Canadian Society Of Clinical Chemists Research Articles

Canadian society of clinical chemists position paper: standardization of selected polypeptide hormone measurements

This Canadian Society of Clinical Chemists (CSCC) sponsored position statement addresses the problem of nonuniformity of standardization of polypeptide hormone analyses. First we review the structural diversity of selected polypeptide hormones including growth hormone (GH), prolactin, follitropin (FSH), lutropin (LH), human chorionic gonadotropin (hCG), and thyrotropin (TSH) and the problem that this heterogeneity creates. The history and availability of reference materials for the analyses of these hormones are then summarized. Finally we make a list of recommendations regarding standardization, methods of measurement, and reporting of polypeptide hormone results. Implementation of the recommendations will lead to improvement in inter-laboratory comparability and more useful hormone assays.

Specific method for serum creatinine determination based on ion exchange chromatography and an automated alkaline picrate reaction — A proposed reference method

A proposed reference method for serum creatinine has been developed under the auspices of the Committee on Reference Methods and Reference Materials of the Canadian Society of Clinical Chemists. A serum ultrafiltrate at pH 2.0 is applied through a closed sample loop injection system to a short column containing cationic resin of high resolving power. Elution with sodium citrate buffer by means of minipump at constant rate passes the eluate into alkaline picrate reagents in a continuous flow system (Technicon AAIII pump, AAII colorimeter 50 mm x 1.5 mm flow cell, narrow band width filter). The colour reaction peak is monitored visually to verify specificity and the area is calculated electronically. Specificity has been demonstrated by use of Jaffé-reactive substances such as glucose, sodium acetoacetate, L-ascorbic acid, pyruvic acid, L-dopa and glycocyamidine and also by use of an alternate colour reaction, sodium 3,5-dinitrobenzoate in place of alkaline picrate in the analysis of serum pools. Routine methods in common use, i.e., manual and automated alkaline picrate procedures, demonstrated a statistically significant high bias in interlaboratory studies in which this procedure was used for reference.

23rd National Meeting of the American Association of Clinical Chemists, and 15th Annual Meeting of the Canadian Society of Clinical Chemists

Journal Article 23rd National Meeting of the American Association of Clinical Chemists, and 15th Annual Meeting of the Canadian Society of Clinical Chemists Get access Clinical Chemistry, Volume 17, Issue 6, 1 June 1971, Pages 554–562, https://doi.org/10.1093/clinchem/17.6.554 Published: 01 June 1971

A survey of methods and instrumentation in use in the laboratories of members of the Canadian society of clinical chemists across Canada

Data have been presented from a survey conducted among members of the Canadian Society of Clinical Chemists on the most commonly performed tests, the methods used for these determinations and the instrumentation. Comparison of the data with earlier surveys emphasizes the continuing trend toward automation and the application of new techniques to clinical chemistry, e.g. fluorometry, osmometry and atomic absorption spectrophotometry. There is evidence of the use of commercially produced diagnostic kits particularly for enzyme determinations. A considerable variability exists among laboratories in the figures quoted as normal ranges of values for the various constituents. The need for each laboratory to re-establish their own ranges is emphasized.

CANADIAN SOCIETY OF CLINICAL CHEMISTS

Journal Article CANADIAN SOCIETY OF CLINICAL CHEMISTS Get access Clinical Chemistry, Volume 4, Issue 4, 1 August 1958, Pages 339–345, https://doi.org/10.1093/clinchem/4.4.339 Published: 01 August 1958