Event Abstract Back to Event Stock boundaries of Mullus barbatus (Mullidae) and Parapenaeus longirostris (Penaeidae) populations in the Italian seas: development of genomic 2b-RAD markers Alice Ferrari1* 1 University of Bologna, BiGeA, Italy Over-fishing is one of the most complex and argued issue among the human impacts affecting the marine environment. The development of integrated policies for fish stock conservation and its responsible use are essential. Nowadays, genomics is considered an ultimate tool to define stock boundaries and population structure as it expands the focus on the entire organism’s genetic makeup and its interaction with the environment. An example of effort and capitalization on genomics is shown by the Italian RITMARE Flagship Project, which aims to create a genomic mapping for stocks of two commercially important species, the red mullet (Mullus barbatus L.) and the deep-water rose shrimp (Parapenaeus longirostris Lucas, 1847), through the use of 2b-RAD technology, based on sequencing the uniform fragments produced by type IIB restriction endonucleases. A sampling design covering 8 Geographical Sub-Areas was defined on the basis of nursery areas, abundance and importance in local fishery and it was completed during the MEDITS 2013 survey. After the extraction of high quality genomic DNA from 7 individuals for each species, the 2b-RAD protocol was tested for the first time. Thereafter, a high-throughput sequencing of libraries was accomplished through Illumina® platform and the software STACKS was used to analyze the sequences. Several millions of high quality reads per individual were obtained and approximately from 1.0 to 2.0x10e3 candidate SNP markers were identified. These preliminary results confirm the successful application of 2b-RAD technology on both non model species and, above all, its effective extendibility to larger number of individuals and population samples, necessary to assess stock boundaries and stock units in the Italian seas. Acknowledgements Francesco Maroso2, Anna Orpelli1, Stefano Rambaldi1 and Alessia Cariani1 1BiGeA Department– UNIVERSITY OF BOLOGNA 2BCA Department– UNIVERSITY OF PADOVA Keywords: Red mullet, deep-water rose shrimp, Genomics, 2b-RAD, SNPS markers Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: FISHERIES AND MANAGEMENT Citation: Ferrari A (2014). Stock boundaries of Mullus barbatus (Mullidae) and Parapenaeus longirostris (Penaeidae) populations in the Italian seas: development of genomic 2b-RAD markers. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00157 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Apr 2014; Published Online: 18 Jul 2014. * Correspondence: Dr. Alice Ferrari, University of Bologna, BiGeA, Ravenna, 48123, Italy, alice.ferrari6@unibo.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Alice Ferrari Google Alice Ferrari Google Scholar Alice Ferrari PubMed Alice Ferrari Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.