Carbon Tetrachloride-induced Oxidative Stress Research Articles

Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing.

Acute liver injury (ALI) has the potential to compromise hepatic function rapidly, with severe cases posing a considerable threat to human health and wellbeing. Conventional treatments, such as the oral administration of antioxidants, can inadvertently lead to liver toxicity and other unwanted side effects. Mesenchymal stromal cells (MSCs) can target therapeutic agents directly to inflammatory sites owing to their homing effect, and they offer a promising avenue for the treatment of ALI. However, the efficacy and feasibility of these live cell products are hampered by challenges associated with delivery pathways and safety concerns. Therefore, in this work, MSC membranes were ingeniously harnessed as protective shells to encapsulate synthesized PLGA nanoparticle cores (PLGA/MSCs). This strategic approach enabled nanoparticles to simulate endogenous substances and yielded a core-shell nano-biomimetic structure. The biomimetic nanocarrier remarkably maintained the homing ability of MSCs to inflammatory sites. In this study, cryptotanshinone (CPT)-loaded PLGA/MSCs (CPT@PLGA/MSC) were prepared. These nanoparticles can be effectively internalized by LO2 cells. They reduced cellular oxidative stress and elevated inflammatory levels. In vivo results suggested that, after intravenous administration, CPT@PLGA/MSCs significantly reduced uptake by the reticuloendothelial system and immune recognition compared to PLGA nanoparticles without MSC membrane coatings, subsequently resulting in their targeted and enhanced accumulation in the liver. The effectiveness of CPT@PLGA/MSCs in alleviating carbon tetrachloride-induced oxidative stress and inflammation in a mouse model was unequivocally demonstrated through comprehensive histological examination and liver function tests. This study introduces a pioneering strategy with substantial potential for ALI treatment.

Effects of Oral Administration of Pulegone on Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

Effects of Oral Administration of Pulegone on Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

Investigation of the phytochemical composition and remedial effects of southern grape hyacinth (Muscari neglectum Guss. ex Ten.) plant extract against carbon tetrachloride-induced oxidative stress in rats

We aimed to determine the phytochemical contents of the aerial part M. neglectum aerial part (MAP) and M. neglectum bulb (MB) ethanolic extract of Muscari neglectum and to investigate their protective effects on gastric damage induced by carbon tetrachloride (CCl4) in rats. After the toxicity testing, 42 female Wistar albino rats were divided into 7 groups, Control, MAP, MB, CCl4, CCl4 + MAP, CCl4 + MB, and CCl4 + Silymarin groups. At the end of the experiment, the serum biochemical parameters, antioxidant defense enzymes, and malondialdehyde (MDA) contents in the stomach tissue were evaluated to determine the antioxidant role of the M. neglectum extracts. According to the gas chromatography–mass spectroscopy, fatty acid analysis, octadecadienoic, and 9,12,15 octadecatrienoic fatty acids were found as major fatty acids in the MAP, whereas 9,12 octadecadienoic and octadecanoic acids were the major fatty acids in the MB. According to the liquid chromatography–tandem mass spectrometry, quinic acid, fumaric acid, gentisic acid, caffeic acid, kaempferol, and apigenin were found in the MAP, while quinic acid, fumaric acid, caffeic acid, and kaempferol were found in the MB. The total phenolic and flavonoid contents in the extract were determined in the MAP and MB. The MAP and MB extracts generally caused a statistically significant decrease in the MDA content and increase in the antioxidant parameters in the stomach tissue. It was concluded that MAP and MB extracts may have antioxidant and gastric protective effects due to the phytochemical content of M. neglectum. Highlights According to LC-MS/MS results, quinic acid, fumaric acid, chemferol, apigenin, and caffeic acid were determined as major compounds in M. neglectum extracts. According to GC-MS results, octadecadienoic, octadecatrienoic, and octadecanoic methyl esters were the major fatty acids of the M. neglectum extracts. The M. neglectum extracts regulated the levels of stomach damage and biochemical parameters. The M. neglectum extracts extract might have pharmaceutical-nutritional potential.

Pregabalin, a GABA Analogue Protects against Carbon Tetrachloride-Induced Oxidative Stress and Liver Injury in Rats

Pregabalin is a synthetic analogue of the neurotransmitter g-aminobutyric acid (GABA) and is used in the treatment of epilepsy and neuropathic pain. The aim of this study was to investigate the effect of pregabalin on toxic liver injury caused by the acute administration of carbon tetrachloride (CCl4). Rats were orally treated with CCl4 for two successive days either alone or along with intraperitoneal pregabalin at doses of 7 and 14 mg/kg. The control group received the vehicle (olive oil). Liver oxidative stress and damage were assessed by determining serum and/or liver tissue levels of malondialdehyde (MDA), nitric oxide (NO), reduced glutathione (GSH), DNA fragmentation, serum aminotransferases, paraoxonase-1 (PON-1), and hepatic histopathology. Results showed that CCl4 significantly (i) increased MDA and NO and decreased PON-1 in both serum and liver tissue, and (ii) decreased liver GSH content and induced marked hepatic DNA fragmentation. CCl4 treatment caused liver tissue injury as evidenced by significantly increased serum aminotransferases. In line with the above biochemical changes, the liver of CCl4-treated rats exhibited massive steatosis, vacuolar degeneration, cloudy swelling, and necrosis. In CCl4-treated rats, pregabalin given at the dose of 14 mg/kg significantly reduced the increments in MDA, NO, serum aminotransferases, and hepatic DNA fragmentation. Liver GSH was unaltered but hepatic and serum PON-1 activity increased after administering pregabalin which also improved, though not normalized, liver tissue histopathology. Collectively, these results suggest that the administration of pregabalin is associated with a reduction in experimental liver injury caused by CCl4. (First online: Apr 12, 2021)

Protective role of protocatechuic acid in carbon tetrachloride-induced oxidative stress via modulation of proinflammatory cytokines levels in brain and liver of Wistar rats.

Protocatechuic acid (PCA) possesses numerous pharmacological activities, including antioxidative and anti-inflammatory activities. This study seeks to investigate its underlying mechanism of action in the liver and brain toxicity induced by CCl4 in male albino rats. Rats were given PCA at 10 and 20mg/kg daily and orally as a pretreatment for seven days. A single injection of CCl4 was given 2h later to induce brain and liver toxicity. CCl4 moderately elevated the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). PCA lowered AST level significantly when compared to control. Total protein and albumin levels presented insignificant changes (p>0.05) in all groups while lipid profile showed increased total cholesterol level and reduced high-density lipoprotein (HDL) by CCl4. PCA (10mg/kg) significantly reduced the cholesterol level while the 20mg/kg dose moderately prevented HDL reduction. There was an increased MDA production with a corresponding low GSH level in the group treated with CCl4. Activities of superoxide dismutase, catalase, and glutathione-S-transferase in both organs also declined. PCA, especially at 10mg/kg attenuated lipid peroxidation by increasing GSH level in the organs. Biochemical assays revealed the improvement of antioxidant enzyme activities by PCA in these organs. Furthermore, PCA lowered the level of proinflammatory cytokine COX 2 in the brain and liver while NF-kB expression was inhibited in the brain. Histopathology reports validated the effects of PCA. PCA exhibited protection against toxicity in these tissues through antioxidant and anti-inflammatory activities and the potential mechanism might be through modulation of the NF-κB/COX-2 pathway.

Experimental Evaluation of Prophylactic and Therapeutic Antioxidant Potential of Trimetazidine in Carbon Tetrachloride Induced Oxidative Stress in Rats

Introduction: Exposure to various drugs and chemicals lead to oxidative stress. Carbon Tetrachloride (CCl4) produces rise in oxidative stress leading to hepatic damage. The drug Trimetazidine (TMZ) shows hepatoprotective activity but its mechanism is not known. The present study would help in establishing antioxidant activity of TMZ as probable mechanism. Aim: To evaluate the antioxidant potential of TMZ in CCl4 induced oxidative stress when given prophylactically/therapeutically in rats. Materials and Methods: An experimental animal study was conducted on 80 adult Wistar rats of either sex (weight-150 to 200 gm) from March 2010 to December 2010 in Bharati Vidyapeeth Medical College, Pune, Maharashtra, India. Randomly, all animals were grouped into 10 equal groups. Group i was normal control (received only water). To induce oxidative stress CCl4 (0.5 mL/kg/d i.p.) was given to all the animals of Group ii to Group x for seven days. The TMZ was given in two doses, TMZ1 (5 mg/kg orally for Group iii and vii) and TMZ2 (10 mg/kg orally for Group iv and viii). Positive standard control (Group v and Group ix) received Liv.52 (1 mL/kg orally). Group vi and Group x received combination of TMZ1 (5 mg/kg orally)+Liv.52 (1 mL/kg orally). Drug treatment was given to animals in group iii, iv, v and vi for 1-14 days (preventive group) and in group vii, viii, ix and x from day 8 to day 14 (therapeutic group). On 15th day, rats were sacrificed and dissected for collection of liver. Part of the livers was homogenised to assess oxidative stress marker enzymes Malondialdehyde (MDA), Superoxide Dismutase (SOD) spectrophotometrically. Statistical analysis was done with one- way Analysis of Variance (ANOVA) followed by post-hoc analysis (Dunnett’s test) using GraphPad Prism 5.0 software. Results: Trimetazidine (5 mg/kg and 10 mg/kg) significantly reduced MDA levels and increased SOD levels when compared with CCl4 treated group suggested antioxidant activity. Combined administration of Liv.52 and TMZ1 also reduced oxidative stress and increased antioxidant activity. Conclusion: Results of the present study suggested that increased oxidative stress was significantly attenuated by drug TMZ in dose dependant manner when compared with the CCl4 group. The antioxidant potential of prophylactic and therapeutic administration of TMZ was comparable. The increased antioxidant effect by Liv.52+TMZ1 combination was only due to the additive antioxidant effects of Liv.52 and TMZ or any other mechanism was involved, needs to be further evaluated.

Investigation of Antioxidant Compositions and Antioxidative Activities of Ethanol Extract of <I>Alstonia boonei </I>Stem Bark

Background: Oxidative stress is the major mechanism of action employed by many drugs, xenobiotics and toxicants that generate reactive metabolites in the body to elicit adverse reactions when there are insufficient antioxidants to neutralise the excess reactive metabolites.
 Objectives: This study evaluated antioxidant compositions and antioxidative activities of ethanol extract of Alstonia boonei stem bark with the view of understanding its ameliorative potentials on oxidative stress.
 Materials and Methods: Thirty-six rats divided into 6 groups of 6 rats each was used for this study. Group 1 served as normal control, group 2-6 were carbon tetrachloride-induced oxidative stress which served as treatment groups. Group 2 was untreated, group 3 was treated with silymarin (100 mg/kg) while group 4-6 were treated with the extract 100, 200 and 500 mg/kg body-weight for 28 days respectively. The animal study and antioxidant compositions of the extract were analysed using standard methods.
 Results: The extract was found to rich in antioxidant components such as phenols, flavonoids, β-carotene and lycopene. The extract showed high DPPH (2, 2-diphenyl-1-picrylhydrazyl) and nitric oxide radical scavenging activities and high ferric reducing antioxidant power with increasing concentrations. The significant (P < 0.05) reduction in antioxidant enzymes activities and increased level of lipid peroxidation caused by the carbon tetrachloride induction were significantly (P < 0.05) reversed, in the extract-treated groups relative to the normal control.
 Conclusions: These findings suggest that the extract possesses antioxidative properties and can be used in the management of oxidative stress and its related disease conditions.
 Keywords: Antioxidant components, Alstonia boonei stem bark, Antioxidative stress, Lipid peroxidation, Antioxidant enzymes.

Fabrication, characterization and biological evaluation of silymarin nanoparticles against carbon tetrachloride-induced oxidative stress and genotoxicity in rats.

This study aimed to synthesize silymarin nanoparticles (SILNPs) using chitosan nanoparticles as a delivery system and to evaluate their protective effects against CCl4 in rats. Eight groups of male Sprague-Dawley rats were treated for three weeks included the control group, CCl4-treated group (100mg/kg b.w twice a week); SIL-treated group (50mg/lg b.w); the groups treated daily with low dose (LD) or high dose (HD) of SILNPs (25, 50mg/kg b.w) and the groups treated with CCl4 plus SIL, SILNPs (LD) or SILNPs (HD). Blood and tissue samples were collected for different assays. The synthesized SILNPs showed a smooth rounded shape with average particle size of 100±2.8nm. SILNPs contain the same compounds found in raw SIL and the in vitro release of SILNPs continues till 24h. The in vivo study revealed that SIL and SILNPs at the low or high dose induced a significant improvement in the hematological parameters, liver and kidney function, lipid profile, serum cytokines, gene expression DNA fragmentation and histology of liver and kidney tissue resulted from CCl4. It could be concluded that SILNPs can be applied in oral delivery formulations with a potential application value for liver disease therapy.

β-Sitosterol attenuates carbon tetrachloride-induced oxidative stress and chronic liver injury in rats.

Chronic liver diseases are clinically silent and responsible for significant morbidity and mortality worldwide. β-Sitosterol (BSS), major phytosterol in plants, has a wide spectrum of protective effect against various chronic ailments. We investigated the hepatoprotective effect of BSS against carbon tetrachloride (CCl4)-induced chronic liver injury in rats. Thirty rats were divided into five groups, with six animals in each group. Group I rats served as control while groups II, III, IV, and V rats were injected intraperitoneally with CCl4 (0.2mL/100g b.w. in olive oil (1:1)) for 7 consecutive weeks. After 7weeks, group II rats were left without any treatments and served as CCl4 alone group, while groups III, IV, and V rats were treated with BSS 25 and 50mg/kg b.w. and silymarin 100mg/kg b.w. as oral post-treatments respectively, for the next 4weeks. At the end of the experiment, hepatotoxicity marker enzymes in serum, oxidative stress, and fibrosis marker were analyzed. CCl4 administration caused significant elevation of marker enzymes of hepatotoxicity in serum and increased lipid peroxidation and fibrosis markers such as hydroxyproline, collagen, α-smooth muscle actin, vimentin, desmin, and matrix metalloproteinases 9 in liver tissue of rats. This treatment also caused a significant diminution of intracellular enyzmicantioxidants such as SOD and CAT in the liver tissue of rats. All the above adversities were significantly mitigated by the BSS post-treatments. The results suggest that BSS could have a hepatoprotective effect against oxidative stress-mediated CLD induced by CCl4.

HMG-CoA reductase inhibitor, rosuvastatin averted carbon tetrachloride-induced oxidative stress, inflammation and fibrosis in the liver of rats

HMG-CoA reductase inhibitor, rosuvastatin averted carbon tetrachloride-induced oxidative stress, inflammation and fibrosis in the liver of rats

The protective effect of Liza klunzingeri protein hydrolysate on carbon tetrachloride-induced oxidative stress and toxicity in male rats.

Objective(s):Today, consumers are looking for food products providing health benefits in addition to meeting the basic nutritional needs of the body. This study aimed to evaluate the antioxidant and cytotoxic effects of Liza klunzingeri protein hydrolysate both in vivo and in vitro.Materials and Methods:Fish protein hydrolysate (FPH) was prepared using enzymatic hydrolysis with papain. In vitro antioxidant activity was assessed using five different antioxidant assays. The cytotoxic effect on 4T1 cell line was evaluated using the MTT assay. The distribution of the molecular weight of FPH was measured using HPLC. In the in vivo study, CCl4-exposed Wistar rats were orally treated with FPH (150, 300, and 600 mg/kg) or gallic acid (50 mg/kg) for 28 consecutive days.Results:Enzymatic hydrolysis gave hydrolysate rich in low molecular weight peptides (<1000 Da) with strong free radicals (ABTS, DPPH, and OH) scavenging activity and cytotoxicity. Treatment of CCl4-exposed rats with all doses of FPH significantly lowered serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). FPH at doses of 300 and 600 mg/kg significantly decreased lipid peroxidation and improved total antioxidant capacity in serum, liver, and kidney of the CCl4 exposed rats. All doses of L.klunzingeri protein hydrolysate reduced CCl4-induced nitric oxide production of the kidney. Liver histopathological damage caused by CCl4 also ameliorated with all doses of FPH.Conclusion: L. klunzingeri protein hydrolysate can be considered as a functional food to alleviate oxidative stress

The Effects of Oral Administration of Pulegone in Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

The Effects of Oral Administration of Pulegone in Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

Application of nanoencapsulated silymarin to improve its antioxidant and hepatoprotective activities against carbon tetrachloride-induced oxidative stress in broiler chickens

The objective of this study was to evaluate antioxidant and hepatoprotective activities of nanoencapsulated silymarin (NES) against carbon tetrachloride-induced oxidative stress in broiler chickens. An in vitro study was conducted using a 20 kHz ultrasound with 62.5% amplitude to produce NES, stabilized by alginate for controlled release of its bioactive compounds in simulated gastrointestinal tract of broiler chickens. Results showed that sonication reduced the size of particles from 657.5 to 169.1 nm and polydispersity index from 0.60 to 0.31.Simulated gastrointestinal digestion in vitro indicated that NES was more resistant to gastric fluid compared to intestinal fluid. An animal model study was designed to investigate the antioxidant and hepatoprotective effects of NES on broiler chickens subjected to carbon tetrachloride-mediated oxidative stress. A total of 640 one-day-old mail broiler chickens were randomly assigned to 8 treatments with 4 replicate cages of 20 broiler chickens per cage as 2 × 2 × 2 factorial arrangement of treatments in a completely randomized design, including 2 concentrations of silymarin (0 and 100 mg/kg of body weight), NES (0 and 100 mg/kg of body weight), and carbon tetrachloride (0 and 1 mL/kg of body weight). All broiler chickens were fed a common diet throughout the study. From d 21, broiler chickens of control group received saline (intraperitoneally), while other treatment groups received silymarin (orally), carbon tetrachloride (intraperitoneally), and NES (orally). On d 42, 2 broiler chickens per replicate cage were euthanized for blood and tissue sampling. The interactions of silymarin × carbon tetrachloride (P = 0.003) and NES × carbon tetrachloride (P = 0.001) improved protein metabolism and lipid oxidative stability (P = 0.001). Combination of NES and carbon tetrachloride decreased serum content of alanine aminotransferase (P = 0.001), whereas combination of silymarin and carbon tetrachloride and NES increased (P = 0.02) its serum content as compared to broiler chickens that received none of those treatments. Carbon tetrachloride down-regulated (P = 0.03) the expression of superoxide dismutase 1, while silymarin, NES, and combination of silymarin and NES up-regulated its expression (P = 0.03). In contrast to carbon tetrachloride (P = 0.001), NES down-regulated (P = 0.03) heat shock protein 47 as compared with broiler chickens that received none of those treatments. In conclusion, NES has potential to mitigate the oxidative stress-induced hepatotoxicity in broiler chickens through modulation of oxidative stress biomarkers and expression of superoxide dismutase 1 and heat shock protein 47 genes.

Hepatoprotective effects of ethyl pyruvate against carbon tetrachloride-induced oxidative stress, biochemical and histological alterations in rats

This study investigated the protective effects of ethyl pyruvate (EP) against carbon tetrachloride (CCl4)-induced acute hepatic injury in rats. The administration of a single dose of CCl4 (1.6 g/kg body weight) significantly elevated the levels of malondialdehyde, nitric oxide, alanine transaminase, aspartate transaminase, and alkaline phosphatase, cholesterol, low-density lipoprotein cholesterol, and triglycerides. In addition, CCl4 was found to significantly suppress the activity of superoxide dismutase, catalase, and glutathione peroxidase. All of these parameters were restored to their normal levels by the administration of EP before and after the CCl4 injection. Moreover, the number of positive apoptotic hepatocytes had significantly increased in the CCl4 group but decreased in rats treated with EP along with CCl4. Histopathological changes induced by CCl4 were also ameliorated by EP treatment. These findings provided evidence that EP, because of its antioxidant and anti-apoptotic action, could protect rat liver against CCl4-induced acute liver injury.

Ameliorative effect of Homalium zeylanicum against carbon tetrachloride-induced oxidative stress and liver injury in rats

Objective is to evaluate the ameliorative effects of Homalium zeylanicum in carbon tetrachloride-induced oxidative stress and liver injury in rats. To establish the nature of antioxidant principles in the bioactive ethyl acetate fractions of bark (HZEB) and leaf (HZEL); oxygen radical absorbance capacity (ORAC) and cell-based antioxidant protection in erythrocytes (CAP-e) assays were performed. From acute toxicity study, HZEB and HZEL at 200 and 300 mg/kg b.w., were relatively safe at their effective doses. The degree of protection was measured by using biochemical parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin (TB) and total protein (TP) contents. Hepatic markers e.g. thiobarbituric acid reactive species (TBARS), superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) were evaluated along with histopathological observations of liver tissues. Both fractions showed significant improvement in restoring SGOT, SGPT, ALP, TB and TP level. TBARS, SOD, CAT and GSH levels were significantly altered towards normal values. Both fractions at 300 mg/kg showed remarkable improvement in liver markers as compared to silymarin. Histopathological examinations showed reduction in hepatic necrosis and appeared normal hepatocellular architecture in HZEB and HZEL treated groups. In CAP-e assay, IC50 of HZEB (54.66 mg/mL) was higher than HZEL (60.88 mg/mL) and in ORAC assay, AUC of HZEB and HZEL were 33.46, 21.29 respectively and results were comparable with trolox. GC–MS and LC–MS analysis identified a total no. of 44 compounds. Few compounds were identified as bioactive compounds e.g. catechol (7.23%), tetraacetyl-d-xylonic nitrile (3%), oleic acid (0.49%), 2,6-bis(1,1-dimethylethyl)-phenol (3.71%), 3,4,5-trimethoxy-phenol (0.31%), and conifer alcohol (7.41%). The presence of antioxidant principles in both fractions were responsible for hepatoprotective activities, however, the presence of catechol (7.23%) in the bark part imparted better activities in protecting liver than leaf of H. zeylanicum.

Syzygium aromaticum (clove) effect on catalase activity due to carbon tetrachloride-induced oxidative stress in rat liver

Background: Clove is known as antioxidant spice that used in cigarettes, spice for food or soup, and traditional medicine. It is believed that cloves could protect smokers from cigarette-free radicals. Otherwise, the study on clove as an antioxidant was still confused.&#x0D; Objective: To reveal that clove can overcome carbon tetrachloride (CCl4) and its free-radical derives&#x0D; Method: This study was experimental research, using 20 Wistar rats that were divided into 4 groups, Group 1 (CCl4 + cloves 3), group 2 (CCl4 + cloves 1), group 3 (normal control, without being offered treatment), group 4 (positive control, induced by CCl4 and followed by 100 mg alpha-tocopherol), and group 5 (negative control, only induced by CCl4). Rat livers were homogenized and followed with CAT activity measurement using the spectrophotometry method of Mates.&#x0D; Results: There was a significant difference in mean between the groups (p= 0,001). Further test, the Post Hoc showed that there is a significance different between group 1 and 4 (p=0.008), 1 and 5 (p=0.001), 2 and 5 (p=0.001), 3 and 5 (p=0.001), and 4 and 5 (p=0.007). Group 1 (CCl4+Clove3) has the highest catalase activity.&#x0D; Conclusion: Syzygium aromaticum (clove) oral administration with the dose of 200 mg/kg rat body weight against 0.55 mg/kg BW CCl4 show an increase of catalase activity but did not overcome the oxidative stress.

Investigation of the protective effects of horse mushroom (Agaricus arvensis Schaeff.) against carbon tetrachloride-induced oxidative stress in rats.

Wild and cultured mushrooms have been extensively used for food and medicinal purposes all around the world. However, there is limited information on chemical composition, health enhancing effects and contributions on diet of some mushrooms (e.g., Agaricus arvensis) widely distributed in many countries including United Kingdom, Australia, Turkey etc. Therefore, the present study was aimed to analyse the bioactive composition and ameliorative effects of A. arvensis via evaluating in vitro and in vivo antioxidant properties in CCl4 induced rat model. The extract exhibited higher antioxidant capacities in vitro than that of the positive control (Reishi-Shiitake-Maitake standardized extract). Administration of the extract had significant regulative effects in the levels of AST, ALT, LDH, Urea and TRIG levels according to CCl4 group. Additionally, lipid peroxidation and GSH in the brain, kidney and liver tissues was regulated by extract treated groups compared to the CCI4 group. The supplementation of the extract at the dose of 100mg/kg regulated the levels of GST, GR, CAT and GPx enzyme activities in brain and liver, but not in kidney tissue. There was approximately three fold increase in CAT enzyme activity in kidney tissue of extract treated groups compared to Control and CCl4 groups. The extract contained a rich composition of bioactive compounds including phenolics (protocatechuic acid and p-hydroxybenzoic acid), volatile compounds (benzaldehyde, palmitic acid and linoleic acid) and mineral compounds (K, Si, Mg and Na). Data obtained within this study suggests that A. arvensis might be used for food industries in order to obtain nutritional products.

Protective effect of rutin against carbon tetrachloride-induced oxidative stress, inflammation and apoptosis in mouse kidney associated with the ceramide, MAPKs, p53 and calpain activities

Rutin, a natural flavonoid, possess beneficial health effects. However, its renoprotective effect against carbon tetrachloride (CCl4) induced injury and the underlying mechanism is not clarified. The current study aims is to identify the therapeutic effects of rutin on oxidative stress, inflammation and apoptosis in mouse kidney exposed to CCl4. ICR mice received CCl4 with or without rutin co-administration for one week. Compared with the control group, mice receiving CCl4 alone showed kidney injury as evidenced by elevation in serum biochemical markers, inflammation, caspase-3 activity and apoptosis in kidney, while rutin administration significantly attenuated these pathophysiological changes. Exploration of the underlying mechanisms of its action demonstrated that rutin reduced the ROS, calpain and ceramide levels in mouse kidneys. Rutin significantly decreased the p53, TNF-α, IL-1β activities and mitogen-activated protein kinase (MAPK) phosphorylation in the kidneys. In addition, rutin increased the levels of Bcl-2 protein and reduced levels protein of Bax. Rutin also inhibited the release of cytochrome C from mitochondria in kidneys of the CCl4-treated mice. Taken together, rutin ameliorates CCl4-induced oxidative stress, inflammation and apoptosis through regulating the ceramide, MAPK, p53 and calpain activities and thereby suppressing apoptosis by the mitochondrial pathway.

Protective effect of juice extract of mesocarp rind of Citrullus lanatus in carbon tetrachloride-induced oxidative stress in rats

Protective effect of juice extract of mesocarp rind of Citrullus lanatus in carbon tetrachloride-induced oxidative stress in rats

Ameliorative and antidyslipidemic potentials of aqueous leaf extract of Gongronema latifolium in CCl4 carbon tetrachloride-induced oxidative stress rats

Ameliorative and antidyslipidemic potentials of aqueous leaf extract of Gongronema latifolium in CCl4 carbon tetrachloride-induced oxidative stress rats