Carbosulfan Research Articles

The effect of paraquat dichloride and carbosulfan on soil conditions and population dynamic of soil microbes in a cornfield: a case study in Sumedang, West Java

Paraquat dichloride (PC) is one of the herbicides active ingredients that is widely used by farmers to control various types of weeds, while carbosulfan (CS) is one of the insecticide’s active ingredients that is widely used to control various types of insect pests. The objective of this study is to analyze the impact of pesticides for PC and CS on the chemical and physical conditions of the soil and the dynamics of fungi and soil bacteria population in a corn field in Sumedang district, West Java. The experiment took place between August and October, during the dry season, concluding just as the rainy season began. The PC concentrations used were 0 mL/L, 3.33 mL/L, 4 mL/L, 4.66 mL/L and 5.33 mL/L, while the CS concentrations used were 0 mL/L, 0.5 mL/L, 1 mL/L, 1.5 mL/L and 2 mL/L. Soil sampling was conducted before and after the application of pesticides. The total abundance of fungi and bacteria was analyzed using the total plate count (TPC) method. The application of PC to the cornfield slightly decreased soil bulk density from 1.354 g/cm3 to 0.816 g/cm3, while the application of CS slightly increased soil bulk density. A decrease in bulk density is considered positive for the physical conditions of the soil for plant cultivation. In general, the result indicated that the application of PC and CS did not show a significant effect on the chemical characteristics of the soil, and also it did not affect the population of fungi and bacteria. Weather conditions, i.e. dry season (June-September) and rainy season (started from October) seem to affect more on soil conditions as well soil microbial population than the application of the pesticides.

A new disposable electrochemical sensor for the individual and simultaneous determination of carbamate pesticides using a nanocomposite modified screen-printed electrode

In this work, we developed a disposable electrochemical sensor with improved sensitivity for the individual and simultaneous detection of carbaryl (CBR), fenobucarb (FNB), and carbosulfan (CBS) pesticides. The device used a screen-printed carbon electrode (SPCE) modified with a nanocomposite comprising manganese dioxide nanoparticles (MnO2) and graphene nanoplatelets (GNPs). We prepared the MnO2-GNPs nanocomposite by an in-situ growth method. The product was characterized by X-ray photoelectron spectrometry (XPS), X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The disposable sensor was fabricated by inhouse-printing using only carbon ink on inexpensive transparency paper. We investigated the electrochemical performance of the developed sensor by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). The MnO2 and GNPs nanocomposite shows the highest electrocatalytic activity toward the oxidation of carbamate when in its phenol form. Under the optimized experimental conditions, the proposed strategy possess a good linear response of the disposable sensor for the individual determination of CBR, FNB and CBS in the concentration range of 1-40 μM, 5-150 μM and 50-600 μM with the low detection limit of 0.30 μM, 1.30 μM, 14.90 μM, respectively. Besides, the analytical performance of the proposed sensor for the simultaneous detection of CBR, FNB, and CBS exhibits a linear response in ranges of 1–30 µM, 5–80 µM, and 50–400 µM, with the limit of detection being 0.30, 1.40, and 15.15 µM, respectively. In addition, the sensor displays high reproducibility, repeatability, and good selectivity. The electrode simultaneously applied to determine the three carbamate residues in Jasmine rice and rice-field water samples. This developed electrochemical sensor is a promising high-sensitivity tool for fast, easy-to-use, low-cost electrochemical detection of multi-pesticide residues in agricultural samples.

Computational chemistry study of toxicity of some m-tolyl acetate derivatives insecticides and molecular design of structurally related products.

Molecular descriptors (including quantum chemical, topological and physicochemical descriptors) were calculated for five m-tolyl derivatives insecticides [namely, carbosulfan (CBS), carbofuran (CBF), isoprocab (IFP), methiocarb (MTC) and isocarbophos (ICP)]. Calculated quantum chemical parameters included the total energy, the electronic energy, the binding energy, the core-core repulsion energy, the heat of formation, the dipole moment and the frontier molecular orbital energies. All the calculated quantum chemical parameters (except dipole moment) exhibited strong correlation with the experimental LD50 values of the studied insecticides (at various Hamiltonians). Calculated topological parameters included the molecular topological index (MTI), polar surface area (PSA), total connectivity (TC), total valence connectivity (TVC), Wiener index (WI), topological diameter (TD) and Balaban index (BI). However, only MTI, PSA, WI and BI exhibited excellent correlation with the toxicological activity of the insecticides. Also among all the calculated physicochemical parameters [logP, surface area (SA), surface volume (SV), hydration energy (EHydr), polarizability (PLZ) and refractivity (RFT)], only SV, EHydr, PLZ and RFT were useful in establishing quantitative structure activity relationship (QSAR). Application of QSAR indicated that the calculated theoretical LD50 values for the studied insecticides displayed excellent correlation with experimentally derived LD50 values. However, best results were obtained from quantum chemical descriptors under modified neglect of atomic overlap (MNDO). The toxicity profile of the insecticides also correlated strongly with ionization energy, electron affinity, global softness and global harness. Reactive sites of each of the insecticides were established using Fukui function, Huckel charges and HOMO/LUMO diagrams. Six new molecules were proposed and their theoretical activities were estimated. The proposed molecules included 2-methyl-2-(methylthio)-2,3-dihydrobenzofuran-7-yl methylcarbamate, O-methyl O-2-((methylaminooxy)carbonyl)phenyl phosphoramidothioate, 2-((methylaminooxy)carbonyl)phenyl methylcarbamate, 2-(1-(methylthio)ethyl)phenyl methylcarbamate, N-methyl-O-(2-(methylthiooxy) benzoyl) hydroxyl amine and 4-methyl naphthalen-2-yl methylcarbamate. Some of the proposed molecules exhibited negative values of LD50 (indicating extreme toxicity) while two of them exhibited values that are comparable to existing insecticides.

Different transformation of carbosulfan to its higher toxic metabolites in pakchoi (Brassica campestris ssp.) and cucumber (Cucumissativus L.) after field application

Carbosulfan (CAS) is used as an insecticide on vegetables, but its toxicity might increase due to the formation of higher toxic metabolites including carbofuran (CAN), 3-hydroxycarbofuran (3-OH) and 3-ketocarbofuran (3-KETO). In this study, the transformation of CAS to its metabolites in pakchoi (Brassica campestris ssp.) and cucumber (Cucumissativus L.) was investigated in the field in Beijing and Changsha and in a greenhouse in Hangzhou. CAS was metabolised to CAN, 3-OH and 3-KETO in pakchoi but only to CAN and 3-OH in cucumber. In pakchoi, the degradation of CAS (as measured by the DT50 value, the time marking the disappearance of 50% of the pesticide) was the fastest in Hangzhou (0.7 d), followed by Changsha (1.4 d) and Beijing (2.3 d), whereas in cucumber, its degradation was the fastest in Changsha (1.6 d) and Hangzhou (1.6 d), followed by Beijing (2.5 d). These results suggested the monitoring of metabolites should be a prior objective for CAS, and different metabolites should be considered when assessing the risk of CAS in different vegetables.

Anti-inflammatory Effects of N-acetylcysteine against Carbosulfan-induced Hepatic Impairment in Male Rats

The present study is designed to investigate the anti-inflammatory potential of N-acetylcysteine (NAC) against carbosulfan (CB)-induced hepatic dysfunction in rats. Rats exposed to CB and NAC were examined for toxicity by assessing various biochemical alteration, inflammation (interferon gamma and leucocyte counts), and peroxidation marker, including in liver. Significant increases of blood alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma glutamyltransferase (GGT) were detected in CB-treated rats. In addition, the level of pro-oxidative marker, such as malondialdehyde (MDA), was also increased. According to the results, rats exposed to CB showed a significant alteration in hepatic enzymes and an increase in the accumulation of lipid peroxidation index; also, CB induced an increase in pro-inflammatory cytokine in CB-treated rats when compared with their respective controls. Interestingly, administration of NAC to CB-treated rats attenuates the toxicity of CB, objectified by biochemical and oxidative improvement of liver. Thus, the present study reports for the first time that NAC could be a promising therapeutic agent against CB-induced liver impairment.

Protective Effect of N-Acetylcysteine Against Toxicity on the Rat Blood After Chronic Exposure to Carbosulfan

The present study investigated the protective effects of N-acetylcysteine (NAC), is widely known as an antidote to acetaminophen overdose, on carbosulfan (CB)-induced hematotoxicity and oxidative stress in male rats. CB was administered at a dose of 25 mg/kg or simultaneously administered with NAC (2 g/l) for 30 days. Results of hematological examination showed that red blood cells, hematocrite, hemoglobin, and reticulocytes levels were significantly lower in CB-exposed rats compared with those in the control. Administration of CB caused a significant increase in the superoxide dismutase and catalase activities. However, the glutathione (GSH) and thiols group (TSH) levels were significantly increased as well as GSH S-transferase activity and levels of glutathione peroxidase on erythrocytes of males rats compared with those in the control. Also, CB-treated rats showed significant elevation in lipid peroxidation (LPO) and acetylcholinesterase (AChE) on erythrocytes in comparison with the control. Co-administration with NAC exhibited chemoprotective effects against CB-mediated hematotoxicity, augmented erythrocyte antioxidant status, and prevented the induction of anemia.

Carbosulfan-induced oxidative damage following subchronic exposure and the protective effects of N-acetylcysteine in rats

Carbosulfan (CB)-induced oxidative stress leads to the inevitable accumulation of free radicals and eventual alteration of antioxidant enzymes in various biological systems. The present study is designed to investigate the preventive effect of N-acetylcysteine (NAC) on carbosulfan-induced hepatic and renal dysfunction in rats. Rats exposed to CB and NAC were examined for toxicity by assessing various biochemical alteration and stress markers including in liver and kidney. Significant increases of blood alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyltransferase (GGT), creatinine and urea were detected in CB-treated rats. In addition, the levels of antioxidative enzymes such as catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH) also were assessed. According to the results, rats exposed to carbosulfan showed a significant increase in the accumulation of stress markers and an alteration in the antioxidative enzymes activity, when compared to their respective controls. Interestingly, administration of NAC to CB-treated rats attenuates the toxicity of this compound, objectified by biochemical and oxidative improvement of liver and kidney. Thus, the present study reports for the first time that NAC could be a promising therapeutic agent against CB induced oxidative stress.

Sensitive Organophosphorus and Carbamate Pesticides Biosensor Based on Acetylcholinesterase and CdZnSe/ZnS Ternary Alloy Quantum Dots

This report presents the results of ternary alloy quantum dots (TQDs) manufacturing and its superior optical properties to fabricate the biosensor for pesticide detection. The explanation for the changes in PL intensity based on the change of electronic charges that alters the pH value of the surrounding environment, and the types of interaction on the surface of CdZnSe/ZnS TQDs were discussed. We show the decomposition process of the indicator (ATCh) with the catalysis from acetylcholinesterase enzyme (AChE) that inhibited by organophosphorus and carbamate pesticides including Trichlorfon (TF), Cypermethrin (CH), Abamectin (AT), and Carbosulfan (CS) with concentrations from 0.05 ppm to 10 ppm.

Immunosuppression and oxidative stress induced by subchronic exposure to carbosulfan in rat spleen: immunomodulatory and antioxidant role of N-acetylcysteine

The present study was designed to determine the immunosuppressive effects of carbosulfan (CB) and their relationship with an increased formation of reactive oxygen species in rat. Further, we aimed to evaluate the protective effects of N-acetyl-cysteine (NAC) against immunopathological changes induced by CB. Carbosulfan (25 mg/kg) and NAC (2 g/l) were given daily to rats during 30 days, via oral gavage and drinking water, respectively. Cell-mediated immune function, cytokines production, biomarkers of cell redox state maintenance, lipid peroxidation and the activities of antioxidant enzymes were measured in the spleen. Our data showed an increase in WBC percent (28.42%), a reduction in spleen CD8 T-lymphocytes (−85.63%) and a decrease in immunosuppressive cytokines production such as INF-gamma and IL-4. There was a switch from Th1-type to Th2-type cytokines with an unbalance toward anti-inflammatory cytokines. Moreover, a significant decrease in reduced glutathione (−71.68%) and total thiols (−39.81%) levels were observed in treated rats. Conversely, malondialdehyde level in spleen was increased (−42.3%), while glutathione-S-transferase, glutathione peroxidase, superoxide dismutase and catalase activities were depleted. Our results suggest that subchronic CB administration affects cellular enzyme and non-enzyme-mediated antioxidant defense systems and promotes immunotoxicity in rat. On the other hand, our data showed protective effects of NAC. Indeed, there was a recovery of oxidative stress markers and cytokines production. The use of NAC, in our study, as a therapeutic agent showed interesting results against CB toxicity.

Assessment of the Toxic Potential of Carbosulfan in Rats Following Subchronic Treatment

The effect of Carbosulfan (CB), a carbamate insecticide which has been used widely in agriculture, was investigated using wistar rat. Carbosulfan was administered orally at sublethal concentration at a dose of 25 mg/kg to 12 male rats ad libitum during the tests for 30 days consecutively. The effect of CB on hematological constituent (Red Blood Corpuscules (RBC), White Blood Corpuscules (WBC), Mean Cell Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), platelet (PLT) Hemoglobin (HGB) and Hematocrit (HCT) levels) and serum damage marker enzymes (aspartate aminotransferase (AST), alanin aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)) of rats were investigated under laboratory conditions .Body weight, organ weight, cellularity of spleen, spleen cell subtypes, cytokine production and lymphocyte proliferation assay were also studied. CB treatments caused different effects on the hematological constituents and the serum marker enzymes of the treatment groups as compared to the controls. Results showed that CB-treated group had a significantly lower RBC, HGB, HCT, MCV, MCH and MCHC and significantly higher WBC and PLT. Also, serum marker enzymes were affected by CB. For example, ALT, ALP, creatinine and urea significantly increased in rats treated with CB. Our data showed that CB at 25 mg/kg/day could suppress cellular activity of the immune system. At this dose, CB significantly decreased the proliferation response to PHA and the production of IFN gamma and IL-4.

Nematicides control rice root-knot, caused by Meloidogyne graminicola

Studies were conducted to determine damage potential of Meloidogyne graminicola on the commonly grown rice cv. Sugandh-5 and to devise an effective management strategy. The nematicides were applied through root-dip (200 ppm solution) and soil application of 2 kg ha-1 phorate 10G (25 mg a.i./pot), carbofuran 3G (83.3 mg a.i./pot and 1 L ha-1), carbosulfan 20EC (5µL/pot) and chlorpyriphos 20 EC (6.25 µL/pot) in both nematode infested and non-infested soil with five modes of application viz., root-dip, single soil application (15 days after transplanting), root-dip + one soil application, two soil applications, and root-dip + two soil applications (15 and 30 days). Application of nematicides did not cause any toxicity symptoms on rice plants. In nematode infested soil, terminal and spiral galls developed on the rice roots, and plants suffered 20−31% decrease in the plant growth parameters. Carbofuran and phorate through root-dip plus single soil application provided greatest suppression in galling (16−20%), egg mass production (18−22%) and soil population (27.5−58.2%) of M. graminicola , and subsequently increased all the plant growth variables by 9−19%. Root-dip + two soil applications increased plant growth and suppressed nematodes, but was equal to root dip + one soil application. Root-dip treatment alone with carbosulfan also significantly suppressed root galling (10−12%) and improved the dry weight of roots and shoots (7−10%).

Simultaneous quantification of carbamate insecticides in human plasma by liquid chromatography/tandem mass spectrometry

Carbofuran (CFN), carbosulfan (CSN) and fenobucarb (FBC) are carbamate pesticides that are widely used in gardening and agriculture for the control of insects. Human poisoning due to occupational or self-poisoning exposures is also reported, so assays are required to quantify the plasma concentration of these insecticides. An LC–MS/MS method was developed and validated for the simultaneous quantification of these three carbamate insecticides in the plasma of patients with acute intentional self-poisoning. Plasma samples were pretreated by acetonitrile for protein precipitation. Chromatography was carried out on a Luna C18(2) analytical column with gradient elution using a mobile phase containing acetonitrile and water with 10 mM ammonium acetate. Mass spectrometric analysis was performed by an Applied Biosystems MDS Sciex API 2000 triple quadrupole mass spectrometer coupled with electrospray ionization (ESI) source in the positive ion mode. The total run time was 7 min. The assay was validated over a concentration range from 10 to 1000 ng/ml for CSN and FBC and 20–2000 ng/ml for CFN. The precision and accuracy for both intra- and inter-day determination of all analytes were acceptable (<15%). No significant matrix effect was observed. Stability of compounds was established for short term bench and autosampler storage as well as freeze/thaw cycles. The method was effectively applied to 270 clinical samples from patients with a history of acute intentional carbamate self-poisoning.

A carbosulfan insecticide to protect pine seedlings from &lt;i&gt;Hylastes ater&lt;/i&gt; (Coleoptera Scolytidae) damage

A controlled release granular carbosulfan pesticide was tested for efficacy against Hylastes ater damage of Pinus radiata seedlings in secondrotation forests in the central North Island Our study involved control seedlings and seedlings treated with 10 g or 15 g carbosulfan granules per seedling Seedling mortality ranged from 0 to 8 at the five sites When seedling mortality was high (8) mortality was reduced to 07 and 0 for carbosulfan granules applied at 10 g/seedling and 15 g/seedling respectively While seedling mortality in the five sites was low a large proportion of seedlings were damaged (38 to 83 of untreated seedlings) by H ater Untreated seedlings were damaged more frequently and with greater severity than treated seedlings The results indicate that that carbosulfan should protect P radiata seedlings from damage caused by H ater when used at the recommended field rate