This study deals with the analysis of the heat and velocity profile of the fractional-order Oldroyd-B bio-nanofluid within a bounded channel. The study has a wide range of scope in modern fields of basic science such as medicine, the food industry, electrical appliances, nuclear as well as industrial cooling systems, reducing pollutants, fluids used in the brake systems of vehicles, etc. Oldroyd-B fluid is taken as a bio-nanofluid composed of base fluid (blood) and copper as nanoparticles. Using the fractional-order Oldroyd-B parameter, the governing equation is generalized from an integer to a non-integer form. A strong approach, i.e., a finite difference scheme, is applied to discretize the model, because the fractional approach can well address the physical phenomena and memory effect of the flow regime. Therefore, a Caputo fractional differentiation operator is used for the purpose. The transformations for the channel flow are utilized to transfigure the fractional-order partial differential equations (PDEs) into non-dimension PDEs. The graphical outcomes for non-integer ordered Oldroyd-B bio-nanofluid dynamics and temperature profiles are navigated using the numerical technique. These results are obtained under some very important physical conditions applied as a magnetic field effect, variable thermal conductivity, permeable medium, and heat source/sink. The results show that the addition of (copper) nanoparticles to (blood) base fluids enhances the thermal conductivity. For a comparative study, the obtained results are compared with the built-in results using the mathematical software MAPLE 2016.