Carbonyl Products Research Articles

Household Ozone Disinfector as An Alternative Ozone Generator for Ozonolysis of Alkenes

AbstractOzonolysis is an oxidative cleavage reaction of alkenes with ozone that is indispensable and has found many applications in organic synthesis. However, dedicated laboratory ozone generators are quite expensive equipment and may not be affordable by many laboratories. Recently, low‐cost ozone disinfectors have become more common as a household appliance. Herein, we demonstrate the applicability of a household ozone disinfector, which can produce ozone in the order of 10 mmol/h, as alternative equipment for the laboratory scale ozonolysis of alkenes. The ozonolysis of 14 styrene derivatives bearing electron‐donating and electron‐withdrawing groups on aromatic rings including those with α‐ and β‐substituents, as well as 8 natural products containing olefinic moieties were investigated. Ozonolysis of these alkenes at millimole scales proceeded to completion within 30–40 minutes and the carbonyl products were obtained in 68% to quantitative yields. Preparative‐scale ozonolysis of 4‐methoxystyrene (6.17 g, 46 mmol) was also demonstrated. The reaction proceeded smoothly to yield 4‐anisaldehyde in 92% yield, which was subsequently converted to p‐anisaldehyde dimethyl acetal in 66% yield. This work presents household ozone disinfector as an affordable, compact size, and practical synthetic equipment that does not require extra modification. Thus, the household ozone disinfector offers convenient access to ozonolysis or other reactions involving ozone for low‐budget laboratories.

The level of carbonylation of plasma proteins and peripheral blood leukocytes in patients with different duration of Alzheimer’s disease

The course of Alzheimer’s disease is associated with an increase in oxidative stress associated with an increase in the production of reactive oxygen species against the background of neurodegenerative inflammation, and a simultaneous depletion of the antioxidant defense capabilities of brain cells. The result is the oxidative modification of macromolecules: proteins, lipids, nucleic acids. Protein carbonylation products accumulate not only in neurons, and in direct correlation with the degree of increase in amyloidosis and neurodegeneration, but also in extra-neuronal tissues, including leukocytes. In the course of this study, the levels of spontaneous and induced oxidative modification of proteins were determined in the blood plasma and fractionated leukocytes of peripheral blood of patients with different durations of Alzheimer’s disease, and the value of the reserve-adaptive potential was assessed as markers of the severity of oxidative stress. It has been established that the course of Alzheimer’s disease has a greater effect on the accumulation of carbonyl derivatives in blood plasma. In patients with a disease duration of 5–10 years, the total level of aldehyde and ketone derivatives of modified plasma proteins exceeds the same indicator in subgroups with a shorter duration of the disease. This tendency is less typical for mononuclear leukocytes. The level of induced oxidative modification of proteins increases to a greater extent in blood plasma than in fractionated leukocytes. This indicates the depletion of the reserve-adaptive potential of plasma antioxidant capabilities, which is more pronounced in patients with a long course of Alzheimer’s disease. For polymorphonuclear leukocytes, such a pattern was not revealed, which is probably associated with a short cell life. In mononuclear leukocytes, as well as in plasma, there is a tendency to depletion of the reserve-adaptive potential, but to a lesser extent.

The Prophylactic Activity of Punica granatum L. mesocarp Protects Preadipocytes against Ribosylated BSA-Induced Toxicity

Objective It was aimed at comparing the glycating capacities of glucose and ribose in bovine serum albumin (BSA) and anti-glycation activity of pomegranate mesocarp extract (PME). The protective mechanism of PME against ribosylated BSA (BSARIB)-induced toxicity was also investigated. Methods BSA was incubated with glucose or ribose in the presence or absence of PME for 15 days. In preadipocytes pretreated with PME, cell viability, ROS production, lipid peroxidation and mitochondrial membrane potential were investigated following 1, 6, 12, 18 and 24 h exposure to BSARIB. Nuclear translocation of NFκB was assessed at 1 h and 24 h of BSARIB insult. Accumulation of oxidized proteins, activities of intrinsic antioxidant enzymes and IL-6 secretion were also determined after 24 h exposure to BSARIB. Results Ribose was a harsher glycating agent as compared to glucose and PME showed strong anti-glycation activity by suppressing (P < 0.05) the increase in levels of fluorescent AGEs, Amadori products, protein carbonyl and advanced oxidation protein products (AOPP). In preadipocytes, BSARIB potentiated pro-apoptotic activity by inhibiting the nuclear translocation of NFκB. BSARIB induced a time dependent decrease in cell viability, which was significantly suppressed (P < 0.05) by PME. The extract also significantly reduced (P < 0.05) the time dependent increase in ROS level and associated lipid peroxidation as well as loss in mitochondrial membrane potential caused by BSARIB. PME also counteracted the BSARIB-induced accumulation of oxidized proteins, decrease in intrinsic antioxidant activity and IL-6 over-secretion. Conclusions PME showed anti-glycation activity and afforded protection against BSARIB-induced toxicity, oxidative stress and inflammation in preadipocytes.

The dynamics of indicators of peroxide metabolism in patients with cicatricial skin deformations in combination with metabolic syndrome in complex use of physiotherapeutic treatment methods and drinking mineral waters

The article analyzes the role of lipid peroxidation processes in the pathogenesis of cicatricial skin changes, including in patients with metabolic syndrome. Baseline indices of LPO processes and antioxidant enzymes in patients with metabolic syndrome indicate the development of oxidative stress and accumulation of cytotoxic carbonyl products. The course use of a combination of fermenkol phonophoresis, fractional photothermolysis and mineral water «Essentuki №17» in patients with cicatricial changes in the skin and metabolic syndrome is accompanied by a decrease in the severity of insulin resistance and lipid peroxidation processes. The use of a complex of physiotherapeutic factors and drinking mineral water contributes to an increase in the coefficient of antioxidant protection by 54%. The revealed dynamics of the assessed parameters is realized due to the elimination of molecular inhibitors of antioxidant enzymes in conditions of an increase in the receptor sensitivity of tissues to insulin and a decrease in the level of glycemia. The manifestation of antioxidant activity in the combined use of physiotherapeutic methods and mineral waters for drinking purposes allows this technology to be considered as an effective means of pathogenetic therapy of cicatricial deformities of the skin in combination with metabolic syndrome.

Plasma from Young Rats Injected into Old Rats Induce Antiaging Effects

An experimental novel antiaging intervention strategy is based on the concept of parabiosis, which involves long-term treatment with factors derived from young blood facilitating rejuvenation of old individuals. In this study, we employed blood plasma from young rats as an intervention strategy to evaluate whether this could impact aging biomarkers in aged rats. The biomarkers studied include: reactive oxygen species, the ferric reducing ability of plasma, plasma membrane redox system, reduced glutathione, malondialdehyde, protein carbonyl, and advanced oxidation protein products in blood. Additionally, the level of tumor necrosis factor-α and interleukin-6 were also estimated in blood. We found that old rats injected with plasma from young rats were protected from oxidative stress. Thus, this study provides some evidence of the rejuvenating effects of young plasma. We hypothesize that young plasma may contain certain "factors," which may be responsible for the observed effects. The mechanism of action is not clearly understood and is open to further studies.

A new analytical model for predicting the radio-thermal oxidation kinetics and the lifetime of electric cable insulation in nuclear power plants. application to silane cross-linked polyethylene

The radio-thermal oxidation of silane cross-linked polyethylene (Si-XLPE) was studied in air under different γ dose rates (6.0, 8.5, 77.8, and 400 Gy.h−1) at different temperatures (21, 47, and 86°C). The changes in the physico-chemical and mechanical properties of Si-XLPE throughout its exposure were determined by FTIR spectroscopy, differential scanning calorimetry (DSC), swelling measurements, rheometry in rubbery (DMTA) and in molten states, and uniaxial tensile testing. It was found that oxidation leads to the build-up of a wide variety of carbonyl and hydroxyl products (mostly carboxylic acids and hydroperoxides) and an efficient chain scission process that catastrophically reduces the concentration in elastically active chains and the elongation at break from the early periods of exposure. A new analytical model was derived from the current radio-thermal mechanistic scheme without making the usual assumption of thermal stability of hydroperoxides. After an initial period where the oxidation kinetics occurs with a constant rate, this model allows also predicting the auto-acceleration of the oxidation kinetics when the hydroperoxide concentration reaches a critical value of about 1.6 × 10−1 mol.L−1. Choosing this critical value as a structural end-of-life criterion allows a more direct assessment of the lifetime of Si-XLPE in the various radio-thermal environments under study, except at the highest temperature (i.e. 86°C) where the kinetic model can still be noticeably improved.

Z-Selective phosphine promoted 1,4-reduction of ynoates and propynoic amides in the presence of water.

Phosphine-mediated reductions of substituted propynoic esters and amides in the presence of water yield the partially reduced α,β-unsaturated esters and amides with high Z-selectivity. The competitive in situ Z to E-isomerization of the product in some cases lowers the Z to E ratios of the isolated α,β-unsaturated carbonyl products. Reaction time and the amounts of phosphine and water in the reaction mixture are the key experimental factors which control the selectivity by preventing or reducing the rates of Z- to E-product isomerization. Close reaction monitoring enables isolation of the Z-alkenes with high selectivities. The computational results suggest that the reactions could be highly Z-selective owing to the stereoselective formation of the E-P-hydroxyphosphorane intermediate.

Оценка стабильности смазывающей способности дизельных топлив при их искусственном окислении и опытном хранении

The paper is given to investigation and definition of optimal parameters of oxidation of diesel fuels for the purpose of estimation its lubrication stability under the storage conditions. On the foundation of critical analyze of previous method’s parameters it were corrected the volume of oxidating fuel, the type of catalyze-active metal, which is contacting the fuel and also the fuel-metal and fuel-air contact-square. These corrections are allowed to increase the adequacy of chemical composition changes of diesel fuel at the oxidation to the same changes at the storage conditions. It was found that using the cuprum as the catalyze-active material of storage tanks increases the appearance of carbonyl and carboxyl oxidation products and decreases the appearance of hydroxyl oxidation products that is not adequately the chemical changes of diesel fuels while its storage in steel tare. On the foundation of three samples of diesel fuel investigation was found that decreasing of lubrication ability of diesel fuel at its storage for the 10 months could be forecasted by its oxidation via the developed method at least of 2, 4 and 6 hours with the following estimation lubrication ability of oxidated samples.

Probing glycation potential of dietary sugars in human blood by an integrated in vitro approach

Glycation is referred to as the interaction of protein amino and guanidino groups with reducing sugars and carbonyl products of their degradation. Resulting advanced glycation end-products (AGEs) contribute to pathogenesis of diabetes mellitus and neurodegenerative disorders. Upon their intestinal absorption, dietary sugars and α-dicarbonyl compounds interact with blood proteins yielding AGEs. Although the differences in glycation potential of monosaccharides are well characterized, the underlying mechanisms are poorly understood. To address this question, d-glucose, d-fructose and l-ascorbic acid were incubated with human serum albumin (HSA). The sugars and α-dicarbonyl intermediates of their degradation were analyzed in parallel to protein glycation patterns (exemplified with hydroimidazolone modifications of arginine residues and products of their hydrolysis) by bottom-up proteomics and computational chemistry. Glycation of HSA with sugars revealed 9 glyoxal- and 14 methylglyoxal-derived modification sites. Their dynamics was sugar-specific and depended on concentrations of α-dicarbonyls, their formation kinetics, and presence of stabilizing residues in close proximity to the glycation sites.

Copper-Catalyzed Formal Dehydrogenative Coupling of Carbonyls with Polyfluoroarenes Leading to β-C-H Arylation.

We herein communicate a formal dehydrogenative coupling of carbonyls with polyfluoroarenes enabled by Cu catalysis. Silyl enol ethers initially prepared from carbonyls are postulated to undergo the copper-mediated oxidative dehydrogenative coupling with polyfluoroarenes via a radical pathway. Including cyclic and linear ketones, aldehydes, and esters, a broad range of β-aryl carbonyl products were efficiently obtained in high regio- and stereoselectivity with excellent functional group tolerance.

Selective dehydrogenation of isopropanol on carbonized metal–organic frameworks

Alcohol dehydrogenation to carbonyl compounds and hydrogen (H2) gas offers a green and sustainable method for producing H2 and the synthesis of various pharmaceuticals and fine chemicals. However, it is a challenge to acquire remarkable selectivity toward carbonyl products. Herein, CuO@C catalyst displays high dehydrogenation over dehydration reaction for isopropanol. CuO@C core–shell was synthesized from the carbonization of metal–organic frameworks (MOFs) via a single step without the need for incorporating an external source of carbon. CuO@C showed high selectivity (100%) and increased conversion (100%) of isopropanol to acetone and hydrogen via dehydrogenation. It exhibited high stability under the reactant flow for a long time and reusability for several cycles. A mechanism of the dehydrogenation was also highlighted using different analytical methods, including X-ray diffraction (XRD), electron diffraction (ED), Fourier transform infrared (FT-IR), and high-resolution transmission electron microscopy (HR-TEM).

Sweet cherry (Prunus avium) fibers extracted from microwave- and steam-blanched recovered fruits: Photo-antioxidant activity in milk proteins

Sweet cherries (Prunus avium L.) discarded at harvesting were upgraded as powerful antioxidant fiber powders for food preservation, and their photo-antioxidant effect on milk proteins evaluated. To stabilize the powders during their processing and use, cherries were first blanched through microwaves or saturated water vapor for comparison. The blanching condition (determined as that required to achieve enough peroxidase (POX) inactivation) was 6 min and 90 °C in the fruit center, and POX was the enzyme selected as indicator of blanching efficiency because it was more resistant than polyphenoloxidase in both processes. Fibers, the water insoluble residues, were then separately extracted from the microwave- or steam-blanched cherries. The fibers obtained after freeze-drying exhibited good hydration properties, as they were essentially constituted by cell wall biopolymers (uronic acids, neutral sugars, cellulose, lignin, proteins). Phenolic, mainly proanthocyanidins (≈600 mg/100 g fiber), were co-extracted together with lower amounts of extractable phenolics (≈120 mg/100 g), mainly cyanidin-3-(6′-p-coumaroyl) glucoside (dark violet color). Powders showed high 2,2-diphenyl-1-picrylhydrazyl radical scavenging (≈485 mg/100 g fiber) and ferric reducing antioxidant power (835-674 mg/100 g) capabilities (expressed as ascorbic acid). In whole milk, independently of the blanching process used, the fiber powders obtained exerted almost the same concentration (0–9.0 g fiber/100 mL milk)-dependent protective effect against protein photo-oxidation under UV-C radiation (200–280 nm), measured as carbonyl production. We concluded that the antioxidant power of the cherry fibers was mainly due to phenolics. These powders may be useful for the food industry as natural additives for functional antioxidant preservation of milk protein products.

Impact of Atomizer Age and Flavor on In Vitro Toxicity of Aerosols from a Third-Generation Electronic Cigarette against Human Oral Cells.

Electronic cigarettes (ECs) are categorized into generations which differ in terms of design, aerosol production, and customizability. Current and former smokers prefer third-generation devices that satisfy tobacco cravings more effectively than older generations. Recent studies indicate that EC aerosols from first- and second-generation devices contain reactive carbonyls and free radicals and can cause in vitro cytotoxicity. Third-generation ECs have not been adequately studied. Further, previous studies have focused on cells from the respiratory tract, whereas those of the oral cavity, which is exposed to high levels of EC aerosols, have been understudied. We quantified the production of reactive carbonyls and free radicals by a third-generation EC and investigated the induction of cytotoxicity and oxidative stress in normal and cancerous human oral cell lines using a panel of eight commercial EC liquids. We found that EC aerosols produced using a new atomizer contained formaldehyde, acetaldehyde, and acrolein, but did not contain detectable levels of free radicals. We found that EC aerosols generated from only one of the eight liquids tested using a new atomizer induced cytotoxicity against two human oral cells in vitro. Treatment of oral cells with the cytotoxic EC aerosol caused a concomitant increase in intracellular oxidative stress. As atomizer age increased with repeated use of the same atomizer, carbonyl production, radical emissions, and cytotoxicity increased. Overall, our results suggest that third-generation ECs may cause adverse effects in the oral cavity and normal EC use, which involves repeated use of the same atomizer to generate aerosol, may enhance the potential toxic effects of third-generation ECs.

Storage stability of soy protein isolate powders containing soluble protein aggregates formed at varying pH.

Soy protein is wildly used in food industry due to its high nutritional value and good functionalities. However, the poor storage stability of commercial soy protein products has puzzled both the producers and the users for a long time. The current study assessed the changes in protein solubility, aggregation, oxidation, and conformation of soy protein isolate (SPI) with various soluble aggregates formed at different pH values (pH 5–8) during storage. During storage, SPI samples showed a reduced protein solubility (p < .05), an increased protein oxidation (p < .05), and an attenuated conformational enthalpy (∆H). SPI with a higher pH produced more disulfide‐mediated aggregates at the expense of sulfhydryl groups and experienced greater losses of protein tertiary structure and a faster reduction in solubility. Yet, all samples nearly shared similar rising trend during 8‐week storage, which indicated the production of protein carbonyls was insensitive to pH. Soluble aggregates present in fresh SPI samples appeared to induce instability of SPI during storage. These findings suggested SPI prepared at pH 6 was in favor of its storage stability, and soluble aggregates presented in fresh samples should be paid more attention for further study of storage stability kinetics.

Zika virus induces oxidative stress and decreases antioxidant enzyme activities in vitro and in vivo

The first outbreak of Zika virus (ZIKV) infection in the Americas, especially in Brazil, was reported in 2015. Fever, headache, rash, and conjunctivitis are the common symptoms of ZIKV infection. Unexpected clinical outcomes, such as microcephaly and Guillain-Barré syndrome, have also been reported. The recent spread of ZIKV and its association with severe illness has created an urgent need to understand its pathogenesis and find potential therapeutic targets. Studies show that some viruses, including Flavivirus, trigger oxidative stress, which affects cellular metabolism, viral cycle, and pathogenesis. However, the role of oxidative stress in ZIKV infection needs to be investigated. Here, we analyzed ZIKV infection-triggered oxidative stress and modified antioxidant enzyme activities. U87-MG and HepG2 cells were infected to measure reactive oxygen species (ROS), malondialdehyde (MDA), and carbonyl protein levels, the activities of superoxide dismutase (SOD) and catalase (CAT), and the activation of nuclear factor erythroid 2p45-related factor 2 (Nrf2). ZIKV infection induced a significant increase in ROS, lipid peroxidation, and protein carbonylation products and a significant decrease in SOD and CAT activities accompanied by inhibition of Nrf2 activation in both cell lines. Further, MDA and carbonyl protein levels and SOD and CAT activities were evaluated in the brain and liver of ZIKV-infected C57BL/6 mice, and oxidative stress associated with antioxidant depletion was also found to occur in vivo. Together, our findings indicate the potential use of antioxidants as a novel therapeutic approach to Zika disease, and future studies in this direction are warranted.

Thermal aging of High Tenacity Polyvinyl Alcohol yarns

A both multiscale and multi-techniques approach was developed to characterize the thermal aging in air at 70, 90, 100 and 120 °C of High Tenacity Polyvinyl Alcohol (HT-PVAl) yarns, and to elucidate the consequences of thermal aging on the chemical structure, crystalline morphology and several physico-chemical and mechanical properties of practical interest. First of all, FTIR and UV–Vis spectrometries showed that the main degradation products are isolated (1656 cm−1) and conjugated (1592 cm−1) carbon-carbon double bonds, and a large variety of carbonyl products such as: esters (1757 cm−1), aldehydes (1737 cm−1), carboxylic acids (1720 cm−1), ketones (1707 cm−1), but also polyenones (1630 cm−1). Possible mechanistic schemes were then proposed to explain the formation of all these different degradation products. Moreover, no change in the physico-chemical properties of the amorphous phase was detected by modulated differential scanning calorimetry and dynamic vapor sorption. In contrast, the decrease in both melting temperature and crystallinity ratio with exposure time clearly indicates a “nibbling” of the crystalline phase during thermal aging, thus highlighting a chemical attack preferentially localized at the amorphous/crystal interface for this complex anisotropic material. This heterogeneous degradation is responsible for the loss of fracture properties in tension of HT-PVAl yarns. Fairly simple relationships between the structural variables determined at the different scales of analysis were established. As an example, a linear correlation was found between the changes in fracture properties and the changes in crystalline morphology.

The Structural Integrity of the Model Lipid Membrane during Induced Lipid Peroxidation: The Role of Flavonols in the Inhibition of Lipid Peroxidation.

The structural integrity, elasticity, and fluidity of lipid membranes are critical for cellular activities such as communication between cells, exocytosis, and endocytosis. Unsaturated lipids, the main components of biological membranes, are particularly susceptible to the oxidative attack of reactive oxygen species. The peroxidation of unsaturated lipids, in our case 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), induces the structural reorganization of the membrane. We have employed a multi-technique approach to analyze typical properties of lipid bilayers, i.e., roughness, thickness, elasticity, and fluidity. We compared the alteration of the membrane properties upon initiated lipid peroxidation and examined the ability of flavonols, namely quercetin (QUE), myricetin (MCE), and myricitrin (MCI) at different molar fractions, to inhibit this change. Using Mass Spectrometry (MS) and Fourier Transform Infrared Spectroscopy (FTIR), we identified various carbonyl products and examined the extent of the reaction. From Atomic Force Microscopy (AFM), Force Spectroscopy (FS), Small Angle X-Ray Scattering (SAXS), and Electron Paramagnetic Resonance (EPR) experiments, we concluded that the membranes with inserted flavonols exhibit resistance against the structural changes induced by the oxidative attack, which is a finding with multiple biological implications. Our approach reveals the interplay between the flavonol molecular structure and the crucial membrane properties under oxidative attack and provides insight into the pathophysiology of cellular oxidative injury.

Synthesis of α-Trifluoromethylthio-α,β-Unsaturated Carbonyl Compounds by DABCO-Mediated Electrophilic Trifluoromethylthiolation with N-SCF3-Dibenzenesulfonimide

A DABCO-mediated electrophilic α-trifluoromethylthiolation of α,β-unsaturated carbonyl compounds comprising no β-substituents has been achieved using N-trifluoromethylthio-dibenzenesulfonimide as the SCF3 source. The direct trifluoromethylthiolation provides the corresponding α-trifluoromethylthio-α,β-unsaturated carbonyl products in good yields (up to 88%). Furthermore, the vinyl group in the α-trifluoromethylthio-α,β-unsaturated carbonyl product was successfully transformed into diverse functional groups in good to excellent yields (70-95%) by reactions such as epoxidation, aziridination, hydrocyanation, and hydrogenation.

Experimental Evidence of Dioxole Unimolecular Decay Pathway for Isoprene-Derived Criegee Intermediates.

Ozonolysis of isoprene, one of the most abundant volatile organic compounds emitted into the Earth's atmosphere, generates two four-carbon unsaturated Criegee intermediates, methyl vinyl ketone oxide (MVK-oxide) and methacrolein oxide (MACR-oxide). The extended conjugation between the vinyl substituent and carbonyl oxide groups of these Criegee intermediates facilitates rapid electrocyclic ring closures that form five-membered cyclic peroxides, known as dioxoles. This study reports the first experimental evidence of this novel decay pathway, which is predicted to be the dominant atmospheric sink for specific conformational forms of MVK-oxide (anti) and MACR-oxide (syn) with the vinyl substituent adjacent to the terminal O atom. The resulting dioxoles are predicted to undergo rapid unimolecular decay to oxygenated hydrocarbon radical products, including acetyl, vinoxy, formyl, and 2-methylvinoxy radicals. In the presence of O2, these radicals rapidly react to form peroxy radicals (ROO), which quickly decay via carbon-centered radical intermediates (QOOH) to stable carbonyl products that were identified in this work. The carbonyl products were detected under thermal conditions (298 K, 10 Torr He) using multiplexed photoionization mass spectrometry (MPIMS). The main products (and associated relative abundances) originating from unimolecular decay of anti-MVK-oxide and subsequent reaction with O2 are formaldehyde (88 ± 5%), ketene (9 ± 1%), and glyoxal (3 ± 1%). Those identified from the unimolecular decay of syn-MACR-oxide and subsequent reaction with O2 are acetaldehyde (37 ± 7%), vinyl alcohol (9 ± 1%), methylketene (2 ± 1%), and acrolein (52 ± 5%). In addition to the stable carbonyl products, the secondary peroxy chemistry also generates OH or HO2 radical coproducts.

Protective effects of Avena sativa against oxidative stress-induced kidney damage resulting from an estrogen deficiency in ovariectomized Swiss mice model.

The aim of this study was to evaluate the antioxidant capacity of Avena sativa and to confirm its protective effects on estrogen deficiency in ovariectomized mice (OVX) model. Animals were treated during 21 and 60days as follows: negative control, positive control treated with oat, ovariectomized, and ovariectomized receiving hormonal or oat treatments, respectively. 21days post-surgery, our results showed that ovariectomy increases weight gain and urea level in plasma. After 60days of treatment, OVX showed a gain in weight, high increases in relative kidney weight, plasmatic creatinine, glutathione peroxidase (GPX) activity, and protein oxidation as shown by elevated protein carbonyl (PCO) and advanced oxidation protein products (AOPP) levels. On the other side, a decrease was detected in catalase activity. Meanwhile, no significant changes in urea level, lipid peroxidation, SOD and GSH activity were detected. Conversely, supplementation with oat reduced weight gain, as well as oxidative stress and oxidation protein in the kidney of OVX mice. PRACTICAL APPLICATIONS: In our study, we removed the two ovaries of adult female mice in order to eliminate the essential source of steroid hormones. Deficiency in these sexual hormones, especially estrogen, leads to several physiological and morphological disturbances that are found in post-menopausal women. The gain in weight and oxidative kidney damages were the main health problems detected in ovariectomized animals. This work explored for the first time the use of oat grains as a safe natural remedy to treat all previous health problems and to avoid carcinogenic effects of the menopausal hormone therapy. Owing to its health benefits, this cereal species has recently gained attention as an important source of antioxidants and fibers. Thanks to these two components, it can be able to restore all indicated disturbances. Therefore, this cereal can be very useful in the prevention of obesity and kidney oxidative damage occurring during menopause.