The maxillary first molar is crucial for proper bite formation and jaw positioning in adulthood. The prevalence of dental caries in the study's sample population suggests it is a common candidate for endodontic therapy. Multiple studies have shown that the canal and root morphology of the maxillary first molar are abnormal. The distobuccal and palatal roots of 286 maxillary first molars were removed at the furcation. The mesiobuccal roots were then imaged using cone-beam computed tomography (CBCT) on all specimens. The specimens were analyzed in comparison with one another. The following factors were studied. The number and arrangement of canals, as described by Vertucci; the presence or absence of an isthmus, as described by Kim; the canal curvature angle, as measured by the Schneider method; characteristics such as calcified segments, lateral canals, and an apical delta.For the flow analysis, CBCT was utilized to examine the root channel architecture of 286 mesiobuccal (MB) permanent maxillary first molar. Type I canal arrangement was found in 51.6% of teeth, Type II in 33.3%, Type III in 4.6%, Type IV in 4.2%, Type V in 2.5%, and Type VII in 1.5%. Type I, II, III, and V isthmus were each present in 26%, 6.7%, 9.5%, and 10.2% of the samples, respectively. Teeth with MB1 canal angulations of 0-20 degrees, 21-40 degrees, and more than 40 degrees were found in 56, 188, and 41 teeth, respectively. From 0 to 20 degrees, 21 to 40 degrees, and more than 40 degrees, 15 teeth, 88 teeth, and 25 teeth, respectively, had angulations in their MB2 canals. Only 9.8% of the samples had lateral canals, while 16.1% had both accessory canals and apical deltas. There were 19 cases with calcified segments in the coronal third of the MB1 canal and 13 cases in the middle third. There were no calcifications at the distal end of the MB1 canal. Thirteen of the specimens showed calcification only in the most caudal third of the MB2 canal, whereas the middle and distal thirds were uncalcified. Utilizing a noninvasive approach, a CBCT scan has the capacity to provide valuable insights into the root canal configuration.