The co-infection of ehrlichiosis and anaplasmosis is a common condition in small animal clinics, mainly transmitted by the tick Rhipicephalus sanguineus, which can cause several serious clinical signs, including progression to death. A routine check was performed on a female dog of the Lhasa Apso breed, five years old, weighing 5.6 kg, resident to the municipality of Salto, in the state of São Paulo (Brazil). During anamnesis, the tutor reported that the animal was previously treated for a hemoparasitosis, recovering from clinical signs for more than 24 months, taking Afoxolaner periodically since then. Upon physical examination, body temperature of 38° C was observed, normal colored mucous membranes, heart and respiratory rates within normal limits with 2 seconds of capillary refill time (CRT), and no levels of dehydration, changes in lymph nodes or sensitivity to pain. abdominal. A complete blood count was performed, which indicated slight changes in the red series and thrombocytopenia, and material was sent for the diagnosis of hemoparasitosis using the Polymerase Chain Reaction (PCR) technique, which was found to be positive for Ehrlichia spp and Anaplasma spp. Specific treatment was applied with doxycycline and imidocarb dipropionate. During treatment, a new blood count was performed, which indicated improvement in the alterations and, two months after starting treatment, a new PCR was performed, in which results were negative for infectious agents.