Soil Organic Carbon Research Articles

Nutrient Addition Enhances the Temperature Sensitivity of Soil Carbon Decomposition Across Forest Ecosystems.

Atmospheric nitrogen (N) and phosphorus (P) depositions have been shown to alter nutrient availability in terrestrial ecosystems and thus largely influence soil carbon cycling processes. However, the general pattern of nutrient-induced changes in the temperature response of soil carbon decomposition is unknown. Yet, understanding this pattern is crucial in terms of its effect on soil carbon-climate feedback. Here, we report that N and P additions significantly increase the temperature sensitivity of soil organic carbon decomposition (Q10) by sampling soils from 36 sites across China's forests. We found that N, P, and their co-addition (NP) significantly increased the Q10 by 11.3%, 11.5%, and 23.9%, respectively. The enhancement effect of nutrient addition on Q10 was more evident in soils from warm regions than in those from cold regions. Moreover, we found that nutrient-induced changes in substrate availability and initial substrate and nutrient availability mainly regulated nutrient addition effects. Our findings highlight that N and P deposition enhances the temperature response of soil carbon decomposition, suggesting that N and P deposition should be incorporated into Earth system models to improve the projections of soil carbon feedback to climate change.

Multi-temporal assessment of a wildfire chronosequence by remote sensing

The study aimed to develop a methodological framework to identify forest ecosystems affected by wildfires and evaluate their recovery chronologically. To do this remote sensing analysis, sites with burn scars were selected based on various criteria (fire severity, affected area, vegetation and soil type, slope, aspect, and one-time occurrence of wildfire in the last 23 years). Spectral vegetation indices (VIs) from satellite imagery were used to estimate burn severity and vegetation cover changes. Images of surface reflectance were obtained from the collection of Landsat 5 ETM, Landsat 7 ETM+, and Landsat 8 OLI/TIRS, available and processed on the Google Earth Engine Platform (GEE). Indices VIs (i) the normalized difference vegetation index (NDVI), (ii) the normalized burn ratio (NBR), and (iii) the differenced normalized burn ratio (dNBR) were calculated to classify burn severity. The one-time occurrence selection was performed using the LandTrendr algorithm to monitor changes in land cover and burned areas. To validate the selection, the chosen sites within the chronosequence were clustered on 4 seasons of soil properties and litter accumulation recovery. Our result can guide methodological comparisons and forest management practices on large surfaces by comparing parches of different time-affected ecosystems. Validation sites of the cluster chronosequence shows consistent recovery of soil properties as soil carbon, bulk density and litter accumulation through the studied years•The study developed a framework to identify wildfire-affected forest ecosystems and evaluate their recovery using remote sensing and local data.•Vegetation indices (NDVI, NBR, dNBR) from Landsat satellite imagery processed on the Google Earth Engine were used to assess burn severity and vegetation changes over time.•Selected sites were validated using the LandTrendr algorithm and monitored for seasonal changes in soil properties and litter accumulation.

Soil carbon and nitrogen cycling at the atmosphere-soil interface: Quantifying the responses of biocrust-soil interactions to global change.

In drylands, where water scarcity limits vascular plant growth, much of the primary production occurs at the soil surface. This is where complex macro- and microbial communities, in an intricate bond with soil particles, form biological soil crusts (biocrusts). Despite their critical role in regulating C and N cycling in dryland ecosystems, there is limited understanding of the fate of biologically fixed C and N from biocrusts into the mineral soil, or how climate change will affect C and N fluxes between the atmosphere, biocrusts, and subsurface soils. To address these gaps, we subjected biocrust-soil systems to experimental warming and drought under controlled laboratory conditions, monitored CO2 fluxes, and applied dual isotopic labeling pulses (13CO2 and 15N2). This allowed detailed quantification of elemental pathways into specific organic matter (OM) pools and microbial biomass via density fractionation and phospholipid fatty acid analyses. While biocrusts modulated CO2 fluxes regardless of the temperature regime, drought severely limited their photosynthetic C uptake to the extent that the systems no longer sustained net C uptake. Furthermore, the effect of biocrusts extended into the underlying 1 cm of mineral soil, where C and N accumulated as mineral-associated OM (MAOM<63μm). This was strongly associated with increased relative dominance of fungi, suggesting that fungal hyphae facilitate the downward C and N translocation and subsequent MAOM formation. Most strikingly, however, these pathways were disrupted in systems exposed to warming, where no effects of biocrusts on the elemental composition of the underlying soil nor on MAOM were determined. This was further associated with reduced net biological N fixation under combined warming and drought, highlighting how changing climatic conditions diminish some of the most fundamental ecosystem functions of biocrusts, with detrimental repercussions for C and N cycling and the persistence of soil organic matter pools in dryland ecosystems.

Rice straw biochar and NPK minerals for sustainable crop production in arid soils: a case study on maize-wheat cropping system

Maize and wheat are the main cereals grown in Egypt. However, the country relies on grain imports to meet its local demands. In order to improve their production, appropriate fertilization programs are needed. The present study investigates the effects of amending a clayey soil of an arid region with rice straw biochar and NPK mineral fertilizers, individually or in combination, for increasing growth and productivity of maize and wheat crops. Additionally, impacts of these additives on soil biological activities and carbon (C) transformations in soil were a matter of concern herein. To achieve this objective, a field research of a randomized block design was conducted during the summer (maize) and winter (wheat) seasons of 2020/2021. The following treatments were considered: unmodified control (CK), 100% N inputs in the form of biochar (reference organic treatment, RSB) (T1), 100% mineral treatment (reference inorganic treatment, T2), 75% RSB + 25% NPK minerals (T3), 50% RSB + 50% NPK minerals (T4) and 25% RSB + 75% NPK minerals (T5). Additional doses of mineral fertilizers were added to treatments from T3 to T5 to maintain NPK inputs within the recommended doses. Key results showed that all additives significantly enhanced plant growth parameters and productivity. They also increased soil organic carbon level by the end of the growing season hence reduced soil bulk density, even for the treatment that received only mineral NPK applications (T2). All additives also upraised soil cation exchange capacity (CEC), soil available nitrogen (N), and soil salinity. However, sole application of biochar recorded the least increase in soil salinity. Combined mineral-organic treatments not only recorded the highest increases in soluble and microbial fractions of organic carbon and nitrogen in soil; but also noted the greatest improvements in growth and grain productivity of maize and wheat versus sole applications of mineral fertilizers or biochar. The alkaline nature of biochar was buffered by soil while no significant differences were observed in harvest index among treatments. In conclusion, combined use of biochar and mineral fertilizers, especially T5 is recommended for increasing soil fertility and wheat and maize grain productvity.

The application of organic mulch and chicken manure for improving soil water availability and yield of turmeric (Curcuma domestica Val) in an Inceptisol of Jambi, Indonesia

Turmeric (Curcuma domestica Val) is one of the agricultural commodities being developed by the Jambi Province of Indonesia. However, despite the high value of turmeric, its productivity in Jambi Province is low. This is because the crop is mostly cultivated in marginal lands dominated by Inceptisol, which has low fertility and low water availability. Organic mulch and chicken manure have the potential to be used to improve soil water availability and crop yield. This research aimed to evaluate the effect of organic mulch cover and chicken manure on soil water availability and turmeric yield. The treatments tested were combinations of various percentages of organic mulch cover (30%, 60%, and 90%, and chicken manure dosage (0, 5, 10, and 15 t ha-1). The twelve treatment combinations were arranged in a randomized block design with three replications. The results showed that the combination of the percentage of organic mulch cover and chicken manure dosage affected soil organic matter content, soil bulk density, soil pore size distribution, soil water availability, and turmeric yield. The application of 30% cover of organic mulch and 10 t ha-1 of chicken manure was found to be the best combination to improve soil available water and turmeric yield. The regression analysis results showed that soil bulk density, organic carbon, fast-drainage pores, and slow-drainage pores simultaneously affected the soil water content, with an R2 value of 0.85. The results of this study proved that soil available water is also closely correlated with turmeric yield.

Water holding capacity, aggregation, respiration, and chemical character of acid soil amended rice straw biochar enriched with different volumes of liquid extract (sap) of Kappapychus alvarezii

The quality of acidic soil is determined by organic C content produced from rice straw biochar in agriculture. In this context, liquid extract from Kappapychus alvarezii (K-sap) is used as a biochar enrichment agent. Therefore, this research aimed to (i) analyze the character of K-sap enriched rice straw biochar with different volumes, as well as (ii) evaluate the impact on soil water holding capacity, size class distribution, aggregate stability index, respiration rate, and acidic soil chemical characters. The treatment tested was the volume of K-sap kg-1 biochar, namely (i) without biochar, (ii) 0 mL, (iii) 500 mL, (iv) 1,000 mL, and (v) 1,500 mL. Each treatment was repeated three times and placed according to a randomized block design procedure. The area covered by K-sap, pore size, and amorphous degree increased while the pore volume of the biochar surface decreased. The addition of 1,000 mL of K-sap kg-1 biochar released a new peak number associated with the aliphatic and aromatic groups. The K-sap enriched biochar increased the proportion of soil aggregate size of 1-2 mm, water holding capacity, carbon storage, pH, total N, available P and K, exchangeable base cations as well as base saturation. Meanwhile, the concentration of Al3+ and H+ were decreased in the acidic soil solution. The results showed that the performance of rice straw biochar, K-sap volumes, soil chemical quality, water holding capacity, and ability to store carbon of the acidic soil was improved by adding K-sap volume.

Belowground diversity drives multifunctionality in grazing pastures on the eastern Tibetan Plateau

Livestock grazing can alter ecosystem structure, functions, and services across diverse biomes, with grazing intensity being a key factor affecting grassland function. Although the effects of grazing on plant and soil properties have been extensively studied, the effects of grazing intensity on biodiversity and ecosystem multifunctionality (EMF) remain largely unexplored. Therefore, this study addresses this gap using 28 indicator variables from a well-controlled yak grazing intensity experiment in alpine meadows on the eastern Tibetan Plateau. The results showed that aboveground diversity (calculated using plant species richness and insect diversity) exhibited a hump-shaped and significant response to increasing grazing intensity, multidiversity (whole-ecosystem biodiversity) and belowground diversity (calculated using nematode richness and microbial diversity) showed no significant response, and EMF significantly declined. Grazing decreased carbon and nitrogen cycling indices (calculated by carbon and nitrogen in plants and soils), but did not affect phosphorus cycling. Structural equation modelling indicated that EMF was directly affected by grazing intensity and belowground diversity (i.e., nematode and fungal diversity), rather than by multidiversity, aboveground diversity, and plant pathogens. Grazing-induced decreases in plant pathogens showed no direct or indirect effects on EMF but increased multidiversity and aboveground diversity. Overall, our results highlight the critical role of conserving belowground diversity in promoting and maintaining multifunctionality in grazing pastures on the Tibetan Plateau.

Permafrost Region Greenhouse Gas Budgets Suggest a Weak CO2 Sink and CH4 and N2O Sources, But Magnitudes Differ Between Top‐Down and Bottom‐Up Methods

AbstractLarge stocks of soil carbon (C) and nitrogen (N) in northern permafrost soils are vulnerable to remobilization under climate change. However, there are large uncertainties in present‐day greenhouse gas (GHG) budgets. We compare bottom‐up (data‐driven upscaling and process‐based models) and top‐down (atmospheric inversion models) budgets of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) as well as lateral fluxes of C and N across the region over 2000–2020. Bottom‐up approaches estimate higher land‐to‐atmosphere fluxes for all GHGs. Both bottom‐up and top‐down approaches show a sink of CO2 in natural ecosystems (bottom‐up: −29 (−709, 455), top‐down: −587 (−862, −312) Tg CO2‐C yr−1) and sources of CH4 (bottom‐up: 38 (22, 53), top‐down: 15 (11, 18) Tg CH4‐C yr−1) and N2O (bottom‐up: 0.7 (0.1, 1.3), top‐down: 0.09 (−0.19, 0.37) Tg N2O‐N yr−1). The combined global warming potential of all three gases (GWP‐100) cannot be distinguished from neutral. Over shorter timescales (GWP‐20), the region is a net GHG source because CH4 dominates the total forcing. The net CO2 sink in Boreal forests and wetlands is largely offset by fires and inland water CO2 emissions as well as CH4 emissions from wetlands and inland waters, with a smaller contribution from N2O emissions. Priorities for future research include the representation of inland waters in process‐based models and the compilation of process‐model ensembles for CH4 and N2O. Discrepancies between bottom‐up and top‐down methods call for analyses of how prior flux ensembles impact inversion budgets, more and well‐distributed in situ GHG measurements and improved resolution in upscaling techniques.

Intercropping Legumes Improves Long Term Productivity and Soil Carbon and Nitrogen Stocks in Sub‐Saharan Africa

AbstractFood, feed, and fiber production needs to increase to support demands of the growing population in Sub‐Saharan Africa (SSA), while soil fertility continues to decline. Intercropping, the cultivation of two or more crop species on the same field, can provide yield benefits and is suggested to positively affect soil organic carbon (C) and nitrogen (N) stocks. This study uses the biogeochemical model system LandscapeDNDC with the objective to (a) represent maize‐legume intercropping systems in different bioregions in SSA by simultaneously simulating both crops and their interactions and (b) assess long‐term (20 years) impacts of intercropping under varying mineral fertilizer inputs (0–150 kg N ha−1 yr−1) on productivity as well as soil organic C and N stocks. We test LandscapeDNDC on 82 field data sets (site‐year‐treatment combinations) from 18 sites to represent yields and soil C/N dynamics of maize‐legume intercropping systems. Using the model for long‐term scenario simulations showed that intercropping allows to sustain productivity and to improve or maintain SOC stock in low or zero fertilizer systems if all residues are returned to the soil. In contrast, for sole‐cropped maize systems, a decline in SOC stocks was simulated unless a minimum of 35 kg N ha−1 yr−1 of fertilizer was applied at full residue return. We conclude that intercropping using legumes alongside sufficient residue return allows for stabilizing long‐term yields while avoiding SOC losses even with low fertilizer N inputs. Overall, our study confirms the potential of intercropping as a sustainable agricultural practice that could significantly contribute to food security in SSA.

Reduced ligninase‐cellulase ratio enhances soil carbon sequestration following afforestation of agricultural land

AbstractIntroductionAfforestation of agricultural land is one of the most essential approaches to mitigate climate change by enhancing the sequestration of atmospheric carbon (C) into the soil. C‐degrading extracellular enzymes produced by soil microbes regulated the decomposition and fate of sequestrated soil organic carbon (SOC), with potential divergent variations following afforestation across different ecosystem scales. However, the feedbacks of different C‐degrading enzymes and their relationships with SOC following afforestation of agricultural land remain unclear.Materials and MethodsWe investigated the changes in enzyme activity and their relationships with SOC in soil aggregates across two typical climatic vegetation restoration regions in China, and explored the mechanisms through which changes in enzyme activity contribute to SOC sequestration following afforestation of agricultural land.ResultsAfforestation of agricultural land generally decreased ligninase activity and increased cellulase activity across various aggregate fractions, compared to the adjacent croplands in both subtropic (Danjiangkou Reservoir, DJK) and temperate (Maoershan, MES) region. Additionally, the ratio of ligninase to cellulase (L:C) was lower in afforested lands than in the croplands, with L:C as the major factor explaining the variations of SOC sequestration following afforestation. Specifically, ligninase and L:C were negatively correlated with SOC, whereas cellulase showed positive correlations with SOC. Further analyses suggested that microbial biomass C and nitrogen (MBC and MBN) and the ratio of SOC and total nitrogen (SOC:TN) were important factors influencing L:C and subsequently regulating SOC. These results suggest that shifts in microbial enzyme production from ligninase to cellulase following afforestation, reduced the decomposition of recalcitrant C, thus contributing to SOC sequestration.ConclusionOur work underscores the critical role of reduced L:C in enhancing SOC sequestration following the restoration of croplands to afforested lands. These findings advance the understanding of the influence of microbial community physiological adaptations on C sequestration across different land use types.

Resolving the Intricate Effects of Multiple Global Change Drivers on Root Litter Decomposition.

Plant roots represent about a quarter of global plant biomass and constitute a primary source of soil organic carbon (C). Yet, considerable uncertainty persists regarding root litter decomposition and their responses to global change factors (GCFs). Much of this uncertainty stems from a limited understanding of the multifactorial effects of GCFs and it remains unclear how these effects are mediated by litter quality, soil conditions and microbial functionality. Using complementary field decomposition and laboratory incubation approaches, we assessed the relative controls of GCF-mediated changes in root litter traits and soil and microbial properties on fine-root decomposition under warming, nitrogen (N) enrichment, and precipitation alteration. We found that warming and N enrichment accelerated fine-root decomposition by over 10%, and their combination showed an additive effect, while precipitation reduction suppressed decomposition overall by 12%, with the suppressive effect being most significant under warming-alone and N enrichment-alone conditions. Significantly, changes in litter quality played a dominant role and accelerated fine-root decomposition by 15% ~ 18% under warming and N enrichment, while changes in soil and microbial properties were predominant and reduced decomposition by 7% ~ 10% under precipitation reduction and the combined warming and N enrichment. Examining only the decomposition environment or litter properties in isolation can distort global change effects on root decomposition, underestimating precipitation reduction impacts by 38% and overstating warming and N effects by up to 73%. These findings highlight that the net impact of GCFs on root litter decomposition hinges on the interplay between GCF-modulated root decomposability and decomposition environment, as well as on the synergistic or antagonistic relationships among GCFs themselves. Our study emphasizes that integrating the legacy effects of multiple GCFs on root traits, soil conditions and microbial functionality would improve our prediction of C and nutrient cycling under interactive global change scenarios.

Effects of temperature, relative humidity and soil organic carbon content on soil-air partitioning coefficients of volatile PFAS.

Soil-air partitioning coefficient (KSA) values are often used to assess the environmental fate of organic contaminants in soil. Till now, sufficient KSA values have not yet been measured for many compounds of interest, including some emerging pollutants such as volatile PFAS. Moreover, the effects of environmental factors such as temperature, relative humidity and soil organic carbon content on KSA of volatile PFAS are also unclear. In this study, the KSA values of target volatile PFAS were measured under various temperature (20-40 °C), relative humidity (30-100 %) and soil organic carbon content (2.1 %-8.0 %) using a modified solid-phase fugacity meter. The results showed that higher temperatures, higher relative humidity and lower organic carbon content in soil may accelerate the diffusion of target volatile PFAS. Furthermore, the KSA measurements were used to derive a multiple linear regression model to depict the relationship between logKSA and temperature, relative humidity, soil organic carbon content and PFAS-specific logKOA. When compared with the predictions obtained from semi-empirical model, we argued that the multiple linear regression model is more robust and easier to implement for target volatile PFAS or other emerging volatile PFAS than the semi-empirical approach to help depict the diffusion process at target volatile PFAS contaminated sites.

Evaluation of nature-based climate solutions for agricultural landscapes in the Galápagos Islands

The Galápagos Islands are highly vulnerable to climate and environmental change, and nature-based solutions can help local communities adapt local agricultural systems. Through a comparative analysis, we evaluated the effects of three land management strategies on soil temperature, soil water availability and storage, and carbon stocks in Santa Cruz Island (Galápagos Archipelago). We installed six monitoring sites that consisted of two replicates per pathway: (i) the avoided loss of tropical forest, (ii) the conservation of scattered trees and living fences in at-risk agroforestry system, and (iii) the increase in biomass after a reduction of the grazing intensity. The monitoring sites were equipped with a dense network of rain gauges, air temperature and relative humidity sensors, and capacitance/frequency probes that registered volumetric water content and soil temperature. After pedological characterization of the soil profiles, the soil physico-chemical and hydrophysical properties were determined in laboratory. Over a period of 30 months (July 2019 to December 2021), hydrometeorological and soil environmental data were collected.We assessed differences in soil temperature, moisture availability and soil organic carbon content between native forests, sites under traditional agroforestry and under passive restoration. Forest soils were 12 % cooler;, and soil moisture under forest was 20 % higher than in parcels with silvopastural management. Forest soils had a lower dry bulk density, lower saturated hydraulic conductivity and higher water retention capacity in comparison with the other two management types. In silvopastural systems, a decrease of grazing intensity had a positive effect on soil carbon stocks, that were about 50 % higher than in soils under traditional management. This study shows that avoided loss of tropical forest within an agricultural landscape is a promising strategy to mitigate increasing soil temperatures, agricultural drought, and decline in soil organic carbon content. Continued monitoring of the experimental sites is necessary to corroborate the findings of this investigation at longer temporal scales.

Revealing suitable habitats for Juniperus procera and Olea europaea tree species in the remnant dry Afromontane forests of Ethiopia: Insights from ensemble species distribution modeling approach.

Human activities and climate change pose a significant threat to the dry Afromontane forests in Ethiopia, which are essential for millions of people both economically and ecologically. In Ethiopia, trees are planted elsewhere even if they are not likely to be well suited to the area. This study aims to identify the suitable habitat for the most exploited Juniperus procera (J. procera) and Olea europaea (O. europaea) tree species in northern Ethiopia. As inputs, least correlated temperature, moisture, soil, and topographic variables were selected through a stepwise procedure. The study evaluated five individual and ensemble models using the area under the curve (AUC) and true skill statistic (TSS) values. The ensemble model outperformed with mean AUC of 0.95 and TSS of 0.78 for J. procera, while securing the second position for O. europaea with an AUC of 0.88 and TSS of 0.71. Climatic factors emerged as the most influential, followed by soil and topography. Suitable areas for both species were found when Isothermality (Bio3) values range from 52% to 62%, temperature seasonality (Bio4) of 16-29°C. Moreover, well drained soils with soil texture not heavier than sandy clay, and soil organic carbon ranging from 5 to 42 g kg-1 were found suitable. The optimal suitable altitude for J. procera and O. europaea was determined to be 2200-2600 and 2100-2500 m.a.s.l., respectively. The suitable areas for J. procera and O. europaea were estimated to be 3130 and 3946 km2, respectively. Furthermore, potential plantation areas were identified beyond Desa'a and Hugumbirda Grat-Kahsu protected forests, covering 2721 km2 (86.9%) for J. procera and 3576 km2 (90.6%) for O. europaea. These findings hold significance for the conservation and sustainable management of these valuable tree species in northern Ethiopia. We recommend implementing a similar approach for other locally restricted dry Afromontane tree species with wider potential distribution.

Vertical migration flux of soil carbon and nitrogen of sloping farmland in &amp;lt;?A3B2 pi6?&amp;gt;typical black soil areas

Vertical migration flux of soil carbon and nitrogen of sloping farmland in &amp;lt;?A3B2 pi6?&amp;gt;typical black soil areas

Changes in Soil Bacterial and Fungal Community Composition and Functional Groups During the Artificial Restoration of Degraded Grassland of "Black-Soil Mountain".

About 35% of grassland in Sanjiangyuan area of China has degenerated into black-soil mountain. Artificial grassland is considered to be an effective measure to alleviate the severely degraded grassland in the alpine region of the three rivers and has been widely used. However, the pattern, potential function, and changes of carbon and nitrogen contents of soil microorganisms in degraded grassland in Heimushan by planting artificial grassland are still unclear. In this study, mixed-sown artificial alpine grassland (AG) was the focus of our study, whereas degraded black-soil mountain grassland (BG) and natural alpine grassland (NG) served as controls. Illumina 16S and ITS gene sequence analyses were used to analyze the community structure of the soil bacteria and fungi. The functional groups of NG, AG, and BG were predicted using the FAPROTAX and FUNGuild databases. In addition, the levels of soil carbon, nitrogen, and soil enzyme activities were evaluated. The results indicated a significant increase in the aboveground biomass of BG due to the planting artificial grassland. Moreover, the contents of total carbon (TC), total nitrogen (TN), ammonium nitrogen ( ), microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), and leucine aminopeptidase (LAP) increased in the soil. Planting artificial grasslands changed the composition of bacterial and fungal communities. Among these, the bacterial community was more sensitive to planting artificial grasslands. The relative abundance of bacterial functional groups involved in carbon and nitrogen cycling changed significantly, suggesting that bacteria may play a role in regulating nutrient cycling during artificial grassland planting. Soil TC, TN, LAP, and affected the microbial community structure related to carbon and nitrogen. and β-1,4-glucosidase were carbon and nitrogen factors, respectively, that affected functional changes in fungi. These results indicate that planting artificial grasslands can effectively enhance the productivity of degraded black-soil mountain and regulate soil microbial communities and soil physical and chemical properties.

Exploring compost production potential and its economic benefits and greenhouse gas mitigation in Addis Ababa, Ethiopia

The increasing amount of municipal organic waste (MOW) and human excreta (HE) has led to socio-economic and environmental challenges in the cities of developing countries. This study estimated MOW and HE, compost production potential from MOW and HE, and compost application potential for urban agriculture fertilization, economic benefits, soil carbon sequestration, and greenhouse gas (GHG) mitigation in Addis Ababa, Ethiopia, for the period 2025–2050. MOW was forecasted using the Holt-Winters forecasting model. HE was estimated using the daily average rate of HE generation. The compost production potential was estimated using the forecasted MOW and HE. Compost fertilization was determined by considering compost nitrogen (N), phosphorus (P), and potassium (K) and the fertilizer requirements of cereals and vegetables. The economic benefits of compost were determined by considering the price of compost-equivalent urea, NPS, and potassium chloride fertilizers. The mitigation of GHG emissions from compost application was estimated using the IPCC Tier 1 method. The forecasted quantities of MOW, HE, and compost for 2050 are 301, 462, and 343 Gg, respectively. The compost could supply 5 Gg of N and 2.2 Gg of P in 2050, sufficient to fertilize 14,129 ha of vegetable fields. The economic benefits of using compost as a substitute for synthetic fertilizers could reach 10 million USD in 2050. Compost production and application could offset the total GHG emissions of Addis Ababa by 13.1 % (10,241Gg CO2-eq year−1) in 2050. The application of compost generated from MOW and HE in Addis Ababa can substitute synthetic fertilizers, provide economic benefits, and mitigate GHG emissions.

Elevated concentrations of soil carbon dioxide with partial root-zone drying enhance drought tolerance and agro-physiological characteristics by regulating the expression of genes related to aquaporin and stress response in cucumber plants

Water scarcity and soil carbon dioxide elevation in arid regions are considered the most serious factors affecting crop growth and productivity. This study aimed to investigate the impacts of elevated CO2 levels (eCO2 at rates of 700 and 1000 ppm) on agro-physiological attributes to induce drought tolerance in cucumbers by activating the expression of genes related to aquaporin and stress response, which improved the yield of cucumber under two levels of irrigation water conditions [75% and 100% crop evapotranspiration (ETc)]. Therefore, two field experiments were conducted in a greenhouse with controlled internal climate conditions, at the Mohamed Naguib sector of the national company for protected agriculture, during the winter seasons of 2021–2022 and 2022–2023. The treatments included eCO2 in soil under normal and partial root zoon drying (PRD, 100% ETc Full irrigations, and 75% ETc). All the applied treatments were organized as a randomized complete block design (RCBD) and each treatment was replicated six times. Untreated plants were designed as control treatment (CO2 concentration was 400 ppm). The results of this study showed that elevating CO2 at 700 and 1000 ppm in soil significantly increased plant growth parameters, photosynthesis measurements, and phytohormones [indole acetic acid (IAA) and gibberellic acid (GA3)], under partial root-zone drying (75% ETc) and full irrigation conditions (100% ETc). Under PRD condition, eCO2 at 700 ppm significantly improved plant height (13.68%), number of shoots (19.88%), Leaf greenness index (SPAD value, 16.60%), root length (24.88%), fresh weight (64.77%) and dry weight (61.25%) of cucumber plant, when compared to untreated plants. The pervious treatment also increased photosynthesis rate, stomatal conductance, and intercellular CO2 concentration by 50.65%, 15.30% and 12.18%; respectively, compared to the control treatment. Similar findings were observed in nutrient concentration, carbohydrate content, Proline, total antioxidants in the leaf, and nutrients. In contrast, eCO2 at 700 ppm in the soil reduced the values of transpiration rate (6.33%) and Abscisic acid (ABA, 34.03%) content in cucumber leaves compared to untreated plants under both water levels. Furthermore, the results revealed that the gene transcript levels of the aquaporin-related genes (CsPIP1-2 and CsTIP4) significantly increased compared with a well-watered condition. The transcript levels of CsPIP improved the contribution rate of cell water transportation (intermediated by aquaporin’s genes) and root or leaf hydraulic conductivity. The quantitative real-time PCR expression results revealed the upregulation of CsAGO1 stress-response genes in plants exposed to 700 ppm CO2. In conclusion, elevating CO2 at 700 ppm in the soil might be a promising technique to enhance the growth and productivity of cucumber plants in addition to alleviating the adverse effects of drought stresses.

Direct Evidence for Microbial Regulation of the Temperature Sensitivity of Soil Carbon Decomposition.

Soil physicochemical protection, substrates, and microorganisms are thought to modulate the temperature sensitivity of soil carbon decomposition (Q10), but their regulatory roles have yet to be distinguished because of the confounding effects of concurrent changes of them. Here, we sought to differentiate these effects through microorganism reciprocal transplant and aggregate disruption experiments using soils collected from seven sites along a 5000-km latitudinal transect encompassing a wide range of climatic conditions and from a 4-year laboratory incubation experiment. We found direct microbial regulation of Q10, with a higher Q10 being associated with greater fungal:bacterial ratios. However, no significant direct effects of physicochemical protection and substrate were observed on the variation in Q10 along the latitudinal transect or among different incubation time points. These findings highlight that we should move forward from physicochemical protection and substrate to microbial mechanisms regulating soil carbon decomposition temperature sensitivity to understand and better predict soil carbon-climate feedback.

Enhanced carbon use efficiency and warming resistance of soil microorganisms under organic amendment

The frequency and intensity of extreme weather events, including rapid temperature fluctuations, are increasing because of climate change. Long-term fertilization practices have been observed to alter microbial physiology and community structure, thereby affecting soil carbon sequestration. However, the effects of warming on the carbon sequestration potential of soil microbes adapted to long-term fertilization remain poorly understood. In this study, we utilized 18O isotope labeling to assess microbial carbon use efficiency (CUE) and employed stable isotope probing (SIP) with 18O-H2O to identify growing taxa in response to temperature changes (5–35 °C). Organic amendment with manure or straw residue significantly increased microbial CUE by 86–181 % compared to unfertilized soils. The microorganisms inhabiting organic amended soils displayed greater resistance of microbial CUE to high temperatures (25–35 °C) compared to those inhabiting soils fertilized only with minerals. Microbial growth patterns determined by the classification of taxa into incorporators or non-incorporators based on 18O incorporation into DNA exhibited limited phylogenetic conservation in response to temperature changes. Microbial clusters were identified by grouping taxa with similar growth patterns across different temperatures. Organic amendments enriched microbial clusters associated with increased CUE, whereas clusters in unfertilized or mineral-only fertilized soils were linked to decreased CUE. Specifically, shifts in the composition of growing bacteria were correlated with enhanced microbial CUE, whereas modifications in the composition of growing fungi were associated with diminished CUE. Notably, the responses of microbial CUE to temperature fluctuations were primarily driven by changes in the bacterial composition. Overall, our findings demonstrate that organic amendments enhance soil microbial CUE and promote the enrichment of specific microbial clusters that are better equipped to cope with temperature changes. This study establishes a theoretical foundation for manipulating soil microbes to enhance carbon sequestration under global climate scenarios.