Captive Environment Research Articles

Anthropogenic effects on body size and growth in lab-reared and free-ranging Macaca mulatta.

The impact of anthropogenic pressures upon primates is increasingly prevalent, and yet the phenotypic aspects of these impacts remain understudied. Captive environments can pose unique pressures based on factors like physical activity levels and caloric availability; thus, maturation patterns should vary under differing captive conditions. Here, we evaluate the development and growth of two Macaca mulatta populations (N = 510) with known chronological ages between 9 months and 16 years, under different levels of captive management, to assess the impact of varying anthropogenic environments on primates. To track growth, we scored 13 epiphyseal fusion locales across long bones in a skeletal sample of lab-reared M. mulatta (n = 111), including the right tibia, femur, humerus, ulna, and radius. We employed a three-tier scoring system, consisting of "0" (unfused to diaphysis), "1" (fusing), and "2" (fused). To record body size, we collected five linear measures of these long bones, from the proximal and distal ends, and total lengths. Means and standard deviations were generated to compare samples; t-tests were used to determine significant differences between means. These values were compared to available data on the free-ranging, provisioned M. mulatta population of Cayo Santiago. The free-ranging monkeys (n = 274) were found to exhibit larger linear skeletal lengths (p < 0.05) than lab-reared specimens. Generally, the free-ranging macaques reached fusion at earlier chronological ages and exhibited an extended duration of the fusing growth stage. These observations may reflect the protein-rich diet provided to free-ranging monkeys and conversely, restricted movement and relaxed natural selection experienced by lab-reared monkeys.

Multiple phenotypic traits predict male mating success in a critically endangered frog

Complex sexual signals spanning multiple sensory modalities may be common in nature, yet few studies have explored how combinations of phenotypic traits influence male attractiveness and mating success. Here, we investigate whether combinations of multiple male phenotypic traits (both within and across sensory modalities) predict male mating and fertilization success in the critically endangered southern corroboree frog, Pseudophryne corroboree. We conducted breeding trials in a standardized captive environment where females were given the opportunity to choose between multiple males over the duration of the breeding season. For each male, we measured multiple call traits, aspects of coloration, body size, and age. We found that complex interactions between multiple traits best predicted male mating and fertilization success. In general, males with lower call frequency, lower call rate, and shorter call duration had the highest mating and fertilization success. Fertilization success was additionally linked to male body size and age. These findings suggest that female P. corroboree select mates based on a suite of acoustic traits, adding to a growing body of evidence that females use multiple traits to assess male quality. Our results also suggest that females may combine information from multiple signals non-additively. Moreover, our results imply that females gain direct fertility benefits from their mate choice decisions. We argue that understanding female mate choice based on various signals across multiple sensory modalities has important implications for the integration of mate choice into conservation breeding programs and needs to be considered when developing behavior-based captive breeding strategies.Significance statementSexual signals are often highly complex, yet we know little about how multiple signal components both within and across various sensory modalities predict male mating success. We investigated whether combinations of multiple phenotypic traits (within and across sensory modalities) predicted male breeding success in threatened corroboree frogs. We conducted captive breeding trials in a homogeneous environment, where females could choose between multiple males over the duration of a single breeding season. We found that interactions between multiple male traits predicted mating and fertilization success. Males with lower call frequency, call rate, and duration had higher mating success. Fertilization success was also linked to acoustic signals, body size, and age. Understanding mate choice for multiple traits further elucidates the complexity of female mate choice. This study is one of the first to consider the conservation implications of multimodal signaling in mate choice.

Avian Malaria in Penguins: Diagnostics and Future Direction in the Context of Climate Change.

Avian malaria is caused by infection with haemoprotozoa of the genus Plasmodium. Infection is endemic in large parts of the world and is typically subclinical in birds that are native to these regions. Several penguin species have evolved in non-endemic regions without the selective pressure that these parasites exert and are highly susceptible to infection when transplanted to endemic regions, for example, in the context of zoological collections or rehabilitation centers. Avian malaria in penguins typically causes acute mortality without premonitory signs, or less commonly, nonspecific signs of morbidity, followed by mortality. Additionally, infection is reported in wild penguins, though the significance of these infections remains equivocal. As global temperatures continue to increase, avian malaria is likely to pose a continued and further threat to conservation efforts in captive environments. Intra vitam diagnosis currently relies on the evaluation of blood smears and molecular methods. The former is unreliable in penguins, as the acute clinical course typically does not allow the development of parasitemia. This absence of parasitemia also makes speciation challenging. Current molecular methods typically target the Cytochrome B or 18s subunit and have proven variably sensitive and specific. Reliable intra vitam diagnosis of avian malaria and further information about the causative agents at a species level would be very valuable in understanding the epidemiology and likely future course of avian malaria infection in penguins, and in particular, the implications avian malaria may have for conservation efforts. This paper provides an overview of malaria in penguins, discusses its changing impact on management and conservation, offers a summary of current diagnostics, and suggests future direction for the development of diagnostic tests. The latter will be key in understanding and managing this disease.

Rock Installation Reduces Aggressive Behavior in Captive Hawksbill Turtles

Captive breeding programs are utilized worldwide for the recovery and conservation of endangered species, including the critically-endangered hawksbill turtle (Eretmochelys imbricata). However, aggression is an issue that is commonly reported among captive hawksbill turtles housed together. In the present study, we installed rocks in a captive environment to assess whether they can suppress aggressive behavior in captive hawksbill turtles. The behavior of immature female hawksbill turtles (n=6) was recorded over a 10 d period (between 0900 and 1700 h daily) in an experimental tank under the following conditions: (1) housed alone or paired with another individual in the (2) presence and (3) absence of rocks. Resting behavior was recorded as the total amount of time spent resting per day, while hiding and biting behaviors (i.e., aggressive behavior) were recorded as the total number of events observed per day. Serum corticosterone concentrations were measured at 0900 and 1700 h daily throughout the experimental period. The median number of hiding and biting events was significantly lower for paired individuals housed in the presence of rocks than that for those housed in the absence of rocks. Although no significant differences in serum corticosterone concentrations were observed among the experimental groups, paired individuals spent significantly more time resting in the presence of rocks. Our results suggest that underwater rock installations effectively reduce aggression in captive hawksbill turtles.

Physiological effects of capture and short-term captivity in an invasive snake species, the Burmese python (Python bivittatus) in Florida

It is important to evaluate the role of captivity as a potential stressor. An understanding of stress responses to capture and transition to captivity may inform the limitations of laboratory studies on wild animals, aid in understanding the consequences of introducing animals into captive environments, and help predict which species may be successful invasives. We investigated physiological effects of captivity by comparing at-capture blood variables in wild Burmese pythons (Python bivittatus) in Florida to pythons recently brought into captivity (1–109 days). We conducted an acute restraint test by collecting samples at baseline (immediately at handling) and one hour post-restraint across wild field-sampled (n = 19) and recently-captive (n = 33) pythons to evaluate fluctuations in plasma corticosterone, bacterial killing ability, antibody response, leukogram, and serpentovirus infection. We observed higher baseline plasma corticosterone and monocytes in recently captive compared to wild snakes, which both subsided in snakes held for a longer time in captivity, and a mild decrease in lymphocytes in the middle of the captivity period. Functional immunity and viral infection were not affected by captivity, and pythons maintained restraint-induced responses in corticosterone, heterophil to lymphocyte ratio, and monocyte counts throughout captivity. Prevalence for serpentovirus was 50%, though infection status was related to sampling date rather than captivity, indicating that viral infection may be seasonal. The history of Burmese python as a common captive animal for research and pet trade, as well as its general resilience to effects of capture and short-term captivity, may contribute to its invasion success in Florida.

Effects of Wolf Spiders' Captive Environment on Their Locomotor and Exploratory Behaviours.

Simple SummarySince the 1960s, abuses of domestic and of wild animals that have been tamed or are held in captivity have been legally prohibited and laws ensure their well-being. Many scientific investigations carried out in this context recommend ways to adapt farming and thus to avoid physical and/or psychological suffering. Evaluations of animals’ welfare in captivity entail the need to understand in detail the fundamental behaviours of the focus species and to know the degree of their variation to be able to establish objective bases that can ensure breeding conditions that respect the animals’ welfare. Current laws do not apply to invertebrate animals (such as insects or spiders) and consideration of the welfare of these animals in captivity is neglected. Here, I compared the behaviour of wild adult spiders just after collection and that of adult spiders hatched and bred in the laboratory. My results show that captivity induced rapid changes of wild spiders’ behaviour once in captivity. Therefore, it is important to establish the best breeding conditions for the needs of both invertebrate and vertebrate animals in order to promote their well-being.Here I detail the effects of the abiotic/captive environment of an adult wandering spider, Pardosa saltans (Lycosidae) on its behaviour. These studies focused on spiders collected as adults in their natural environment and spiders developed in the laboratory under controlled conditions. Wild-caught spiders were tested either immediately after capture or after being housed for 15 days post-collection. Laboratory reared spiders were kept in different environments: small or large space combined with the presence or absence of litter. Two tests evaluated by sex show the influence of these rearing conditions: an open-field test and a radial-arm maze test. The results show that wild caught spiders of both sexes tested immediately after capture weighed significantly less and were significantly more active than spiders housed in the laboratory for 15 days and spiders reared in the laboratory. Laboratory conditions induced a positive impact on body mass and negative impact on behaviour activities. The locomotor and exploratory activities of spiders of both sexes kept in container without substrate showed lower. My results suggest that the physical enrichment of the environment can reduce these negative effects for females, but not for males that seem to be more affected by being reared under controlled conditions.

Characterization of captive and wild 13-lined ground squirrel cecal microbiotas using Illumina-based sequencing

BackgroundHibernating animals experience extreme changes in diet that make them useful systems for understanding host-microbial symbioses. However, most of our current knowledge about the hibernator gut microbiota is derived from studies using captive animals. Given that there are substantial differences between captive and wild environments, conclusions drawn from studies with captive hibernators may not reflect the gut microbiota’s role in the physiology of wild animals. To address this, we used Illumina-based sequencing of the 16S rRNA gene to compare the bacterial cecal microbiotas of captive and wild 13-lined ground squirrels (TLGS) in the summer. As the first study to use Illumina-based technology to compare the microbiotas of an obligate rodent hibernator across the year, we also reported changes in captive TLGS microbiotas in summer, winter, and spring.ResultsWild TLGS microbiotas had greater richness and phylogenetic diversity with less variation in beta diversity when compared to captive microbiotas. Taxa identified as core operational taxonomic units (OTUs) and found to significantly contribute to differences in beta diversity were primarily in the families Lachnospiraceae and Ruminococcaceae. Captive TLGS microbiotas shared phyla and core OTUs across the year, but active season (summer and spring) microbiotas had different alpha and beta diversities than winter season microbiotas.ConclusionsThis is the first study to compare the microbiotas of captive and wild rodent hibernators. Our findings suggest that data from captive and wild ground squirrels should be interpreted separately due to their distinct microbiotas. Additionally, as the first study to compare seasonal microbiotas of obligate rodent hibernators using Illumina-based 16S rRNA sequencing, we reported changes in captive TLGS microbiotas that are consistent with previous work. Taken together, this study provides foundational information for improving the reproducibility and experimental design of future hibernation microbiota studies.

Hatchery and wild larval lake sturgeon experience effects of captivity on stress reactivity, behavior and predation risk.

Reintroduction programs are important tools for wildlife conservation. However, captive rearing environments may lead to maladaptive behavior and physiological alterations that reduce survival probability after release. For captive rearing programs that raise individuals captured from the wild during early ontogeny for later release, there is a lack of information about when during ontogeny the detrimental effects of captive rearing may become evident. In this study we compared cortisol levels, predation rates and swimming behavior between hatchery-produced and wild-caught larval lake sturgeon (Acipenser fulvescens), a threatened fish species, at three times over 9 days. Cortisol levels did not indicate that hatchery-produced individuals were more stressed, but cortisol reactivity to an acute stressor disappeared for both hatchery-produced and wild-caught larvae after 9 days in the hatchery. Swimming activity levels decreased over time for hatchery-produced larvae but increased over time for wild-caught larvae, suggesting that behavioral trajectories may be programmed prior to the larval stage. Neither increasing nor decreasing activity levels was advantageous for survival, as predation rates increased over time in captivity for larvae from both treatments. Results suggest that physiological and behavioral phenotypes may not accurately predict survival for individuals released from reintroduction programs and that the captive environment may inhibit transition to the wild even if cortisol levels do not indicate high stress. Findings emphasize that even a short amount of time in captivity during early ontogeny can affect phenotypes of individuals captured from wild populations, which may impact the success of reintroduction programs.

Comparative analysis of brain in relation to the body length and weight of common carp (Cyprinus carpio) in captive (hatchery) and wild (river system) populations.

Cyprinus carpio is the member of family cyprinidae commonly called common carp. This study was aimed to find out the comparison of brain of wild (river system) and captive (hatchery reared) population of common carp. A total of thirty samples (15 from hatchery and 15 from river Swat) were collected. All the specimens were examined in Laboratory of Parasitoloy, Zoology Department, University of Malakand. Findings indicated that wild population were greater in brain size and weight as compared to hatchery reared population. The fish samples collected from captive environment (hatchery) were showing more weight and length as compared to wild population of common carps. The mean value of total weight of hatchery fishes 345±48.68 and the mean value of brain weight of hatchery reared fishes 0.28±0.047. The mean value of wild fish's total body weight 195.16±52.58 and the mean value of brain weight of wild fishes are 0.45±0.14. Present research calls for the fact that fish in dependent environmental conditions possess brain larger in size as compared to its captive population, it is due to use and disuse of brain in their environmental requirements.

External Morphological and Molecular Evidence of Natural Intrageneric Hybridization between Common and Indo-Pacific Bottlenose Dolphins (Tursiops truncatus × T. aduncus) from Japanese Waters

Hybrid cetaceans occur in both captive and natural environments. This study is the first to describe the external morphological and genetic characterizations of natural intrageneric hybridization between the common bottlenose dolphin, Tursiops truncatus, and the Indo-Pacific bottlenose dolphin, T. aduncus, in Japanese waters. In November 2019, a stray dolphin was seen in Moriura Bay, Wakayama, Japan, and was a male that measured 241.5 cm in body length in July 2020, after an incidental catch in February 2020. Moderate speckles in the genital area of the dolphin were observed, which is a characteristic of T. aduncus. The dolphin had 22–23 pairs of teeth in each jaw, a quantity consistent for both species. The length of the rostrum and the size of flippers were within the range of T. aduncus. The mitochondrial DNA D-loop region haplotype was 99% homology with those from T. aduncus found in Chinese and Japanese waters. Microsatellite analysis revealed that the dolphin contained ratios of approximately 50% of both T. truncatus and T. aduncus lineages; the dolphin could therefore be an F1 hybrid between a female T. aduncus and a male T. truncatus. Further genetic studies are required to elucidate the origin of this hybrid dolphin.

Influence of environmental enrichment on the rehabilitation of white-eyed parakeet (Psittacara leucophthalmus)

The use of sensory, physical, cognitive, and alimentary stimuli are varieties of environmental enrichment used to minimize stress caused by the monotonous captive environment. The objective of this study was to verify the impact of environmental enrichment in escape-related behavioral stereotypies. Thirty birds of the species Psittacara leucophthalmus were observed from March to September 2016, received at the Wild Animal Recovery and Screening Center of the São Paulo State University (UNESP), School of the Veterinary Medicine campus Araçatuba. The methodology used for the behavioral observations was the focal animal with observation through filming were made by 18 hours per bird for three consecutive days, while physical, cognitive, food, and sensory enrichment methods were applied, and the assessment was carried out before, during, and after the application of environmental enrichment. The data were analyzed by the Shapiro-Wilk test for normality and by the Friedman test, which showed no significant difference (p &gt;0.05) before, during, and after environmental enrichment. Despite the statistical analyses, the perception of the bird’s welfare improving was visually clear.

GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS

Specimens of cultured zebrafish acquired from different fish farms in Brazil may show genetic variability and alteration in allele frequency due to genetic drift and selective pressure in a captive environment, resulting in the differentiation of productive and reproductive characteristics. The aim of this study was to evaluate the genetic variability and reproductive characteristics of 180 zebrafish specimens from six Brazilian fish farms. A deviation from the Hardy-Weinberg equilibrium was observed in all evaluated stocks. Differentiation among stocks was observed in the amount of genetic variability with respect to observed heterozygosity and the inbreeding coefficient (FIS). Genetic distance between stocks was determined through the Fst index, and the formation of four distinct groups was observed by plotting the dendrogram based on Nei’s genetic distance. Differences were observed among reproductive parameters, such as the average number of eggs per female and hatchability. This second parameter proved to be related to the level of inbreeding of the population, whereas this effect was not observed for spawning frequency. We conclude that zebrafish stocks from the 6 different Brazilian fish farms present significant genetic and phenotypic variability. The genetic structure affects fecundity and should be considered when carrying out work where reproductive rates are evaluated.

Investigating the Effect of Disturbance on Prey Consumption in Captive Congo Caecilians Herpele squalostoma

Maintaining Gymnophiona in captivity provides opportunities to study the behaviour and life-history of this poorly known Order, and to investigate and provide species-appropriate welfare guidelines, which are currently lacking. This study focuses on the terrestrial caecilian Herpele squalostoma to investigate its sensitivity to disturbances associated with routine husbandry needed for monitoring and maintaining adequate wellbeing in captivity. Fossorial caecilians gradually pollute their environment in captivity with waste products, and substrate must be replaced at intervals; doing so disturbs the animals directly and via destruction of burrow networks. As inappetence is frequently associated with stress in amphibians, the percentage consumption of offered food types, river shrimp (Palaemon varians) and brown crickets (Gryllus assimilis), was measured as an indicator of putative stress following three routine substrate changes up to 297 days post-substrate change. Mean daily variation in substrate temperatures were also recorded in order to account for environmental influences on food consumption, along with nitrogenous waste in tank substrate prior to a substrate change and fresh top soil in order to understand the trade-off between dealing with waste accumulation and disturbing animals. We found a significant negative effect of substrate disturbance on food intake, but no significant effect of prey type. Variations in daily soil temperatures did not have a significant effect on food intake, but mean substrate temperature did. Additionally, substrate nitrogenous waste testing indicated little difference between fresh and tank substrate. In conclusion, this study provides a basis from which to develop further welfare assessment for this and other rarely kept and rarely observed terrestrial caecilian species.

Captivity Influences the Gut Microbiome of Rhinopithecus roxellana.

Ex situ (captivity in zoos) is regarded as an important form of conservation for endangered animals. Many studies have compared differences in the gut microbiome between captive and wild animals, but few have explained those differences at the functional level due to the limited amount of 16S rRNA data. Here, we compared the gut microbiome of captive and wild Rhinopithecus roxellana, whose high degree of dietary specificity makes it a good subject to observe the effects of the captive environment on their gut microbiome, by performing a metagenome-wide association study (MWAS). The Chao1 index was significantly higher in the captive R. roxellana cohort than in the wild cohort, and the Shannon index of captive R. roxellana was higher than that of the wild cohort but the difference was not significant. A significantly increased ratio of Prevotella/Bacteroides, which revealed an increased ability to digest simple carbohydrates, was found in the captive cohort. A significant decrease in the abundance of Firmicutes and enrichment of genes related to the pentose phosphate pathway were noted in the captive cohort, indicating a decreased ability of captive monkeys to digest fiber. Additionally, genes required for glutamate biosynthesis were also significantly more abundant in the captive cohort than in the wild cohort. These changes in the gut microbiome correspond to changes in the composition of the diet in captive animals, which has more simple carbohydrates and less crude fiber and protein than the diet of the wild animals. In addition, more unique bacteria in captive R. roxellana were involved in antibiotic resistance (Acinetobacter) and diarrhea (Desulfovibrio piger), and in the prevention of diarrhea (Phascolarctobacterium succinatutens) caused by Clostridioides difficile. Accordingly, our data reveal the cause-and-effect relationships between changes in the exact dietary composition and changes in the gut microbiome on both the structural and functional levels by comparing of captive and wild R. roxellana.

Exploring the social network of European roe deer (Capreolus capreolus) in captivity

Socially flexible species might be at an advantage when facing environmental unpredictability, human-induced rapid environmental changes, or unnatural conditions such as encountered in captivity. The European roe deer (Capreolus capreolus) was originally described as solitarily living forest-dwelling species. In recent decades, it has expanded its range into urban and agricultural areas forming large aggregations in open habitats. Captive environments are thought to mimic some challenges encountered in rapidly changing habitats, however, to date no study has assessed how roe deer social structure changes in captive conditions. In this study, we explored the social network of a small group of captive and unrelated roe deer over the course of a ten-month period using camera traps. We found that the roe deer established a temporally stable and non-random social network with the buck as the most central and dominant individual. In addition, we analysed affiliative interactions, which have not been described in roe deer yet. We found that the affiliation network consisted not only of preferential associations between the buck and other females, but also between females, whereby a young female played a central role. The seasonal changes in roe deer’ gregariousness observed in the wild were also observed in the captive population with an increase in association strength and social interactions in autumn. These results suggest that roe deer kept in a group setting in captivity seem to flexibly adjust their social behaviour; thus, supporting the assumption that roe deer show a high social flexibility that facilitates adaptations to various habitats.

More Challenging Diets Sustain Feeding Performance: Applications Toward the Captive Rearing of Wildlife

SynopsisThe rescue and rehabilitation of young fauna is of substantial importance to conservation. However, it has been suggested that incongruous diets offered in captive environments may alter craniofacial morphology and hinder the success of reintroduced animals. Despite these claims, to what extent dietary variation throughout ontogeny impacts intrapopulation cranial biomechanics has not yet been tested. Here, finite element models were generated from the adult crania of 40 rats (n = 10 per group) that were reared on 4 different diet regimes and stress magnitudes compared during incisor bite simulations. The diets consisted of (1) exclusively hard pellets from weaning, (2) exclusively soft ground pellet meal from weaning, (3) a juvenile switch from pellets to meal, and (4) a juvenile switch from meal to pellets. We hypothesized that a diet of exclusively soft meal would result in the weakest adult skulls, represented by significantly greater stress magnitudes at the muzzle, palate, and zygomatic arch. Our hypothesis was supported at the muzzle and palate, indicating that a diet limited to soft food inhibits bone deposition throughout ontogeny. This finding presents a strong case for a more variable and challenging diet during development. However, rather than the “soft” diet group resulting in the weakest zygomatic arch as predicted, this region instead showed the highest stress among rats that switched as juveniles from hard pellets to soft meal. We attribute this to a potential reduction in number and activity of osteoblasts, as demonstrated in studies of sudden and prolonged disuse of bone. A shift to softer foods in captivity, during rehabilitation after injury in the wild for example, can therefore be detrimental to healthy development of the skull in some growing animals, potentially increasing the risk of injury and impacting the ability to access full ranges of wild foods upon release. We suggest captive diet plans consider not just nutritional requirements but also food mechanical properties when rearing wildlife to adulthood for reintroduction.

Social dominance has limited effects on spatial cognition in a wild food-caching bird.

Social dominance has long been used as a model to investigate social stress. However, many studies using such comparisons have been performed in captive environments. These environments may produce unnaturally high antagonistic interactions, exaggerating the stress of social subordination and any associated adverse consequences. One such adverse effect concerns impaired cognitive ability, often thought to be associated with social subordination. Here, we tested whether social dominance rank is associated with differences in spatial learning and memory, and in reversal spatial learning (flexibility) abilities in wild food-caching mountain chickadees at different montane elevations. Higher dominance rank was associated with higher spatial cognitive flexibility in harsh environments at higher elevations, but not at lower, milder elevations. By contrast, there were no consistent differences in spatial learning and memory ability associated with dominance rank. Our results suggest that spatial learning and memory ability in specialized food-caching species is a stable trait resilient to social influences. Spatial cognitive flexibility, on the other hand, appears to be more sensitive to environmental influences, including social dominance. These findings contradict those from laboratory studies and suggest that it is critical to investigate the biological consequences of social dominance under natural conditions.

Long‐term captivity is associated with changes to sensory organ morphology in a critically endangered insect

Captive breeding programmes are key to many threatened species reintroduction strategies but could potentially be associated with adaptations to captivity that are maladaptive in their natural habitat. Despite the importance of sensory ecology to biological fitness, few studies explore sensory system adaptations to captivity. Captive environments are devoid of predators and provide ready access to food sources and potential mates, thus reducing the need for individuals to use signals and cues to identify and locate resources or detect potential threats. With reduced complexity of the signalling environment, relaxation of selective pressures may favour reduced investment in sensory organs in captivity. We test this prediction in an iconic critically endangered invertebrate, the Lord Howe Island stick insect Dryococelus australis, which was extirpated from the island in the 1920s/30s and rediscovered on a nearby volcanic stack, Ball's Pyramid, in 2001. Using historical specimens from these populations and specimens from the 8–10th and 14th generations of a long-term conservation captive breeding programme, we examine differences in behaviourally relevant morphological traits of the compound eyes (visual organs) and antennae (olfactory organs). We find that captivity is associated with smaller compound eye size, smaller eye ommatidia and reduced density of antennal odour receptors. These morphological changes are indicative of reduced sensitivity to visual and olfactory signals and cues, and therefore are likely to have fitness implications when reintroducing a captive population into the wild. Synthesis and applications. We observe differences in sensory organ morphology between wild and captive-bred populations of the critically endangered Lord Howe Island stick insect. Our results emphasise the importance of incorporating evolutionary biology and sensory ecology into conservation programme design: to minimise the potential for captive breeding environments to compromise sensory systems that support appropriate behaviours upon reintroduction of populations into a natural habitat.

Costs and benefits of living in a vegetated, compared with non-vegetated, enclosure in male Japanese macaques (Macaca fuscata).

Improving captive environments for primates has been an important tool to enhance animal welfare. One method has been to provide primates with naturalistic vegetated enclosures to promote species-specific behaviors, enhancing interactions with their social and natural environment, such as an increase in feeding, foraging, and positive social behaviors such as play. To investigate the benefits in which living in naturalistic environments promote general animal well-being, we observed immature and adult males living in two outdoor housed groups of Japanese macaques (Macaca fuscata) in the Kyoto University Primate Research Institute (KUPRI), Inuyama, Japan, from June of 2015 until June 2016. One enclosure was naturally vegetated and the other was not. We recorded male activity budgets, affiliative (groom, play) behaviors, and rates of agonistic interactions. To examine health status, we recorded and compared coat conditions for both groups. We found that males in the vegetated enclosure spent more time in social play than males in the non-vegetated enclosure, while males in the non-vegetated enclosure displayed more stereotypic behaviors and agonistic interactions. We recorded better coat conditions in the vegetated enclosure males while rates of social grooming or self-grooming were no different between males in the two enclosures. The males in the vegetated enclosure did not have activity budgets more similar to their wild counterparts; but they spent more time in feeding-related activities and less time resting, which was more similar to their wild counterparts than males in the non-vegetated enclosure. Our findings suggest that individuals housed in naturalistic environments have significantly greater behavioral and physical markers of wellbeing than those housed in unnatural, large outdoor enclosures. Although we found that males in both types of enclosures overall had similar time budgets to males in the wild, the detailed behavioral and health results suggest that the welfare benefits to males were greater in the vegetated enclosure, compared with non-vegetated enclosures. Efforts to mimic more natural environments should promote the well-being of primates.

Domestication Strategies of Tinfoil Barb Barbonymus schwanenfeldii (Bleeker, 1854): Potential Candidate for Freshwater Aquaculture Development

This paper discussed domestication strategies of Tinfoil barb from different populations for evaluation of potential fish candidate for freshwater aquaculture development. Collection and maintenance of wild-caught adult fish was the first step in domestication strategies followed by characterization of fish, broodstocks selection and maturation, control of fish reproduction, and larval rearing. The Tinfoil barb presented an excellent performance to adapt to the new environment. This was indicated by survival rate of the fish acclimated in the captive environment of about 69.28 ± 19.64% for five weeks. Phenotypic characterization revealed that Tinfoil barb collected from Borneo, Java, and Sumatra have different morphological characters. Based on genetic characters, polymorphism percentage and heterozygosity of the Borneo tinfoil barb population provided the highest performance followed by Java and Sumatra. The growth model of all Tinfoil barb populations was allometric negative indicating a faster increase in length than in weight. Reproductive performance presented that the fecundity of Tinfoil barb female from Borneo was the highest (2795-5099 eggs/gram) followed by Sumatra (1822-2976 eggs/gram) and Java (2210 eggs/gram). The artificial reproduction performance of broodstock from Borneo also presented more excellent performance than other population after hormonal treatment. Fertility rate and hatching rate were 94,33+0,29 % and 77,44+1,03%, respectively. Java Tinfoil barb had fertility rate 93+0,05% and hatching rate 73,67+3,15%, while it was no fertility rate and hatching rate obtained from Sumatra brood-stock group. Wild-caught adult Tinfoil barb from Borneo population presented the best performance used for potential brood-stock regarding genetic characters, reproductive performances, and response to artificial reproduction. Future studies need to be concerned with the acceleration of gonad maturation and the improvement of larval rearing technology for improvement of domestication strategies of Tinfoil barb as a potential candidate for freshwater aquaculture development.