Carbon Burial Research Articles

The onset of the major glaciation of the LPIA: record from South China

The beginning of the Late Paleozoic Ice Age was characterized by localized events with small ice depocenters occurring in South America and a series of cyclic shallow-water limestones in far-field regions, in late Visean to Serpukhovian. This paper documents two topmost Visean–Serpukhovian sections in Guangxi, South China: a stromatolite-bearing deep-water succession in Helv village and a shallow-water succession in Dujie village. Seventeen microfacies types have been recognized and grouped into six microfacies associations. The vertical evolution of the microfacies associations from deep-water to shallow-water depositional environments in both sections indicate a major relative sea-level fall in the latest Visean. The repetitive successions comprising different morphological stromatolites in Helv section and the cyclic alternation of subtidal and peritidal facies in Dujie section imply high-frequency sea-level fluctuations during latest Visean to Serpukhovian, most likely eustatic in origin. The carbon isotope data of Dujie limestone exhibit a pronounced positive δ13C excursion with ± 4.3‰ in the peritidal deposits. This shift is interpreted as the result of enhanced organic carbon burial in other regions and depletion of the ocean in light carbon. The widespread coeval cyclicity occurring in settings in South China, Western Europe, and North America is most likely the results of glacio-eustatic sea-level fluctuations, possibly with local influences of tectonic movements. This pattern is interpreted to represent the expression of the onset of the major glaciation during the LPIA in low-latitudinal successions.

Paleoenvironmental setting, mechanism and consequence of massive organic carbon burial in the Permian Junggar Basin, NW China

The Junggar Basin in northwestern China is a typical “walled” sedimentary basin that has a prolonged basin evolution history from the Paleozoic to Cenozoic and is rich in petroleum resources. Permian strata reach thousands of meters in thickness and have been intensively studied for their record of regional tectonics and paleoenvironments. They have also been exploited for oil and gas for the past several decades. In this paper, we summarize perspectives on tectonic evolution, stratigraphic framework, sedimentary environments, and the paleoclimate of the Permian Junggar Basin and the adjacent Turpan Basin. Further, we discuss the mechanism and consequences of massive organic carbon burial. Based on a compilation of published radiometric dating datasets, an updated chronostratigraphic framework was proposed for the Junggar Basin. This framework constrains the ages of two main petroleum source intervals, the Fengcheng Formation and the Lucaogou and equivalent formations, as Early Permian and Middle Permian, respectively. Although a global warming and drying trend was present throughout the Permian Period, the regional climate over the Junggar Basin was drier in Early Permian and more humid in the Middle-Late Permian with short-term climatic fluctuations. During the deposition of the two petroleum source intervals, dry and temperate climate prevailed and evaporative lacustrine environment dominated. Organic matter enrichment was promoted by stable salinity stratification and limited dilution by inorganic sediments, leading to one of the richest lacustrine source rock deposits in the world. The average organic carbon burial rates were 11.2 gC/m2/yr for the Lucaogou Formation and 3.5 gC/m2/yr for the Fengcheng Formation, lower than the average organic carbon burial rate in modern lakes. However, considering the large areas of global tectonic lakes in the Permian Period, organic carbon burial in lacustrine environments could act as a significant carbon sink and impact the global carbon cycle and climate evolution.

Organic carbon burial and sources in soils of coastal mudflat and mangrove ecosystems

Mangrove organic carbon is primarily stored in soils, which contain more than two-thirds of total mangrove ecosystem carbon stocks. Despite increasing recognition of the critical role of mangrove ecosystems for climate change mitigation, there is limited understanding of soil organic carbon sequestration mechanisms in undisturbed low-latitude mangroves, specifically on organic carbon burial rates and sources. This study assessed soil organic carbon burial rates, sources and stocks across an undisturbed coastal mudflat and mangrove hydrogeomorphological catena (fringe mangrove and interior mangrove) in Bintuni Bay, West Papua Province, Indonesia. 210Pb radionuclide sediment dating, and mixing model of natural stable isotope signatures (δ 13C and δ15N) and C/N ratio were used to estimate organic carbon burial rates and to quantify proportions of allochthonous (i.e., upland terrestrial forest) and autochthonous (i.e., on-site mangrove forest) organic carbon in the top 50 cm of the soil. Burial rates were in the range of 0.21–1.19 Mg C ha−1 yr−1. Compared to the fringe mangroves, organic carbon burial rates in interior mangroves were almost twice as high. Primary productivity of C3 upland forest vegetation and mangroves induced soil organic carbon burial in interior mangroves and this was consistent with the formation of the largest organic carbon stocks (179 ± 82 Mg C ha−1). By contrast, organic carbon stored in the fringe mangrove (68 ± 11 Mg C ha−1) and mudflat (62 ± 10 Mg C ha−1) soils mainly originated from upland forests (allochthonous origin). These findings clearly indicate that carbon sequestered and cycling in mangrove and terrestrial forest ecosystems are closely linked, and at least a part of carbon losses (e.g., erosion) from terrestrial forests is buried in mangrove ecosystems.

Darriwilian carbon isotope stratigraphy in the Taebaeksan Basin, Korea and its implications for Middle Ordovician paleoceanography

The middle Darriwilian carbon isotope excursion (MDICE) event is first documented from central eastern Korea (Taebaeksan Basin), the eastern part of the Sino-Korean Block, and is well correlated with global carbon isotope chemostratigraphy. The Korean MDICE record shows three broad positive peaks and complements records from the Middle Ordovician peri-Gondwanan epeiric seas showing incomplete records due to disconformity. This study reconstructed paleoceanographic conditions with nitrogen isotopic compositions and clay mineral compositions to understand the causes of carbon isotope excursions. The heavier excursion in nitrogen isotopic curve and abrupt increase of kaolinite in the early MDICE interval are interpreted as a result of epeiric sea denitrification associated with strong seawater stratification, and the main cause of those conditions was likely increased precipitation in the adjacent land. Subsequent sea-level rise caused an anti-estuarine circulation in the Taebaeksan sea and increased the organic carbon burial in the adjacent basin setting, which might have sustained the MDICE. The documentation of the MDICE event in this study supports the view that the MDICE occurred globally. This study provides not only information on the regional paleoceanographic conditions that occurred in the Middle Ordovician epeiric seas during the course of MDICE, but also reports that the MDICE event occurred as a response to seawater circulation associated with global sea-level rising in the Middle Ordovician.

Climate and environmental response to the break-up of Pangea during the Early Jurassic (Hettangian-Pliensbachian); the Dorset coast (UK) revisited

Abstract Between the end-Triassic mass extinction and the Toarcian Oceanic Anoxic Event, the Early Jurassic witnessed important changes in carbon burial, palaeogeography and paleoceanography, which were linked to the initial breakup of Pangaea. In order to better understand the climate and environmental impact of this phase of major tectonic rearrangement we revisited a key section along the Dorset coast (UK) and used a multi-proxy geochemical approach for its analysis. Carbon isotopes (δ13C) measured on organic matter were corrected for the influence of changes in the type of organic matter. The thus obtained δ13C-HI index and an additional δ13Ccarb record measured on bulk carbonate indicate a suite of carbon-isotope excursions (CIEs) including the negative main CIE (Triassic-Jurassic boundary to early Sinemurian) the negative Sinemurian-Pliensbachian boundary CIE and the positive margaritatus Zone (late Pliensbachian) CIE. Based on elevated hydrogen index values and enrichments in Mo, U, V, total organic carbon (TOC) and pyrite, the Hettangian-Sinemurian interval was interpreted to have been characterised by high productivity conditions and strong oxygen depletion of the basin. The chemical alteration index (CIA), clay mineralogy, and δ18O data provide independent evidence for increased continental runoff triggered by humid climate conditions on the adjacent continent during this time period. The strongest oxygen-depleted conditions were inferred for the lowermost Hettangian interval, coincident with the onset of the main negative CIE. A further less strongly oxygen-depleted interval was recorded through the negative CIE near the Sinemurian-Pliensbachian boundary (jamesoni and ibex Zones). This interval was characterised by drier conditions and possibly cooler water temperatures (e.g. CIA, δ18Ocarb and kaolinite/illite ratio). Based on low CIA values and kaolinite/illite ratios, the Pliensbachian climate is considered to have been generally drier, superimposed by more humid episodes restricted to the davoei and margaritatus Zones. The long-term trend to drier conditions is explained by: (1) diminished atmospheric CO2 contents due to a reduction in emissions of volcanogenic greenhouse gases, and/or; (2) changes in the palaeogeography and current patterns related to the break-up of Pangaea and more specifically to the opening of the Hispanic corridor.

Great Oxidation and Lomagundi events linked by deep cycling and enhanced degassing of carbon.

For approximately the first 2 billion years of Earth history, atmospheric oxygen levels were extremely low. It wasn’t until at least half a billion years after the evolution of oxygenic photosynthesis, perhaps as early as 3 billion years ago, that oxygen rose to appreciable levels during the Great Oxidation event. Shortly after, marine carbonates experienced a large positive spike in carbon isotope ratios known as the Lomagundi event. The mechanisms responsible for the Great Oxidation and Lomagundi events remain debated. Using a carbon-oxygen box model which tracks surface and interior C fluxes and reservoirs while also tracking C isotopes and atmospheric oxygen levels we demonstrate that about 2.5 billion years ago a tectonic transition resulting in increased volcanic CO2 emissions could have led to increased deposition of both carbonates and organic carbon via enhanced weathering and nutrient delivery to oceans. Increased burial of carbonates and organic carbon would have allowed accumulation of atmospheric oxygen while also increasing delivery of carbon to subduction zones. Coupled with preferential release of carbonates at arc volcanoes and deep recycling of organic C to ocean island volcanoes we find such a tectonic transition can simultaneously explain the Great Oxidation and Lomagundi events without any change in the fraction of carbon buried as organic carbon relative to carbonate, which is often invoked to explain carbon isotope excursions.

Unravelling the sources of carbon emissions at the onset of Oceanic Anoxic Event (OAE) 1a

The early Aptian Oceanic Anoxic Event (OAE) 1a represents a major perturbation of the Earth's climate system and in particular the carbon cycle, as evidenced by widespread preservation of organic matter in marine settings and a characteristic negative carbon isotopic excursion (CIE) at its onset, followed by a broad positive CIE. The contemporaneous emplacement of a large igneous province (LIP) is invoked as a trigger for OAE 1a (and OAEs in general), but this link and the ultimate source of the carbon perturbation at the onset of OAEs is still debated. In this study, we simultaneously assimilate an atmospheric pCO2 reconstruction along with a δ13C record from the Spanish Cau section in an Earth system model to obtain a novel transient reconstruction of emission rates and identify the primary carbon-emitting sources across the negative CIE interval at the onset of OAE 1a. We reconstruct carbon emissions of 4,300–29,200 Pg from a mixture of carbon sources. This estimate is a lower bound, as contemporaneous organic carbon burial is not accounted for. Carbon was first released at slow rates from a 13C-depleted reservoir (e.g. thermo- and/or biogenic methane from sill intrusions). Towards the end of the negative CIE the rate of emissions increased and they became more 13C-enriched, likely from a dominantly volcanic source (e.g. LIPs). New osmium isotope (187Os/188Os) measurements, from the same section as the pCO2 reconstruction and δ13C data, reveal a shift to less radiogenic values coinciding with the change towards mantle-derived carbon emissions as indicated by our modelling results, lending further support to our interpretation. These results highlight that geologically triggered carbon emissions were likely driving the OAE onset.

Phosphorus burial in ferruginous SiO2-rich Mesoproterozoic sediments

Abstract Persistently low atmospheric oxygen requires that net organic carbon burial was muted through much of Earth’s middle age. In order to achieve global mass balance with respect to O2, recent models have suggested that redox-dependent mechanisms, such as Fe(II)-phosphate precipitation, limited phosphate availability in dominantly anoxic and ferruginous oceans, in turn limiting net primary production, and therefore organic carbon burial. Nevertheless, observational constraints on phosphorus cycling in ferruginous Proterozoic systems are rare, leaving these models largely untested. Here, we present high-resolution petrographic and mineralogical data showing that the 1.3 Ga Sherwin Ironstone (Roper Group, Australia) was dominated by syndepositional precipitation of the Fe(II)-silicate minerals greenalite and berthierine, interlaminated with abundant authigenic calcium fluorapatite (CFA). Set in a quantitative geochemical framework, these data reveal that elevated marine SiO2(aq) concentrations facilitated extensive Fe(II)-silicate production, leaving CFA, rather than Fe(II)-phosphate, as the principal inorganic phosphorous sink in shallow-water Roper Group sediments. More broadly, the physical and chemical factors that triggered Fe(II)-silicate and CFA burial in the Roper Seaway highlight semi-restricted basins as important loci of phosphorus removal from the mid-Proterozoic ocean.

A diurnal carbon engine explains 13C-enriched carbonates without increasing the global production of oxygen

In the past 3 billion years, significant volumes of carbonate with high carbon-isotopic ([Formula: see text]C) values accumulated on shallow continental shelves. These deposits frequently are interpreted as records of elevated global organic carbon burial. However, through the stoichiometry of primary production, organic carbon burial releases a proportional amount of [Formula: see text], predicting unrealistic rises in atmospheric [Formula: see text] during the 1 to 100 million year-long positive [Formula: see text]C excursions that punctuate the geological record. This carbon-oxygen paradox assumes that the [Formula: see text]C of shallow water carbonates reflects the [Formula: see text]C of global seawater-dissolved inorganic carbon (DIC). However, the [Formula: see text]C of modern shallow-water carbonate sediment is higher than expected for calcite or aragonite precipitating from seawater. We explain elevated [Formula: see text]C in shallow carbonates with a diurnal carbon cycle engine, where daily transfer of carbon between organic and inorganic reservoirs forces coupled changes in carbonate saturation ([Formula: see text]) and [Formula: see text]C of DIC. This engine maintains a carbon-cycle hysteresis that is most amplified in shallow, sluggishly mixed waters with high rates of photosynthesis, and provides a simple mechanism for the observed [Formula: see text]C-decoupling between global seawater DIC and shallow carbonate, without burying organic matter or generating O2.

Nitrogen and carbon cycle perturbations through the Cenomanian-Turonian oceanic anoxic event 2 (~94 Ma) in the Vocontian Basin (SE France)

Oceanic Anoxic Event 2, spanning the Cenomanian-Turonian boundary, was an episode of major perturbations in global biogeochemical cycles, characterized by the widespread burial of organic carbon in both oceans and epicontinental basins. In the Vocontian Basin (SE France), this event was expressed by the deposition of the Niveau Thomel, a succession of interbedded dark shales, grey marls and bioturbated carbonates as observed in the Lambruisse section. Analyses of the total organic carbon (TOC) content, combined with the concentrations of redox-sensitive trace elements, revealed that conditions fluctuated from oxic to suboxic-anoxic during the deposition of the Niveau Thomel. Transient periods of deoxygenation were related to perturbations in nutrient cycling reflected by near-zero to negative δ15N signatures indicative of increased diazotrophy.The development of deoxygenated conditions in the Vocontian Basin was likely preconditioned by hydrographically restricted conditions, as confirmed by Mo vs. U enrichments, reducing bottom-water ventilation associated with water-column stratification. Intervals of reoxygenation were likely linked to climate-induced changes. In particular, a phase of regional or global cooling associated with a drop in pCO2, called the Plenus Cold Event (PCE) resulted in vigorous reoxygenation of the Vocontian Basin.

Organic-carbon-rich sediments: benthic foraminifera as bio-indicators of depositional environments

Abstract. Fjords have been described as hotspots for carbon burial, potentially playing a key role within the carbon cycle as climate regulators over multiple timescales. Nevertheless, little is known about the long-term fate of the carbon that may become stored in fjordic sediments. One of the main reasons for this knowledge gap is that carbon arriving on the seafloor is prone to post-depositional degradation, posing a great challenge when trying to discriminate between an actual change in the carbon deposition rate and post-depositional carbon loss. In this study, we evaluate the use of modern benthic foraminifera as bio-indicators of organic carbon content in six voes (fjords) on the west coast of Shetland. Benthic foraminifera are known to be sensitive to changes in organic carbon content in the sediments, and changes in their assemblage composition therefore reflect synchronous variations in the quantity and quality of carbon reaching the seafloor. We identified four environments based on the relationship between benthic foraminiferal assemblages and organic carbon content in the sediments: (1) land-locked regions influenced by riverine and/or freshwater inputs of organic matter, namely the head of fjords with a restricted geomorphology; (2) stressed environments with a heavily stratified water column and sediments rich in organic matter of low nutritional value; (3) depositional environments with moderate organic content and mild or episodic current activity; and (4) marginal to coastal settings with low organic content, such as fjords with an unrestricted geomorphology. We conclude that foraminifera potentially provide a tool to disentangle primary organic carbon signals from post-depositional degradation and loss of organic carbon because of their environmental sensitivity and high preservation potential in the sedimentary record.

Land plant evolution decreased, rather than increased, weathering rates

Abstract The repeated evolution of trees is widely thought to have enhanced the capacity of silicate weathering via the impact of deep rooting. However, land plants are also responsible for wetland assembly and organic carbon burial. The total burial output of carbon via both organic and inorganic deposition must balance input to the exogenic system from volcanic outgassing on million-year time scales. Increased partitioning of carbon burial toward organic carbon and away from inorganic carbon reduces the marine carbonate burial flux, necessitating a lowered total flux of alkalinity to the oceans to maintain mass balance in the Earth’s surface carbon cycle. This flux includes the nutrient delivery from the terrestrial vegetation implicated as a driver of marine evolution, extinction, and environmental change including anoxia and black shale formation. Here, the burial of terrestrial organic carbon, first substantially in the Devonian and continuing through to the present, is argued to require a reduction in silicate weathering rates when compared to earlier times, given the independence of volcanic outgassing from weathering on short time scales. Land plants still may cause reductions in steady-state atmospheric CO2 levels, but via increasing the silicate weathering feedback strength, not silicate weathering rates. The mass-balance constraints on the long-term carbon cycle provide a mechanism for linking how land plant evolution simultaneously increased nutrient recycling and weathering efficiency of the Earth’s surface.

Middle Permian organic carbon isotope stratigraphy and the origin of the Kamura Event

Large carbon cycle perturbations associated with the Middle Permian (Capitanian) mass extinction have been widely reported, but their causes and timing are still in dispute. Low resolution carbon isotope records prior to this event also limit the construction of a Middle Permian chemostratigraphic framework and global or local stratigraphic correlation, and hence limit our understanding of carbon cycle and environmental changes. To investigate these issues, we analyzed the 13Corg values from the Middle Permian chert-mudstone sequence (Gufeng Formation) in the Lower Yangtze deep-water basin (South China) and compared them with published records to build a chemostratigraphic scheme and discuss the underlying environmental events. The records show increased δ13Corg values from late Kungurian to early Guadalupian, followed by a decrease to the late Wordian/early Capitanian. The early-mid Capitanian was characterized by elevated δ13Corg values suggesting the presence of the “Kamura Event”: an interval of heavy positive values seen in the δ13Ccarb record. We propose that these heavy Capitanian δ13C values may be a response to a marked decline in chemical weathering rates on Pangea and associated reduction in carbonate burial, which we show using a biogeochemical model. The subsequent negative δ13C excursion seen in some carbonate records, especially in shallower-water sections (and in a muted expression in organic carbon) coincide with the Capitanian mass extinction may be caused by the input of isotopically-light carbon sourced from the terrestrial decomposition of organic matter.

The impact of Early Cretaceous gateway evolution on ocean circulation and organic carbon burial in the emerging South Atlantic and Southern Ocean basins

Organic carbon burial is an important driver of carbon cycle and climate dynamics on geological and shorter time scales. Ocean basins emerging during the Early Cretaceous break-up of Gondwana were primary sites of organic carbon burial, implying that their tectonic and oceanographic evolution may have affected trends and perturbations in global climate via changes in local organic carbon burial. Assessing the role of individual ocean basins in the global carbon-climate context requires a sound understanding of the processes that induced large-scale changes in carbon burial and the timing of these changes. Here we reconstruct the oceanographic evolution, and its links to organic carbon burial, in the Barremian to Albian South Atlantic and Southern Ocean basins, which may have acted as carbon sinks of global importance. Our reconstruction is based on combined seawater neodymium isotope and sedimentological records obtained from multiple deep sea drill sites and a new general circulation model. Deep water circulation within and between those basins was primarily controlled by the opening of the shallow Falkland Plateau Gateway (between ∼118 Ma and ∼113 Ma) and the deep Georgia Basin Gateway (by ∼110 Ma), for which we provide new age constraints based on biostratigraphic and carbon isotope data. The opening of these gateways was accompanied by local to basin-wide decreases in organic carbon burial, suggesting that ocean circulation affected the oxygenation state via changes in deep water ventilation. Although our data do not provide quantitative information on the impact of changes in regional organic carbon burial on the global carbon cycle, the synchronicity between the reduction of organic carbon burial in the South Atlantic basin and global warming during the Early Albian points to a strong causal relationship.

Sustained wood burial in the Bengal Fan over the last 19 My

The Ganges-Brahmaputra (G-B) River system transports over a billion tons of sediment every year from the Himalayan Mountains to the Bay of Bengal and has built the world's largest active sedimentary deposit, the Bengal Fan. High sedimentation rates drive exceptional organic matter preservation that represents a long-term sink for atmospheric CO2 While much attention has been paid to organic-rich fine sediments, coarse sediments have generally been overlooked as a locus of organic carbon (OC) burial. However, International Ocean Discovery Program Expedition 354 recently discovered abundant woody debris (millimeter- to centimeter-sized fragments) preserved within the coarse sediment layers of turbidite beds recovered from 6 marine drill sites along a transect across the Bengal Fan (∼8°N, ∼3,700-m water depth) with recovery spanning 19 My. Analysis of bulk wood and lignin finds mostly lowland origins of wood delivered episodically. In the last 5 My, export included C4 plants, implying that coarse woody, lowland export continued after C4 grassland expansion, albeit in reduced amounts. Substantial export of coarse woody debris in the last 1 My included one wood-rich deposit (∼0.05 Ma) that encompassed coniferous wood transported from the headwaters. In coarse layers, we found on average 0.16 weight % OC, which is half the typical biospheric OC content of sediments exported by the modern G-B Rivers. Wood burial estimates are hampered by poor drilling recovery of sands. However, high-magnitude, low-frequency wood export events are shown to be a key mechanism for C burial in turbidites.

Authigenic metastable iron sulfide minerals preserve microbial organic carbon in anoxic environments

The burial of organic carbon (OC) in sedimentary environments promotes long-term carbon sequestration, which allows the release of oxygen in the atmosphere. Organo-mineral interactions that form between terrigenous minerals and OC during transport to and deposition on the seabed enhance OC preservation. Here, we propose an authigenic mechanism for the coupled preservation of labile OC and metastable iron sulfide minerals under anoxic conditions. Sulfate-reducing microorganisms (SRM) are ubiquitous in anoxic environments and produce the majority of free sulfide in marine sediments, leading to the formation of iron sulfide minerals in situ. Using high spatial resolution microscopy, spectroscopy and spectro-microscopy, we show that iron sulfide biominerals precipitated in the presence of SRM incorporate and adsorb organic molecules, leading to the formation of stable organo-mineral aggregates that could persist for years in anoxic environments. OC/iron sulfide assemblages consist of the metastable iron sulfide mineral phases mackinawite and/or greigite, along with labile organic compounds derived from microbial biomass or from organic molecules released extracellularly by SRM. Together these results underscore the role that a major group of anoxic microbes play in OC preservation and illustrate the value of the resulting authigenic metastable iron sulfide minerals mackinawite and greigite in protecting labile organic molecules from degradation over time.

Non-native mangroves support carbon storage, sediment carbon burial, and accretion of coastal ecosystems.

Mangrove forests play an important role in climate change adaptation and mitigation by maintaining coastline elevations relative to sea level rise, protecting coastal infrastructure from storm damage, and storing substantial quantities of carbon (C) in live and detrital pools. Determining the efficacy of mangroves in achieving climate goals can be complicated by difficulty in quantifying C inputs (i.e., differentiating newer inputs from younger trees from older residual C pools), and mitigation assessments rarely consider potential offsets to CO2 storage by methane (CH4 ) production in mangrove sediments. The establishment of non-native Rhizophora mangle along Hawaiian coastlines over the last century offers an opportunity to examine the role mangroves play in climate mitigation and adaptation both globally and locally as novel ecosystems. We quantified total ecosystem C storage, sedimentation, accretion, sediment organic C burial and CH4 emissions from ~70year old R. mangle stands and adjacent uninvaded mudflats. Ecosystem C stocks of mangrove stands exceeded mudflats by 434±33MgC/ha, and mangrove establishment increased average coastal accretion by 460%. Sediment organic C burial increased 10-fold (to 4.5MgCha-1 year-1 ), double the global mean for old growth mangrove forests, suggesting that C accumulation from younger trees may occur faster than previously thought, with implications for mangrove restoration. Simulations indicate that increased CH4 emissions from sediments offset ecosystem CO2 storage by only 2%-4%, equivalent to 30-60MgCO2 -eq/ha over mangrove lifetime (100year sustained global warming potential). Results highlight the importance of mangroves as novel systems that can rapidly accumulate C, have a net positive atmospheric greenhouse gas removal effect, and support shoreline accretion rates that outpace current sea level rise. Sequestration potential of novel mangrove forests should be taken into account when considering their removal or management, especially in the context of climate mitigation goals.

Ocean acidification and hypoxia alter organic carbon fluxes in marine soft sediments.

Anthropogenic stressors can alter the structure and functioning of infaunal communities, which are key drivers of the carbon cycle in marine soft sediments. Nonetheless, the compounded effects of anthropogenic stressors on carbon fluxes in soft benthic systems remain largely unknown. Here, we investigated the cumulative effects of ocean acidification (OA) and hypoxia on the organic carbon fate in marine sediments, through a mesocosm experiment. Isotopically labelled macroalgal detritus (13 C) was used as a tracer to assess carbon incorporation in faunal tissue and in sediments under different experimental conditions. In addition, labelled macroalgae (13 C), previously exposed to elevated CO2 , were also used to assess the organic carbon uptake by fauna and sediments, when both sources and consumers were exposed to elevated CO2 . At elevated CO2 , infauna increased the uptake of carbon, likely as compensatory response to the higher energetic costs faced under adverse environmental conditions. By contrast, there was no increase in carbon uptake by fauna exposed to both stressors in combination, indicating that even a short-term hypoxic event may weaken the ability of marine invertebrates to withstand elevated CO2 conditions. In addition, both hypoxia and elevated CO2 increased organic carbon burial in the sediment, potentially affecting sediment biogeochemical processes. Since hypoxia and OA are predicted to increase in the face of climate change, our results suggest that local reduction of hypoxic events may mitigate the impacts of global climate change on marine soft-sediment systems.

Kinetics and Identities of Extracellular Peptidases in Subsurface Sediments of the White Oak River Estuary, North Carolina.

Anoxic subsurface sediments contain communities of heterotrophic microorganisms that metabolize organic carbon at extraordinarily low rates. In order to assess the mechanisms by which subsurface microorganisms access detrital sedimentary organic matter, we measured kinetics of a range of extracellular peptidases in anoxic sediments of the White Oak River Estuary, NC. Nine distinct peptidase substrates were enzymatically hydrolyzed at all depths. Potential peptidase activities (Vmax) decreased with increasing sediment depth, although Vmax expressed on a per-cell basis was approximately the same at all depths. Half-saturation constants (Km ) decreased with depth, indicating peptidases that functioned more efficiently at low substrate concentrations. Potential activities of extracellular peptidases acting on molecules that are enriched in degraded organic matter (d-phenylalanine and l-ornithine) increased relative to enzymes that act on l-phenylalanine, further suggesting microbial community adaptation to access degraded organic matter. Nineteen classes of predicted, exported peptidases were identified in genomic data from the same site, of which genes for class C25 (gingipain-like) peptidases represented more than 40% at each depth. Methionine aminopeptidases, zinc carboxypeptidases, and class S24-like peptidases, which are involved in single-stranded-DNA repair, were also abundant. These results suggest a subsurface heterotrophic microbial community that primarily accesses low-quality detrital organic matter via a diverse suite of well-adapted extracellular enzymes.IMPORTANCE Burial of organic carbon in marine and estuarine sediments represents a long-term sink for atmospheric carbon dioxide. Globally, ∼40% of organic carbon burial occurs in anoxic estuaries and deltaic systems. However, the ultimate controls on the amount of organic matter that is buried in sediments, versus oxidized into CO2, are poorly constrained. In this study, we used a combination of enzyme assays and metagenomic analysis to identify how subsurface microbial communities catalyze the first step of proteinaceous organic carbon degradation. Our results show that microbial communities in deeper sediments are adapted to access molecules characteristic of degraded organic matter, suggesting that those heterotrophs are adapted to life in the subsurface.

Cenozoic carbonate burial along continental margins

Abstract Marine carbonate burial represents the largest long-term carbon sink at Earth’s surface, occurring in both deep-sea (pelagic) environments and shallower waters along continental margins. The distribution of carbonate accumulation has varied over geological history and impacts the carbon cycle and ocean chemistry, but it remains difficult to quantitatively constrain. Here, we reconstruct Cenozoic carbonate burial along continental margins using a mass balance for global carbonate alkalinity, which integrates independent estimates for continental weathering and pelagic carbonate burial. Our results indicate that major changes in marginal carbonate burial were associated with important climate and sea-level change events, including the Eocene-Oligocene transition (ca. 34 Ma), the Oligocene-Miocene boundary Mi-1 glaciation (ca. 23 Ma), and the middle Miocene climate transition (ca. 14 Ma). In addition, we find that a major increase in continental weathering from ca. 10 Ma to the present may have driven a concomitant increase in pelagic carbonate burial. Together, our results show that changes in global climate, sea level, and continental weathering have all impacted carbonate burial over the Cenozoic, but the relative importance of these processes may have varied through time.