Capture Of Nanoparticles Research Articles

Temporary suppression the sequestrated function of host macrophages for better nanoparticles tumor delivery

Orchestration of nanoparticles to achieve targeting has become the mainstream for efficient delivery of antitumor drugs. However, the low delivery efficiency becomes the biggest barrier for clinical translation of cancer nanomedicines, as most of them are sequestrated in the liver where more macrophages located in are responsible for capture of systemic administrated nanoparticles. In this study, we found that the depletion of the liver macrophages could lead to a superior improvement in the nanoparticles delivery. Firstly, we developed clodronate-containing liposomes (clodrolip) to transiently suppress the phagocytic function of macrophages, the residual macrophages in liver only accounted for less than 1% when the mice were treated with clodrolip in advance. In addition, the pharmacokinetics results of treatment with paclitaxel-poly(lactic-co-glycolic acid) (PTX-PLGA) nanoparticles disclosed that the AUC of PTX in the macrophages depletion group increased 2.11-fold. These results meant that the removal of macrophages would decrease the nanoparticles accumulation in the liver and better the biodistribution and bioavailability of nanoparticles delivery systems. Moreover, treatment of mice with melanoma by the combination of clodrolip and PTX-PLGA nanoparticles resulted in an elevated anti-tumor efficacy, the tumor inhibition ratio was nearly reached to 80%. Furthermore, these combinatorial regimens have demonstrated negligible toxicity in incidence of adverse effects. In conclusion, the encouraging results from this study inspire the generation of a rational strategy to focus on microenvironmental priming for modulation of innate immunity and to improve delivery efficiency of nanoparticles.

Isolation of nematode DNA from 100 g of soil using Fe3O4 super paramagnetic nanoparticles

An economical method for extracting nematode DNA from 100 g of soil was developed to facilitate nematode detection and quantification, and tested using the Northern root-knot nematode,Meloidogyne hapla. The method utilised enzymatic laundry detergent lysis, Fe3O4super paramagnetic iron oxide nanoparticle (SPION) capture, and polyvinylpolypyrrolidone (PVPP) purification. Resultant DNA from this SPION capture method was approximately 100-fold less but of similar quality to DNA obtained from a standard phenol procedure and a commercial DNA extraction kit. An addition of 10 mg of nanoparticles to the extraction lysate was identified to maximise DNA yield while minimising co-capture of contaminants. The detection limit of the SPION capture method was approximately 100 nematodes (100 g soil)−1. The SPION capture method extracted nematode DNA from mineral soils but requires further optimisation for extraction from high organic matter (i.e., ‘muck’) soils. The benefits of this method compared to alternative techniques are discussed.

Filtration performance of electrospun acrylonitrile-butadiene elastic fiber mats in solid aerosol filtration

Abstract Elastic acrylonitrile-butadiene copolymer fibers were electrospun to fabricate fiber mats of five different basis weights: 10, 20, 30, 45, and 60 g/m2. The fiber mats were investigated for properties of pore diameter and permeability. As the basis weight increased, bubble point and mean flow pore diameters as well as the permeability/mat-thickness values decreased. The fiber mats were tested as filter media for capture of NaCl nanoparticles from aerosol flow. Due to the elastic nature of the fibers, the mats were tested in stretched and non-stretched conditions to evaluate changes in filter performance. The pressure drop and penetration of the filter media were calculated using correlations from literature and by assuming idealized properties of constant mat thickness or constant mat volume when the mat is stretched. Experimentally, the fiber mats of higher basis weights yielded higher pressure drops and lower penetrations compared to lower basis weight mats. The stretched fiber mats showed decreased pressure drops and increased particle penetrations compared to the non-stretched mats due to the enlargement of pores within the mats. The FI values were about the same between stretched and non-stretched mats of same starting basis weights.

Functional Paper-Based Platform for Rapid Capture and Detection of CeO2 Nanoparticles

Development of systems for capture, sequestration, and tracking of nanoparticles (NPs) is becoming a significant focus in many aspects of nanotechnology and environmental research. These systems enable a broad range of applications for evaluating concentration, distribution, and effects of NPs for environmental, clinical, epidemiological, and occupational exposure studies. Herein, we describe the first example of a ligand-graft multifunctional platform for capture and detection of cerium oxide (CeO2 or ceria) NPs. The approach involves the use of redox-active ligands containing o-dihydroxy functionality, enabling multivalent binding, surface retention, and formation of charge transfer complexes between the grafted ligand and the NPs. Using this strategy, paper-based and microarray-printed platforms with NP-capture ability involving either catechol or ascorbic acid as ligands were successfully fabricated. Surface modification was determined by infrared spectroscopy, electron microscopy, X-ray spectroscopy, and thermogravimetric analysis. Functionality was demonstrated for the rapid assessment of NPs in chemical mechanical planarization (CMP) slurries and CMP wastewaters. This novel approach can enable further development of devices and separation technologies including platforms for retention and separation of NPs and measurement tools for detection of NPs in various environments.

Rapid and sensitive detection of microRNA via the capture of fluorescent dyes-loaded albumin nanoparticles around functionalized magnetic beads

MicroRNAs (miRNAs) play important roles in gene regulation and cancer development. Nowadays, it is still a challenge to detect low-abundance miRNAs. Here, we present a magnetic fluorescent miRNA sensing system for the rapid and sensitive detection of miRNAs from cell lysates and serum samples. In this system, albumin nanoparticles (Alb NPs) were prepared from inherent biocompatible bovine serum albumin (BSA). A large number of fluorescent dyes were loaded into Alb NPs to make Alb NPs serve as signal molecular nanocarriers for signal amplification. Benefited from the reactive functional groups-carboxyl groups of Alb NPs, p19 protein, a viral protein that can bind and sequester short RNA duplex effectively and selectively, was modified successfully to the surface of the fluorescent dyes-loaded Alb NPs, thus enabling the probe:target miRNA duplex recognition and binding. Followed by the introduction of gold nanoparticles coated magnetic microbeads (Au NPs-MBs), which were prepared through a novel and simple method, the system combined the merits of the rapid and efficient collection given by MBs with the good affinities to attach probe molecules endowed by the coated gold layer. A broad linear detection range of 10fM–10nM and a low detection limit of 9fM were obtained within 100min by detecting a model target miRNA-21. The feasibility of this method for rapid and sensitive quantification might advance the use of miRNAs as biomarkers in clinical praxis significantly.

Collisions of Ir Oxide Nanoparticles with Carbon Nanopipettes: Experiments with One Nanoparticle.

Investigating the collisions of individual metal nanoparticles (NPs) with electrodes can provide new insights into their electrocatalytic behavior, mass transport, and interactions with surfaces. Here we report a new experimental setup for studying NP collisions based on the use of carbon nanopipettes to enable monitoring multiple collision events involving the same NP captured inside the pipet cavity. A patch clamp amplifier capable of measuring pA-range currents on the microsecond time scale with a very low noise and stable background was used to record the collision transients. The analysis of current transients produced by oxidation of hydrogen peroxide at one IrOx NP provided information about the origins of deactivation of catalytic NPs and the effects of various experimental conditions on the collision dynamics. High-resolution TEM of carbon pipettes was used to attain better understanding of the NP capture and collisions.

Immobilization of nanoparticles by occlusion into microbial calcite

Binding of nanoparticles (NPs) to mineral surfaces influences their transport through the environment. The potential, however, for growing minerals to immobilize NPs via occlusion (the process of trapping particles inside the growing mineral) has yet to be explored in environmentally relevant systems. In this study, the ureolytic bacteria Sporosarcina pasteurii was used to induce calcium carbonate precipitation in the presence of organo-metallic manufactured nanoparticles. As calcite crystals grew the nanoparticles in the solution became trapped inside these crystals. Capture of NPs within the calcite via occlusion was verified by transmission electron microscopy of thin foils. Nanoparticles with a negative surface charge were captured with greater efficiency than those with a positive surface charge, resulting from stronger attachment of negative nanoparticles to the positively charged calcite surfaces, which in turn facilitated occlusion. Thermodynamic and kinetic analysis, however, did not reveal a significant difference in kp (calcite precipitation rate constant) or the critical saturation at which precipitation initiates (Scrit), indicating the presence of different charged nanoparticles did not influence calcite precipitation at the concentrations used here. Overall, these findings demonstrate that microbially driven mineral precipitation has potential to immobilize nanoparticles in the environment via occlusion.

Peptide engineered microcantilevers for selective chemical force microscopy and monitoring of nanoparticle capture.

Engineered peptides capable of binding to silica have been used to provide contrast in chemical force microscopy and tested for their capacity to selectively capture silica nanoparticles (NPs). Gold coated atomic force microscopy (AFM) microcantilevers with integrated tips and colloidal probes were functionalized with engineered peptides through a thiol group of a terminal cysteine which was linked via a glycine trimer to a 12-mer binding sequence. The functionalized probes demonstrated a significantly increased binding force on silicon oxide areas of a gold-patterned silicon wafer, whereas plain gold probes, and those functionalized with a random permutation of the silica binding peptide motif or an all-histidine sequence displayed similar adhesion forces to gold and silicon oxide. As the functionalized probes also allowed contact mode imaging subsequently to the adhesion mapping, also the associated friction contrast was measured and found to be similar to the adhesion contrast. Furthermore, the adsorption of silica NPs onto planar gold surfaces functionalized in the same manner was observed to be selective. Notably, the surface coverage with silica NPs was found to decrease with increasing pH, implying the importance of electrostatic interactions between the peptide and the NPs. Finally, the adsorption of silica NPs was monitored via the decrease in fundamental resonance frequency of an AFM microcantilever functionalized with silica binding peptides.

Trapping magnetic nanoparticles for in-line capillary electrophoresis in a liquid based capillary coolant system

A method to trap magnetic nanoparticles inside the electrophoretic capillary has been developed without disabling and disturbing the coolant liquid recirculation. The complete optimization from setting up of the external magnetic field to injecting the magnetic nanoparticles in order to facilitate efficient trapping is described. In addition to this, the setup has been tested with 150mM sodium phosphate buffer as the background electrolyte at different pH values (2, 4, 6 and 8). All these pH values have been investigated at 25°C and 37°C to mimic room temperature and physiological temperature, respectively. The capture of magnetic nanoparticles was examined via UV detection and also microscopically. To demonstrate the efficiency of the trapping, an in-line capillary electrophoresis enzymatic assay using hFMO3 was performed successfully. This method can be of further use for in-line capillary electrophoresis using magnetic nanoparticles as a solid support for enzyme/protein immobilization.

Optimization of an endovascular magnetic filter for maximized capture of magnetic nanoparticles.

To computationally optimize the design of an endovascular magnetic filtration device that binds iron oxide nanoparticles and to validate simulations with experimental results of prototype devices in physiologic flow testing. Three-dimensional computational models of different endovascular magnetic filter devices assessed magnetic particle capture. We simulated a series of cylindrical neodymium N52 magnets and capture of 1500 iron oxide nanoparticles infused in a simulated 14mm-diameter vessel. Device parameters varied included: magnetization orientation (across the diameter, "D", along the length, "L", of the filter), magnet outer diameter (3, 4, 5mm), magnet length (5, 10mm), and spacing between magnets (1, 3mm). Top designs were tested in vitro using 89Zr-radiolabeled iron oxide nanoparticles and gamma counting both in continuous and multiple pass flow model. Computationally, "D" magnetized devices had greater capture than "L" magnetized devices. Increasing outer diameter of magnets increased particle capture as follows: "D" designs, 3mm: 12.8-13.6%, 4mm: 16.6-17.6%, 5mm: 21.8-24.6%; "L" designs, 3mm: 5.6-10%, 4mm: 9.4-15.8%, 5mm: 14.8-21.2%. In vitro, while there was significant capture by all device designs, with most capturing 87-93% within the first two minutes, compared to control non-magnetic devices, there was no significant difference in particle capture with the parameters varied. The computational study predicts that endovascular magnetic filters demonstrate maximum particle capture with "D" magnetization. In vitro flow testing demonstrated no difference in capture with varied parameters. Clinically, "D" magnetized devices would be most practical, sized as large as possible without causing intravascular flow obstruction.

Asymmetric Peptide Nanoribbons.

Asymmetry in chemical structure or shape at molecular, nanoscale, or microscopic levels is essential to a vast number of functionalities in both natural and artificial systems. Bottom-up approaches to create asymmetric supramolecular nanostructures are considered promising but this strategy suffers from the potentially dynamic nature of noncovalent interactions. We report here on supramolecular self-assembly of asymmetric peptide amphiphiles consisting of two different molecularly linked domains. We found that strong noncovalent interactions and a high degree of internal order among the asymmetric amphiphiles lead to nanoribbons with asymmetric faces due to the preferential self-association of the two domains. The capture of gold nanoparticles on only one face of the nanoribbons demonstrates symmetry breaking in these supramolecular structures.

Integration of microcoils for on-chip immunosensors based on magnetic nanoparticles capture

Immunoassays using magnetic nanoparticles (MNP) are generally performed under the control of permanent magnet close to the micro-tube of reaction. Using a magnet gives a powerful method for driving MNP but remains unreliable or insufficient for a fully integrated immunoassay on lab-on-chip. The aim of this study is to develop a novel lab-on-chip concept for high efficient immunoassays to detect ovalbumin (Biodefense model molecule) with microcoils employed for trapping MNP during the biofunctionalization steps. The objectives are essentially to optimize their efficiency for biological recognition by assuring a better bioactivity (antibodies-ovalbumin), and detect small concentrations of the targeted protein (~10pg/mL). In this work, we studied the response of immunoassays complex function of ovalbumin concentration. The impact of MNP diameter in the biografting protocol was studied and permitted to choose a convenient MNP size for efficient biorecognition. We realized different immunoassays by controlling MNP in test tube and in microfluidic device using a permanent magnet. The comparison between these two experiments allows us to highlight an improvement of the limit of detection in microfluidic conditions by controlling MNP trapping with a magnet.

Functionalization of Fe₃O₄ NPs by Silanization: Use of Amine (APTES) and Thiol (MPTMS) Silanes and Their Physical Characterization.

In this paper the results concerning the synthesis of magnetite (Fe3O4) nanoparticles (NPs), their functionalization using silane derivatives, such as (3-Aminopropyl)triethoxysilane (APTES) and (3-mercaptopropyl)trimethoxysilane (MPTMS), and their exhaustive morphological and physical characterization by field emission scanning electron microscopy (FE-SEM) with energy dispersion X-ray spectrometer (EDX) analysis, AC magnetic susceptibility, UV-VIS and IR spectroscopy, and thermogravimetric (TGA) analyses are reported. Two different paths were adopted to achieve the desired functionalization: (1) the direct reaction between the functionalized organo-silane molecule and the surface of the magnetite nanoparticle; and (2) the use of an intermediate silica coating. Finally, the occurrence of both the functionalization with amino and thiol groups has been demonstrated by the reaction with ninhydrin and the capture of Au NPs, respectively.

Functional droplets that recognize, collect, and transport debris on surfaces.

We describe polymer-stabilized droplets capable of recognizing and picking up nanoparticles from substrates in experiments designed for transporting hydroxyapatite nanoparticles that represent the principal elemental composition of bone. Our experiments, which are inspired by cells that carry out materials transport in vivo, used oil-in-water droplets that traverse a nanoparticle-coated substrate driven by an imposed fluid flow. Nanoparticle capture is realized by interaction of the particles with chemical functionality embedded within the polymeric stabilizing layer on the droplets. Nanoparticle uptake efficiency is controlled by solution conditions and the extent of functionality available for contact with the nanoparticles. Moreover, in an elementary demonstration of nanoparticle transportation, particles retrieved initially from the substrate were later deposited "downstream," illustrating a pickup and drop-off technique that represents a first step toward mimicking point-to-point transportation events conducted in living systems.

Nanoparticles and the immune system: challenges and opportunities.

Nanoparticles and the immune system: challenges and opportunities.

Magnetic filtration of phase separating ferrofluids: From basic concepts to microfluidic device

In this work, we briefly review magnetic separation of ferrofluids composed of large magnetic particles (60nm of the average size) possessing an induced dipole moment. Such ferrofluids exhibit field-induced phase separation at relatively low particle concentrations (∼0.8vol%) and magnetic fields (∼10kA/m). Particle aggregates appearing during the phase separation are extracted from the suspending fluid by magnetic field gradients much easier than individual nanoparticles in the absence of phase separation. Nanoparticle capture by a single magnetized microbead and by multi-collector systems (packed bed of spheres and micro-pillar array) has been studied both experimentally and theoretically. Under flow and magnetic fields, the particle capture efficiency Λ decreases with an increasing Mason number for all considered geometries. This decrease may become stronger for aggregated magnetic particles (Λ∝Ma−1.7) than for individual ones (Λ∝Ma−1) if the shear fields are strong enough to provoke aggregate rupture. These results can be useful for development of new magneto-microfluidic immunoassays based on magnetic nanoparticles offering a much better sensitivity as compared to presently used magnetic microbeads.

Microfluidic separation of magnetic nanoparticles on an ordered array of magnetized micropillars.

Microfluidic separation of magnetic particles is based on their capture by magnetized microcollectors while the suspending fluid flows past the microcollectors inside a microchannel. Separation of nanoparticles is often challenging because of strong Brownian motion. Low capture efficiency of nanoparticles limits their applications in bioanalysis. However, at some conditions, magnetic nanoparticles may undergo field-induced aggregation that amplifies the magnetic attractive force proportionally to the aggregate volume and considerably increases nanoparticle capture efficiency. In this paper, we have demonstrated the role of such aggregation on an efficient capture of magnetic nanoparticles (about 80 nm in diameter) in a microfluidic channel equipped with a nickel micropillar array. This array was magnetized by an external uniform magnetic field, of intensity as low as 6-10kA/m, and experiments were carried out at flow rates ranging between 0.3 and 30μL/min. Nanoparticle capture is shown to be mostly governed by the Mason number Ma, while the dipolar coupling parameter α does not exhibit a clear effect in the studied range, 1.4 < α < 4.5. The capture efficiency Λ shows a strongly decreasing Mason number behavior, Λ∝Ma^{-1.78} within the range 32≤ Ma ≤ 3250. We have proposed a simple theoretical model which considers destructible nanoparticle chains and gives the scaling behavior, Λ∝Ma^{-1.7}, close to the experimental findings.

The role of mesopores in achieving high efficiency airborne nanoparticle filtration using aerogel monoliths

Abstract This study evaluates the role of small mesopore volume fraction in aerogel monoliths in achieving high efficiency airborne nanoparticle filtration. The diffusional flow in mesopores (diameter 2–50 nm) is more effective in nanoparticle capture than the viscous flow regime encountered in macropores (diameter > 50 nm). Substantial macropore fraction, however, is essential for achieving high air permeability. This idea is tested by evaluating the filtration efficiency of 25–150 nm diameter airborne sodium chloride nanoparticles and the permeability of air through hybrid aerogel monoliths with about 2–4% by volume mesopore content. The hybrid aerogels are prepared by growing discontinuous silica aerogel particles in the macropores of δ-form syndiotactic polystyrene (sPS). The results show that air permeability is not much affected by the silica content although the particle capture efficiency is increased to >99.95% due to the contribution of diffusional deposition aided by the mesopores. The aerogels develop cracks and reduce filtration efficiency at high silica content due to brittle nature of silica particle networks.

Gold nanoparticle capture within protein crystal scaffolds.

DNA assemblies have been used to organize inorganic nanoparticles into 3D arrays, with emergent properties arising as a result of nanoparticle spacing and geometry. We report here the use of engineered protein crystals as an alternative approach to biologically mediated assembly of inorganic nanoparticles. The protein crystal's 13 nm diameter pores result in an 80% solvent content and display hexahistidine sequences on their interior. The hexahistidine sequence captures Au25(glutathione)∼17 (nitrilotriacetic acid)∼1 nanoclusters throughout a chemically crosslinked crystal via the coordination of Ni(ii) to both the cluster and the protein. Nanoparticle loading was validated by confocal microscopy and elemental analysis. The nanoparticles may be released from the crystal by exposure to EDTA, which chelates the Ni(ii) and breaks the specific protein/nanoparticle interaction. The integrity of the protein crystals after crosslinking and nanoparticle capture was confirmed by single crystal X-ray crystallography.

Microfluidic devices with templated regular macroporous structures for HIV viral capture.

There is a need to develop inexpensive, portable and easy-to-use devices for viral sample processing for resource-limited settings. Here we offer a solution to efficient virus capture by incorporating macroporous materials with regular structures into microfluidic devices for affinity chromatography. Two-dimensional simulations were first conducted to investigate the effects of two structures, a nanopost array and a spherical pore network, on nanoparticle capture. Then, the two structures were created in polymers by templating anodic aluminum oxide films and 3D close-packed silica particles, respectively. When the microdevices containing functionalized porous materials were tested for human immunodeficiency virus (HIV) isolation, capture efficiencies of 80-99% were achieved under a continuous flow. Comparatively, functionalized flatbed microchannels captured around 10% of HIV particles. As the characteristic dimensions of the nanostructures are tunable, such devices can be adapted for the capture of different submicron bioparticles. The high capture efficiency and easy-to-operate nature suit the needs of resource-limited settings and may find applications in point-of-care diagnostics.