China’s forests act as a large carbon sink and play a vital role in achieving the carbon neutrality goal by the 2060s. To achieve this goal, the magnitude and spatial patterns of forest carbon sinks must be accurately quantified. In this study, we aim to provide the longest estimate of forest biomass carbon storage and sinks in China at a 1 km spatial resolution from 1990 to 2021 by merging long-term observations from optical and microwave remote sensing datasets with a field-validated benchmark map. We explored the spatial characteristics of aboveground biomass (AGB) and belowground biomass (BGB) carbon in China’s forests, as well as variations in AGB carbon sinks. The average AGB and BGB carbon storage from 1990 to 2021 in China’s forests were 8.42 ± 0.96 Pg C and 1.9 ± 0.21 Pg C, respectively. The average annual AGB carbon sink during this period was approximately 0.083 ± 0.023 Pg C yr−1. Forests in the southwest region contributed 31.15% of the forest AGB carbon sink in China and contributed 41.01% of the forest AGB carbon storage. Our study presents an effective tool for assessing changes in forest biomass carbon by leveraging comprehensive multi-source remote sensing data and highlights the importance of obtaining large-scale, high-quality, consistent, and accessible plot survey data to validate the earth observation of biomass.