With the rapid development of the automobile industry, automobile manufacturers begin to pay more attention to NVH (Noise, Vibration, Harshness). The sound of car closing the door is one of the characteristics of NVH. Therefore, it is of great significance to analyze and improve the door closing sound quality of cars to improve the comprehensive performance of automobile. The dynamic characteristics of car door closing can indirectly reflect the sound quality of car door closing. In this paper, firstly, a finite element model of a quarter of car body with the complete structure of door, window and sealing system was established by HyperMesh software. Secondly, stress-strain tests of sealing strip were performed, and their data were input into the hyperelastic material setting in ABAQUS for selecting the most suitable strain energy function. Thirdly, the sealing strip model was introduced into ABAQUS, a simulation of reaction force and compression displacement on the sealing strip was performed by ABAQUS to generate the sealing strip CLD curve, and be verified by test. Fourthly, the finite element model was introduced into ABAQUS, the Y-direction (rightward direction from driver) acceleration of the three specified points at door closing, such as the upper corner of the door, the midpoint of the window and the body points on the B-pillar, were studied numerically. Finally, their Y-direction jitter acceleration were studied numerically and experimentally. The results showed that the relative error between calculated and experimental maximum values of Y-direction jitter acceleration at three specified points at door closing is less than 5.5%, and the acceleration attenuation trend is consistent with the test.