Owing to the multi-dimensional complexity of human motions, traditional uniaxial strain sensors lack the accuracy in monitoring dynamic body motions working in different directions, thus multidirectional strain sensors with excellent electromechanical performance are urgently in need. Towards this goal, in this work, a stretchable biaxial strain sensor based on double elastic fabric (DEF) was developed by incorporating carboxylic multi-walled carbon nanotubes(c-MWCNTs) and polypyrrole (PPy) into fabric through simple, scalable soaking and adsorption-oxidizing methods. The fabricated DEF/c-MWCNTs/PPy strain sensor exhibited outstanding anisotropic strain sensing performance, including relatively high sensitivity with the maximum gauge factor (GF) of 5.2, good stretchability of over 80%, fast response time < 100 ms, favorable electromechanical stability, and durability for over 800 stretching–releasing cycles. Moreover, applications of DEF/c-MWCNTs/PPy strain sensor for wearable devices were also reported, which were used for detecting human subtle motions and dynamic large-scale motions. The unconventional applications of DEF/c-MWCNTs/PPy strain sensor were also demonstrated by monitoring complex multi-degrees-of-freedom synovial joint motions of human body, such as neck and shoulder movements, suggesting that such materials showed a great potential to be applied in wearable electronics and personal healthcare monitoring.