On-demand ride-pooling systems have gained increasing attention in science and practice in recent years. Simulation studies have shown an enormous potential to reduce fleet sizes and vehicle kilometers traveled if private car trips are replaced with ride-pooling services. However, existing simulation studies assume operation with autonomous vehicles, with no restrictions on operational tasks required when the vehicles are operated by manual drivers. In this article, we simulate and evaluate the operational challenges of non-autonomous ride-pooling systems through driver shifts and breaks and compare their capacity and efficiency to autonomous on-demand services. Based on the existing ride-pooling service MOIA in Hamburg, Germany, we introduce shift and break schedules and implement a new hub return logic to perform the respective tasks at different types of vehicle hubs. This way, currently operating on-demand services are modeled more realistically and the efficiency gains of such services through autonomous vehicles are quantified. The results suggest that operational challenges substantially limit the ride-pooling capacity in terms of served rides with a given number of vehicles. While results largely depend on the chosen shift plan, the presented operational factors should be considered for the assessment of current operational real-world services. The contribution of this study is threefold: From a technical perspective, it is shown that the explicit simulation of operational constraints of current services is crucial to assess ride-pooling services. From a policy perspective, the study shows the operational challenges of a ride-pooling service with non-autonomous vehicles and the potential of future autonomous services. Lastly, the paper adds to the literature a practical ride-pooling simulation use case based on observed real-world demand and shift data.