Self-organizing Capacity Research Articles

Stable and Thermo-Responsive Dextran Sulfate-Graft-PNIPAm Amphiphilic Nanoparticles for Potential Target Methotrexate Delivery

Thermo-responsive copolymers grafted with N-isopropylacrylamide (NIPAm) are excellent candidates for drug release. Dextran sulfate (DS) acts as a specific ligand in inflamed regions, turning it highly useful as a target for drug delivery. DS was associated with NIPAm to produce amphiphilic graft copolymers prepared via free radicals. The molar ratio of feed reagents NIPAm/DS varied from 1 (DS-g-PNIPAm) to 4 (DS-g-4PNIPAm). The synthesis was confirmed by spectroscopic techniques (Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR)). All copolymers showed self-organization capacity in an aqueous medium in temperatures higher than 34 °C, and sizes less than 300 nm. DS-g-3PNIPAm exhibited stability in water and in phosphate buffer at pH 7.4. Scanning electron microscopy confirmed their spherical shape. This copolymer showed specificity to leukemic cells, and normal cells’ proliferation. Methotrexate (MTX) is a very low water-soluble drug used for rheumatoid arthritis and cancer. Unfortunately, MTX have severe collateral effects. MTX-loaded nanoparticles can overcome such issues as well as enhance bioactivity and stability. The MTX was encapsulated and delivered from the DS-g-3PNIPAm with potential target delivery due to the presence of DS. Comparison with MTX encapsulated in other nanoparticles reveals that the DS-g-PNIPam presents the best performance among the thermoresponsive and the second among the target MTX nanocarriers.

Population resilience to flooding in the urbanised mangrove of Douala, Cameroon

PurposeThis study aims to understand the mechanisms that contribute to the construction of the resilience of populations to urban flooding. It therefore proposes to analyse the strategies and attitudes developed by households subjected to floods to maintain and rebuild themselves despite the damage in the urbanised mangrove swamp of the south of the city.Design/methodology/approachThe fieldwork consisted of observing the behaviours, practices and attitudes of households to flooding in the study area. In addition to observations, the fieldwork included interviews (60), focus groups (10), completion of 260 questionnaires by heads of households and field mapping. The sample was determined by the non-probability quota method. The data processing involved the extraction of descriptive and multivariate statistics as well as content analysis.FindingsThe rapid growth of Douala leads to vulnerabilities through the spontaneous occupation of mangrove areas, thus aggravating the impacts of the floods that occur. Afterwards, the analysis of the individual and collective responses of the inhabitants of the study area highlighted resilience in action. This resilience is expressed in two ways: the individual ability of the inhabitants to exploit internal/psychological and external resources/personal social capital to absorb the shock; and the capacity for self-organisation and learning which is manifested by the use of feedback in the reconstruction phase to transform the disaster into an opportunity for territorial development.Research limitations/implicationsTwo main limitations of this work should be noted and are as follows. The study is only centred on an urbanised mangrove area, although there are others: Does the research hypothesis also apply to other urbanised mangrove areas? Flooding is not specific to mangrove areas. Other spontaneous non-mangrove neighbourhoods are subject to flooding. The results of this study give the impression that resilience capacities are paradoxically manifested in the most vulnerable neighbourhoods. This impression needs to be confirmed by further studies for a comparative synthesis of the experience of shocks between spontaneous neighbourhoods and planned neighbourhoods.Practical implicationsUrban flooding is one of many physical phenomena that scientists and technologists are learning about and trying to anticipate, avoid, and prepare for. Governments are simultaneously implementing policies for prevention, monitoring, warning, and reaction. If this analysis does not take into account the intrinsic capacities of the populations to cope with dangers, accept responsibility for their actions, and take significant local action when necessary, this framework will still be inadequate. Spontaneous activities are gradually developing around these efforts into coordinated, structured actions that, if taken into consideration and effectively managed, can prove to be successful and satisfying on a town-wide scale.Social implicationsScientists and technicians are learning about physical phenomena such as urban flooding and are seeking ways to predict, prevent and protect against them. In parallel, governments are putting in place prevention, monitoring, warning and response policies. This framework is still lacking if this study does not consider the populations’ inherent abilities to deal with threats, take responsibility for their actions and take meaningful local action when necessary. Around these efforts, spontaneous initiatives are progressively emerging into concerted structured actions that can prove to be effective and satisfactory on a town-wide scale if they are taken into account and well managed.Originality/valueVery few studies have considered the forms of expression of resilience in marginal urban environments such as spontaneous and anarchic neighbourhoods, non-aedificandi zones and sensitive environments such as the mangroves areas.

Impact of Natural Disaster Shocks on Farm Household Poverty Vulnerability—A Threshold Effect Based on Livelihood Resilience

Poverty caused by disasters poses a great challenge to consolidate the achievements of poverty alleviation. Livelihood resilience is the key factor for farmers to resist risks and get rid of poverty. Therefore, this study used the China Family Panel Studies (CFPS) database. Firstly, we examined the impact of natural disasters on the poverty vulnerability of farmers. Secondly, taking livelihood resilience and its decomposition dimensions as threshold variables, we examined the mechanism of livelihood resilience between natural disasters and poverty. The results show that natural disaster shocks, natural disaster intensity, and natural disaster frequency all had a significant positive effect on farm households’ vulnerability to poverty. The threshold test shows that natural disasters had larger effects on the poverty vulnerability of the farmers with lower buffer capacity, self-organizing capacity, and learning capacity. When the livelihood resilience value exceeded the third threshold, the impact of natural disasters on the poverty vulnerability of farmers turned from positive to negative. When the buffer capacity exceeded the third threshold, the impact of natural disasters on poverty vulnerability turned from positive to negative; when the self-organizing capacity exceeded the first threshold, the impact of natural disasters on poverty vulnerability turned from positive to negative; when the learning capacity exceeded the third threshold, the impact of natural disasters on poverty vulnerability turned from positive to negative. Therefore, it is suggested that appropriate policies should be needed to support farmers’ livelihood resilience and address disaster-induced poverty by improving farmers’ buffer capacity, self-organizing capacity, and learning capacity. Focusing on farmers’ livelihood resilience, government should establish a policy support system aimed at improving farmers’ buffer capacity, self-organizing capacity, and learning capacity, that will help farmers to escape from disaster-induced poverty.

Bioengineering the human bone marrow microenvironment in liquefied compartments: A promising approach for the recapitulation of osteovascular niches

Recreating the biological complexity of living bone marrow (BM) in a single in vitro strategy has faced many challenges. Most bioengineered strategies propose the co-culture of BM cellular components entrapped in different matrices limiting their migration and self-organization capacity or in open scaffolds enabling their escaping. We propose a methodology for fabricating a “human bone marrow–in–a–liquefied-capsule” to overcome these challenges, embracing the most important BM components in a single platform. Since free dispersion of the cells within the BM is an essential feature to maintain their in vivo properties, this platform provides a liquefied environment for the encapsulated cells to move freely and self-organize. Inside liquefied capsules, an engineered endosteal niche (eEN) is co-cultured with human umbilical cord cells, including endothelial cells and hematopoietic stem and progenitor cells (HSPCs). Two different human-like BM niches were recreated under static and dynamic systems. Although the culture of the engineered BM capsules (eBMC) in these different environments did not change the structural and compositional features of the BM niches, the biophysical stimulation potentiated the cellular intercommunication and the biomolecules secretion, demonstrating an enhanced in vitro bio performance. Moreover, while the eBMC without HSPCs provided the secretion of hematopoietic supportive factors, the presence of these cells recapitulated more closely the biological complexity of the native BM niches. This functional eBMC approach is an innovative platform capable of investigating several components and interactions of BM niches and how they regulate BM homeostasis and hematopoiesis. Statement of significanceThe recapitulation of the multifaceted bone marrow (BM) microenvironment under in vitro conditions has gained intensive recognition to understand the intrinsic complexity of the native BM. While conventional strategies do not recapitulate the BM osteovascular niches nor give the cellular components a free movement, we report for the first time the development of human bone marrow–in–a–liquefied-capsule to overcome such limitations. Our engineered BM capsules (eBMC) partially mimic the complex structure, composition, and spatial organization of the native osteovascular niches present in the BM. This strategy offers a platform with physiological relevance to exploit the niches’ components/networks and how they regulate the hematopoiesis and the initiation/progression of various BM-related pathologies.

Livelihood Resilience or Policy Attraction? Factors Determining Households' Willingness to Participate in Rural Tourism in Western China.

Rural tourism in developing countries has been regarded as a means for rural development, environment conservation and poverty alleviation. This study aims to examine the determining factors for rural households’ willingness to participate in rural tourism in western areas under the context of China’s rural revitalization strategy. Based on survey data from 22 tourism poverty alleviation villages located in the poor areas of western China, we characterize our results by stating that rural households’ livelihood resilience “push” on their willingness to participate, and that poverty alleviation policy perception and involvement “pull” on it. Among distinct livelihood adaptive strategy groups, i.e., farming-oriented households, migratory-oriented households and tourism-participating households, this study also revealed that buffer capacity was a significant driving force for the three types of household’s willingness. Positive poverty alleviation policy perception could attract migratory households to return to their hometowns to start tourism businesses; however, better self-organizing capacity decreased their willingness. In addition, both self-organization and learning capacity and positive policy perception and involvement encouraged tourism-participating households to engage in tourism activities continually. Finally, some practical implications and recommendations for further research are also discussed.

Home Away From Home: Bioengineering Advancements to Mimic the Developmental and Adult Stem Cell Niche

The inherent self-organizing capacity of pluripotent and adult stem cell populations has advanced our fundamental understanding of processes that drive human development, homeostasis, regeneration, and disease progression. Translating these principles into in vitro model systems has been achieved with the advent of organoid technology, driving innovation to harness patient-specific, cell-laden regenerative constructs that can be engineered to augment or replace diseased tissue. While developmental organization and regenerative adult stem cell niches are tightly regulated in vivo, in vitro analogs lack defined architecture and presentation of physicochemical cues, leading to the unhindered arrangement of mini-tissues that lack complete physiological mimicry. This review aims to highlight the recent integrative engineering approaches that elicit spatio-temporal control of the extracellular niche to direct the structural and functional maturation of pluripotent and adult stem cell derivatives. While the advances presented here leverage multi-pronged strategies ranging from synthetic biology to microfabrication technologies, the methods converge on recreating the biochemical and biophysical milieu of the native tissue to be modeled or regenerated.

Hydrogel Mechanics Influence the Growth and Development of Embedded Brain Organoids.

Brain organoids are three-dimensional, tissue-engineered neural models derived from induced pluripotent stem cells that enable studies of neurodevelopmental and disease processes. Mechanical properties of the microenvironment are known to be critical parameters in tissue engineering, but the mechanical consequences of the encapsulating matrix on brain organoid growth and development remain undefined. Here, Matrigel was modified with an interpenetrating network (IPN) of alginate, to tune the mechanical properties of the encapsulating matrix. Brain organoids grown in IPNs were viable, with the characteristic formation of neuroepithelial buds. However, organoid growth was significantly restricted in the stiffest matrix tested. Moreover, stiffer matrixes skewed cell populations toward mature neuronal phenotypes, with fewer and smaller neural rosettes. These findings demonstrate that the mechanics of the culture environment are important parameters in brain organoid development and show that the self-organizing capacity and subsequent architecture of brain organoids can be modulated by forces arising from growth-induced compression of the surrounding matrix. This study therefore suggests that carefully designing the mechanical properties of organoid encapsulation materials is a potential strategy to direct organoid growth and maturation toward desired structures.

Novel Drug Screening Platform: Tumor Organoid

Pancreatic ductal adenocarcinoma (PDAC) is known to be one of the most lethal cancers among all cancer types, with a relative 5-year survival rate of less than 8%. Currently, surgery is the only probable curative treatment for PDAC which is available for only 10-15% of the patients diagnosed with the cancer. Organoids resemble the original tissue in morphology and function with self-organizing capacity. Organoids can be cultured with high effectiveness from individual patient derived tumor tissue which makes them an extremely fitting model for translational uses and the improvement of personalized cancer medicine. Before personalized medicine based on organoids can be applied in the clinic, the improvement of drug screening platforms in terms of sensitivity and robustness is necessary.

A study on livelihood resilience in the small-scale fisheries of Ghana using a structural equation modelling approach

Livelihood resilience is one of the social-ecological concepts with importance for development, policy and research as crises from human-environment interactions are becoming intense and widespread. Nevertheless, measuring livelihood resilience is a difficult task requiring handy and practical methods, which are missing in the literature. This study proposed and validated a measure to the three-dimensional livelihood resilience framework of buffer capacity, capacity for self-organization and learning in the context of Ghana's declining small-scale fisheries. It proposed measurement indicators to the three dimensions of the framework and tested its structural and predictive validity using simultaneous equations. The data used for the analysis were collected from a random sample of 1180 artisanal fisherfolk in three fishing communities using a semi-structured questionnaire. The framework is useful for livelihoods and resilience thinking and its three dimensions are uniquely interrelated and significantly explain the livelihood resilience of fisherfolks. Aside from the slight differences in the paths to livelihood resilience-building across communities observed, there were significant gender and community differences in the application of the framework. These findings suggest the usefulness of the framework for monitoring and evaluation of interventions aimed at building livelihood resilience of fisherfolks. However, the highlighted differences in its application across gender and community contexts need to be considered. The study contributes to sustainable fisheries and coastal management by providing insights on the pathways for enhancing livelihood resilience—reducing social vulnerability and promoting the adaptive capacities of fisherfolk to shocks. For instance, interventions towards building livelihood resilience could increase fisherfolk's access and ownership to capitals through trusted savings and lending schemes/associations and impact capitals (buffer capacity); institutionalization and strengthening traditional authority (self-organization); and framing advocacy messages against illegal fishing practices highlighting previous experiences of good fish catch under less pervasiveness of such illicit practices (learning).

LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells

The generation of mature ventricular cardiomyocytes (CMs) resembling adult CMs from human pluripotent stem cells (hPSCs) is necessary for disease modeling and drug discovery. To investigate the effect of self-organizing capacity on the generation of mature cardiac organoids (COs), we generated cardiac mesoderm cell-derived COs (CMC-COs) and CM-derived COs (CM-COs) and evaluated COs. CMC-COs exhibited more organized sarcomere structures and mitochondria, well-arranged t-tubule structures, and evenly distributed intercalated discs. Increased expressions of ventricular CM, cardiac metabolic, t-tubule formation, K+ ion channel, and junctional markers were confirmed in CMC-COs. Mature ventricular-like function such as faster motion vector speed, decreased beats per min, increased peak-to-peak duration, and prolonged APD50 and APD90 were observed in CMC-COs. Transcriptional profiling revealed that extracellular matrix-integrin, focal adhesion, and LEFTY-PITX2 signaling pathways are upregulated in CMC-COs. LEFTY knockdown affected ECM-integrin-FA signaling pathways in CMC-COs. Here, we found that high self-organizing capacity of CMCs is critical for the generation of mature and ventricular COs. We also demonstrated that LEFTY-PITX2 signaling plays key roles for CM maturation and specification into ventricular-like CM subtype in CMC-COs. CMC-COs are an attractive resource for disease modeling and drug discovery.

Gastric Organoids: Progress and Remaining Challenges.

The stomach is a complex and physiologically necessary organ, yet large differences in physiology between mouse and human stomachs have impeded translation of physiological discoveries and drug screens performed using murine gastric tissues. Gastric cancer (GC) is a global health threat, with a high mortality rate and limited treatment options. The heterogeneous nature of GC makes it poorly suited for current “one size fits all” standard treatments. In this review, we discuss the rapidly evolving field of gastric organoids, with a focus on studies expanding cultures from primary human tissues and describing the benefits of mouse organoid models. We introduce the differing methods for culturing healthy gastric tissue from adult tissues or pluripotent stem cells, discuss the promise these systems have for preclinical drug screens, and highlight applications of organoids for precision medicine. Finally, we discuss the limitations of these models and look to the future to present potential ways gastric organoids will advance treatment options for patients with GC.

Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells.

Multicellular organisms maintain structure and function of tissues/organs through emergent, self-organizing behavior. In this report, we demonstrate a critical role for lung mesenchymal stromal cell (L-MSC) aging in determining the capacity to form three-dimensional organoids or 'alveolospheres' with type 2 alveolar epithelial cells (AEC2s). In contrast to L-MSCs from aged mice, young L-MSCs support the efficient formation of alveolospheres when co-cultured with young or aged AEC2s. Aged L-MSCs demonstrated features of cellular senescence, altered bioenergetics, and a senescence-associated secretory profile (SASP). The reactive oxygen species generating enzyme, NADPH oxidase 4 (Nox4), was highly activated in aged L-MSCs and Nox4 downregulation was sufficient to, at least partially, reverse this age-related energy deficit, while restoring the self-organizing capacity of alveolospheres. Together, these data indicate a critical role for cellular bioenergetics and redox homeostasis in an organoid model of self-organization and support the concept of thermodynamic entropy in aging biology.

Flexible and Proficient Routing in Mobile Ad-Hoc Network

Mobile ad hoc networks (MANETs) can be distinct as a gathering amount of mobile nodes which build the irregular network lacking assist of several network structures. Every mobile node entering together as host as well as a router also it transmits the data to receiver via multi-hop manner. The dynamic topography, node mobility, offers large amount of degree, self-organizing capacity that is a features of MANET. Owing to the scenery intends to improvement of routing is disputing chore for communication surroundings. In this paper, Flexible and Proficient Routing in MANET is introduced. Here, Routing can be achieved both multi-hop and multipath. In an efficient network, care to achieve both in the most optimized manner is essential. An analysis of when multi-hop data transmission is required and when multipath transmission can be used for efficient communication is analyzed. The results provide better routing efficiency in MANET.

Environmental Movements in Turkey from the Perspective of Commons

Enclosure movements on the commons continue without slowing down especially in the developing countries; as well as the grassroots movements against them. Enclosure movements frequently contain several foreign investments and appropriations to other uses on natural resources and/or assets such as pastures, forests, rivers, agricultural lands and seashores, which are crucial income sources for rural households. Among other factors, the shortcomings in the common management processes, cooperatives and self-organization capacity of the stakeholders ease the enclosure process. The study investigates the environmental movements as commoning practices in Turkey in the last decade through media analysis and surveys. The study aims to constitute a scientific basis to enhance the EIA processes in order to encourage proactive responses to environmental crises, prevent enclosure movements on the commons and maintain the local sustainable development. Hence, firstly the environmental movements in Turkey are spatialized by GIS analyst tools to constitute an environmental inventory including time, types, frequency and location of the movements; secondly, an online survey is conducted with the environmental NGOs; thirdly, two possible scenarios are suggested through a triple-scale scoring system; and finally, several recommendations are proposed in order to sustain the commons.

Novel synthetic biology approaches for developmental systems.

Recently, developmental systems are investigated with increasing technological power. Still, open questions remain, especially concerning self-organization capacity and its control. Here, we present three areas where synthetic biology tools are used in top-down and bottom-up approaches for studying and constructing developmental systems. First, we discuss how synthetic biology tools can improve stem cell-based organoid models. Second, we discuss recent studies employing user-defined perturbations to study embryonic patterning in model species. Third, we present “toy models” of patterning and morphogenesis using synthetic genetic circuits in non-developmental systems. Finally, we discuss how these tools and approaches can specifically benefit the field of embryo models.

Autophosphorylation-induced self-assembly and STIL-dependent reinforcement underlie Plk4’s ring-to-dot localization conversion around a human centriole

ABSTRACT Polo-like kinase 4 (Plk4) is a key regulator of centriole biogenesis. Studies have shown that Plk4 undergoes dynamic relocalization from a ring-like pattern around a centriole to a dot-like morphology at the procentriole assembly site and this event is central for inducing centriole biogenesis. However, the detailed mechanisms underlying Plk4’s capacity to drive its symmetry-breaking ring-to-dot relocalization remain largely unknown. Here, we showed that Plk4 self-initiates this process in an autophosphorylation–dependent manner and that STIL, its downstream target, is not required for this event. Time-dependent analyses with mEOS-fused photoconvertible Plk4 revealed that a portion of ring-state Plk4 acquires a capacity, presumably through autophosphorylation, to linger around a centriole, ultimately generating a dot-state morphology. Interestingly, Plk4 WT, but not its catalytically inactive mutant, showed the ability to form a nanoscale spherical assembly in the cytosol of human cells or heterologous E. coli, demonstrating its autophosphorylation-dependent self-organizing capacity. At the biochemical level, Plk4 – unlike its N-terminal βTrCP degron motif – robustly autophosphorylated the PC3 SSTT motif within its C-terminal cryptic polo-box, an event critical for inducing its physical clustering. Additional in vivo experiments showed that although STIL was not required for Plk4’s initial ring-to-dot conversion, coexpressed STIL greatly enhanced Plk4’s ability to generate a spherical condensate and recruit Sas6, a major component of the centriolar cartwheel structure. We propose that Plk4’s autophosphorylation-induced clustering is sufficient to induce its ring-to-dot localization conversion and that subsequently recruited STIL potentiates this process to generate a procentriole assembly body critical for Plk4-dependent centriole biogenesis.

Factors Influencing the Development of Rural Producer Organizations in Post-War Settings. The Case of Coffee Growers Associations in Southern Tolima, Colombia

Since civil wars hit rural areas intensely, Rural Producer Organizations (RPO) -as forms of long-term collective action or cooperation among small farmers- are considered essential for peacebuilding. However, the factors underpinning the formation and performance of RPO post-war are unclear. Based on a case study in the municipality of Planadas, Colombia, where the former communist guerrilla Revolutionary Armed Forces of Colombia – People’s Army – FARC-EP was formed and several associations flourished post-war, this article identifies 14 contextual factors facilitating the rise of RPO. Contrasting the findings with variables identified by collective action, commons theory, and literature on RPO, it was determined that four additional contextual variables play a critical role in RPO development post-war, namely, legacies of war, resilience strategies, institutional intermediaries, and discourses. Legacies of war refer to the vestiges left by the kind of relationship developed between the main armed actor and the civilians in wartime. Economic activity as a resilience strategy indicates civilians’ strategies to stay aside from the confrontation, reducing the probability of being harmed and preventing their involvement in the war or illegal economic activities. Intermediary institutions are third-party organizations that influence RPO. In the case considered, this role was developed by certification schemes known as Voluntary Sustainability Standards. Controverting critical literature on the effects of the standards, the results suggest that they can enhance self-organizing capacities post-conflict at the local level. Finally, discourses refer to additional incentives for RPO development regarding what participants consider valuable beyond economic benefits. Consequently, the article presents the foundations of an expanded framework to understand and foster RPO growth in post-war settings.

Mechanical Tension Promotes Formation of Gastrulation-like Nodes and Patterns Mesoderm Specification in Human Embryonic Stem Cells.

Embryogenesis is directed by morphogens that induce differentiation within a defined tissue geometry. Tissue organization is mediated by cell-cell and cell-extracellular matrix (ECM) adhesions and is modulated by cell tension and tissue-level forces. Whether cell tension regulates development by modifying morphogen signaling is less clear. Human embryonic stem cells (hESCs) exhibit an intrinsic capacity for self-organization, which motivates their use as a tractable model of early human embryogenesis. We engineered patterned substrates that recapitulate the biophysical properties of the early embryo and mediate the self-organization of "gastrulation-like" nodes in cultured hESCs. Tissue geometries that generated local nodes of high cell-adhesion tension directed the spatial patterning of the BMP4-dependent "gastrulation-like" phenotype by enhancing phosphorylation and junctional release of β-catenin to promote Wnt signaling and mesoderm specification. Furthermore, direct force application via mechanical stretching promoted BMP-dependent mesoderm specification, confirming that tissue-level forces can directly regulate cell fate specification in early human development.

Influence of the imagined community on the ‘correctness’ of social work in social-legal protection of children in the Czechia

ABSTRACT The imagined community of social workers provides a collective resemblance to the individual construction of the correctness of performance of social work. This paper aims to understand the influence of the imagined community and its shared social worker’s metanarrative on the ‘correctness’ of social work in social and legal protection of children in Czechia. The data were created through a qualitative research strategy, more specifically through sets of three repeated interviews with eight well-experienced social workers from the office of social and legal protection of children. The data were analyzed using the constructivist grounded theory by Charmaz. The research conceived six roles of the imagined community that fulfill specific functions, plus two functions for which the informants did not create any specific roles of imagined community; making these functions in the informants’ view unfulfilled. Specifically, this concerned self-organizing capacity as well as activation and protection of a social worker. The generated data are further discussed in the context of education and training designed for social workers.

Young People's Autonomy and Psychological Well-Being in the Transition to Adulthood: A Pathway Analysis.

Young people transition to adulthood via diverse pathways; among the most significant are those dominated by education, employment, or social disadvantage. These pathways are determined, to a large extent, by the level of well-being and autonomy young people develop to help them face their own realities. The aim of this study is to analyze the relationship between young people’s psychological well-being and autonomy – key factors in the transition to adulthood – and the relationship these factors have with the main pathways followed during transition. To this end, Ryff’s Model of Psychological Well-being and the Transition to Adulthood Autonomy Scale (EDATVA) were used to evaluate a total of 1148 Spanish and Colombian subjects aged between 16 and 21. Correlations and differences between scores were subsequently analyzed. Subjects were also asked to identify the most relevant aspect of their transition to adulthood as either education, employment, or social disadvantage. Results from all three pathways for transition to adulthood show a trend in which higher levels of well-being correspond to higher levels of autonomy. In general, the results for the young people on the education pathway show high levels of autonomy and well-being, as well as a significantly higher level of critical thinking compared to young people on other pathways. The scores from employed young people reveal a greater capacity for self-organization in relation to the other two pathways. The results for disadvantaged young people show significantly greater socio-political engagement than that of young people on the education and employment pathways. However, the disadvantaged group also displays the lowest level of psychological well-being. These results provide elements for a better understanding of young people’s different transition pathways to adulthood and constitute an important point of reference for future research. They also provide data that may be relevant in guiding potential educational, psychological, and social interventions within this population group.