Carbohydrate Content Research Articles

Nutrients and Antioxidant Composition of Local Condiment “Ogiri” Produced from Different Leguminous Seeds

‘Ogiri’ refers to a fermented oily paste that is used as soup condiments for its strong smell. Apart from the fact that it’s mostly used in soup preparation because of the strong pungent odour and organoleptic properties, it also impact nutrients to soup. This study investigated the nutrients and antioxidant composition of Ogiri produced from different leguminous seeds. Raw material such as melon (EGU) and sesame (SES) seeds were procured from Oja Oba (Oba Market) in Owo, Ondo State while pumpkin (PUM) and Castor (CAS) seed were purchased at Upper Iweka market, Onitsha Anambra State, Nigeria. The raw materials were processed into ‘Ogiri’ using traditional methods and were subjected to chemical analysis using standard analytical methods.” Data generated were subjected to One-way Analysis of Variance (ANOVA) and means were separated using Duncan Multiple Range Test at 5% level of significance. Findings shows that the moisture content was significantly (P<0.05) higher in PUM than any other sample. Sample SES was significantly (P<0.05) higher in Ash (10.22g /100g) content, and fibre (11.21g/100g) while EGU was significantly (P<0.05) higher in fat (30.17g/100g) and protein (29.30g/100g). CAS had the highest carbohydrate content (27.58g/100g).Minerals result shows that Na (14.835mg/g), Ca (22.570mg/100g) and Mg (3.351mg/100g) were significantly (P<0.05) higher in sample EGU. Sample SES was significantly higher in K (55.855mg/100g) and Zn (0.308mg/100g), while CAS was significantly lower (P<0.05) in Na (3.45mg/100g), Ca (13.15mg/100g) K, (21.655mg/100g), Zn (0.128mg/100g) and Mg (1.925mg/mg) but has the highest value in P (12,252mg/100g). PUM was significantly higher in Vitamin E (8.270mg/100g), while CAS was significantly higher in vitamin A (7.576UI/g). Sample PUM had the highest Polyphenol and Terpenoid content (106.82mg/g and 1.37mg/g respectively) while sample SES had the highest steroid content (0.92mg/g). In conclusion, this study revealed that the nutrients and antioxidant properties of the “Ogiri” samples vary proportionately. The results can guide farmers, processors, and consumers in making informed decisions regarding seed selection and Ogiri preparation.

Effect of kefir grain concentration on rheological, microbiological, and physicochemical characteristics of bovine colostrum kefir

Bovine colostrum has high nutrition content such as protein, fat, peptide, micro-nutrients, antimicrobial components and bioactive compounds that benefit the body. Kefir is one way of diversifying food products as fermented beverage products to increase the utilization and functional properties of bovine colostrum, which has a unique taste. Production of colostrum kefir requires the right concentration of kefir grains to obtain high-quality characteristics. Evaluating the effect of kefir grain concentration on the rheological, microbiological, and physicochemical characteristics of bovine colostrum kefir was the purpose of this research. This study used one factorial design with the variation of kefir grain concentrations 10% (T1), 20% (T2), and 30% (T3) (w/v) with seven replications. Rheological analysis was conducted, followed by total lactic acid bacteria, yeast, microbes, protein, fat, ash, water content, carbohydrate, pH, total dissolved solids and alcohol content. Based on the result, different kefir grain concentrations significantly decreased total LAB, yeast, microbes, protein, ash, pH, and total dissolved solids but increased carbohydrate and water content with the addition of kefir grain concentration. Rheology behavior, fats, and alcohol content had no significant difference. Treatment of 10% of kefir grain concentration is the most suitable as it contains the highest total LAB and yeast.

Peatland organic matter quality varies with latitude as suggested by combination of FTIR and Ramped Pyrolysis Oxidation.

We employed two compelling and distinct methods, Fourier Transform Infrared Spectroscopy (FTIR) and Ramped Pyrolysis Oxidation (Ramped PyrOx), to examine the quality of organic matter (OM) stored in four peatlands located along a latitudinal gradient (Tropical (4˚N), Subtropical (27˚N), Boreal (48˚N), and Polar (68˚N)). FTIR was used to quantify the relative abundance of carbohydrates, a relatively labile compound class, and aromatics, which are more recalcitrant, in a sample set of four peat cores. These samples were then prepared using Ramped PyrOx, a second, independent method of determining OM quality that mimics the natural diagenetic maturation of OM that would take place over long timescales. Previous large-scale studies using FTIR to evaluate OM quality have observed that it generally increases with increasing latitude (more carbohydrates, less aromatics). Here, we demonstrate that the Ramped PyrOx approach both validates and complements the FTIR approach. The data stemming from each Ramped PyrOx preparation was input to a model that generates an estimated probability density function of the activation energy (E) required to break the C bonds in the sample. We separated these functions into three fractions ("low E," "medium E," and "high E") to create Ramped PyrOx variables that could be quantitatively compared to the compound class abundance data from FTIR. In assessing the agreement between the two methods, we found three significant relationships between Ramped PyrOx and FTIR variables. Low E fractions and carbohydrate content were positively correlated (R2 = 0.51) while low E fractions were negatively correlated with aromatic content (R2 = 0.58). Medium E fractions were found to be positively correlated with aromatics (R2 = 0.69).

Therapeutic potential of β-hydroxybutyrate in the management of pancreatic neoplasms: exploring novel diagnostic and treatment strategies.

Pancreatic neoplasm, a highly aggressive and often fatal cancer, poses challenges due to late detection and nonspecific symptoms. Therefore, both early diagnosis and appropriate therapeutic approaches are necessary to augment the condition of these patients. Cancer cells undergo metabolic deregulation, which enables their proliferation, survival, and invasion. As a result, it is crucial to focus on the metabolic pathways in prevalent cancers and explore treatment strategies that target these pathways to control tumor growth effectively. This is particularly relevant in cancers like pancreatic cancer, which undergo numerous metabolic alterations. The ketogenic regimen, characterized by low carbohydrate and protein contents and high-fat sources, does not involve caloric restriction. This allows for the induction of ketogenesis and an increase in ketone bodies, while insulin and glucose levels remain low even after meals. This unique metabolic state may influence the tumor microenvironment. Given the lack of unanimous agreement on the precise role and mechanism of the ketogenic diet, this review aims to clarify the diagnostic value and accuracy of ketone bodies in various types of pancreatic tumors and explore the potential anti-cancer effects of the ketogenic diet when used alone or in conjunction with chemotherapy, also to determine the potential of the ketogenic diet to be used as adjuvant therapy. The outcomes of this study are instrumental in enhancing our understanding of the benefits and drawbacks associated with employing this diet for the management and diagnosis of pancreatic cancer.

Identification and Analysis of the Nutrition Value of Black Teripang (Holothuriaedulis) and Sand Teripang (Holothuria Scabra) In the Waitiu Flow of Lewolema District

Indonesia is a country with a large area of ​​water, with enormous potential for marine biodiversity which is useful as food, pharmaceuticals and cosmetics. There are several marine biota that produce secondary metabolites that are beneficial to humans, including microorganisms, blue green algae, green algae, brown algae, red algae, sponges, coelenterates, bryozoans, molluscs and sea cucumbers (echinoderms). Sea cucumbers are marine animals classified in the phylum Echinodermata, class Holothuroidea. This animal lives on sandy substrates or can be found in coral ecosystems. Sea cucumbers have complete and quite high nutritional content, but the existence of sea cucumbers with a high population in East Flores Regency has not been utilized properly. This study aims to identify and analyze the nutritional content of Sand Sea Cucumber (Holothuria Scabra) and Black Sea Cucumber (Holothuria Edulis). Identification is based on morphology and body color and spicules of the dorsal integument tissue, while the analysis of nutritional value includes water content and ash content using the gravimetric method, protein content using the Kjeldahl method, fat content using the Soxhlet method and carbohydrate content using the by difference method. The results of the study showed that the nutritional content of Sand Sea Cucumber (Holothuria Scabra) was 5.472% water content, 51.056% ash content, 27.072% protein content, 1.449% fat content and 20.424% carbohydrate content, while the nutritional content of Black Sea Cucumber (Holothuria Edulis) was 6.214% water content, 18.864% ash content, 51.636% protein content, 0.659% fat content and 28.841% carbohydrate content.

Biochemical Analysis of Fruiting Bodies of Cordyceps Militaris Grown on Brown Rice

Cordyceps militaris is a medicinal fungus known for its diverse biofunctional properties. This subject has been extensively analyzed over the past decade. The diverse pharmacological potential of C. militaris has garnered significant global interest recently. This research aims to determine the proximate composition of in vitro cultivated C. militaris fruit bodies. The analysis of proximate composition included measurements of crude protein, fat, crude fiber, ash, total carbohydrate, and moisture content. The research focused on quantifying the overall nutritional value of the fruit bodies and evaluating biological efficiency, in addition to key bioactive compounds including cordycepin, ergosterol, and Vitamin D2. The results demonstrated that the carbohydrate content constituted 47.67% of dry weight, the protein content was 30.41%, and the fiber content accounted for 15%. The fruit bodies contained 39.82 mg/kg of cordycepin and 61.73 mg/100g of ergosterol, whereas vitamin D2 was absent in the cordyceps mushroom sample. The findings demonstrate that in-vitro-cultivated C. militaris fruit bodies exhibit considerable nutritional and therapeutic potential.

PENGARUH PENGGUNAAN KOAGULAN ALAMI SARI BELIMBING WULUH PADA TAHU PUTIH BERDASARKAN PERBEDAAN KONSENTRASI DAN LAMA PENGGUMPALAN

Tofu is a food whose process involves agglomerating soybean curd using coagulants, one of which is vinegar. In addition, from the other side, vinegar acid has shortcomings so that a natural coagulant substitute such as starfruit wuluh is needed. Belimbing wuluh one of the fruits that contains citric acid so that it can be used as a natural coagulant to replace vinegar, besides that it also contains oxalic acid, acetic acid, malic acid, formic acid, ascorbic acid and other compound components. This study aims to determine the effect of star fruit juice concentration and clotting time on the physicochemical and organoleptic properties of white tofu using star fruit as a coagulant. The method used was Randomized Group Design (RGD) using two factors, namely the concentration of Wuluh starfruit juice (factor 1) consisting of 4%, 5% and 6% and the coagulation activity (factor 2) consisting of 20 minutes, 30 minutes and 40 minutes. The testing parameters include protein content, fat content, moisture content, ash content, carbohydrate content, chewiness and organoleptic (scent, taste, color, texture and liking). The results of the analysis showed that there was an effect of the use of star fruit juice concentration and clotting time on protein in white tofu, which ranged from 3.50% - 6.77%; the range of water content ranged from 85.79% - 89.89%, chewiness was 3.08 N - 16.03 N. However, there was no significant effect on ash, fat and carbohydrates. As for the organoleptic test, the results showed a significant effect for scent parameters ranging from 2.80 (somewhat fragrant) - 4.05 (fragrant), color ranging from 2.45 (not bright white) - 3.45 (slightly bright white), texture ranging from 2.85 – 3,65 (slightly chewy) and liking ranging from 3.05 (slightly like) - 3.7 (like).

Structural Characterization and Bioactive Compound Evaluation of Fruit and Vegetable Waste for Potential Animal Feed Applications

Agricultural waste from the fruit and vegetable industry is used as an alternative source of animal feed, but detailed investigations are required. The aim of this work was to conduct a physico-chemical characterization, through analytical techniques, of fruit and vegetable wastes such as those of golden apples, red apples, carrots, celery, beetroots, and red potato peels. The bioactive compounds in the samples indicated a high carbohydrate content of 50.38 g/100 g in golden apples and 59.38 mg/100 g of organic acids in celery. In addition, the total phenolic content (TPC, mg gallic acid equivalent/g dry weight) varied between 3.72 in celery and 15.51 in beetroots. The antioxidant capacity values were significant. A thermal analysis showed thermal stability and weight loss, underscoring the composition of the solid samples. An infrared spectroscopy (FTIR) analysis showed C-H, O-H, C=O, and N-H functional groups in non-starchy carbohydrates, organic acids, and proteins. Microscopic techniques revealed the microstructure, particle size, and semicrystalline profile of the samples. The ultrastructure (determined via atomic force microscopy (AFM)) of celery consisted of a smooth and uniform surface with a lignin and cellulose texture. These results highlight the importance of fruit and vegetable waste as an alternative source of essential nutrients and bioactive compounds for animal feed.

Macronutrient concentrations in human milk beyond the first half year of lactation: a cohort study

ObjectiveHuman milk composition is dynamic. While extensive research has focused on its macronutrient concentrations during the first 6 months of lactation, limited research exists for extended lactation periods. This study...

Preliminary Evaluation of Watermelon Liquid Waste as an Alternative Substrate for Microalgae Cultivation: A Circular Economy Approach to the Production of High-Value Secondary Products by Chlorella vulgaris, Scenedesmus sp., Arthrospira platensis, and Chlamydomonas pitschmanii

In Italy, watermelon cultivation spans 9510 hectares, with production levels largely influenced by seasonal market demand. As a result, surplus watermelon left unsold by September often remain in the fields, where they decompose naturally and go to waste. A chemical analysis of the watermelon liquid fraction waste (WW) indicates a high carbohydrate concentration, highlighting the potential for biotechnological valorization of this waste stream, converting it into lipids or exopolysaccharides (EPSs). This study investigates the feasibility of utilizing WW as an alternative growth substrate for microalgae, aligning with circular economy principles and advancing sustainable agricultural practices. By repurposing agricultural byproducts, this research supports biorefinery objectives, aiming to convert biomass into high-value secondary products, including biofuels, pigments, and nutraceuticals. Scenedesmus and Chlorella strains demonstrated promising growth and adaptability in WW, achieving biomass yields of 0.95 ± 0.07 g L−1 and 0.37 ± 0.02 g L−1, respectively, with a significant EPS production observed as medium gelation. Although lipid accumulation was limited in this case by the WW substrate, the lipid profiles of both strains were distinctively altered, notably lacking linolenic acid.

Biomass Accumulation, Contaminant Removal, and Settling Performance of Chlorella sp. in Unsterilized and Diluted Anaerobic Digestion Effluent

Microalgae demonstrate significant efficacy in wastewater treatment. Anaerobic digestion effluent (ADE) is regarded as an underutilized resource, abundant in carbon, nitrogen, phosphorus, and other nutrients; however, the presence of inhibitory factors restricts microalgal growth, thereby preventing its direct treatment via microalgae. The purpose of this study was to dilute ADE using various dilution media and subsequently cultivate Chlorella sp. to identify optimal culture conditions that enhance microalgal biomass and water quality. The effects of various dilution conditions were assessed by evaluating the biomass, sedimentation properties, and nutrient removal efficiencies of microalgae. The results demonstrate that microalgal biomass increases as the dilution ratio increased. The microalgae biomass in the treatments diluted with simulated wastewater was significantly higher than that with deionized water, but their effluent quality failed to meet discharge standards. The treatment diluted with deionized water for 10 times exhibited abundant microbial biomass with strong antioxidant properties. The corresponding total phosphorus concentration in the effluent (6.96 mg/L) adhered to emission limits under the Livestock and Poultry Industry Pollutant Emission Standards (8 mg/L), while ammonia nitrogen concentration (90 mg/L) was near compliance (80 mg/L). The corresponding microbial biomass, with a sludge volume index (SVI30) of 72.72 mL/g, can be recovered economically and efficiently by simple precipitation. Its high protein (52.07%) and carbohydrate (27.05%) content, coupled with low ash (10.75%), makes it a promising candidate for animal feed and fermentation. This study will aid in understanding microalgal growth in unsterilized ADE and establish a theoretical foundation for cost-effective ADE purification and microalgal biomass production.

Microstructural optimization of lignin‐based carbon fiber: Effects of purification and high‐temperature heat treatment

AbstractLignin is a renewable biomass polymer with a carbon content of more than 60% and has been extensively studied for making lignin‐based carbon fibers (CFs). However, impurities and high carbohydrate content in lignin materials severely limit their molding in melt spinning, and pre‐oxidation and carbonization processes also affect the subsequent preparation of CFs. This paper presents a detailed study of lignin‐based carbon fiber preparation. Pretreatment increased lignin's decomposition temperature to 302.1°C and enabled melt‐spun fiber preparation. Then, pre‐oxidation and carbonization of lignin fibers were further discussed. The pre‐oxidized fibers prepared at 280°C and 0.4°C min−1 have high thermal stability and excellent carbonization performance. The lignin‐based carbon fibers (LCFs) prepared at 1100°C and 3°C min−1 have the highest degree of graphitization, and the surface is smooth without obvious holes. Under these conditions, LCFs have a graphitization degree of 1.50 and conductivity of 62.50 S cm−1, making them suitable for sensors and capacitors.Highlights Effects of pretreatment on lignin structure and properties have been studied. Effects of pre‐oxidation and carbonization on the lignin fibers were studied. Lignin‐based carbon fibers with high conductivity were obtained.

Bioenergy products sequestration proportions among three mixotrophically cultivated microalgae by remediating two organic waste resources

In this study, three microalgae species were cultivated using dairy and fish wastewater: Haematococcus pluvialis, Coelastrella saipanensis, and Chlorella sp. The process involved manipulating various physicochemical conditions, to determine optimal growth parameters. Our evaluation considered cell count, biomass productivity, specific growth rate, pigments, carbohydrates, proteins, lipid compositions, and cellulose stored in microalgae. A significant observation of highest cellulose accumulation was recorded in C. saipanensis cultivated in dairy waste (DW) medium (2.54 ± 0.042 µg/mg). In contrast, the species grown in fish waste (FW) media recorded a lower level (0.9405 ± 0.06 µg/mg) of cellulose. In DW, H. pluvialis and C. saipanensis accumulated substantial amounts of astaxanthin and carotenoid, respectively. Carbohydrate, protein, and lipid accumulation was maximized in DW culture, with H. pluvialis exhibiting a more incredible carbohydrate content. Lipid analysis showed as Chlorella sp. was capable of accumulating alpha-linolenic acid. The disparity may be attributed to DW’s nutritional and mineral content, which encourages cellulose deposition. The FTIR analysis confirmed the accumulation of cellulose. These findings underscore the potential of DW and FW media as valuable resources for microalgal biofuel and ethanol production, offering a hopeful future for sustainable energy production.

Potential utilization of paddy straw and coconut coir for the cultivation of Pleurotus eous: effects on nutritional composition and antioxidant capacity

Oyster mushrooms (Pleurotus species), which are valued for their medicinal and nutritional properties, are the predominantly cultivated mushroom variety in Sri Lanka. The most commonly used substrate to cultivate Pleurotus species in Sri Lanka is sawdust. However, due to the availability of many other agricultural wastes in Sri Lanka, the aim of this study was to determine which substrate from paddy straw (PS), coconut coir (CC), and combinations of PS and CC in the ratios of 50 PS:50 CC, 75 PS:25 CC, and 25 PS:75 CC would be the best to grow Pleurotus eous. The weight of each mushroom harvested was measured to determine the highest yield. Lowry and phenol-sulphuric assays were carried out to determine the total protein and total carbohydrate content respectively. DPPH and phophomolybdenum assays were used to analyse the antioxidant activity. The total phenolic content was determined using the Folin-Ciocalteu method. All mushrooms tested positive for qualitative tests of terpenoids, steroids, saponins and polyphenols. 100% PS produced mushrooms with the highest yield (34.72 ± 5.23 g). 50 PS:50 CC produced mushrooms with a significantly high total protein content (5.28 ± 0.24 g/100 g) and total carbohydrate content (31.12 ± 5.64 g/100 g). They also had a high total antioxidant capacity (4.51 ± 0.07 mg AAE/g) and total phenolic content (5.45 ± 0.34 mg GAE/g). Therefore, to cultivate Pleurotus eous, the 50 PS:50 CC combination can be utilized. Based on the mushroom yield, 100% PS can also be recommended for cultivation of Pleurotus eous.

Comparative Study of the Nutritive and Elemental Composition of Smoked and Raw Clarias gariepinus from Three Selected Fish Farms in Ado Ekiti, Ekiti State, Nigeria

Clarias gariepinus is of economic importance in aquaculture due to its rapid growth, omnivorous diet, and potential as food source. It has been widely cultured and dispensed both in raw and smoked forms in various regions to meet the demand for fish protein This study assessed and compared the nutritional quality and elemental composition of smoked and raw samples of Clarias gariepinus. A total of twelve fish samples were analysed, six raw samples and six smoked samples with two fishes sampled from each farm tagged A, B and C. The Proximate and elemental analysis were done using standard procedures. The result of the study showed that moisture content measured in the raw Clarias gariepinus which ranged between (73.074 ± 1.59) % and (71.52 ± 1.83) %. was higher than that of the smoked fish ranging from (26.69±0.03) % to (32.21± 0.02) %. The protein content of smoked fish ranged (43.2±0.02) % to (46.78±0.01) while that of raw fish was between (20.52 ± 1.12) % and (22.15 ± 0.43) %. Similarly, the crude fat, ash, carbohydrate, and fibre content were higher in the smoked fish than raw catfish samples. The elemental composition of both smoked and raw catfish were within the permissible limit of World Health Organization. Arsenic was not detected in the muscle of the raw fish, while cadmium, chromium, lead and magnesium were present in values within WHO permissible limit and similar results was observed for the smoked fish samples. The result of this study presents the possibility of smoked catfish being more nutritious than the raw samples aside from the fact that it has more shelf life than raw fish. Also, both smoked and raw samples of the fish are safe as the values of heavy metal observed were all within the permissible limit of WHO.

Evaluation of stevia leaf (<em>Stevia rebaudiana</em> Bertoni) powder as sugar-substitute in cashew nut fortified cookies

Stevia rebaudiana is an herbal supplement used as a substitute for sugar in food processing. Considering the potentials of stevia as a zero-calorie-natural sweetener, this study aimed to assess the antioxidant and anti-hyperglycemic properties of stevia (Stevia rebaudiana Bertoni) leaf powder (SLP) to find its suitability to formulate low-calorie foods. Extracts of SLP obtained with hexane, ethyl acetate, and methanol were evaluated for DPPH, ABTS+, FRAP and enzyme inhibitory assays. Adopting a standard recipe, five different cookie types were prepared by incorporating varying proportions of SLP and evaluated by a 30 semi-trained panel. The nutritional composition and shelf-life stability were also evaluated. SLP had moisture (8.0 %), ash (13.5 %), crude protein (12.4 %), crude fat (6.9 %), crude fiber (18.3 %), and carbohydrate (40.9 %). Sensory results showed that the cookie formulated with the lowest amount of SLP (T1= 2.0g) had the highest overall acceptability with the highest preference for appearance and smell. The cookie type formulated with the highest amount of SLP (T4= 8.0g) had the highest crude protein (13.50%), crude fiber (21.76%), and lowest carbohydrate contents (40.88%). The keeping quality of the cookies remained acceptable within the storage period. These results suggest that acceptable quality cashew nut fortified cookies could be prepared by replacing sucrose with stevia leaf powder

Biovalorization of whey waste as economic nutriment for mycogenic production of single cell oils with promising antibiofilm and anticancer potentiality

The production of value-added bio-compounds from rejuvenated sources and their recruitment for healthcare services are paramount objectives in the agenda of white biotechnology. Hereupon, the current study focused on economic production of single cell oils (SCOs) from oleaginous fungi Alternaria sp. (A-OS) and Drechslera sp. (D-OS) using cheese whey waste stream, followed by their evaluation as antibiofilm and anticancer agents, for the first time. As a sole substrate for growth, the whey aided in lipid accumulation by 3.22 and 4.33 g/L, which representing 45.3 and 48.2% lipid content in Drechslera sp. (D-OS) and Alternaria sp. (A-OS), respectively. Meanwhile, a higher unsaturation degree was detected in A-OS by 62.18% comparing to 53.15% of D-OS, with advantageous presence of omega-6 poly unsaturated fatty acid by 22.67% and 15.04% for A-OS and D-OD, respectively, as revealed by GC-MS and FTIR characterization analysis. Interestingly, an eminent and significant (P ≤ 0.05) antibiofilm potency was observed in a dose-dependent modality upon employing both SCOs as antibiofilm agents. Whereas, 100 µg/mL of A-OS recorded superior inhibition of P. aeruginosa, S. aureus and C. albicans biofilms development by 84.10 ± 0.445, 90.37 ± 0.065 and 94.96 ± 0.21%, respectively. Whereas, D-OS (100 µg/mL) thwarted the biofilms of P. aeruginosa, S. aureus and C. albicans by 47.41 ± 2.83, 62.63 ± 5.82 and 78.67 ± 0.23%, correspondingly. Besides, the metabolic performance of cells within biofilm matrix, protein, carbohydrate contents and hydrophobicity of examined biofilms were also curtailed in a significant correlation with biofilm biomass (r ≥ 0.9). Further, as anticancer agents, D-OS recorded higher potency against A549 and CaCo-2 cell lines with IC50 values of 2.55 and 3.425% and SI values of 10.1 and 7.5, respectively. However, A-OS recorded 8.275% and 2.88 for IC50 and SI of Caco-2 cells, respectively. Additionally, A-OS activated caspase 3 by 64.23 ± 1.18% and 53.77 ± 0.995% more than D-OS (52.09 ± 0.222% and 49.72 ± 0.952%) in A549 and Caco-2 cells, respectively. Furthermore, the enzymes, which associated with cancer invasion, metastasis, and angiogenesis (i.e., MMP2 and MMP9) were strongly inhibited by A-OS with 18.58% and 8.295%, respectively as IC50 values; while D-OS results recorded 23.61% and 13.16%, respectively, which could be ascribed to the higher ω-6/ω-3 contents of A-OS. The promising results of the current study opens up the vision to employ SCOs as anti-infective nutraceuticals and in complementary/alternative therapy and prophylactic programs as well.

Antioxidant Properties of Freeze Dried Mix Fruits and Vegetables Product ‘Miss Freezy’ Using Lyophilization Method

Background: The proper processing of fruits and vegetable-based food products will improve appearance, taste, bioactivities, and preservation. Freeze-dried (FD) or lyophilization of foods extends shelf life and preserves food's nutritional value and bioactivities. The objective of this study is to compare the antioxidant properties, nutritional value, and vitamins of fresh fruit-vegetables and the combined FD product. Methods: A combination of fruits (mango, papaya, star-fruit, guava, banana, bengkuang), and vegetables (cucumber), called “Miss Freezy” were cleaned with an ozonizer and processed using the lyophilization method. The antioxidant activities measured included 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and Hydrogen Peroxide (H2O2) assay. While the proximate analysis measured was the determination of water content, ash content, fat content, protein and carbohydrate content, and vitamin C. Results: The value of antioxidant activity in fruit-vegetable combination FD products was higher than in fresh fruit. The median inhibition concentration (IC50) of DPPH, ABTS, and H2O2 scavenging activities were guava 48.74 µg/ml, guava 128.39 µg/ml, and banana 155.55 µg/ml, respectively. The results showed that the water content of fresh fruits, and vegetables was greater than FD products and inversely proportional to the level of ash, fat, protein, carbohydrates, and vitamin C lower than FD products. Conclusions: Based on the results of antioxidant activities, proximate analysis and vitamin C of the FD product was higher than fresh fruits and vegetables. The FD fruit-vegetable combination product can be used as an alternative snack product that is practical and healthy.

Characteristics of Buffalo Milk Curd on Variations in Fermentation Time

Curd is a processed product from buffalo milk through a fermentation process. The clumping of fresh buffalo milk into curd occurs due to the presence of Lactic Acid Bacteria (LAB) in buffalo milk. Curd contains lactic acid bacteria (LAB), which are beneficial for health. These bacteria not only extend the shelf life of food by inhibiting the growth of spoilage and pathogenic bacteria but also produce bioactive compounds that are good for the body. LAB contained in curd even has probiotic properties. This research used a starter of 10% buffalo milk curd. In one production, 4500 ml of fresh buffalo milk and 500 ml of starter are used, yielding 3.8 kg of buffalo milk curd. The results of observations during 7 days of fermentation were water content (81.25-72.53%), ash content (0.93-0.89%), protein content (8.97-7.94%), fat content (7 .72-7.10%), carbohydrate content (1.13-11.54%), pH value (5.72-4.27), temperature (26.87-28.43oC), Total Lactic Acid Bacteria (1.6x109- 8.1x109 cfu/ml).

Effect of roasting on physicochemical, functional, thermal, pasting, and structural properties of tamarind seed powder

The impact of different roasting conditions on the nutritional profile, physical, functional, thermal, pasting properties, morphology, crystallinity, and functional groups of tamarind seed powder (TSP) was investigated. Roasting treatment enhanced ash, carbohydrate, fat, and flavonoid content (2.21 to 11.93 mg QE/g) while significantly reduced protein, fiber, phenol content, tannin (2.72 to 1.36 mg TAE/g), antioxidant activity, and physical characteristics like bulk density, tap density. The concentrations of minerals like Mg, K, Ca, and P (3006.00, 8313.85, 482.44, 2723.78 mg/kg) and essential and non-essential amino acids were significantly enhanced by roasting. The roasted TSP exhibited a significant difference in pasting and functional properties compared to the control powder. The proportion of crystallinity in the roasted powder decreased according to the XRD patterns. The SEM micrographs revealed slight modifications in the morphology of TSP after roasting. According to our outcomes, the enhanced nutritional content of roasted TSP has excellent potential for usage as a food ingredient with added value.