Carboxen/polydimethylsiloxane Research Articles

Volatile profile of Atlantic shellfish species by HS-SPME GC/MS

A procedure based on headspace solid-phase microextraction (HS-SPME) and GC–MS analysis was developed to investigate the volatile organic compounds (VOCs) of Atlantic shellfish species. The experimental conditions (fiber coating, sample volume, time and temperature of incubation) were optimized for the extraction of VOCs from shellfish. The incubation of a Carboxen/Polydimethylsiloxane (CAR-PDMS) fiber with 6mL of saline aqueous extract heated at 80°C for 30min gave the most effective and accurate extraction of the analytes. Under these experimental conditions, thermal degradations of volatiles that could be occurred during the extraction process were not observed. The method was validated in terms of linearity, repeatability, inter-day precision, recovery and sensitivity. Then, the optimized method was applied to study the volatile profile of mussels (Mytilus galloprovincialis), pullet carpet shells (Venerupis pullastra), oysters (Ostrea edulis), pod razor shells (Ensis ensis), common cockle (Cerastoderma edule) and goose barnacle (Pollicipes cornucopia). The results showed significant differences in the volatile profile, resulting some of the compound characteristic for the shellfish species. The fatty acid profile has been also investigated and the occurrence of non methylen interrupted fatty acids (NMI) in these species was related with specific volatiles. The present investigation provides the first VOC description of Atlantic E. ensis, V. pullastra, C. edule and P. cornucopia and highlights the important contribution of SPME methodology to identify volatile markers of the natural flavor of seafood, which are useful for the evaluation of origin and represent a starting point to find useful volatiles as freshness markers before commercialization or changes due to processing.

Time-Weighted Average Sampling of Airborne Propylene Glycol Ethers by a Solid-Phase Microextraction Device

A solid-phase microextraction (SPME) device was used as a diffusive sampler for airborne propylene glycol ethers (PGEs), including propylene glycol monomethyl ether (PGME), propylene glycol monomethyl ether acetate (PGMEA), and dipropylene glycol monomethyl ether (DPGME). Carboxen-polydimethylsiloxane (CAR/PDMS) SPME fiber was selected for this study. A polytetrafluoroethylene (PTFE) tubing was used as the holder, and the SPME fiber assembly was inserted into the tubing as a diffusive sampler. The diffusion path length and area of the sampler were 0.3 cm and 0.00086 cm2, respectively. The theoretical sampling constants at 30°C and 1 atm for PGME, PGMEA, and DPGME were 1.50 × 10−2, 1.23 × 10−2 and 1.14 × 10−2 cm3 min−1, respectively. For evaluations, known concentrations of PGEs around the threshold limit values/time-weighted average with specific relative humidities (10% and 80%) were generated both by the air bag method and the dynamic generation system, while 15, 30, 60, 120, and 240 min were selected as the time periods for vapor exposures. Comparisons of the SPME diffusive sampling method to Occupational Safety and Health Administration (OSHA) organic Method 99 were performed side-by-side in an exposure chamber at 30°C for PGME. A gas chromatography/flame ionization detector (GC/FID) was used for sample analysis. The experimental sampling constants of the sampler at 30°C were (6.93 ± 0.12) × 10−1, (4.72 ± 0.03) × 10−1, and (3.29 ± 0.20) × 10−1 cm3 min−1 for PGME, PGMEA, and DPGME, respectively. The adsorption of chemicals on the stainless steel needle of the SPME fiber was suspected to be one of the reasons why significant differences between theoretical and experimental sampling rates were observed. Correlations between the results for PGME from both SPME device and OSHA organic Method 99 were linear (r = 0.9984) and consistent (slope = 0.97 ± 0.03). Face velocity (0–0.18 m/s) also proved to have no effects on the sampler. However, the effects of temperature and humidity have been observed. Therefore, adjustments of experimental sampling constants at different environmental conditions will be necessary.

Headspace solid-phase microextraction for the determination of volatile sulfur compounds in odorous hyper-eutrophic freshwater lakes using gas chromatography with flame photometric detection

A simple and efficient method based on headspace solid-phase microextraction (HS-SPME) followed by gas chromatography coupled to flame photometric detection was developed for the simultaneous determination of five volatile sulfur compounds (VSCs; hydrogen sulfide (H2S), methanethiol (MeSH), dimethyl sulfide (DMS), dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS)) in hyper-eutrophic freshwater lakes suffering from black water agglomerate. Carboxen/polydimethylsiloxane (CAR/PDMS) fiber was selected for the extraction of VSCs, and various parameters, such as the stirring rate, sample volume, ionic strength, duration and temperature of extraction and the duration and temperature of desorption, were optimized for extraction efficiency. The linearity spanned approximately three orders of magnitude for all of the studied compounds. The method detection limits (MDLs) and method quantification limits (MQLs) ranged from 1.6 to 93.5ngL−1 and 10 to 500ngL−1, respectively, depending on the analyzed compound. The reproducibility of the method was between 3.7 and 11.9%, and the recovery was approximately 100%. The developed analytical method was successfully applied to the determination of VSCs in odorous freshwater lakes, and the concentrations of the five target compounds were detected in the 0.33 to 166.60μgL−1 range.

Volatile compounds of the Hallabong (Citrus kiyomi × Citrus ponkan) blossom

The objective of this study was to identify and compare the volatile compounds of liquid essential oils and volatiles of headspace-solid phase microextraction (HSSPME) of the hallabong blossom. SPME is a sampling technique based on sorption of analytes into a polymeric material that coats a silica fiber. Two different fiber coatings tested to evaluate the extraction efficiencies of volatiles. The hallabong blossom exhibited 5 major volatile compounds: linalool (28.89–43.24%), limonene (0.39–23.06%), β-myrcene (0.38–16.67%), sabinene (1.37–11.42%), and 4-terpineol (1.26–6.12%). The amounts of identified compounds in the hallabong blossom were more abundant in carboxen/polydimethylsiloxane (CAR/PDMS) fiber than in PDMS fiber.

Analysis of haloacetic acids in water and air (aerosols) from indoor swimming pools using HS-SPME/GC/ECD

A solid phase microextraction method was used for the analysis of nine haloacetic acids (HAAs) in water and air (aerosols) from indoor swimming pools (ISPs). The analysis is characterized by derivatization of HAAs to their methyl-esters with dimethyl sulphate, headspace solid phase microextraction (HS-SPME) with a Carboxen–polydimethylsiloxane (CAR-PDMS) fiber and gas chromatography - electron capture detector (GC/ECD). High correlation coefficients were obtained for esters mixture calibration lines and detection limits were found to be at the low ppb level. Repeatability was assessed and coefficients of variation varied from 10 to 20%. Reproducibility was also evaluated and coefficients of variation from 15 to 25% were obtained. Analytical results from four Portuguese ISPs showed that the mean concentration of total HAAs (THAAs) in water ranged from 10 ± 2 to 183 ± 28 μg/L in which 55 ± 20% corresponded to trichloroacetic and dichloroacetic acids (TCAA and DCAA). THAAs highest concentrations were directly related to higher ISPs’ water organic matter content. In the lack of European specific regulation for water from ISPs and taking into consideration that ingestion is a form of exposure, THAAs concentration values were compared with drinking water maximum contamination level (MCL) of 60 μg/L proposed by the US EPA for the sum of five HAAs. In 35% of water sampling campaigns the sum of MBAA (monobromoacetic acid), MCAA (monochloroacetic acid), DCAA and TCAA exceeded that MCL value. The concentrations obtained for THAAs in the ISPs’ atmosphere ranged from 5 ± 1 to 64 ± 10 μg/m3 (T = 28°C at 5 cm above the water surface) and were proportional to the aerosols’ quantity, which was deeply related to indoor air ventilation system.

Simple determination of formaldehyde in fermented foods by HS‐SPME‐GC/MS

SummaryWe describe an automatic method to detect formaldehyde (FA) in some fermented foods. This method is based on derivatisation with 2,2,2‐trifluoroethylhydrazine (TFEH) and consecutive headspace solid‐phase micro‐extraction and gas chromatography‐mass spectrometry. FA in food reacted for 30 min at 40 °C with 2,2,2‐TFEH in a headspace vial, and the formed FA‐TFEH derivative was simultaneously vaporised and adsorbed on 85‐μm carboxen–polydimethylsiloxane. Under the established condition, the limit of detection was 0.1 μg kg−1 by using 1.0‐g solid food and 1.0‐mL liquid food, and the relative standard deviation was less than 10% at FA concentrations between 0.050 and 0.500 mg kg−1. The concentrations of FA in several traditional Korean foods including gimchi, watery radish gimchi, soybean paste, red pepper paste, soy sauce and bean‐paste soup were measured. All food samples had detectable levels of FA (0.104–13.05 mg kg−1).

Investigation of urinary volatile organic metabolites as potential cancer biomarkers by solid-phase microextraction in combination with gas chromatography-mass spectrometry

Background:Non-invasive diagnostic strategies aimed at identifying biomarkers of cancer are of great interest for early cancer detection. Urine is potentially a rich source of volatile organic metabolites (VOMs) that can be used as potential cancer biomarkers. Our aim was to develop a generally reliable, rapid, sensitive, and robust analytical method for screening large numbers of urine samples, resulting in a broad spectrum of native VOMs, as a tool to evaluate the potential of these metabolites in the early diagnosis of cancer.Methods:To investigate urinary volatile metabolites as potential cancer biomarkers, urine samples from 33 cancer patients (oncological group: 14 leukaemia, 12 colorectal and 7 lymphoma) and 21 healthy (control group, cancer-free) individuals were qualitatively and quantitatively analysed. Dynamic solid-phase microextraction in headspace mode (dHS-SPME) using a carboxen-polydimethylsiloxane (CAR/PDMS) sorbent in combination with GC-qMS-based metabolomics was applied to isolate and identify the volatile metabolites. This method provides a potential non-invasive method for early cancer diagnosis as a first approach. To fulfil this objective, three important dHS-SPME experimental parameters that influence extraction efficiency (fibre coating, extraction time and temperature of sampling) were optimised using a univariate optimisation design. The highest extraction efficiency was obtained when sampling was performed at 50°C for 60 min using samples with high ionic strengths (17% sodium chloride, w v−1) and under agitation.Results:A total of 82 volatile metabolites belonging to distinct chemical classes were identified in the control and oncological groups. Benzene derivatives, terpenoids and phenols were the most common classes for the oncological group, whereas ketones and sulphur compounds were the main classes that were isolated from the urine headspace of healthy subjects. The results demonstrate that compound concentrations were dramatically different between cancer patients and healthy volunteers. The positive rates of 16 patients among the 82 identified were found to be statistically different (P<0.05). A significant increase in the peak area of 2-methyl-3-phenyl-2-propenal, p-cymene, anisole, 4-methyl-phenol and 1,2-dihydro-1,1,6-trimethyl-naphthalene in cancer patients was observed. On average, statistically significant lower abundances of dimethyl disulphide were found in cancer patients.Conclusions:Gas chromatographic peak areas were submitted to multivariate analysis (principal component analysis and supervised linear discriminant analysis) to visualise clusters within cases and to detect the volatile metabolites that are able to differentiate cancer patients from healthy individuals. Very good discrimination within cancer groups and between cancer and control groups was achieved.

Determination of Volatile Nitrosamines in Latex Products by HS-SPME–GC–MS

Nitrosamines which have been detected in various latex products are carcinogens. The method for determination of volatile nitrosamines in latex products was developed using a combination of headspace solid phase micro-extraction (HS-SPME) and gas chromatography–mass spectrometry (GC–MS). A carboxen/polydimethylsiloxane (CAR/PDMS) fiber was used for HS-SPME involving the following variables: (1) agitation conditions, (2) extraction temperature (3) extraction time, and (4) salt concentration. The instrument performances of three detection systems including GC combined thermal energy analyzer, nitrogen chemiluminescence detector and MS were evaluated. The agitation conditions including magnetic stirring and ultrasonication were investigated by the comparison of extraction efficiency of HS-SPME for nitrosamines. Obtained optimal detection conditions of nitrosamines were HS-SPME at 45 °C for 60 min assisted with magnetic stirring and saturated NaCl followed by GC–MS. To evaluate this method performance, the commercial products including eleven latex products (gloves, balloons and condoms) and four liquid silicone nipples were analyzed with the method. The results revealed that the method is suitable for simple and effective determination of nitrosamines in latex products. The advantage of this HS-SPME–GC–MS method is simple treatment, fast analysis, adequate sensitivity and without organic solvent.

Effects of an Industrial Milling Process on Change of Headspace Volatiles in Yihchuan Aromatic Rice

ABSTRACTHeadspace volatiles of Yihchuan aromatic rice, a japonica rice cultivar, were extracted by a solid‐phase microextraction (SPME) method and analyzed by GC and GC‐MS. Effects of fiber types and an industrial milling process on the change of headspace volatiles were studied. Of the fiber types, divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS), polydimethylsiloxane/divinylbenzene (PDMS/DVB), and carboxen/polydimethylsiloxane (CAR/PDMS) were suggested for use in the analysis headspace volatiles in terms of absorption or adsorption ability. Regarding the milling effect, the amount of total headspace volatiles from brown rice to white rice decreased ≈30%, and that of aldehydes and alcohols, the two largest groups of volatiles, decreased ≈20 and 15%, respectively. Comparatively, rice bran contained higher amounts of total headspace volatiles than milled rice grains.

Analysis of volatile compounds of Antrodia camphorata in submerged culture using headspace solid-phase microextraction

In this work a headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) and GC–olfactometry (GC–O) was developed to evaluate the profile of the volatile compounds that contribute to the aroma of Antrodia camphorata in submerged culture. For this purpose, the HS-SPME sampling method for the volatile compounds of A. camphorata in submerged culture was optimised by a D-optimal design. A HS extraction of the culture broth of A. camphorata followed by incubation on a carboxen/polydimethylsiloxane (CAR/PDMS) fibre during 31.8min at 54.6°C gave the most effective and accurate extraction of the volatile compounds. By the optimised method, a total of 49 volatile compounds were identified in culture broth of A. camphorata, while a total of 55 volatile compounds were identified in the mycelia. A series of C8 aliphatic compounds (mushroom-like odour), several lactones (fruity odour) and l-linalool (citrus-like odour) were the most potent key odourant in both the mycelia and culture broth. This combined technique is fast, simple, sensitive, inexpensive and useful to monitor volatile compounds associated to A. camphorata.

Determination of volatile organic compounds in recycled polyethylene terephthalate and high-density polyethylene by headspace solid phase microextraction gas chromatography mass spectrometry to evaluate the efficiency of recycling processes

A method for the determination of volatile organic compounds (VOCs) in recycled polyethylene terephthalate and high-density polyethylene using headspace sampling by solid-phase microextraction and gas chromatography coupled to mass spectrometry detection is presented. This method was used to evaluate the efficiency of cleaning processes for VOC removal from recycled PET. In addition, the method was also employed to evaluate the level of VOC contamination in multilayer packaging material containing recycled HDPE material. The optimisation of the extraction procedure for volatile compounds was performed and the best extraction conditions were found using a 75 μm carboxen-polydimethylsiloxane (CAR-PDMS) fibre for 20 min at 60 °C. The validation parameters for the established method were linear range, linearity, sensitivity, precision (repeatability), accuracy (recovery) and detection and quantification limits. The results indicated that the method could easily be used in quality control for the production of recycled PET and HDPE.

Characterisation of volatile components of Pinotage wines using comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC × GC–TOFMS)

As part of the ongoing research into the chemical composition of the uniquely South African wine cultivar Pinotage, the volatile composition of nine young wines of this cultivar was investigated using comprehensive two-dimensional gas chromatography (GC × GC) in combination with time-of-flight mass spectrometry (TOFMS). Headspace solid phase micro-extraction (HS-SPME) using a carboxen/polydimethylsiloxane (CAR/PDMS) fibre was used to extract the volatile compounds from the wine matrix. Extracts were analysed using an in-house developed GC × GC system equipped with a single jet, liquid nitrogen-based cryogenic modulator. In the current study, 206 compounds previously reported in wine and related matrices have been detected in nine Pinotage wines. Positive identification for 48 compounds was performed using authentic standards, while tentative identification of 158 compounds was based on deconvoluted mass spectra and comparison of linear retention indices (LRI) with literature values. Identified compounds included esters, alcohols, aldehydes, ketones, acids, acetals, furans and lactones, sulphur compounds, nitrogen compounds, terpenes, hydrocarbons, volatile phenols and pyrans. Volatile compounds potentially capable of influencing wine aroma are highlighted. Many of the compounds were common to all 9 wines, although volatile components unique to specific samples were also observed. The results represent the most detailed characterisation of volatile constituents of this cultivar reported to date.

Volatile compounds of the cultivated dumebuchu (Allium senescens L. var. senescens)

This study was conducted to examine the volatile compounds of cultivated dumebuchu (Allium senescens L. var. senescens). Dumebuchu is listed as a rare plant in Korea by Korea National Arboretum and has peculiar and long-lasting odor which resembles the flavor of garlic and onion. The volatile compounds of the cultivated dumebuchu were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GCMS). The field-cultivated dumebuchu had a larger amount of terpene compounds than greenhouse-cultivated one. The total of 9 sulfur containing compounds was identified in the cultivated dumebuchu. The most abundant compound was dipropyl disulfide and the second most abundant compound was propyl allyl disulfide. Additionally, carboxen/polydimethylsiloxane (CAR/PDMS) fiber was found to be more efficient than PDMS fiber for extracting sulfur containing compounds from the cultivated dumebuchu.

Headspace Volatiles of Three Turkish Plants

Headspace volatiles of fresh flowers of Stenbergia candida Mathew et Baytop (Amaryllidaceae) and Muscari muscarimi Medikus (Liliaceae), and crushed bulbs of Nectaroscordum siculum (Ucria) Lindl. (Liliaceae) were analyzed by gas chromatography/mass spectrometry. The volatiles were trapped by SPME in a dynamic headspace set up. Stenbergia flowers red—Polydimethylsiloxane (PDMS), blue—Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) and black—Carboxen/Polydimethylsiloxane (CAR/PDMS) flbers were used. (E)-β-ocimene (50–81%) and nerol (2–19%) were found as main components. Volatiles of Muscari muscarimi were trapped by blue SPME flber over a period of three days. Main components were identified as (E)-β-ocimene (t-36%), methyl salicylate (1–21%), E-methyl isoeugenol (4–22%) and benzyl benzoate (7–56%). Volatiles of crushed bulbs of Nectaroscordum siculum were trapped on an HS-SPME (Red flber). Only two components were found to be dibutyl disulfide (88.5%) and 2-hexyl-5-methyl-(2H)-furan-3-one (11.5%).

(E)-2-Nonenal determination in brazilian beers using headspace solid-phase microextraction and gas chromatographic coupled mass spectrometry (HS-SPME-GC-MS)

(E)-2-nonenal is considered an important off-flavor of beer, related to the flavor of beer staling. In this study, a new method for determination of (E)-2-nonenal in beer using headspace solid-phase microextraction and gas chromatographic coupled mass spectrometry (HS-SPME-GC-MS) was developed and applied in Brazilian beer samples. The extractions were carried out in CAR-PDMS (carboxen-polydimethylsiloxane) fiber and the best results were found with 15 minutes of equilibrium and 90 minutes of extraction at 50 °C. The method was linear in the range from 0.02 to 4.0 μg.L-1 with correlation coefficient of 0.9994. The limits of detection and quantification were 0.01 and 0.02 μg.L-1, respectively. 96.5% of recovery and 4% precision (RSD) were obtained in the fortification of beer samples with 2.0 μg.L-1 of (E)-2-nonenal. The developed method proved to be simple, efficient and highly sensitive to the determination of this analyte being easily applied in the quality control of the brewery. (E)-2-nonenal was found in all beer samples analyzed with levels between 0.17 and 0.42 μg.L-1.

Volatile Compounds in Honey Produced in the Central Valley of Ñuble Province, Chile

Headspace solid-phase microextraction (SPME) with an 85 µm Carboxen polydimethylsiloxane (CAR/PDMS) fiber was used to extract volatile compounds, and a gas chromatograph equipped with a mass spectometry detector (GCMS) was used to identify the volatile compounds in honeys. Thirty-four different volatile compounds from the headspace of honey produced in the central valley of Nuble Province, Chile, were extracted with fiber coating CAR/PDMS. The identified compounds were: 10 alcohols, 9 acids, 6 ketones, 3 aldehydes, 2 furans, 2 terpenes and 2 lactones. Only four of the volatile compounds had never been reported before as honey compounds; these being: 1,3-propanodiol, 2-methyl butanoic acid, 3,4-dimethyl-3-hexen-2-one, and 6-methyl-5-octen-2-one. These four compounds were found in three of the 10 analyzed samples. The compounds found in the highest percentage of area were ethanol, acetic acid, 1-hydroxy-2-propane, 3-hydroxy-2-butane, and furfural. However, the analyzed samples did not present a distinctive profile.

A new polyethylene glycol fiber prepared by coating porous zinc electrodeposited onto silver for solid-phase microextraction of styrene

A new polyethylene glycol fiber was developed for solid-phase microextraction (SPME) of styrene by electrodepositing porous Zn film on Ag wire substrate followed by coating with polyethylene glycol sol–gel (Ag/Zn/PEG sol–gel fiber). The scanning electron micrographs of fibers surface revealed a highly porous structure. The extraction property of the developed fiber-to-styrene residue from polystyrene packaged food was investigated by headspace solid-phase microextraction (HS-SPME) and analyzed with a gas chromatograph coupled with flame ionization detection (GC-FID). The new Ag/Zn/PEG sol–gel fiber is simple to prepare, low cost, robust, has high thermal stability and long lifetime, up to 359 extractions. Repeatability of one fiber ( n = 6) was in the range of 4.7–7.5% and fiber-to-fiber reproducibility ( n = 4) for five concentration values were in the range 3.4–10%. This Ag/Zn/PEG sol–gel fiber was compared to two commercial SPME fibers, 75 μm carboxen/polydimethylsiloxane (CAR/PDMS) and 100 μm polydimethylsiloxane (PDMS). Under their optimum conditions, Ag/Zn/PEG sol–gel fiber showed the highest sensitivity and the lowest detection limit at 0.28 ± 0.01 ng mL −1.

Analytical performance of three commonly used extraction methods for the gas chromatography–mass spectrometry analysis of wine volatile compounds

The analytical performance of three extraction procedures based on cold liquid–liquid extraction using dicloromethane (LLE), solid phase extraction (SPE) using a styrene–divinylbenzene copolymer and headspace solid phase microextraction (SPME) using a carboxen–polydimethylsiloxane coated fibre has been evaluated based on the analysis of 30 representative wine volatile compounds. From the comparison of the three procedures, LLE and SPE showed very good linearity covering a wide range of concentrations of wine volatile compounds, low detection limits, high recovery for most of the volatile compounds under study and higher sensitivity compared to the headspace-SPME procedure. The latter showed in general, poor recovery for polar volatile compounds. Despite some drawbacks associated with the LLE and SPE procedures such as the more tedious sampling treatment and the use of organic solvents, the analytical performance of both procedures showed that they are more adequate for the analysis of wine volatiles.

Profiling volatile compounds from Mucuna beans by solid phase microextraction and gas chromatography-high resolution time of flight mass spectrometry

Abstract Four solid phase microextraction (SPME) fibres, polydimethylsiloxane (PDMS), polydimethylsiloxane/divinylbenzene (PDMS/DVB), polyacrylate (PA), and carboxen/polydimethylsiloxane (CAR/PDMS), were evaluated for profiling of volatile compounds during cooking of Mucuna pruriens beans. A gas chromatograph coupled to a high resolution time of flight mass spectrometer system was employed for separation, detection and identification of the volatile compounds. For the first time we report a total of 26 compounds, mostly alkyl benzenes and polycyclic compounds, identified in black, white, black–white, and yellow green Mucuna beans during head space sampling employing a CAR/PDMS fibre with subsequent detection with high resolution mass spectrometry. The number of volatile compounds sampled, most notably alkylbenzenes, decreased with each hour of boiling and discarding of water extracts. As the beans approached being fully cooked, benzoic acid 2-hydroxy methyl ester was the most dominant compound in all the four types of beans. These results are a first step towards addressing some of the occupational exposure associated with cooking Mucuna beans by rural communities.

Determination of cosmetic ingredients causing extrusion‐coated and adhesive joint multilayer packaging delamination

AbstractIn order to study the effect of several compounds on packaging stability, different cosmetic ingredients at two concentration levels were added to a NeoPCL® (Acofarma, Terrassa, Spain) water emulsion, and the preparations packed in sachets and stored at 40°C during 3 months. After that, the packaging was subjected to a T‐peel test and headspace solid‐phase microextraction gas chromatography mass spectrometry (HS‐SPME‐GC‐MS) analysis. The HS‐SPME‐GC‐MS analyses were performed using a 75 μm carboxen polydimethylsiloxane fibre to check for the presence of the studied analytes in the inner layers. The study revealed that the presence of a phenyl and a hydroxyl group in the compound structure lead to an important loss of adhesion between packaging layers joined by an adhesive. The interaction between the cosmetic ingredient and the adhesive was proposed as the main cause of the loss of adhesion. However, extrusion‐coating packaging was more susceptible to delamination, particularly with the volatile compounds. Copyright © 2009 John Wiley &amp; Sons, Ltd.