 ± 0.05, respectively), higher feed intake and lower feed conversion rate (FCR, 1.09 ± 0.01, 1.00 ± 0.04 and 0.98 ± 0.02, respectively) when compared to the FM-based diet (MM0, SGR, 0.98 ± 0.11, FCR, 1.52 ± 0.13). Carcass proximate composition was not influenced by dietary treatments, with the exception of the significantly lower lipid content in the MM75 group. Protein efficiency ratio (PER) and gross protein efficiency (GPE) were significantly improved by the mussel meal inclusion (PER, 1.29 ± 0.12, 1.76 ± 0.01, 1.89 ± 0.06, 1.95 ± 0.08; GPE, 25.29 ± 1.85, 33.38 ± 0.89, 35.96 ± 1.36, 36.59 ± 1.05 in MM0, MM25, MM50 and MM75, respectively). A significant decrease in the viscerosomatic index was observed in fish fed with MM50 and MM75 in comparison to MM0. The hepatosomatic index of fish fed with MM0 and MM25 was higher than that of fish fed with MM75, although the histological examination of liver parenchyma in all experimental groups showed a uniformly abundant accumulation of lipid droplets. Carcass fatty acid composition was significantly affected by dietary treatments, reflecting the dietary fatty acid profile. According to these results, the inclusion of MM in experimental FM-based diets improved the performance and feed utilization of common sole juveniles. The inclusion of MM in the present trial allowed a higher SGR than that registered in previous growth trials on common sole. This study could provide useful information to detect effective ingredients for practical diets in Solea solea. It also seems advisable to consider an inclusion of at least 25% MM in the experimental reference diet to be used for further application towards the development of specific diets for this species.

Identification of exonic nucleotide variants of the thyroid hormone responsive protein gene associated with carcass traits and Fatty Acid composition in korean cattle.

The thyroid hormone responsive protein (THRSP) gene is a functional gene that can be used to indicate the fatty acid compositions. This study investigates the relationships of exonic single nucleotide polymorphisms (SNPs) in the THRSP gene and fatty acid composition of muscle fat and marbling score in the 612 Korean cattle. The relationships between fatty acid composition and eight SNPs in the THRSP gene (g.78 G>A, g.173 C>T, g.184 C>T, g.190 C>A, g.194 C>T, g.277 C>G, g.283 T>G and g.290 T>G) were investigated, and according to the results, two SNPs (g.78 G>A and g.184 C>T) in exon 1 were associated with fatty acid composition. The GG and CC genotypes of g.78 G>A and g.184 C>T had higher unsaturated fatty acid (UFA) and monounsaturated fatty acid (MUFA) content (p<0.05). In addition, the ht1*ht1 group (Val/Ala haplotype) in a linkage disequilibrium increased MUFAs and marbling scores for carcass traits (p<0.05). As a result, g.78 G>A and g.184 C>T had significantly relationships with UFAs and MUFAs. Two SNPs in the THRSP gene affected fatty acid composition, suggesting that GG and CC genotypes and the ht1*ht1 group (Val/Ala haplotype) can be markers to genetically improve the quality and flavor of beef.

Effects of dietary byproduct feeding withdrawal prior to market on finishing pig growth performance, carcass characteristics, carcass fat quality, intestinal weights, and economics (2013)

A total of 288 pigs (PIC 327 × 1050; initially 84.7 lb) were used in an 88-d study to determine the timing of high-fiber ingredient removal from the diet prior to marketing to optimize growth performance, carcass characteristics (primarily yield), carcass fatty acid composition, and economics. Two diet types, a corn-soybean meal control diet with low NDF (9.3%) and a high-fiber, high-NDF (19%) diet that contained 30% dried distillers grains with solubles (DDGS) and 19% wheat middlings (midds) were used throughout the study. Pens of pigs were randomly allotted to 1 of 6 dietary feeding strategies with 8 pigs per pen (4 barrows and 4 gilts) and 6 replications per treatment. The 6 feeding strategies consisted of the corn-soy control diet or high-fiber diet fed for the duration of the study, or the high-fiber diet fed until 20, 15, 10, or 5 d prior to slaughter after which the pigs were switched to the corn-soybean meal control diet.

Effect of Feeding Various Amounts of Pacific Sandeel Flakes on Growth, Survival and Carcass Fatty Acid Composition of Common Octopus Octopus vulgaris Paralarvae

Effect of Feeding Various Amounts of Pacific Sandeel Flakes on Growth, Survival and Carcass Fatty Acid Composition of Common Octopus Octopus vulgaris Paralarvae

Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer

Omega-3(omega-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of omega-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene.

Effect of source of dietary fat on pig performance, carcass characteristics and carcass fat content, distribution and fatty acid composition

Seventy gilts were used to compare the effect of including 10% tallow (T), high-oleic sunflower oil (HOSF), sunflower oil (SFO), linseed oil (LO), a fat blend (FB), or an oil blend (OB) in finishing diets vs. feeding a semi-synthetic diet with no added fat (NF) on pig performance, carcass traits and carcass fatty acid (FA) composition. Carcasses from SFO-fed gilts had greater fat and lower lean compositions than carcasses from T-fed gilts. Gilts fed NF had greater loin fat than FB-fed gilts, and greater flare fat, loin intermuscular fat and fat:lean than T-fed gilts. Bellies from NF-fed gilts had lower lean and higher intermuscular fat and fat:lean than other diets except HOSF. Fat source had minor effects on animal performance, carcass characteristics and carcass fat content and distribution, whereas feeding NF resulted in carcasses and major cuts with higher fat content. Diets rich in polyunsaturated FA (PUFA) did not reduce fat deposition in separable fat depots with respect to monounsaturated FA (MUFA) and saturated FA (SFA). Carcasses from gilts fed NF had a high degree of saturation (40.6% SFA) followed by carcasses of T- and FB-fed gilts. Feeding HOSF, SFO and LO enriched diets elevated the percentages of MUFA (56.7%), n-6 (30.0%) and n-3 (16.6%) PUFA, respectively, whereas carcasses from gilts fed OB had greater percentages of n-3 FA (14.8% n-3, 0.9% EPA, 1.0% DPA, 3.1% DHA) than gilts fed FB (6.72% n-3, 0.1% EPA, 0.4% DPA, 0.1% DHA).

Effect Of Fat Source And Vitamin E Supplimentation On Fat Composition And Keeping Quality Of Broiler Meat

The effect of different sources of fat on carcass characteristics, fatty acid composition of broilers and the interactive effect of fat source and vitamin E on keeping quality of broiler thigh meat were evaluated. The four dietary treatments were the control (No fat inclusion); palm oil; groundnut oil and palm kernel oil. The diets were isocaloric and isonitrogenous with the oils providing equal kilocalories of 332 ME /kg and 415 ME / kg in both the starter and finisher diets respectively. Seventy two (72) broiler chicks of six (6) birds per replicate (18 birds per treatment) were utilized for this experiment. At the 8th week of experiment, three birds per treatment were slaughtered for carcass evaluation and fatty acid composition of the total lipids from the adipose tissue was determined. The remaining birds on each experimental diet was then redistributed and divided in to two sub-groups; one group was given 100mg /kg vitamin E in their diet while the other group received no Vitamin E. The birds were further fed a broiler finisher diet for two weeks, after which two birds from each sub group were used for the determination of the keeping quality of broiler thigh meat frozen at 120c for 0, 14 and 21days as measured by the thiobarbituric acid (TBA) number.The dressing percentage, abdominal, thigh and skin fat contents were not significantly (P>0.05) affected by fat sources. However, birds fed diets containing supplemental fat consistently had higher dressing percentage, abdominal, thigh and skin fat contents. In all, birds on diets containing groundnut oil had the highest values.The fatty acid compositions of the total lipid from the adipose tissues were significantly different from each other. However the characteristics of each dietary fat were incorporated into the adipose tissue. Oleic acid (18:1) and palmitic (16:0) were high in birds fed the control diet, palm oil and groundnut oil containing diets while lauric acid (12:0) and palmitic (16:0) acid were high in the diet containing palm kernel oil. Inclusion of Vitamin E Supplementation in the diet had a significant (P 0.05) interaction between fat source, vitamin E supplementation and the length of freeze storage on the keeping quality of broiler meat.

Effect of Improved Housing Design and Sexing on Carcass Traits and Fatty Acid Composition of Meat in Broiler Chicken

Simsek, U.G., Ciftci, M., Cerci, I.H., Gurses, M. and Guvenc, M. 2009. Effect of improved housing design and sexing on carcass traits and fatty acid composition of meat in broiler chicken. J. Appl. Anim. Res., 36: 219–222. To study the effects of improved housing design (sand bedding and provision of perches) and sexing on carcass traits and fatty acid composition, 480 Ross-308 chicks were assigned to two groups including equal number of male and female. At d 42, 8 representative males and 8 females were slaughtered from each group. Their carcasses were evaluated. Fatty acid composition of whole carcass (4 males, 4 females) and meat from breast and left thigh from the remaining 4 males and 4 females were evaluated. Carcass traits did not differ between groups. Total PUFA, ω-3 and ω-6 ratios increased and total SFA and MUFA ratios decreased (P<0.001), in the improved housing group. Total SFA was found to be higher in female than male broiler (P<0.05). It was concluded that improved housing with perches and sand bedding improved chicken meat quality and increased total polyunsaturated fatty acid ratio of the meat. Sexing significantly affected fatty acids composition of meat.

Growth and carcass fatty acid composition of beef steers fed soybean oil for increasing duration before slaughter

Duration of soybean oil (SBO) supplementation needed to enhance carcass conjugated linoleic acid (CLA) and trans-vaccenic (TVA) content was examined using 96 beef steers (293.6 ± 3.9 kg) fed a 78% corn-based diet supplemented with SBO for 0, 77, 137, or 189 days before slaughter. Duration of SBO supplementation had no effect ( P ⩾ 0.15) on animal performance or carcass traits, nor ( P ⩾ 0.15), total, total saturated, or total polyunsaturated fatty acids of Longissimus dorsi (LD). Concentrations of CLA in LD were not affected ( P ⩾ 0.18) by SBO supplementation. Concentrations of monounsaturated fatty acids (MUFA) decreased linearly ( P = 0.03) in LD, whereas TVA increased ( P = 0.04) in adipose tissue and tended ( P = 0.07) to increase in LD with increasing duration of SBO supplementation. Supplementing SBO to a concentrate-based diet may enhance TVA without impacting CLA, while reducing the MUFA content of lean beef.

Effects of supplemental soybean oil and vitamin E on carcass quality and fatty acid profiles of meat in Huzhou lamb

This study was conducted to determine the effects of supplemental soybean oil (SBO) and vitamin E (VE) on carcass characteristics, serum metabolites, and fatty acid (FA) composition of longissimus muscle in Huzhou lambs. Totally, 24 male lambs were allocated to three dietary treatments: basal, 3% SBO, and 3% SBO plus 500 mg/kg VE supplemented diet (SOE). No differences were observed in carcass qualities among the three treatments. Contents of serum cholesterol, urea nitrogen, and non-esterified fatty acid increased, while glucose decreased significantly in SBO and SOE groups compared to control (P<0.05). The SBO and SOE diets increased the polyunsaturated fatty acids and cis 9 trans11 conjugated linoleic acid (CLA; P<0.05), but the ratio of n-6 to n-3 was not effected. It is concluded that supplementation of SBO could increase CLAs, but no further increase may be expected by additional supplementation of VE.

Dietary utility of enzyme-treated fish meal for juvenile Pacific bluefin tuna Thunnus orientalis

In order to develop an artificial diet, the dietary utility of enzyme-treated fish meal was investigated for juvenile Pacific bluefin tuna Thunnus orientalis (PBT). Diets containing each 63% of Chilean fish meal (FM), enzyme-treated chilean fish meal (EC) and enzyme-treated Peruvian fish meal (EP), with 10% bonito oil and raw sand lance Ammodytes personatus (SL) were fed to juvenile tuna six times per day for one week. In a different trial, diets EC and SL were fed to tuna six times per day for 2 weeks. Only diet EC sustained similar growth or caused lower survival and higher feed efficiency, hepato- and enterosomatic indices and final carcass lipid content as compared to those of SL. Diets FM and EP led to lower specific growth rate (SGR) but similar feed efficiency, survival and hepatosomatic index, yet higher enterosomatic index. Moreover, PBT fed diet EC for 2 weeks led to similar growth performance but higher final carcass and hepatic lipid contents, and plasma cholesterol and phospholipid levels than those fed SL. Carcass fatty acid composition of diet EC group had lower 20∶5 n-3 and 22∶6 n-3 levels than the SL group. These results revealed that EC, as a suitable dietary protein source, could sustain growth of PBT, while dietary bonito oil led to higher carcass lipid but lower accumulation of n-3 highly unsaturated fatty acids.