Response Capacity Research Articles

Construction of a new emergency management system for potentially viral/viral municipal solid waste in China: Based on the "Beam-Column Structure" framework model

Emergency management of municipal solid waste during public health emergencies is crucial for curbing the spread of viruses and controlling environmental pollution. Most existing solid waste management systems, are designed with a top-down approach, covering multiple industries and sectors. However, these systems often fail to effectively manage potentially viral solid waste in household waste, which impedes effective solid waste management during public health emergencies and increases the risk of viral outbreaks. For example, during the COVID-19 outbreak, negligent management of potentially viral solid waste may result in the formation of a potential transmission chain of COVID-19 virus. Improper waste management may contaminate natural vectors such as water, soil and air, thereby increasing the pathways of human contact with infected vectors and promoting the risk of transmission of viral diseases such as dengue, Zika and hepatitis A. In this study, the applicability and limitations of the Chinese public emergency management system in the disposal of Potentially Viral/Viral Municipal Solid Waste (PVMSW) were analyzed in the breakout of public health crises. To address the need for precise risk control, efficient collaboration among key stakeholders, functional integration crossing multiple management organizations and flexible responses to complex situations, we proposed a PVMSW emergency management system based on a “Beam-Column Structure” model. This system consists of four modules: main traction, organizational leadership, functional articulation and demand matching. Each module is supported by the corresponding safeguards, including multi-level subject responsibilities, multi-scenario organization and implementation, multi-chain process connection and multi-demand control methods. The PVMSW emergency management system proposed in this study contributes to extending the framework of existing waste management protocols and enhancing the timely collection, transportation and disposal of PVMSW in public health crises. It offers valuable insights and guidance on the practices for improving risk awareness and management capacity in China's solid waste emergency response.

Impact of Combined Exercise and High-Intensity Interval Training on 6-Minute Walk Test Dynamics in Heart Failure Patients with Preserved Ejection Fraction: Results from the ExIC-FEp Trial

(1) Background: Heart failure with preserved ejection fraction (HFpEF) is a clinical challenge, with limited success from current therapeutic strategies. This study aimed to assess the impact of combined exercise (CE) and high-intensity interval training (HIIT) on key biomechanical parameters during the 6-minute walk test (6MWT) in HFpEF patients. (2) Methods: A randomized controlled trial was conducted in 76 sedentary HFpEF patients, divided into three groups as follows: the CE, HIIT, and control groups. CE involves aerobic and strength training, whereas HIIT includes high-intensity intervals. Cadence (steps per minute), distance, maximum heart rate (HRmax), and exercise intensity were measured pre- and postintervention. (3) Results: The results revealed significant improvements in cadence in the CE group compared with the control group (p = 0.009) for intention-to-treat analysis, and in the control and HIIT groups (p = 0.002) for per-protocol analysis. CE also significantly improved HRmax and exercise intensity, suggesting an enhanced cardiovascular response. In contrast, HIIT did not significantly affect these parameters. The correlation between cadence and distance covered during the 6MWT was strong in the overall group (r = 0.762, p < 0.001). (4) Conclusions: These findings suggest that CE is an effective intervention to improve gait efficiency, cardiovascular response, and functional capacity in HFpEF patients, whereas HIIT may require further investigation in this population.

Enhanced cell stress response and protein degradation capacity underlie artemisinin resistance in Plasmodium falciparum.

Malaria remains a global health burden, killing over half a million people each year. Decreased therapeutic efficacy to artemisinin, the most efficacious antimalarial, has been detected in sub-Saharan Africa, a worrying fact given that over 90% of deaths occur on this continent. Mutations in Kelch13 are the most well-established molecular marker for artemisinin resistance, but these do not explain all artemisinin-resistant isolates. Understanding the biological underpinnings of drug resistance is key to curbing the emergence and spread of artemisinin resistance. Artemisinin-mediated non-specific alkylation leads to the accumulation of misfolded and damaged proteins and activation of the parasite unfolded protein response (UPR). In addition, the parasite proteasome is vital to artemisinin resistance, as we have previously shown that chemical inhibition of the proteasome or mutations in the β2 proteasome subunit increase parasite susceptibility to dihydroartemisinin (DHA), the active metabolite of artemisinins. Here, we investigate parasites with mutations at the Kelch13 and/or 19S and 20S proteasome subunits with regard to UPR regulation and proteasome activity in the context of artemisinin resistance. Our data show that perturbing parasite proteostasis kills parasites, early parasite UPR signaling dictates DHA survival outcomes, and DHA susceptibility correlates with impairment of proteasome-mediated protein degradation. Importantly, we show that functional proteasomes are required for artemisinin resistance in a Kelch13-independent manner, and compound-selective proteasome inhibition demonstrates why artemisinin-resistant Kelch13 mutants remain susceptible to the related antimalarial peroxide OZ439. These data provide further evidence for targeting the parasite proteasome and UPR to overcome existing artemisinin resistance.IMPORTANCEDecreased therapeutic efficacy represents a major barrier to malaria treatment control strategies. The malaria proteasome and accompanying unfolded protein response are crucial to artemisinin resistance, revealing novel antimalarial therapeutic strategies.

Azobenzene-Functionalized Semicrystalline Liquid Crystal Elastomer Springs for Underwater Soft Robotic Actuators.

As actuated devices become smaller and more complex, there is a need for smart materials and structures that directly function as complete mechanical units without an external power supply. The strategy uses light-powered, twisted, and coiled azobenzene-functionalized semicrystalline liquid crystal elastomer (AC-LCE) springs. This twisting and coiling, which has previously been used for only thermally, electrochemically, or absorption-powered muscles, maximizes uniaxial and radial actuation. The specially designed photochemical muscles can undergo about 60% tensile stroke and provide 15kJ m-3 of work capacity in response to light, thus providing about three times and two times higher performance, respectively, than previous azobenzene actuators. Since this actuation is photochemical, driven by ultraviolet (UV) light and reversed by visible light, isothermal actuation can occur in a range of environmental conditions, including underwater. In addition, photoisomerization of the AC-LCEs enables unique latch-like actuation, eliminating the need for continuous energy application to maintain the stroke. Also, as the light-powered muscles processed to be either homochiral or heterochiral, the direction of actuation can be reversed. The presented approach highlights the novel capabilities of photochemical actuator materials that can be manipulated in untethered, isothermal, and wet environmental conditions, thus suggesting various potential applications, including underwater soft robotics.

Spatio-Temporal Analysis of Urban Emergency Response Resilience During Public Health Crises: A Case Study of Wuhan

In recent years, public health emergencies have severely disrupted city functions and endangered residents’ health and lives, enhancing the emergency response capacity, a crucial aspect of building resilient cities. Based on the Wuhan Statistical Yearbook and local economic and social big data, this study constructed a resilience assessment system that covers resistance, adaptability, and resilience. Evaluate the resilience level of each region and analyze its spatiotemporal characteristics using the entropy weight method, Theil index, and natural breakpoint method. The central area exhibited higher resilience levels, while peripheral areas showed lower resilience, owing to location advantage and developmental disparities. The obstacle degree model revealed that scientific and technological innovation, social rescue capabilities, and population size are the primary weak links in building urban emergency response capacity. Based on these findings, this study aims to provide policy recommendations and improvement measures for multiple stakeholders, guide the construction of resilient cities, enhance the ability to respond to public health crises and recovery speed, and ensure urban functions and residents’ well-being.

ADAM10 modulates the efficacy of T-cell-mediated therapy in solid tumors.

T-cell-mediated therapeutic strategies are the most potent effectors of cancer immunotherapy. However, an essential barrier to this therapy in solid tumors is disrupting the anti-cancer immune response, cancer-immunity cycle, T-cell priming, trafficking and T-cell cytotoxic capacity. Thus, reinforcingtheanti-cancer immune response is needed to improve the effectiveness of T-cell-mediated therapy. Tumor-associated protease ADAM10, endothelial cells (ECs) and cytotoxic CD8+ Tcells engage in complex communication via adhesion, transmigration and chemotactic mechanisms to facilitate an anti-cancer immune response. The precise impact of ADAM10 on the intricate mechanisms underlying these interactions remains unclear. This paper broadly explores how ADAM10, through different routes, influences the efficacy of T-cell-mediated therapy. ADAM10 cleaves CD8+ T-cell-targeting genes and impacts their expression and specificity. In addition, ADAM10 mediates the interactions of adhesion molecules with Tcells and influences CD8+ T-cell activity and trafficking. Thus, understanding the role of ADAM10 in these events may lead to innovative strategies for advancing T-cell-mediated therapies.

Big cities, big bodies: urbanisation correlates with large body sizes and enhanced body condition in African dwarf chameleons (Genus: Bradypodion)

Urbanisation is a major driver of habitat transformation that alters the environmental conditions and selective regimes of the habitats where it occurs. For species inhabiting urban habitats, such alterations can facilitate adaptive responses in their phenotypes, including their morphology. Quantifying potential responses could provide important information for assessing adaptation to urbanisation and may also be relevant to their conservation. Previous studies on African dwarf chameleons (Bradypodion) have shown these lizards to have remarkable adaptive capacity in response to different habitats (e.g. closed canopy habitats vs. open canopy habitats). Several of these species exploit urban habitats, but the extent to which populations are adapting to urban environments has only recently started to receive attention. In this study, we quantify differences in body size and body condition between urban and natural populations of five species of dwarf chameleons. For most comparisons, either females, males or both sexes from urban populations were longer, heavier and/or had better body condition than those from natural populations. In the remaining cases, there were no differences in these traits between populations. Our findings conform with the emerging paradigm that urbanisation positively correlates with enhanced lizard body sizes and condition, although the reasons for this may be complex. Nevertheless, our data provide an avenue for future research into investigating the potential factors (e.g. food supply, predation risk, etc.) that facilitate the trends we observed.

Community Participation in Flood Management Systems in Aceh Utara Regency

Flooding remains a persistent challenge in Aceh Utara Regency, representing a complex, cross-sectoral issue. If not addressed through collaboration among various stakeholders, including community groups, it has the potential to lead to new societal problems. This research seeks to assess the effectiveness and impact of using a participatory model in flood management efforts in the region. A qualitative approach was employed, gathering data through in-depth interviews with stakeholders such as local communities, government authorities, and non-governmental organizations involved in flood mitigation programs. The findings emphasize the urgency of adopting a participatory model to enhance the effectiveness of flood management systems. Actively engaging the community in planning, execution, and evaluation processes leads to a deeper understanding of local conditions, needs, and suitable solutions. Key aspects of the participatory model include raising public awareness of flood risks and mitigation measures, improving flood management infrastructure based on community input, creating more accessible and effective evacuation plans, and strengthening the community’s capacity for emergency response and simple mitigation efforts. In conclusion, this study aims to further explore the relevance and effectiveness of the participatory model in flood management in Aceh Utara Regency, ensuring the long-term sustainability of the region's disaster management system.

Operational reliability and non-deterministic resilience estimation of active distribution network incorporating effect of real-time dynamic hosting capacity

Active distribution networks are increasingly recognized essential for achieving sustainable development goals. Traditionally, hosting capacity was considered as a static measure for planning distributed energy resources integration. This work introduces the concept of dynamic hosting capacity, which recurrently re-evaluates hosting capacity in response to erratic modern grid conditions. The introduction of dynamic hosting capacity facilitated testing variations of power injection from minimum to 100 %, sustaining power system governing parameter limits. This embarked the need of operational reliability assessment and enhancing situational awareness for optimum power injection and balance. To achieve operational reliability analysis based on dynamic hosting capacity, hybrid probability distribution function-based Monte Carlo simulation is proposed. This resulted in 85–90 %. improvisation of solar photovoltaic generation and load alignment, as this methodology provides comprehensive and accurate assessment of system performance under diverse uncertainties. The framework's validation includes projection of time-varying operational reliability indices, over time independent reliability indices i.e., dynamic loss of load probability, dynamic loss of load expectation, dynamic loss of load duration, dynamic loss of load frequency, dynamic grid margin, and dynamic grid dependency. This resulted in 30 % improvement in assessment of grid margin, facilitating reliable uncertainty handling competence. Additionally, expectation maximization algorithm is proposed to evaluate non-deterministic resilience due to ambiguities associated with solar photovoltaic distributed energy resources. The non-deterministic resilience assessment testified 80 % bounce-back rate, demonstrating better adaptability and robustness. The entire analysis is conducted in MATLAB, validated using Typhoon Hardware-in-Loop real-time platform, and compared with existing literatures to demonstrate its effectiveness.

Propionate Decreases Microglial Activation but Impairs Phagocytic Capacity in Response to Aggregated Fibrillar Amyloid Beta Protein.

Microglia, the innate immune cell of the brain, are a principal player in Alzheimer's disease (AD) pathogenesis. Their surveillance of the brain leads to interaction with the protein aggregates that drive AD pathogenesis, most notably Amyloid Beta (Aβ). Microglia attempt to clear and degrade Aβ using phagocytic machinery, spurring damaging neuroinflammation in the process. Thus, modulation of the microglial response to Aβ is crucial in mitigating AD pathophysiology. SCFAs, microbial byproducts of dietary fiber fermentation, are blood-brain barrier permeable molecules that have recently been shown to modulate microglial function. It is unclear whether propionate, one representative SCFA, has beneficial or detrimental effects on microglia in AD. Thus, we investigated its impact on microglial Aβ response in vitro. Using a multiomics approach, we characterized the transcriptomic, metabolomic, and lipidomic responses of immortalized murine microglia following 1 h of Aβ stimulation, as well as characterizing Aβ phagocytosis and secretion of reactive nitrogen species. Propionate blunted the early inflammatory response driven by Aβ, downregulating the expression of many Aβ-stimulated immune genes, including those regulating inflammation, the immune complement system, and chemotaxis. Further, it reduced the expression of Apoe and inflammation-promoting Aβ-binding scavenger receptors such as Cd36 and Msr1 in favor of inflammation-dampening Lpl, although this led to impaired phagocytosis. Finally, propionate shifted microglial metabolism, altering phospholipid composition and diverting arginine metabolism, resulting in decreased nitric oxide production. Altogether, our data demonstrate a modulatory role of propionate on microglia that may dampen immune activation in response to Aβ, although at the expense of phagocytic capacity.

Resource Adequacy and Integration of Renewables in Light of US, EU, and Pakistan’s Evolving Power Sector

This study investigates resource adequacy and renewable energy integration in the United States, European Union, and Pakistan amid global energy market liberalization and greenhouse gas reduction efforts. It explores how these regions are adapting to the surge in renewable sources like wind and solar, which, despite their financial and environmental benefits, challenge resource adequacy and the economic viability of traditional energy sources. In the US and EU, significant improvements have been introduced in wholesale electricity markets and capacity accreditation mechanisms, which enhanced the large-scale deployment of renewables. This shift has prompted a reevaluation of resource adequacy, leading to the increased deployment of battery storage and demand response. Presently, gas-based generation is largely upholding resource adequacy; however, future trends indicate a move towards greater consumer participation, energy efficiency, and utility-scale storage, with a decline in fossil fuel use. Pakistan aims to adopt a liberalized market structure by balancing competitive markets with legacy contracts. Public pressure is driving a shift from costly fossil-based generation to renewables. Similarly, a trend in the rise of behind-the-meter solar generation can be witnessed. In the future, Pakistan may also experience resource adequacy challenges. It will likely need to implement battery storage, demand response, and modern capacity accreditation tools, by drawing lessons from developed markets.

Weather shocks affect trade policy: Evidence from preferential trade agreements

AbstractWe show that weather shocks affect government trade policy decisions. We exploit variation in rainfall during the growing season within and across countries to show that weather shocks impact agricultural output and trade. Using information on tariff cuts by commodity from preferential trade agreements signed between 1995 and 2014, we then show that weather shocks that happen during the negotiation period correlate strongly with the size of tariff cuts. When weather shocks increase (decrease) a country's capacity to produce a given crop, its government negotiates a smaller (larger) tariff cut. These results are consistent with a political economy trade model with sector‐specific inputs in which the government places more weight on producers relative to consumers in its objective function. They also reveal that governments update their beliefs about domestic agricultural production capacity in response to weather shocks.

Detection and Localization of IL-8 and CXCR1 in Rainbow Trout Larvae in Response to Pseudomonas aeruginosa Lipopolysaccharide

The salmonid industry faces challenges due to the susceptibility of fish to opportunistic pathogens, particularly in early developmental stages. Understanding the immunological capacity during these stages is crucial for developing effective disease control strategies. IL-8R, a member of the G-protein-coupled receptor family, acts as a receptor for Interleukin 8 (IL-8). The binding of IL-8 to IL-8R plays a major role in the pathophysiology of a wide spectrum of inflammatory conditions. This study focused on the immune response capacity of rainbow trout (Oncorhynchus mykiss) larvae by analyzing IL-8/CXCR1 response to lipopolysaccharide (LPS) from Pseudomonas aeruginosa. Previous research demonstrated that LPS from P. aeruginosa acts as a potent immunostimulant in teleost, enhancing pro-inflammatory cytokines. The methodology included in silico analysis and the synthesis and characterization of an omCXCR1-derived epitope peptide, which was used to produce omCXCR1-specific anti98 serum in mice. The research revealed that rainbow trout larvae 19 days post-hatching (dph) exhibited pronounced immune responses post-stimulation with 1 µg/mL of LPS. This was evidenced by the upregulated protein expression of IL-8 and omCXCR1 in trout larvae 2 and 8 h after LPS challenge, as analyzed by ELISA and immunohistochemistry. Furthermore, fluorescence microscopy successfully revealed the colocalization of IL-8 and its receptor in cells from mucosal tissues after LPS challenge in larvae 19 dph. These findings underscore the efficacy of LPS immersion as a method to activate the innate immune system in trout larvae. Furthermore, we propose IL-8 and its receptor as molecular markers for evaluating immunostimulation in the early developmental stages of salmonids.

Integrating Nature-Based Solutions for urban stormwater management into existing urban fabrics

Abstract Nature-Based Solutions (NBSs) have been proven to address urban stormwater challenges effectively while providing multiple benefits to the economy, society, and environment. Urban fabrics profoundly influence flood risk as the changes in urban form, land use, and building layout will affect the urban hydrological cycle, thereby altering precipitation and runoff. However, few studies look at the connection between NBSs and urban fabrics, which can contribute to urban stormwater management. This paper aims to categorize the general types of urban fabrics and consider NBSs as strategic interventions that can improve water-related issues and help to create a resilient neighborhood. This paper elaborates on some strategic approaches to integrate NBSs in existing urban fabrics at the neighborhood scale to optimize their response capacity according to the local conditions. The novelty lies in adopting a systemic vision to deliver a design toolkit that can be adapted to different conditions and with different typologies across the places and countries facing similar water-related challenges. In conclusion, this paper emphasizes the importance of understanding how different NBSs fit various urban fabric types, and future work is needed to provide assessment tools to evaluate the efficiency of different design scenarios.

Two-stage low-carbon economic dispatch of an integrated energy system considering flexible decoupling of electricity and heat on sides of source and load

Under the goal of "double carbon", in order to further enhance the level of new energy consumption and solve the problem of restricting the flexibility of the system by "ordering power by heat" of combined heat and power (CHP) units, a low-carbon economic planning strategy with flexible decoupling of electricity and heat is proposed, by introducing a new type of electric-thermal coupling equipment on both sides of the source and load. Firstly, Consideration of the low-carbon and environmentally friendly characteristics of green ammonia production and ammonia-doped combustion technologies, a wind power(WT) – power to ammonia(P2A) - CHP units - thermal power (TH) units joint operation strategy is proposed on the source side. This strategy realizes the conversion of abandoned wind to green ammonia to ammonia coal hybrid generation, the decoupled operation of CHP units and promotes the consumption of wind power and the low-carbon operation of the system. Secondly, A dynamic incentive demand response model is developed to meet the demand of high proportion distributed PV in situ consumption on the load side. The dynamic incentive price guides the distributed power-to-heat load to change the response capacity, tracks the abandonment of wind and light, realizes the flexible conversion of power and heat load, and cooperates with the source side to promote the coupling operation of electric pyrolysis. On this basis, consider the flexible decoupling capability of electric-heat coupling equipment on both sides of the source and load to establish a two-phase low-carbon scheduling model for the day-before and day-after phases. In the day-ahead phase, the source-side electric-thermal-ammonia joint operation strategy is considered, and the electric and thermal energy supply plans are adjusted centrally; In the intra-day phase, the flexible adjustment range of power-to-heat devices and the heat load inertia on the load side are taken into account, and the electricity and heat planning strategies are adjusted in a distributed-centralised manner in conjunction with the source side. Finally, through the simulation of different cases, the results show that compared with the traditional electric heating system, the total cost of the system considering the scheduling strategy proposed in this paper decreases by ￥826,900, the carbon emission decreases by 1.2t, and basically realises the consumption of wind power and distributed photovoltaic power output. The proposed scheme reduces carbon emissions, promotes the consumption of wind power and distributed photovoltaic output, and is able to reach the goal of low-carbon economic dispatch.

Disaster Preparedness among Households in the Municipality of Pura, Tarlac: An Analysis

This research endeavors to evaluate the level of disaster preparedness among households in the Municipality of Pura, Tarlac, employing a quantitative descriptive research approach. A survey questionnaire was utilized to gather data from 112 household respondents, selected through random sampling across barangays most vulnerable to disasters. Data analysis, facilitated by SPSS analytical statistics software, focused on identifying trends and correlations between disaster preparedness levels and encountered challenges. The findings reveal both strengths and challenges in disaster preparedness. While emergency plans, risk awareness, and response capacity demonstrate commendable efficacy, deficiencies in emergency supplies, community engagement, and infrastructure persist. Correlation analysis underscores the positive relationship between proactive measures and mitigation of challenges, emphasizing the importance of investing in comprehensive disaster management strategies. Recommendations are proposed based on the research findings. Strengthening emergency supplies and resources through equitable distribution mechanisms and community collaboration is advised. Fostering greater community engagement and collaboration, enhancing emergency plans, and improving response and recovery capacity are also recommended. These measures, encompassing workshops, collaboration initiatives, and resource provisions, aim to empower households with the necessary knowledge and resources for effective disaster preparedness, response, and recovery. Implementation of these recommendations is crucial for enhancing disaster resilience and readiness in the Municipality of Pura, Tarlac. By addressing identified challenges and bolstering proactive measures, the community can better safeguard its residents and infrastructure against future disasters, ensuring their safety and well-being.

경찰공무원의 직무몰입이 재난대응역량에 미치는 영향에서 회복탄력성의 매개효과

Modern society faces many forms of disasters, not just emerging viral infectious diseases like COVID-19. These disasters not only cause huge losses in human and material resources, but also cause social disruption. Effective disaster response is an essential part of protecting society and its people, and the role of police officers is pivotal in this process. Police officers are directly involved in maintaining order, saving lives, and recovering from disasters during the initial response phase, so their response capabilities have a direct impact on the effectiveness of disaster management. In this context, it can be argued that police officer engagement plays a key role in enhancing disaster response capacity. To further understand the relationship between job commitment and disaster response capacity, it is also necessary to consider the internal factors of individuals. In this study, we propose resilience as such an internal factor and explore its mediating effect to enhance the effectiveness of the police role in disaster situations. The results of the analysis showed that job commitment (=.66, p<.001) was positively significant as a result of the significance test of the effect of the independent variable on the mediating variable. On the other hand, the results of the significance test of the effects of independent variables and parameters on the dependent variable showed that job commitment (=.29, p<.001) had a significant effect on disaster response capability, and the parameter resilience also had a significant effect on disaster response capability (=.33, p<.001). The results suggest that police officers' job engagement directly enhances their disaster response capabilities and that resilience partially influences this relationship. This suggests that police officers with higher job commitment have the ability to respond more effectively in disaster situations. These findings suggest that programs that promote resilience in police organizations can enhance job commitment, which in turn can improve the disaster response capabilities of police officers. Therefore, police organizations should consider implementing a variety of training and support programs to enhance resilience in addition to strategies to increase job engagement.

NQO1 from Litopenaeus vannamei involved in the regulation of antioxidant capacity, inflammation, and apoptosis

NAD(P)H:quinone oxidoreductase 1 (NQO1) is a versatile FAD-dependent flavoprotein and a key regulator in the Nrf2/ARE signaling pathway. In this study, we cloned the NQO1 gene of Litopenaeus vannamei (LvNQO1) and investigated its role in immune regulation. The results showed that the LvNQO1 gene has an ORF of 987 bp, encoding 328 amino acids. The protein contains an NQO family domain. Homology and phylogenetic analysis revealed that LvNQO1 shares similarities with the NQO1 of Marsupenaeus japonicus, clustering into the same clade. LvNQO1 was expressed in 10 tissues of L. vannamei, with the highest expression in the hepatopancreas and the lowest in the intestine. After RNA interference (RNAi) of LvNQO1, the expression of antioxidant genes (SOD and GST) initially increased but then decreased after 24 h. The expression levels of Gpx and CAT decreased early on but gradually recovered. Immune-related factors (Toll4, JNK, and Hsp90) and apoptosis-related factors (Caspase3, P53, and bcl2) were upregulated. Following RNAi of LvNQO1, Vibrio harveyi challenge significantly increased the expression of immune factors (JNK and HIF1α) and apoptosis factors (Caspase3, P53, and bcl2) in the hepatopancreas. Toll 4 receptors recognized the infection, upregulated their expression, and activated an immune response. RNAi of LvNQO1 led to noticeable histological lesions and apoptosis. These findings suggest that LvNQO1 may play a role in regulating antioxidant capacity, inflammation, and apoptosis in response to Vibrio infection.

Assessment of the effectiveness of hospital external disaster functional drills on health care receivers’ performance, using standardized patients and mass cards simulation: a pilot study from Saudi Arabia

BackgroundGiven the increasing frequency of disasters globally, it is critical that healthcare systems are prepared for these mass casualty events. The Saudi health system’s preparedness for mass casualty incidents needs to be more robust, potentially due to limited disaster drills and inadequate standardized patient (SP) simulation training. This study aims to (i) assess the performance of front-line hospital staff in Saudi Arabia through a functional drill and (ii) evaluate the drill’s effectiveness using SP and MAC-SIM cards, providing detailed insights into its design and execution.MethodsA functional drill was conducted at a government hospital in Riyadh, Saudi Arabia, on December 19, 2022, using a cross-sectional approach with two phases. 141 healthcare receivers served as subjects, while 23 volunteers acted as SPs. The scenario simulated a building collapse to assess the emergency department (ED) response, interdepartmental communication, and surge capacity. Data were collected through direct observation of healthcare practitioners’ interactions with the SPs, analysis of SP data, and participant feedback. Quantitative data were analyzed descriptively, while qualitative data were examined for patterns and themes related to simulation performance and effectiveness.ResultsThe hospital receivers’ performances demonstrated accurate triage categories. The ED team assessed most patients (67%) in less than 5 min. For patients requiring definitive care, such as intensive care unit, 95% spent less than 2.5 h in the ED. Most patients (65%) required ‘other treatments’. Communication was efficient in the triage zone and the yellow treatment area. Participants’ feedback on using MAC-SIM cards during the simulation was overwhelmingly positive with 82.61% reporting that MAC-SIM use helped them respond better. Experienced SPs (paramedics) with prior disaster knowledge and experience outperformed inexperienced SPs (nurses) in the functional exercise.ConclusionThis groundbreaking study is the first in the Arabic Gulf region to use SPs with MAC-SIM cards in functional drills. The findings highlight the potential of simulation exercises to improve hospital team knowledge and performance when responding to disasters. Multiple evaluation techniques can effectively identify participant strengths and weaknesses, informing future disaster improvement plans. This information is a valuable resource for Arabic and middle-income countries where disaster medicine is still developing.

Assessing the Implications of Deforestation and Climate Change on Rural Livelihood in Ghana: a Multidimensional Analysis and Solution-Based Approach.

The Ashanti region in Ghana, abundant in natural resources such as forests and vegetation biomes, significantly supports the livelihoods of a significant portion of the population. The sustainable management of forest resources remains a significant challenge to achieving environmental and economic growth and poverty alleviation. The study aims to identify the drivers of deforestation and assess its impact on the livelihoods of the poor and vulnerable communities in the Ashanti region. The study utilized qualitative and space-based data to examine the patterns of vegetation cover and deforestation from 2000 to 2020. The results revealed moderate to sparse vegetation in Ashanti from 2002, 2005, 2011, 2015, 2017, and 2018, with no vegetation in the northcentral part, attributed to climate change, agricultural practices, government policies, and deforestation-related disasters. The study found a significant correlation (R² = 0.8197) between years and deforestation areas, especially in 2018 at around 16,000 Sqkm, indicating an exponential increase with severe implications for sustainable livelihoods. Much of these changes were reflected in 2020 with a high peak of deforestation towards the southeastern parts of the region. Additionally, the results show that the poor groups are not passive actors but are actively involved in identifying systems and processes through which to build their adaptive capacity and resilience to environmental and climate change-induced changes. The findings provide evidence-based and all-inclusive approaches that would encourage vulnerable and marginalized groups to participate in the co-production and co-creation of policies and strategies. This outcome is geared towards transformative and sustainable communities while ensuring efficient and effective response and recovery capacities of deforested lands.