Control Campaigns Research Articles

Harnessing the Power of Testimonial Videos: A Deep Dive into the #1Minute2Quit Campaign for Spreading Awareness on the Health Hazards of Tobacco

In Indonesia, social media usage continues to grow, with 167 million users as of January 2023, 79.5% of whom are over 18 years old. Platforms like Instagram, Facebook, TikTok, and Twitter play key roles in public health campaigns. The #1Minute2Quit campaign, part of the #SuaraTanpaRokok movement, was launched in 2017 to raise awareness about the harmful effects of tobacco through 1-minute testimonial videos. By July 2023, the campaign had generated 21 testimonial videos from former smokers, with over 11,000 total engagements and 152,000 potential impressions. A qualitative content analysis was conducted on these videos to examine engagement patterns, messaging strategies, and audience responses. The analysis revealed that testimonial videos offering practical tips and encouragement to quit smoking attracted higher engagement, particularly among young adults (18-24 years), females, and individuals from higher socioeconomic backgrounds. Culturally tailored content also resonated more with diverse ethnic groups. The findings suggest that culturally sensitive and targeted messaging, combined with practical advice, is key to enhancing the effectiveness of social media health interventions. This study underscores the importance of ongoing engagement strategies in tobacco control campaigns and offers recommendations for refining future health messaging to address diverse demographic groups. Further research should explore the long-term impact of content variations across different social media platforms. Keywords: Testimonial videos, tobacco control, social media campaign, #1Minute2Quit, public health campaign.

A cross-sectional study on exposure to secondhand smoke in indoor public places and attitudes of residents towards the smoke control ordinance in public places.

Secondhand smoke (SHS) exposure is a serious public health problem. This study aims to collect data on tobacco control since the implementation of the new version of the Smoke Control Ordinance in Public Places of Hangzhou (the Ordinance), combined with questionnaire surveys to understand the SHS exposure situation in public places, relevant knowledge and attitudes of residents in Hangzhou. We used data from a population-based tobacco control survey of 2746 adults aged 15-75 years conducted in Hangzhou, Zhejiang Province in March 2023. Data were collected using a self-administered questionnaire consisting of demographic characteristics, exposure to tobacco, awareness of tobacco hazards, knowledge of the Ordinance, and behaviors and attitudes towards tobacco control in public places. Chi-squared tests were performed to examine the differences in knowledge and attitudes related to tobacco control in indoor public places among residents with different characteristics in Hangzhou. Multivariable logistic regression was used to identify variables affecting satisfaction with the effectiveness of tobacco control in public places. Of the 2155 non-smokers, 1006 (46.68%) had been exposed to SHS in public places. More than 89.00% of participants supported the Ordinance. Satisfaction with effectiveness of tobacco control in public places in Hangzhou was 68.54%, and the subcomponents that influenced it were satisfaction with tobacco control publicity campaigns (AOR=1.85; 95% CI: 1.19-2.88), satisfaction with tobacco control surveillance and enforcement (AOR=3.91; 95% CI: 2.43-6.30), satisfaction with the smoke-free demonstration for government departments (AOR=5.79; 95% CI: 3.96-8.47), and satisfaction with individual tobacco control behavior (AOR=11.68; 95% CI: 8.53-15.99). It is necessary to strengthen tobacco control publicity to increase the participation of residents and to gain a deeper understanding of the subjective willingness and needs of residents to participate in tobacco control campaigns in order to improve residents' individual satisfaction.

The impact of culturally-informed messages to reduce sugar-sweetened beverage consumption: An experiment among Black women in the United States.

Sugar-sweetened beverage (i.e., sugary drink) consumption is associated with chronic health issues that disproportionately affect Black women. Culturally-informed (CI) health campaigns may be more effective among Black women than campaigns designed for general audiences. This study assesses the effects of a CI campaign on consumption intentions, comparing these effects to general audience and control campaigns. We conducted an online between-persons randomized experiment with a national convenience sample of 502 Black women in February 2023. Participants were randomly assigned to view a CI, general audience, or control campaign. Outcomes were intentions to decrease sugary drink consumption (primary outcome; range 1-7), knowledge of (range 0-4) and perceived susceptibility to health harms (range 1-5) and sharing intentions (range 0-3). The CI campaign had significantly higher perceived cultural relevance (M = 4.61) than the general audience (M = 3.64) or control (M = 3.66; p's<0.05) campaigns. Consumption intentions did not differ by campaign condition (F = 0.03, p>0.05). There was no main effect of campaign condition on knowledge or perceived susceptibility (p's>0.05), though findings were moderated by body size. Based on body size, women reported less perceived susceptibility or knowledge when exposed to the CI campaign, compared to either the control or general audience campaign. Sharing intentions did not differ by campaign condition. Future research should continue to examine the role of refining message content over a longer duration to understand whether the anticipated impact of CI messages can be achieved in the context of sugary drink consumption among Black women.

Stray Cat Colonies Lacking Health Surveillance and Management Pose Infection Pressure for Aelurostrongylus abstrusus on Sympatric Domestic and Wild Felids.

The prevalence of respiratory nematodes in domestic animals has increased in Europe in recent decades. Aelurostrongylus abstrusus is the most common parasitic nematode of the respiratory tract in felids, and an increase in its prevalence has been suggested in different European countries including Spain, with values ranging from 0% to 30%. The Baermann technique was used to detect metastrongyloid larvae in 93 faecal samples from cats living in colonies (n = 29), cats living in shelters (n = 30), and owned cats (n = 34). Eleven cats (11.2%) were infected by A. abstrusus, the only species detected. The prevalence in colony cats (34.5%, 10/29) was significantly higher than the 3.3% (1/30) prevalence in shelter cats. Conversely, A. abstrusus was not found in any of the owned cats. The results can be representative of other areas in the Mediterranean basin, indicating the need to improve feline management and knowledge about the distribution and abundance of this parasite through geographically wider studies in Spain. This study emphasizes the importance of deworming domestic cats, especially those from street and rural environments or with outdoor lifestyles. The results also urge the need for population and health control of street cat colonies. Incorporating a deworming protocol in the current trapping-neutering-release campaigns for cat population control will be essential.

Peadiatric amputation from traditional bone setters’ gangrene in Ibadan: a malady

Background: Pediatric amputations resulting from gangrene pose a significant medical challenge, especially in resource-limited settings. In Nigeria, traditional bone setters (TBS) often serve as the first point of contact for musculoskeletal injuries, but their practices can lead to severe complications, including gangrene. Our hospital manages many of such cases. This study aims to explore the epidemiology, clinical presentation, and outcomes of pediatric patients who developed gangrene requiring amputation following TBS treatment, with the goal of informing public health strategies and improving pediatric care quality. Methods: The study is a retrospective study which seek to extract the data from the hospital record all the patient who underwent amputation due to gangrene after initial TBS treatment. All the case notes were retrieved and data was extracted and analyzed using descriptive statistics. The study adhered to ethical standards and anonymized data to protect patient confidentiality, ensuring the study's integrity. Results: The study involved 33 pediatric patients aged 8.3 years, with a significant gender disparity. Most patients were from the Yoruba ethnic group and practiced Islam. 87.9% of parents had no formal education. Gangrene was most common in the left hand, and falls were the leading cause of initial injury. The average surgery time was 81.2 minutes, with a 13-day hospital stay. Post-operative complications occurred in 43.5% of patients, with surgical site infections (SSI) being the most common. Only 8.7% received occupational therapy. Conclusions: The study highlights post-operative care challenges in pediatric amputations, including high complications and limited access to services. It suggests targeted interventions, infection control, and public health campaigns to improve outcomes. Future research should include long-term follow-up studies.

UNDERSTANDING DAIRY SMALLHOLDERS’ INTENTION TO PARTICIPATE IN THE CONTROL AND ERADICATION OF BOVINE BRUCELLOSIS

&lt;p&gt;&lt;strong&gt;Background:&lt;/strong&gt; Brucellosis (&lt;em&gt;Brucella abortus&lt;/em&gt;) is a disease that affects cattle and has a significant economic impact on the livelihood of farmers in developing countries. &lt;strong&gt;Objective:&lt;/strong&gt; The research aimed to understand the dairy smallholders’ intention to participate in the national campaign to control and eradicate bovine brucellosis in Aculco, State of Mexico. &lt;strong&gt;Methodology:&lt;/strong&gt; The study was conducted with 196 farmers who were already engaged in the campaign. The reasoned action approach (RAA) was used as a theoretical framework, and Spearman rank-order correlations were used to analyze the data. &lt;strong&gt;Results:&lt;/strong&gt; The results showed that farmers firmly intended to participate in the campaign in the next 12 months. The intention was associated with farmers’ years of experience as milk producers and perception of the importance and usefulness of the campaign in addition to farm characteristics. Farmers’ intention was also related to positive beliefs (drivers), such as participation in the campaign, preventing the disease in the herd, enabling access to government support and subsidies, and avoiding spreading the disease to people. Other social referents such as veterinarians, farmers, and farmers’ sons also played an important role in farmers’ intentions. A couple of significant constraints were identified: the lack of financial resources, communication between farmers and personnel in charge of the campaign. &lt;strong&gt;Implications: &lt;/strong&gt;The study provided insights to improve extension activities towards small-scale dairy farmers to participate in the national campaign of control and eradication of bovine brucellosis.&lt;strong&gt; &lt;/strong&gt;&lt;strong&gt;Conclusion:&lt;/strong&gt; the three components of the RAA (attitude, perceived norms, and perceived behavioral control) were strong predictors of farmers’ intention to participate in the brucellosis eradication campaign. They enabled the factors influencing farmers’ intentions to be explored more deeply.&lt;/p&gt;

Long-Lasting Insecticidal Net Campaigns for Malaria Control Considering Prioritization and Equity

Malaria remains a significant public health concern in several developing countries. Tropical weather, coupled with poor environmental and socioeconomic conditions, increases mosquito activity and disease transmission in impoverished nations. The most effective strategy to prevent and control malaria is the use of long-lasting insecticidal nets, or LLINs. Ensuring that LLINs are effectively and equitably distributed to those who need them most is crucial yet challenging, especially since financial and health resources are scarce in endemic countries. Through a real-world case study conducted in the Brazilian Amazon, which accounts for 99% of malaria cases in Brazil, we propose a Malaria Vulnerability Index (MVI). This composite index encompasses epidemiological, socioeconomic, and environmental factors. Using the MVI, we developed a prioritization-based location-allocation model to maximize the benefits of targeting the most vulnerable municipalities for malaria interventions. Our mathematical model relies on discrete coverage levels, which makes the allocation of LLINs more flexible while ensuring community-wide protection. We also explore the theoretical relationship between our proposed model and classical demand covering problems. Finally, we discuss some approaches to delivering more equitable solutions by minimizing the number of underserved areas. Our results include comparisons between various malaria interventions and provide practical insights. In particular, we show that we can drastically reduce the number of underserved areas without compromising the effectiveness of the LLINs allocation through lexicographic optimization. The results also reveal that with investment levels up to 50% of the ideal, we can fully protect the endemic area after 2 years of successive interventions.

Becoming hand hygiene heroes: Implementation of an infection prevention and control campaign for patient and family hospital safety

The COVID-19 pandemic highlighted that hand hygiene (HH) is key in preventing health care-associated infections. Patients and families are understudied targets for infection prevention and control practices. Previous campaigns to change HH behavior have been effective, but often face systemic challenges with implementation. The "Hand Hygiene Heroes" educational campaign was developed to improve patient and family HH at 2 tertiary care pediatric and obstetrics hospitals. Its multiple phases included visual materials, tailored activities for 9 hospital units, and long-term evaluation during a 2-year period. HH rates among patients/families and health care workers were measured at baseline and throughout the campaign via direct observation. Overall, HH significantly increased for both patients/families (OR 1.82, P .041) and health care workers (OR 2.15, P<.001) after campaign initiation. However, individual units had varying degrees of sustainment on follow-up evaluations. Duration of intervention, activity simplicity, active participation, and resource availability may affect success of campaign initiatives. Positive prognostic factors included mixed leadership support from administration and frontline workers, predetermined sustainability plans, and tailored activities by target audience. Implementation of hospital educational campaigns can be resource-intensive but can positively impact patient and family HH.

Optimal Allocation of Multi-Type Vaccines in a Two-Dose Vaccination Campaign for Epidemic Control: A Case Study of COVID-19

As a typical case of the optimal planning for the provision of restricted medical resources, widespread vaccination is considered an effective and sustainable way to prevent and control large-scale novel coronavirus disease 2019 (COVID-19) outbreaks. However, an initial supply shortage of vaccines is inevitable because of the narrow production and logistical capacity. This work focuses on the multi-type vaccine resource allocation problem in a two-dose vaccination campaign under limited supply. To address this issue, we extended an age-stratified susceptible, exposed, infectious, and recovered (SEIR) epidemiological model to incorporate a two-dose vaccination campaign involving multiple vaccine types to fully characterize the various stages of infection and vaccination. Afterward, we integrated the proposed epidemiological model into a nonlinear programming (NLP) model to determine the optimal allocation strategy under supply capacity and vaccine hesitancy constraints with the goal of minimizing the cumulative number of deaths due to the pandemic over the entire planning horizon. A case study based on real-world data from the initial mass vaccination campaign against COVID-19 in the Midlands, England, was taken to validate the applicability of our model. Then, we performed a comparative study to demonstrate the performance of the proposed method and conducted an extensive sensitivity analysis on critical model parameters. Our results indicate that prioritizing the allocation of vaccines to elderly persons is an effective strategy for reducing COVID-19-related fatalities. Furthermore, we found that vaccination alone will not be sufficient for epidemic control in the short term, and appropriate non-pharmacological interventions are still important for effective viral containment during the initial vaccine rollout. The results also showed that the relative efficacy of the first dose is a vital factor affecting the optimal interval between doses. It is always best to complete the two-dose vaccination schedule as soon as possible when the relative efficacy of the first dose is low. Conversely, delaying the second dose of a vaccine as long as possible to increase the proportion of the population vaccinated with a single dose tends to be more favorable when the relative efficacy of the first dose is high. Finally, our proposed model is general and easily extendable to the study of other infectious disease outbreaks and provides important implications for public health authorities seeking to develop effective vaccine allocation strategies for tackling possible future pandemics.

Prevalence of Strongyloides stercoralis and other helminths in four districts of Madagascar

BackgroundEstimation of prevalence of Strongyloides stercoralis infection is required in endemic areas, in order to identify areas in need of control programmes. Data on prevalence of strongyloidiasis in Madagascar are scant. Aim of this work was to estimate prevalence of S. stercoralis in four districts of Madagascar.MethodsFecal and serum samples collected in the context of a previous study on schistosomiasis were tested with S. stercoralis real-time PCR and serology, respectively. A multiplex real-time PCR for Ascaris lumbricoides, Ancylostoma duodenalis, Necator americanus, and Trichuris trichiura was done on fecal samples collected in the areas demonstrating higher prevalence of strongyloidiasis. Comparisons between proportions were made using Fisher exact test, with false discovery rate correction used for post-hoc comparisons. A multivariable Firth logistic regression model was used to assess potential risk factors for S. stercoralis infection.ResultsOverall, 1775 serum samples were tested, of which 102 of 487 (20.9%) and 104 of 296 (35.2%) were serological-positive in Marovoay and in Vatomandry districts (both coastal areas), respectively, compared to 28 of 496 (5.6%) and 30 of 496 (6.1%) in Tsiroanomandidy and in Ambositra districts (both highlands), respectively (adj. p < 0.001). PCR for S. stercoralis was positive in 15 of 210 (7.1%) and in 11 of 296 (3.7%) samples from Marovoay from Vatomandry, respectively, while was negative for all samples tested in the other two districts. High prevalence of A. lumbricoides (45.9%), hookworm (44.6%) and T. trichiura (32.1%) was found in Vatomandry. In the multivariable analysis, strongyloidiasis was associated with hookworm infection. Hookworm infection was also associated with male sex and lower education level.ConclusionsS. stercoralis prevalence proved higher in coastal areas compared to highlands. Different climatic conditions may explain this distribution, along with previous rounds of anthelminthics distributed in the country, which may have reduced the parasite load in the population. The high prevalence of the other soil-transmitted helminths (STH) in Vatomandry was unexpected, given the good coverage with benzimidazole in control campaigns. Further studies are needed to explore the risk factors for STH and S. stercoralis infections in Madagascar, in order to align with the WHO recommendations.

Rabies and the Arctic Fox (Vulpes lagopus): A Review.

The Arctic fox (Vulpes lagopus) is the primary infection reservoir of Arctic rabies, the dynamics of which are poorly understood and subject to significant spatiotemporal variation. Although rabies presence has been documented in the region since the mid-19th century, there is currently no evidence of rabies impacting Arctic fox population size. Under the influence of climate change in a rapidly evolving Arctic ecosystem, alterations in transmission dynamics are predicted, with implications for this species. Concurrently, the World Health Organization leads the United Against Rabies collective in the aim of elimination of dog-mediated rabies by 2030, and although efforts have justifiably been directed to tropical regions, elimination will require a good understanding of rabies in the Arctic. Therefore, this review aimed to provide an overview of current Arctic rabies understanding, while identifying the key knowledge gaps. The review covered spatiotemporal trends in rabies populations, population dynamics of the host species, and current theories about Arctic rabies persistence. It is still unclear how Arctic rabies can persist under low host densities, which has led to several hypotheses in recent years. Creation of high animal density "hotspots" caused by heterogenic fox distribution and multispecies congregations in response to food availability, extensive Arctic fox migration patterns, and the potential evolution to a less lethal variant of rabies may all be part of the explanation. Evidence for these theories by using recent genetic and modeling studies was evaluated within the review. There is currently insufficient evidence about the efficacy and feasibility of vaccines against Arctic rabies. Key knowledge gaps need addressing to enable future control campaigns.

Assessment of sublethal effects of permethrin on adult life characteristics and resistance dynamics in Aedes albopictus (Diptera: Culicidae)

Mosquitoes are regularly exposed to adverse effects of insecticides employed in field during vector control campaigns. Its primary goal is to eliminate the vector population; nevertheless, this practise typically ignores the residual impacts and long-term repercussions on the remaining population. Here, the current study analysed how sublethal exposure of insecticides alter the life qualities, genotypic and biochemical characteristics of mosquitoes. The resistance ratio value in Laboratory Resistant (Lab-R) larvae increased 10 times (0.010 mg/l to 0.108 mg/l) compared to Laboratory Susceptible (LabS) larvae. It also revealed that the surviving mosquitoes had 50% reduction in hatchability but had longer larval and pupal periods (15 days and 2 days), respectively. The survival rates decrease in female by 2 days but increase in male by 7 days which is of concern and necessitates additional study. Moreover, major role of monooxygenase was confirmed behind resistance development which was further supported by piperonyl butoxide assay where reduction in Tolerance Ratio (TR50) by 12-fold occurred and gene expression profile also showed high expression level of CYP6P12 gene. In resistant strain, cuticular thickness increased by 1.23 times and alteration at codon 1532 (ATC to TTC) on VGSC gene leads to mutation I1532F. The data gleaned from our work highlights the threat of sublethal insecticides on vector control techniques and offers ample evidence that the larval selection alters adult life qualities, metabolic properties and transgenerational features which contributes to the damage caused by resistance.

An Almost Empty Scoping Review: State of the Research on Tobacco Prevention and Cessation Messaging Strategies for Black and/or Latine LGBTQ+ Youth and Young Adults

ABSTRACT Research has yet to offer strong recommendations for effective tobacco prevention and cessation messaging that can reduce tobacco-related health disparities among Black and/or Latine LGBTQ+ youth and young adults. As a result of predatory marketing strategies and community stressors, among other factors, LGBTQ+ youth and young adults use tobacco products at higher rates than their non-LGBTQ+ peers. These disparities are uniquely complex among Black and/or Latine youth and young adults within the LGBTQ+ community, but there has been little research addressing the communication strategies that can promote tobacco prevention and cessation for these groups. Given the promise and history of successful health communication campaigns for tobacco control, this research is crucial. We thus conducted a scoping review to identify trends and gaps in the empirical research published from 2002–2022 that analyzed tobacco prevention and cessation communication strategies for Black and/or Latine LGBTQ+ youth and young adults (ages 12–30) living in the United States. Despite an initial search query of 3,182 articles after deleting duplicates, only five articles were eligible for inclusion, three of which evaluated the This Free Life campaign. Accordingly, we view our scoping review as an almost empty review. Although our results offer preliminary insight into messaging strategies used in these campaigns, our larger contribution is to expose the scarcity of tobacco-related communication research being conducted among Black and/or Latine LGBTQ+ communities. Given the marginalization these communities face, we issue a call to action for researchers and campaign designers and offer a series of suggestions for future research.

Locating Nesting Sites for Critically Endangered Galápagos Pink Land Iguanas (Conolophus marthae).

Invasive alien species control is recognized worldwide as a priority action to preserve global biodiversity. However, a lack of general life history knowledge for threatened species can impede the effectiveness of conservation actions. Galápagos pink land iguanas (Conolophus marthae) are endemic to Wolf Volcano, Galápagos, Ecuador. These iguanas are threatened by invasive alien species, particularly feral cats, that may affect their small population size. To guarantee the long-term survival of C. marthae, the Galápagos National Park Directorate is considering, along with an ongoing campaign of feral cat control, the implementation of a head-start program. However, the success of this management strategy necessarily relies on the identification of pink iguana nesting grounds, which were still unknown at the onset of this study. We modeled the movement patterns of male and female iguanas during the reproductive season, using location data collected from custom-made remote tracking devices installed on adult pink iguanas in April 2021. We first calculated for each individual the vector of distances from its starting location, which was defined as net displacement. We then used net displacement as the response variable in a generalized additive mixed model with day of the year as the predictor. Based on the hypothesis that males and females may behaviorally differ after mating, we looked for female-specific migratory behavior suggesting females were moving toward nesting areas. The results obtained confirmed our hypothesis, as females exhibited a distinct migratory behavior, reaching a small plateau area inside of Wolf Volcano's caldera and ca. 400 m below the volcano's northern rim. Moreover, once inside the caldera, females displayed a more aggregated distribution pattern. The movement data obtained allowed Galápagos National Park rangers to locate individual pink iguana nests and subsequently to sight and collect the first observed hatchlings of the species. This work constitutes a necessary baseline to perform dedicated studies of pink iguana nests and emerging hatchling iguanas, which is an essential step toward the development of an effective head-start program.

315b - Overlap of REACH/ OSH legislation: Inspection campaigns for control of exposure to substances subject to REACH restriction

315b - Overlap of REACH/ OSH legislation: Inspection campaigns for control of exposure to substances subject to REACH restriction

Assessing the impact of preventative measures to limit the spread of Toxoplasma gondii in wild carnivores of Madagascar.

Novel multihost pathogens can threaten endangered wildlife species, as well as humans and domestic animals. The zoonotic protozoan parasite Toxoplasma gondii is transmitted by members of Felidae and can infect a large number of animal species, including humans. This parasite can have significant health consequences for infected intermediate hosts and could further endanger wild carnivore populations of Madagascar. Building on an empirical characterization of the prevalence of the pathogen in local mammals, we used mathematical models of pathogen transmission in a multihost community to compare preventative measures that aim to limit the spread of this parasite in wild carnivores. Specifically, we examined the effect of hypothetical cat vaccination and population control campaigns on reducing the risk of infection by T. gondii in wild Eupleridae. Our model predicted that the prevalence of exposure to T. gondii in cats would be around 72% and that seroprevalence would reach 2% and 43% in rodents and wild carnivores, respectively. Reducing the rodent population in the landscape by half may only decrease the prevalence of T. gondii in carnivores by 10%. Similarly, cat vaccination and reducing the population of definitive hosts had limited impact on the prevalence of T. gondii in wild carnivorans of Madagascar. A significant reduction in prevalence would require extremely high vaccination, low turnover, or both in the cat population. Other potential control methods of T. gondii in endangered Eupleridae include targeted vaccination of wild animals but would require further investigation. Eliminating the threat entirely will be difficult because of the ubiquity of cats and the persistence of the parasite in the environment.

Endoscopically diagnosed hookworm infestation in an adult with chronic iron deficiency anaemia

Despite advances in hookworm control and prevention campaigns, the prevalence remains high in sub-Saharan Africa. Iron deficiency anaemia is a major complication in those with prolonged infection, a high hookworm burden, and undernutrition, though most infected people are asymptomatic. While infected patients are commonly diagnosed by the presence of ova or cysts in the stool, gastrointestinal (GI) endoscopy is required for those with negative stool tests and chronic iron deficiency anaemia. A 48-year-old female with symptoms of anaemia for nearly two years presented to Mbarara Regional Referral Hospital with worsening palpitations, easy fatigability, and dizziness over a period of one month. She was given blood transfusions on two occasions. She lives near the lake where she also gets water for drinking and domestic use. We advise GI endoscopy for all patients with chronic iron deficiency anaemia of unexplained aetiology on standard non-invasive testing. Hookworm’s mass empirical treatment policy in endemic areas should be further emphasised.

Occupancy dynamics of free ranging American mink (Neogale vison) in Greece.

Identifying the environmental factors that determine the occurrence of invasive species is essential in defining and implementing effective control campaigns. Here, we applied multi-season occupancy models to analyze American mink (Neogale vison) track data collected using 121 floating rafts, as a function of factors occurring at multiple spatial scales. Our overall aim was to identify those factors that determine the use, colonization or abandonment of rafts by free ranging individuals found in western Macedonia, Greece. We found that increasing values of shrubs and rock cover at the micro-habitat scale were positively associated with the species' probability of raft use, as was the density of medium-sized rivers at the landscape scale. Colonization was found to increase with increasing amounts of shrub and reed cover; however, both variables were not informative. Conversely, the distance from the nearest fur farm was highly informative in predicting raft abandonment by the species. Effective control actions may require removal by trapping along rocky or densely vegetated riverbanks or lake shores located in the vicinity of the established fur farms in the area. Habitat management, although possible, may be difficult to implement due to the ability of the species to adapt. Finally, fur farms should maximize security and establish an early warning and rapid eradication system in case of future escapes.

Modelled impact of Tiny Targets on the distribution and abundance of riverine tsetse.

The insecticide-treated baits known as Tiny Targets are one of the cheapest means of controlling riverine species of tsetse flies, the vectors of the trypanosomes that cause sleeping sickness in humans. Models of the efficacy of these targets deployed near rivers are potentially useful in planning control campaigns and highlighting the principles involved. To evaluate the potential of models, we produced a simple non-seasonal model of the births, deaths, mobility and aging of tsetse, and we programmed it to simulate the impact of seven years of target use against the tsetse, Glossina fuscipes fuscipes, in the riverine habitats of NW Uganda. Particular attention was given to demonstrating that the model could explain three matters of interest: (i) good control can be achieved despite the degradation of targets, (ii) local elimination of tsetse is impossible if invasion sources are not tackled, and (iii) with invasion and target degradation it is difficult to detect any effect of control on the age structure of the tsetse population. Despite its simplifications, the model can assist planning and teaching, but allowance should be made for any complications due to seasonality and management challenges associated with greater scale.

Next-generation sequencing survey of acute febrile illness in Senegal (2020-2022).

Acute febrile illnesses (AFI) in developing tropical and sub-tropical nations are challenging to diagnose due to the numerous causes and non-specific symptoms. The proliferation of rapid diagnostic testing and successful control campaigns against malaria have revealed that non-Plasmodium pathogens still contribute significantly to AFI burden. Thus, a more complete understanding of local trends and potential causes is important for selecting the correct treatment course, which in turn will reduce morbidity and mortality. Next-generation sequencing (NGS) in a laboratory setting can be used to identify known and novel pathogens in individuals with AFI. In this study, plasma was collected from 228 febrile patients tested negative for malaria at clinics across Senegal from 2020-2022. Total nucleic acids were extracted and converted to metagenomic NGS libraries. To identify viral pathogens, especially those present at low concentration, an aliquot of each library was processed with a viral enrichment panel and sequenced. Corresponding metagenomic libraries were also sequenced to identify non-viral pathogens. Sequencing reads for pathogens with a possible link to febrile illness were identified in 51/228 specimens, including (but not limited to): Borrelia crocidurae (N = 7), West Nile virus (N = 3), Rickettsia felis (N = 2), Bartonella quintana (N = 1), human herpesvirus 8 (N = 1), and Saffold virus (N = 1). Reads corresponding to Plasmodium falciparum were detected in 19 specimens, though their presence in the cohort was likely due to user error of rapid diagnostic testing or incorrect specimen segregation at the clinics. Mosquito-borne pathogens were typically detected just after the conclusion of the rainy season, while tick-borne pathogens were mostly detected before the rainy season. The three West Nile virus strains were phylogenetically characterized and shown to be related to both European and North American clades. Surveys such as this will increase the understanding of the potential causes of non-malarial AFI, which may help inform diagnostic and treatment options for clinicians who provide care to patients in Senegal.