Captive Breeding Facility Research Articles

Securing genetic integrity in freshwater pearl mussel propagation and captive breeding

Securing genetic integrity is of key importance in conservation-oriented captive breeding programs releasing juveniles into the wild. This is particularly true for species such as the endangered freshwater pearl mussel (Margaritifera margaritifera) for which a number of captive breeding facilities has been established in Europe. The core objective of this study was to compare the genetic constitution of 29 cohorts of captive-bred freshwater pearl mussels from five different breeding facilities in Austria, France, Luxembourg and Germany, with their original 14 source populations from nine major European drainages, based on microsatellite markers. Captive-bred mussels represented 11 different genetic clusters, suggesting an important contribution of the breeding stations to securing the genetic diversity of the species. In almost all cases, the cultured offspring closely resembled the genetic constitution of the source mussels as revealed from the STRUCTURE analysis and the generally high assignment of offspring to the original source populations. The majority of captive-bred cohorts had an increased inbreeding coefficient and decreased genetic variability compared to their source populations as measured by AR and HO. Highest numbers of deformed juveniles coincided with very low levels of HO < 0.05. Since erosion of genetic diversity in captive breeding was mostly evident in individual year-cohorts, long-term breeding over multiple years can minimize such effects. The systematic selection of priority populations for conservation, effective breeding strategies avoiding effects of in- and outbreeding by genetically informed selection of parent individuals, and a network of collaboration among the different breeding facilities would be very useful to increase resilience and effectiveness.

Evaluation on the Legal Trade of Tokay gecko (Lacertidae; Gekkonidae; Gekko gecko Linnaeus, 1758) in Indonesia

Tokay gecko (Gekko gecko) is a large-sized gecko from the genus of Gekko, which is most commonly found from South Asia, southern China, and Southeast Asia. In Indonesia, these species are common to inhabit human-modified habitat in Sumatera, Borneo, Java, Bali, Lesser Sundas, Sulawesi, and Moluccas. In recent years, the demand for Tokay gekko with high use-value in both national and international markets has increased, one of which is used for traditional medicine. This situation raised the concern on the decreasing of the wild population and the validity of captive breeding programs that produced a large number of individuals. Several reports had estimated millions of individuals have been exported from Indonesia either legally or illegally, however, the exact number never been reported. The purpose of this study is to evaluate the trend on the harvested Tokay gecko and its origin based on the source code information. The data were collected from government records, including specimens harvested from the wild and specimens produced from captivity during 2013 - 2018 (six years). The results showed that the legally exported specimens were sourced from wild (W) and captive breeding (C or F). The total numbers of individuals exported from the wild harvest are 97.146, and all export realization is below the wild harvest quota. However, there are the large numbers of individuals exported and declared as specimens produced from the captive breeding facilities (6.965.000 with source code F, and 1.236.000 with source code C). More importantly, the high level of specimens produced from captive breeding facilities is unlikely to match with the biological capacity of this species. Therefore, we predict that specimens labeled captive breeding were likely sourced from the wild.

Mortality Associated with Bushfire Smoke Inhalation in a Captive Population of the Smoky Mouse (Pseudomys fumeus), a Threatened Australian Rodent.

A mortality event of nine threatened smoky mice (Pseudomys fumeus) occurred in January 2020 at a captive breeding facility in southeastern Australia that was affected at the time by hazardous levels of bushfire smoke, despite being more than 20 km from the nearest fire. Pathologic and clinical observations indicated smoke inhalation was the cause of death. All animals had significant pulmonary lesions, notably pulmonary edema and congestion, and moderate amounts of dark brown to black pigmented intracellular and extracellular particles from <0.5-2.5 µm in diameter were observed in the central or hilar region of the lungs of four of six animals examined histologically. Deaths occurred between three and 30 d after exposure to smoke and, for seven animals in outdoor acclimatization enclosures, were associated with very high ambient temperature (>40 C). Similar mortalities did not occur in co-located parrots, suggesting differing species sensitivity to smoke inhalation. Our findings highlight the potential for smoke to be an underdiagnosed cause of mortality in free-ranging wildlife during bushfires and for bushfires to affect wildlife populations outside of burnt areas, including in unburnt refugia. Conservation interventions for wildlife after bushfires should consider and, where possible, mitigate the risk of animals dying due to increased respiratory demand following smoke inhalation injury.

Preliminary Behavior of Chinkara (Gazella Bennettii) under Captive Conditions with Future Conservation Strategies

Background: Study of behavioral conformations play a significant role in ex-situ conservation of ungulates particularly of deer to propagate deer farming. Due to advancement in animal hunting techniques, captive breeding is the best solution to propagate animals for sports or other traditional &amp; medicinal uses. Due to the lack of management methods, captive breeding programs have been used on trial and error basis for rearing of wild animals like Chinkara.&#x0D; Objectives: The present study was planned to investigate behavioral patterns in captivity to explore factors over reproductive success of the Chinkara and potential of this wild animal (Chinkara) for deer farming practices in Pakistan.&#x0D; Methodology: Present study was conducted on the behavior of Chinkara (Gazella bennettii) [wild-caught (WC)=20, captive-bred (CB)=10] for a period of one year from April 2013 to March 2014 at captive breeding facilities for ungulates, Ravi campus Pattoki, University of Veterinary and Animal Sciences (UVAS), Pakistan.&#x0D; Results: The behavior of captive-born and wild-caught animals was observed by focal sampling pattern to frame conservation strategies for successful management practices for promotion of deer farming in Pakistan. Similar behavioral patterns were observed in both WC and CB animals but WC male Chinkara displayed a higher degree of agonistic interaction than of CB males. From these results it is predicted that there is no obvious immediate effect of captivity on behavioral configurations up to 10 generations in Chinkara.&#x0D; Conclusion: It is suggested that Chinkara is not suitable for domestication like Goat until further studies on ethology of Chinkara.

Prion Protein Gene (PRNP) Sequences Suggest Differing Vulnerability to Chronic Wasting Disease for Florida Key Deer (Odocoileus virginianus clavium) and Columbian White-Tailed Deer (O. v. leucurus).

Chronic wasting disease (CWD) is a fatal, highly transmissible spongiform encephalopathy caused by an infectious prion protein. CWD is spreading across North American cervids. Studies of the prion protein gene (PRNP) in white-tailed deer (WTD; Odocoileus virginianus) have identified non-synonymous substitutions associated with reduced CWD frequency. Because CWD is spreading rapidly geographically, it may impact cervids of conservation concern. Here, we examined the genetic vulnerability to CWD of 2 subspecies of WTD: the endangered Florida Key deer (O. v. clavium) and the threatened Columbian WTD (O. v. leucurus). In Key deer (n = 48), we identified 3 haplotypes formed by 5 polymorphisms, of which 2 were non-synonymous. The polymorphism c.574G>A, unique to Key deer (29 of 96 chromosomes), encodes a non-synonymous substitution from valine to isoleucine at codon 192. In 91 of 96 chromosomes, Key deer carried c.286G>A (G96S), previously associated with substantially reduced susceptibility to CWD. Key deer may be less genetically susceptible to CWD than many mainland WTD populations. In Columbian WTD (n = 13), 2 haplotypes separated by one synonymous substitution (c.438C>T) were identified. All of the Columbian WTD carried alleles that in other mainland populations are associated with relatively high susceptibility to CWD. While larger sampling is needed, future management plans should consider that Columbian WTD are likely to be genetically more vulnerable to CWD than many other WTD populations. Finally, we suggest that genetic vulnerability to CWD be assessed by sequencing PRNP across other endangered cervids, both wild and in captive breeding facilities.

Cuddle, kill, conserve: a posthuman analysis of the African lion within the South African wildlife security assemblage

PurposeThis paper explores the life cycle of a captive bred lion in South Africa, focusing on the distinction between captive bred and wild individuals. Lions are bred in captive breeding facilities across the country to provide cubs and teenagers for ecotourism, and following this, hunting “trophies.” A distinction is made between the “wild” and “captive” lion, a categorization that I argue legitimizes violent and unethical treatment toward those bred specifically to be cuddled and killed. This analysis explores how the lion is remade or modified from wild to commodity and the repercussions this has had throughout the wildlife security assemblage.Design/methodology/approachThe paper is based on ethnographic research carried out in South Africa during 2016 that involved conducting informal and semi-structured interviews with activists, breeders, wildlife security personnel and conservationists drawing out the interspecies relations that influenced the encounters between humans and wildlife.FindingsDominant conservation narratives continue to understand and interpret wildlife solely as a commodity or profitable resource, which has led to the normalization of unethical and cruel practices that implicate wildlife in their own security and sustenance through their role in ecotourism, hunting and more recently, the lion bone trade. Captive bred lions are treated as products that undergo a series of translations through which they are exposed to violence and exploitation operationalized through practices linked to conservation and ecotourism.Originality/valueThrough posthuman thinking, this paper contributes to debates on the interspecies dimensions of politics through challenging the dominant assumptions that govern conservation and the interspecies encounter.

The important role of private wildlife organisations in facilitating scientific research: A case study of the Australian Dingo Foundation

As we enter an era of global mass extinctions, it is important to tackle wildlife research and conservation from multiple fronts, including those made available by wildlife organisations, zoos and sanctuaries. Captive studies are particularly useful when studying free-ranging populations is difficult, and/or when controlled conditions are required. Yet, despite the significant role that they play in supporting research and conservation of species and ecosystems, they are rarely recognised in the scientific literature. Here we present a case study of the Australian Dingo Foundation (ADF), a private organisation and captive breeding facility that actively supports research and conservation efforts relating to the dingo (Canis dingo). Over the past decade (2010 to 2020), the ADF has facilitated research across eight research disciplines that include archaeology, behaviour, biology, cognition, evolutionary psychology, non-lethal management, reproduction and parental behaviour, and vocalisations. This has resulted in at least 21 published scientific studies which are summarised in this paper. As this case study demonstrates, captive facilities have the potential to contribute to the understanding and conservation of dingoes by providing practical alternatives to, and/or supplement studies of free-ranging populations. We conclude by outlining some of the implications and limitations of conducting research using captive dingo populations.

Fear and stressing in predator-prey ecology: considering the twin stressors of predators and people on mammals.

Predators induce stress in prey and can have beneficial effects in ecosystems, but can also have negative effects on biodiversity if they are overabundant or have been introduced. The growth of human populations is, at the same time, causing degradation of natural habitats and increasing interaction rates of humans with wildlife, such that conservation management routinely considers the effects of human disturbance as tantamount to or surpassing those of predators. The need to simultaneously manage both of these threats is particularly acute in urban areas that are, increasingly, being recognized as global hotspots of wildlife activity. Pressures from altered predator–prey interactions and human activity may each initiate fear responses in prey species above those that are triggered by natural stressors in ecosystems. If fear responses are experienced by prey at elevated levels, on top of responses to multiple environmental stressors, chronic stress impacts may occur. Despite common knowledge of the negative effects of stress, however, it is rare that stress management is considered in conservation, except in intensive ex situ situations such as in captive breeding facilities or zoos. We propose that mitigation of stress impacts on wildlife is crucial for preserving biodiversity, especially as the value of habitats within urban areas increases. As such, we highlight the need for future studies to consider fear and stress in predator–prey ecology to preserve both biodiversity and ecosystem functioning, especially in areas where human disturbance occurs. We suggest, in particular, that non-invasive in situ investigations of endocrinology and ethology be partnered in conservation planning with surveys of habitat resources to incorporate and reduce the effects of fear and stress on wildlife.

Incorporating habitat suitability and demographic data for developing a reintroduction plan for the critically endangered yellow spotted mountain newt, Neurergus derjugini

In reintroduction programmes for amphibians, data on age structure in hosting populations and choices of life stage or age groups in releasing captive bred individuals are often missing. Similarly, employing site selection procedures for selecting appropriate reintroduction locations are often neglected. Here, we obtained data on longevity, age at maturation, and age structure from skeletochronological data in a free living population of the yellow spotted mountain newt, Neurergus derjugini. A maximum longevity of 13 years for males and 12 years for females showed that N. derjugini is a long living newt with a stable age structure. We also employed maximum entropy modelling, geographic information system, and multicriteria decision analysis to obtain ranked suitability scores for reintroduction sites. Finally, we determined post-release survival rates for different life stage and age groups of N. derjugini including 30 eggs and 60 individuals of six-months old larvae, one and three-year old juveniles, and six-year old adults (15 each) born and raised in a captive-breeding facility and released into mesh enclosures in a selected stream. Over 10 visits to the site before and after overwintering, the survival rates for eggs, larvae, one and three-year juveniles and six-year old adults were 25, 80, 86.66, 93.33 and 53.33 % respectively. Applying survival rates obtained from current experimental reintroductions through a static life table suggest that an optimal release strategy to arrive at a numerical target of 100 adults aged three can be achieved by reintroduction of 650 fertilised eggs and fostering them in meshed enclosures in the selected stream.

Skin bacterial microflora of two closely related mountain newts (Salamandridae) – the Yellow‐spotted mountain newt Neurergus derjugini and the Kaiser's mountain newt Neurergus kaiseri – in the wild and in a breeding facility highlight new conservation perspectives

Microbiome research is important for the identification of factors that are associated with the ex situ care of amphibians, such as potential contagious and lethal pathogens. Nevertheless, it can be also used to establish whether newts can adapt to exposure to new microbial communities, which would be important for the success of future reintroductions into the natural habitat. There is no available information regarding the skin flora naturally occurring in mountain newts of the genus Neurergus, including the Yellow‐spotted mountain newt Neurergus derjugini and the Kaiser's mountain newt Neurergus kaiseri. In this study, skin bacterial microbiota of wild adults and individuals of both species from a captive‐breeding facility (CBF) were compared. Four bacteria that naturally occur on the skin of wild adult N. derjugini were identified (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli). Six bacteria were positively detected on the skin of wild adult N. kaiseri [S. aureus, B. subtilis, B. cereus, E. coli, Rhodococcus equi, Klebsiella (Enterobacter) aerogenes]. Our results indicate that the skin microbiota of F1 offspring (≤ 2 months of age) from the CBF did not correspond exactly to the microbial community identified in wild adult N. derjugini. However, ≥ 75% of the bacteria found in older individuals (≥ 1 year of age) living at the CBF over the long term corresponded to those of their wild counterparts. It would appear that post‐metamorphic and adults of both species might be better able to resist and adapt to natural microbiota than larvae.

Psittacid Adenovirus-2 infection in the critically endangered orange-bellied parrot (Neophema chrysogastor): A key threatening process or an example of a host-adapted virus?

Psittacid Adenovirus-2 (PsAdv-2) was identified in captive orange-bellied parrots (Neophema chrysogastor) during a multifactorial cluster of mortalities at the Adelaide Zoo, South Australia, and an outbreak of Pseudomonas aeruginosa septicaemia at the Tasmanian Department of Primary Industries, Parks, Water and Environment captive breeding facility, Taroona, Tasmania. This was the first time that an adenovirus had been identified in orange-bellied parrots and is the first report of PsAdv-2 in Australia. To investigate the status of PsAdv-2 in the captive population of orange-bellied parrots, 102 healthy birds from five breeding facilities were examined for the presence of PsAdv-2 DNA in droppings and/or cloacal swabs using a nested polymerase chain reaction assay. Additionally, eight birds released to the wild for the 2016 breeding season were similarly tested when they were recaptured prior to migration to be held in captivity for the winter. PsAdv-2 was identified in all breeding facilities as well as the birds recaptured from the wild. Prevalence of shedding ranged from 29.7 to 76.5%, demonstrating that PsAdv-2 is endemic in the captive population of orange-bellied parrots and that wild parrots may have been exposed to the virus. PsAdv-2 DNA was detected in both cloacal swabs and faeces of the orange-bellied parrots, but testing both samples from the same birds suggested that testing faeces would be more sensitive than cloacal swabs. PsAdv-2 was not found in other psittacine species housed in nearby aviaries at the Adelaide Zoo. The source of the infection in the orange-bellied parrots remains undetermined. In this study, PsAdv-2 prevalence of shedding was higher in adult birds as compared to birds less than one year old. Preliminary data also suggested a correlation between adenovirus shedding prevalence within the breeding collection and chick survival.

Animal Welfare in Conservation Breeding: Applications and Challenges.

Animal welfare and conservation breeding have overlapping and compatible goals that are occasionally divergent. Efforts to improve enclosures, provide enriching experiences, and address behavioral and physical needs further the causes of animal welfare in all zoo settings. However, by mitigating stress, increasing behavioral competence, and enhancing reproduction, health, and survival, conservation breeding programs must also focus on preparing animals for release into the wild. Therefore, conservation breeding facilities must strike a balance of promoting high welfare, while minimizing the effects of captivity to increase population sustainability. As part of the Hawaii Endangered Bird Conservation Program, San Diego Zoo Global operates two captive breeding facilities that house a number of endangered Hawaiian bird species. At our facilities we aim to increase captive animal welfare through husbandry, nutrition, behavior-based enrichment, and integrated veterinary practices. These efforts help foster a captive environment that promotes the development of species-typical behaviors. By using the “Opportunities to Thrive” guiding principles, we outline an outcome-based welfare strategy, and detail some of the related management inputs, such as transitioning to parental rearing, and conducting veterinary exams remotely. Throughout we highlight our evidence-based approach for evaluating our practices, by monitoring welfare and the effectiveness of our inputs. Additionally we focus on some of the unique challenges associated with improving welfare in conservation breeding facilitates and outline concrete future steps for improving and evaluating welfare outcomes that also meet conservation goals.

Survey of Reticuloendotheliosis Virus in Wild Turkeys (Meleagris gallopavo) in Texas, USA.

Reticuloendotheliosis virus (REV) is an immunosuppressive and sometimes oncogenic avian retrovirus that establishes lifelong infection in a wide range of avian species. REV-infected wild birds roaming near at-risk captive flocks, such as is the case for the highly endangered Attwater's Prairie Chicken (APC; Tympanuchus cupido attwateri), could act as a reservoir for viral transmission. In wild birds, prevalence rates of REV are low and appearance of associated disease is uncommon. During 2016-17, nearly half of all captive adult APC mortality at Fossil Rim Wildlife Center captive breeding facility in Glen Rose, Texas, US was attributed to REV infection. The unusually high REV prevalence rate prompted us to survey for this virus in wild galliforms throughout the region. From 2016-17, 393 blood samples collected from two subspecies of Wild Turkeys (Meleagris gallopavo) were tested for REV proviral DNA through amplification of the viral 3' long terminal repeat and segments of the viral pol gene. In REV-affected counties, 5% (5/98) of native Rio Grande Wild Turkeys (Meleagris gallopavo intermedia) were identified as REV-positive. In addition, we detected REV in one of 62 Eastern Wild Turkeys (Meleagris gallopavo silvestris) that had been imported during conservation efforts. To better determine protective measures, continued surveillance, including collection and genetic analysis of REV-infected samples, is necessary to identify sources of REV outbreaks in captive APC flocks.

Physiological impacts of housing maned wolves (Chrysocyon brachyurus) with female relatives or unrelated males

The maned wolf is a threatened canid species native to South America. Previous studies have suggested the species exhibits induced ovulation. In captive breeding facilities, reproductive success is low while rates of neonatal mortality are high. Females that are not recommended for breeding are frequently housed together. However there has never been a systematic study of the reproductive consequences of co-housing females. This study was conducted for three purposes, to: (1) corroborate the presence of induced ovulation, (2) determine whether elevated cortisol is implicated in neonatal pup mortality, and (3) evaluate the endocrine correlates of group housed females. Using fecal hormone monitoring for estrogen, progesterone, and cortisol, 43 cycles from 33 female maned wolves were studied from 2002 to 2015. Females were categorized by their reproductive status: pregnant and successfully raised pups (PR; n = 11), pregnant with neonatal pup demise within 3 days (PL; n = 7), housed with a male but no signs of breeding or pregnancy (PP; n = 10), housed singly (S; n = 8), or housed with related females (F; n = 7). Estrogen and progestagen remained at baseline for all females not housed with a male (S, F), while elevations consistent with ovulation were seen in females housed with a male (PP, PL, PR). Compared to PR females, PL individuals showed similar cortisol levels throughout the cycle and slightly lower progesterone levels during gestation. As for the effect of co-housing related females, F females showed estrogen and progesterone levels lower even than S females while cortisol levels were elevated compared to all other groups. These findings support the previous evidence of induced ovulation in the maned wolf. Although elevated cortisol does not seem to be implicated in pup loss, a non-significant trend towards lower progesterone during gestation could be implicated. Future studies should assess depressed progesterone levels as a correlate to neonatal pup mortality. Female maned wolves housed with related females experience suppressed reproductive hormones and elevated adrenal hormones. Therefore, a more systematic study of hormonal and behavioral correlates to co-housing with related females is warranted.

Clinical, virological and epidemiological characterization of an outbreak of Testudinid Herpesvirus 3 in a chelonian captive breeding facility: Lessons learned and first evidence of TeHV3 vertical transmission.

Testudinid herpesviruses (TeHVs) have a worldwide distribution among tortoises. However, information such as risk factors promoting the occurrence or the recrudescence of the associated disease and the mid-term sequelae of an outbreak comprising the extent and dynamic of the viral shedding have been only minimally investigated. Critical management information is also lacking or anecdotal. Furthermore, major aspects of the viral pathogenesis including the likelihood of vertical transmission of the virus are virtually unknown. The present study describes the occurrence and the management of an outbreak of Testudinid herpesvirus genotype 3 (TeHV3) in a large, private collection of chelonians. Clinical, pathological, molecular and serological characterization of the outbreak were carried out. Seventy-five percent of the infected tortoises died. Complementation of molecular and serological testing was a critical point for successful management implementations. A case-control study was performed to analyze possible risk factors associated with the infection. Furthermore, a subgroup of six asymptomatic infected tortoises was monitored for two consecutive seasons after the outbreak: all the infected tortoises were determined to be intermittent shedders, except one, which was a persistent shedder. Post-hibernation was associated with the highest number of shedders. Finally, evidence of the most likely vertical transmission of the virus was obtained for the first time.

Sexual Behaviour of the Spectral Tarsier (Tarsius spectrum) in Captivity

Tarsius spectrum is a primate species endemic to Sulawesi. Populations of the species have decreased due to habitat destruction and hunting. The sexual behaviour of T. bancanus and T. syrichta are known, but that of T. spectrum has not been reported until recently. The aim of this research was to study the sexual behaviour of T. spectrum in captivity. We observed 3 pairs of T. spectrum at the captive breeding facility of the IPB Primate Research Centre for 9 months using focal animal sampling. We showed that principal courtship behaviours were scent marking (36.7%) and genital marking for females (16.2%) and genital inspection for males (16.0%). Copulations lasted between 3 and 4 min, starting with the male mounting the female and thrusting quickly as many as 168–236 times followed by slow thrusting 9–20 times. When slow thrusting occurred, females vocalized up to 6 times. At the end of the copulation sequence, males remained motionless with their penis inserted within the female’s genitalia for about 31 s. Copulation occurred only once for each pair during the observation period. Our results should be useful to support breeding programmes and conservation actions for tarsiers.

Aktivitas Makan Alap-Alap Capung (Microhierax fringillarius Drapiez, 1824) pada Masa Adaptasi di Kandang Penangkaran

ABSTRACT Black thighed falconet is smallest bird from falconidae. This study aimed to determine nutrient requirement and describe feeding activity of black thighed falconet (Microhierax fringillarius Drapiez, 1824) on the adaptation period at the captive breeding facility. This observation was conducted at the bird captivity of Research Center of Biology. Black tighed falconet in this research have weighted about 33.65 ± 2.48 grams. Feed given once a day in the morning consist of scaly-breasted munia (Lonchura punctulata), mice, frozen tilapia fish and frozen beef. Observation of feeding activity using a focal animal sampling technique for 20 days. Data were analyzed descriptive method. Observations started in the morning at 08:00 am until feeding activity finished. Parameter measured were feeding activity, feed intake and feeding time needed. Temperature and humidity recorded in the morning (8:00 am), noon (12:00 pm), and afternoon (4:00 pm). Based on observations, the highest feed intake was mice (3,71 g/head/day) with a gross energy of 217.23 cal/head/day and the lowest was tilapia fish (2.52 g/head/days) with a gross energy of 147.52cal/head/day. The fastest meal time needed was mice (29.47±1.54 minutes) and the slowest was tilapia fish (46.51 ± 3.51 minutes). Keyword: Falconidae, feeding behavior, captive breeding

Masked Bobwhite: Status of an Endangered Subspecies

The masked bobwhite (Colinus virginianus ridgwayi), first collected in 1884, soon disappeared and was considered extinct by 1950. Its rediscovery in 1964 precipitated an aggressive effort to restore the masked bobwhite within its range in both the United States and Mexico. The masked bobwhite, despite this effort, has continued to decline with a precipitous decrease in numbers in the last decade. Surveys conducted in 2009 and 2010 resulted in no detections; for all practical purposes the masked bobwhite is now extinct in the wild. Fortunately, a captive population continues to exist and with proper management can produce sufficient masked bobwhite to restore the wild population. The Masked Bobwhite Recovery Program has been developed and is implementing a bi-national recovery strategy with Mexican cooperators that includes: (1) construction of a new captive breeding facility on Buenos Aires National Wildlife Refuge and adoption of new husbandry and rearing protocols; (2) contracting with the San Diego Zoo Conservation Research Center to manage and operate the new facility; (3) construction of a new facility at Africam Safari, Puebla, Mexico; (4) creation of a reintroduction program in Sonora, Mexico; (5) development of a Mexican landowner outreach program; and (6) a habitat improvement and predator management program on Buenos Aires National Wildlife Refuge.

International trade in endangered species: the challenges and successes of the 17th conference of parties to the convention on international trade in endangered species of wild fauna and flora (CITES)

International trade in endangered species: the challenges and successes of the 17th conference of parties to the convention on international trade in endangered species of wild fauna and flora (CITES)

Variation in food availability mediate the impact of density on cannibalism, growth, and survival in larval yellow spotted mountain newts (Neurergus microspilotus): Implications for captive breeding programs.

In this study, we examined cannibalistic behavior, growth, metamorphosis, and survival in larval and post-metamorph endangered yellow spotted mountain newts Neurergus microspilotus hatched and reared in a captive breeding facility. We designed a 2 × 2 factorial experiment, crossing two levels of food with two levels of density including high food/high density, high food/low density, low food/high density, and low food/low density. The level of cannibalistic behavior (including the loss of fore and hind limbs, missing toes, tail, gills, body damage, and whole body consumption) changed as the larvae grew, from a low level during the first 4 weeks, peaking from weeks 7 to 12, and then dropped during weeks 14-52. Both food level and density had a significant effect on cannibalism. The highest frequency of cannibalism was recorded for larvae reared in the low food/high density and lowest in high food/low density treatments. Growth, percent of larval metamorphosed, and survival were all highest in the high food/low density and lowest in low food/high density treatment. Food level had a significant effect on growth, metamorphosis, and survival. However, the two levels of density did not influence growth and metamorphosis but showed a significant effect on survival. Similarly, combined effects of food level and density showed significant effects on growth, metamorphosis, and survival over time. Information obtained from current experiment could improve productivity of captive breeding facilities to ensure the release of adequate numbers of individuals for reintroduction programs. Zoo Biol. 35:513-521, 2016. © 2016 The Authors. Zoo Biology published by Wiley Periodicals, Inc.