The study was conducted to investigate the effect of adding different concentrations of plant growth regulators of Benzyl adenine BA, KIN and 2 iP to MS nutritional medium on the growth of single nodes of Capparis spinosa plant. The results showed that in plant length factor the treatments of 2 and 1 mg / liter 2iP were significantly higher compared with the other treatments and the obtained plants reached average length of 3,3.3,1 cm respectively in these treatments, while for the average number of branches and leaves factors, the treatment 2 mg / liter of BA was significantly higher compared with other treatments, the produced plants arrive to 3.6 branches and 14.1 leaves. For rooting system, the treatment of MS supplemented with IBA growth regulator with a concentration of 2 mg / liter was significantly higher for both length and number of roots. The obtained plants were adapted using different treatments but the treatment of mixture peat moss and sand 1: 2 was the best