Event Abstract Back to Event Corrosion and cell behaviour of phosphate mineral coating on magnesium alloy Bin Jiang1, Lilan Zeng1, Ying Zhao1 and Kelvin Wk Yeung2 1 Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Center for Human Tissues and Organs Degeneration, China 2 The University of Hong Kong, Department of Orthopaedics & Traumatology, Hong Kong, SAR China Introduction: Magnesium-based metals have attracted much attention in recent years due to their potential applications on cardiovascular stents and bone implants. However, their inadequate corrosion resistance in a physiological environment becomes the major obstacle limiting wider applications. A number of surface treatments have been adopted to enhance corrosion resistance. However, most surface coatings only provide a barrier against corrosive species but not tailor the biocompatibility. In this study, biocompatible phosphate mineral coatings are synthesized using hydrothermal treatment on WE43 magnesium alloy surfaces and their corrosion resistance and cytocompatibility are examined systematically. Materials and Methods: As cast WE43 Mg alloys were treated in K2HPO4 solution at 200°C for 1 to 3h, respectively. X-ray diffraction (XRD) with Cu Ka radiation and scanning electron microscopy (SEM) were conducted to characterize the phase composition and microstructure. The electrochemical impedance spectroscopy (EIS) of the samples in phosphate buffered saline at 37°C was measured on an electrochemical workstation (CHI660E). The cytoskeleton and cell nuclei were observed by fluorescence microscopy. Results and Discussion: Fig. 1a shows that the sample surfaces are partially covered with petal-like-shaped products after 1 h of hydrothermal treatment. With the increase of the treated time, an intact and dense coating with petal-like-shaped structure is formed on the surface of samples (Fig. 1b-c). The XRD patterns (Fig.1d) show the coatings are mainly composed of Mg(OH)2 and KMgPO4·6H2O. After 5 h of culture, most of the MC3T3-E1 cells on the untreated WE43 alloys fail to spread well (Fig.1e). In contrast, the 3 h-treated sample shows obvious filopodia and flattened membranes (Fig.1f), suggesting that the phosphate mineral coating with better cytocompatibility. The Nyqusit plot (Fig.2a) exhibits that the diameter of the capacitive arcs is increased with the extension of the hydrothermal treated time. And the Bode diagram (Fig.2b) shows similar enhancing trend. The 3 h-treated sample exhibits the largest diameter and the biggest impedance in the Nyquist plot and Bode diagram as compared to the other samples, suggesting the best corrosion resistance due to the protective screen of compact phosphate mineral coatings. Proper reduction of the degradation rate creates a relatively stable interface for cell growth, thus leading to improved cytocompatibility of WE43 magnesium alloys. Conclusions: Phosphate mineral coatings with petal-like-shaped structure are formed on the WE43 Mg alloys by hydrothermal treatment. The coatings are mainly composed of Mg(OH)2 and KMgPO4·6H2O, which effectively improve the corrosion resistance of WE43 magnesium alloys. At the same time, proper reduction of the degradation rate creates a relatively stable interface for cell growth, leading to improved cytocompatibility. Science and Technology Department of Guangdong Province (2014A010105033); Shenzhen overseas high level talents of innovation and Entrepreneurship (110811003586331,KQCX20140521115045444); Guangdong Natural Science Foundation Doctoral project (2014A030310129)