Capsule Research Articles

The role of staphylococcal polysaccharide microcapsule expression in septicemia and septic arthritis.

Staphylococcus aureus arthritis is a rapidly progressive and highly erosive disease of the joints in which both host and bacterial factors are of pathogenic importance. One potential bacterial virulence factor is the ability to express a polysaccharide capsule (CP). Among 11 reported capsular serotypes, CP type 5 (CP5) and CP8 comprise 80 to 85% of all clinical blood isolates. The aim of this study was to assess the role of CP5 as a virulence factor in staphylococcal septicemia and septic arthritis with a recently established murine model of hematogenously spread S. aureus arthritis. NMRI mice were inoculated intravenously with S. aureus strains isogenic for expression of CP5, and clinical, bacteriological, serological, and histopathological progression of disease was studied. Inoculation of 7 x 10(6) CFU of S. aureus per mouse induced 55% mortality in the group inoculated with the CP-expressing bacteria, compared to 18% in the group inoculated with CP- mutants. A lower dose of inoculum (3 x 10[6] per mouse) did not give rise to mortality in mice inoculated with CP mutant strains, whereas 18% of the mice inoculated with the CP5-expressing S. aureus died. Importantly, mice inoculated with S. aureus expressing CP5 had a significantly higher frequency of arthritis and a more severe form of the disease. In vitro assays suggested that macrophages were not able to phagocytize CP5+ staphylococci as efficiently as they were CP5- strains. In addition, once phagocytized, CP5+ bacteria were less efficiently killed than CP- mutants. In summary, CP5 leads to a higher frequency of arthritis and a more severe course of the disease. This seems to be related to the effects of the downregulatory properties of CP on the ingestion and intracellular killing capacity of phagocytes. Our results clearly indicate that the expression of CP5 is a determinant of the virulence of S. aureus in arthritis and septicemia.

The thermal properties of bovine joint capsule. The basic science of laser- and radiofrequency-induced capsular shrinkage.

Orthopaedic surgeons have recently adapted the holmium: yttrium-aluminum-garnet (YAG) laser for the shrinkage of capsular tissues for treatment of glenohumeral instability. The molecular mechanism of capsular shrinkage has not been documented to date. This study examined the effects of heating on bovine calf knee capsule and subsequent shrinkage of the capsule. Capsule specimens were placed in a saline bath at temperatures ranging from 55 degrees to 75 degrees C for 1, 3, 5, and 10 minutes. Shrinkage was quantified by digital imaging, and the tissue was examined by light and polarized light microscopy. Tissue contraction was not measurable at or below 57.5 degrees C. At 60 degrees C, tissue shrinkage occurred with corresponding basophilic staining and loss of birefringence in collagen fibers. For specimens heated at 60 degrees C and 62 degrees C, shrinkage directly correlated with duration of thermal exposure. Maximal shrinkage of approximately 50% in length occurred at and above 65 degrees C with thermal exposures of 1 minute or greater. This study demonstrates that thermal shrinkage of bovine knee capsule correlates with denaturation of collagen fibers and depends on both time and temperature. Capsular shrinkage treatments may be performed with any energy source that is capable of well-controlled heating of capsular tissue and does not depend on the special properties of laser light.

Arthroscopic capsular release for the stiff shoulder. Description of technique and anatomic considerations.

The anatomic proximity of several neurovascular structures remains a major concern to the surgeon interested in performing arthroscopic capsular release. We evaluated the anatomic relationships between the released capsule and the axillary nerve, posterior circumflex humeral artery, and brachial artery in a frozen cadaveric model. With the aid of electrocautery, seven cadaveric shoulders underwent complete arthroscopic capsular release. The release was performed circumferentially, approximately 1 cm lateral to the glenoid rim. All shoulders were subsequently frozen and sectioned through the plane of the capsular release while the shoulder was maintained in the lateral arthroscopic position (45 degrees of abduction and 20 degrees of flexion). Anatomic dissection revealed an average distance from the capsular release to the axillary nerve of 7.04 mm (95% confidence interval, 5.62, 8.47), to the posterior circumflex humeral artery of 8.2 mm (95% confidence interval, 6.41, 9.99), and to the brachial artery of 15.97 mm (95% confidence interval, 9.85, 22.09). As the axillary nerve was followed medially from the released capsule, the inferior border of the subscapularis muscle became interposed between the capsule and the axillary nerve. This limited anatomic study shows that a relatively safe margin between the capsule and the neighboring neurovascular structures can be obtained by releasing the capsule within 1 cm of the glenoid rim.

Books received and capsule reviews

Books received and capsule reviews

Gel-filled temazepam capsule ban proposed

Gel-filled temazepam capsule ban proposed

Unique ability of the Proteus mirabilis capsule to enhance mineral growth in infectious urinary calculi.

Struvite (MgNH4PO4.6H2O) calculi are a common complication of Proteus mirabilis urinary tract infections. Although urease is a major virulence factor in calculus formation, the polysaccharide capsule (CPS) of this organism also enhances struvite crystallization and growth in vitro (L. Clapham, R. J. C. McLean, J. C. Nickel, J. Downey, and J. W. Costerton, J. Crystal Growth 104:475-484, 1990). We obtained purified CPS, of known structure and varying anionic character, from P. mirabilis ATCC 49565 and several other organisms. Artificial urine was added to CPS, and the pH was elevated from 5.8 to 8.5 by the addition of urease or titration with 0.25 M NH4OH to induce struvite crystallization. Crystallization was measured by particle counting (Coulter counter), and the morphology (crystal habit) was examined by phase-contrast microscopy. In the presence of partially anionic P. mirabilis CPS, struvite formation occurred at a lower pH than in the absence of CPS or in the presence of other neutral, partially anionic, or anionic CPS. At pH 7.5 to 8.0, significantly more struvite crystals formed in the presence of P. mirabilis CPS than under other experimental conditions. With the exception of one polymer (curdlan) which did not bind Mg2+, enhancement of struvite formation by CPS polymers was inversely proportional to their Mg2+ binding ability. We speculate that the structure and partial anionic nature of P. mirabilis CPS enable it to enhance struvite formation by weakly concentrating Mg2+ ions during struvite crystal formation. This illustrates a new virulence aspect of bacterial CPS during infection.

Relationships of the Cyclodorippoidea Ortmann: evidence from spermatozoal ultrastructure in the generaXeinostoma, TymolusandCymonomus(Crustacea, Decapoda)

Summary The spermatozoa of two genera and species of Cyclodorippidea, Xeinostoma richeri (Xeinostominae) and Tymolus sp. (Cyclodorippinae), and one species of Cymonomus sp. (Cymonomidae) are found to constitute a distinctive cyclodorippoid sperm-type characterized by (1) sperm anteroposteriorly depressed, mean ratio of length to width 0.54–0.63; (2) operculum extending to the lateral limits of the acrosome (autapomorphy of cyclodorippoids) and centrally perforate or (Cymonomus sp.) thinner; (3) contents of acrosome vesicle with two major horizontal zones, as in homolids and dynomenids, including a dense lower (posterior) zone; (4) perforatorium very wide (0.3 width of acrosome), anteriorly rounded, not capitate, lacking radiate projections; (5) acrosomal capsule with external projections over its posterior half; (6) slender dense filaments extending into the perforatorium from its walls, their bases associated with corrugations of its basal wall; (7) nucleus, cupping the acrosome and cytoplasm, with well-...

Intracapsular Subtotal Prostatectomy in Normal Dogs: Use of an Ultrasonic Surgical Aspirator

In an attempt to reduce postoperative incontinence associated with excisional prostatectomy and the recurrence of prostatic disease after less radical surgeries, a new technique for subtotal prostatectomy was developed using an ultrasonic surgical aspirator. During laparotomy, a longitudinal incision was made in the ventral surface of each lobe of the prostate gland. Biopsies for histology and culture were taken. Through the ventral incisions, the ultrasonic surgical aspirator was used to resect approximately 85% of the glandular tissue from inside the capsule. The ultrasonic surgical aspirator allowed resection of the glandular tissue without damaging the vessels, nerves, and connective tissue stroma. The ventral portion of the capsule was excised. The dorsal portions of the capsule were apposed on the ventral midline by suturing their ventral margins to form a periurethral cuff. As preclinical research, seven intact male dogs were evaluated by physical examination, complete blood cell count, serum urea nitrogen, urinalyses, and urethral pressure profile (UPP) with urethral electromyograms (EMGs). The dogs underwent subtotal prostatectomy and castration. All dogs were continent after surgery. UPPs and EMGs at 1 and 2 months after surgery were no different than before surgery. This contrasts to our previous studies in which excisional prostatectomy reduced maximal urethral closure pressure (MUCP) in dogs without prostatic disease. The use of the ultrasonic surgical aspirator for intracapsular subtotal prostatectomy facilitated removal of glandular tissue with decreased hemorrhage and provided preservation of the prostatic urethra, nerves, and vessels involved in maintaining urinary continence.

A Pseudolipoma on the Diaphragmatic Surface of the Liver in a Female B6C3F1 Mouse

A Pseudolipoma on the Diaphragmatic Surface of the Liver in a Female B6C3F1 Mouse

Study on the Function of Polamine Capsule Soaked in P204

Study on the Function of Polamine Capsule Soaked in P204

Occurrence of a capsule inAeromonas salmonicida

Occurrence of a capsule in<i>Aeromonas salmonicida</i>

Update for tablet and capsule guide

Update for tablet and capsule guide

SELECTIONS, INTENTIONS, INTUITIONS, AND CHAOS: SOME CAPSULE REVIEWS

SELECTIONS, INTENTIONS, INTUITIONS, AND CHAOS: SOME CAPSULE REVIEWS

Comparison of the subrenal capsule assay and succinate dehydrogenase inhibition test as drug sensitivity tests for cancer.

The same chemotherapeutic agents were tested against fresh surgical explants of solid tumors obtained from 50 patients using the in vivo subrenal capsule (SRC) assay and the in vitro succinate dehydrogenase inhibition (SDI) test in comparison. Control growth adequate to meet evaluable assay criteria was obtained in 36 of the 50 tumors tested in the SRC assay (72.0%). In the SDI test, 46 of 50 tumors were evaluable (92.0%). Correlations between the two test systems were dependent upon the activity criteria established for each system. With activity criteria set at a change of less than or equal to -2.0 in the drug sensitivity score for the SRC assay and greater than or equal to 50.0% inhibition of succinate dehydrogenase activity for the SDI test, 12.5% of the drugs tested were active in the SRC assay and 22.3% were active in the SDI test. Correlations of tumor response between the two test systems were 31.7% for sensitivity (13/41) and 95.1% for resistance (98/103). In spite of the fundamental difference between the SRC assay and SDI test, meaningful correlations between the test results and clinical tumor responses in both test systems were obtained. This fact suggests that the two methods are complementary to each other.

1986 annual meeting capsule

1986 annual meeting capsule

Localization of O9-lipopolysaccharide within the Escherichia coli K29 capsule

Localization of O9-lipopolysaccharide within the Escherichia coli K29 capsule

Closure to “ Lift and Drag on Stationary Capsule in Pipeline ” by Henry Liu and Harald R. Graze (January, 1983)

Closure to “ <i>Lift and Drag on Stationary Capsule in Pipeline</i> ” by Henry Liu and Harald R. Graze (January, 1983)

Try it in Capsule from

Try it in Capsule from

The Menu as Culture Capsule

The Menu as Culture Capsule

A study on motility of Rest-Stomach after gastrectomy by Telemetering Radio Capsule

A study on motility of Rest-Stomach after gastrectomy by Telemetering Radio Capsule