The present study carried out in Al-Diwaniya city, 71clinical specimens were taken from the patients withdermatophytosis. The age of patients were between (1-50) years, real positive cultural examinations were49(69%), whereas negative cultural examinations were 22 (31 %) . The occurrence of dermatophyte fungiwere higher in females than males, depending on the gender, as for the age groups, the infection was higherin the age group 31-40 and 41-50 years followed by the age group ? 11 years, whereas the lowest infectionwas among the age group 21-30 and 12-20 years. Also, the result of this study showed that 37(52.11%)patients were housewives, 20 (28.16%) students, 7(9.85%) office employees, 7(9.85%) hospital workers.Out of 49 patients 25(51.0 %) cases were Tinea pedis, followed by Tinea cruris15(30.6%), Tinea capitis5(10.2%), and 4(8.2%) Tinea unguium. The result of this study also showed that 31(63.3%) isolates wereT. rubrum, 11( 22.4%) T. mentagrophytes , E. floccosum and M. canis isolates were found 2(4.1%) and5(10.2%) respectively