cAMP Research Articles

High levels of mortality, violence, and distress: impact of the Syrian conflict on displaced people in Ein Issa camp, northeast Syria

High levels of mortality, violence, and distress: impact of the Syrian conflict on displaced people in Ein Issa camp, northeast Syria

Coverage of a pneumococcal conjugate vaccine campaign in Doro refugee camp, South Sudan and trends in inpatient lower respiratory tract infections over time

Coverage of a pneumococcal conjugate vaccine campaign in Doro refugee camp, South Sudan and trends in inpatient lower respiratory tract infections over time

Evaluating computer science camp topics in increasing girls' confidence in computer science

In the field of computer science, there is an evident lack of women. Studies show that girls begin to lose interest in the field from early on, so it is important for initiatives to get girls interested in coding from a young age. There are many outreach initiatives for young girls in computer science, with a wide array of topics. To help increase the number of women in computer science, we held a coding camp for middle and high school girls and offered three topics to choose from. The three camp topics included Scratch, Python, and Unity, and each were a week long. In this paper we look at the impact of the camp and compare the strengths of weaknesses of each topic in increasing girls' interest in coding and confidence in their abilities related to computer science.

A Child's Playground-A Syrian boy at the Moria Refugee Camp, Lesbos, Greece

The Moria Refugee camp in Lesbos, Greece is a surreal mix of an internment camp and shanty squatter encampment. Based in an old army compound it is defined by the steel …

WOW Summer Day Camp: Extension of an AAP Stage 3 Weight Management Program Emphasizing Cooking Skills, Enjoyable Activity, and Self-esteem.

Background: Pediatric overweight and obesity pose a serious health risk, affecting approximately 32% of US youth aged 2-19 years. Potential contributing factors include unhealthy weight gain in summer due to less structured activity and a decline in cooking skills potentially leading to poor food choices. To address these potential needs, as well as concerns around food insecurity, we initiated a pilot study that added a 5 day summer day camp to our AAP Stage 3 weight management program on the college campus that hosts our clinic. Methods: 13 children, enrolled …

Adventures in Communism: Counterculture and Camp in East Berlin

Abstract | This essay examines the encounter between Western countercultural groups and the urban landscape of East Berlin in the years immediately following the fall of the Berlin Wall in 1989. Focusing on squatted houses, the underground techno scene, and experimental art projects, the essay argues that countercultural groups who were active in East Berlin in the early 1990s developed a peculiar set of practices that were characterized both by their campy aesthetics and by their temporal indeterminacy. The essay posits that these experimental temporal practices were only possible due to the layered historicity of urban space in the dilapidated, inner-city neighbourhoods of East Berlin. Résumé | Cet essai étudie la rencontre entre les groupes contre-culturels Occidentaux et le paysage urbain de Berlin-Est suite à la chute du Mur de Berlin en 1989. A travers une analyse des squats, de la scène techno « underground » et des projets d’art expérimental, l’essai soutient que les groupes contre-culturels actifs dans le Berlin-Est du début des années 1990 ont développé un ensemble de pratiques caractérisé à la fois par leur esthétique maniérée et leur indétermination temporelle. L’essai avance que seule l’historicité imbriquée de l’espace urbain dans les quartiers pauvres et dilapidés du Berlin-Est a rendu possible ces pratiques temporelles expérimentales.

Effects of indomethacin on erythrocyte nitric oxide metabolism and erythrocyte deformability

Increased erythrocyte filtration index (decreased erythrocyte deformability; ED) was observed in patients with Behcet disease. When patients with Behcet took indomethacin, decreased values of erythrocyte acetylcholinesterase enzyme activity were obtained. An inverse association between nitric oxide (NO) efflux from erythrocytes and ED values was verified in blood samples of patients with vascular inflammatory diseases. The aim of this in vitro study was to verify the effect of indomethacin on ED, NO efflux, nitrite nitrate, peroxynitrite and nitroso- glutathione (GSNO) in the presence of protein tyrosine kinase (PTK Syk), protein tyrosine phosphatase (PTP), adenylyl cyclase (AC) and phosphatidylinositol 3 kinase (PI3K) enzyme inhibitors respectively Syk inhibitor, calpeptin, MDL and wortmannin (WORT). Blood samples from ten healthy donors were divided and aliquots were performed in absence (control aliquot) and presence of indomethacin or indomethacin plus each one of the inhibitors. Further ED and NO efflux and its metabolites were evaluated by the usual methodology. A significant increase in ED values was noted in the presence of indomethacin plus WORT. No significant differences where verified in NO efflux from erythrocytes in all blood aliquots. Significantly increase in GSNO concentration in the presence of indomethacin plus calpeptin, MDL or Wort were observed. Nitrate and peroxynitrite concentrations increased or decreased significantly respectively in all aliquots in relation to the control one. The nitrite levels increased in the aliquots with indomethacin and indomethacin plus MDL, or indomethacin plus Syk. In conclusion indomethacin act as antioxidant decreasing peroxynitrite, not changing NO efflux values and increasing nitrate levels independent of band 3 phosphorylation degree or cyclic adenosine monophosphate (cAMP) concentration. Indomethacin mobilizes nitrite and GSNO in dependant of band 3 phosphorylation degree and cAMP. Indomethacin did not change erythrocyte deformability, unless in combination with wortmannin which increased it.

Production of Cyclic Adenosine Monophosphate (cAMP) by Microbial Fermentation——A Review

Production of Cyclic Adenosine Monophosphate (cAMP) by Microbial Fermentation——A Review

Somatopause, weaknesses of the therapeutic approaches and the cautious optimism based on experimental ageing studies with soy isoflavones.

The pathological phenomenon of somatopause, noticeable in hypogonadal ageing subjects, is based on the growth hormone (GH) production and secretion decrease along with the fall in GH binding protein and insulin-like growth factor 1 (IGF-1) levels, causing different musculoskeletal, metabolic and mental issues. From the perspective of safety and efficacy, GH treatment is considered to be highly controversial, while some other therapeutic approaches (application of IGF-1, GH secretagogues, gonadal steroids, cholinesterase-inhibitors or various combinations) exhibit more or less pronounced weaknesses in this respect. Soy isoflavones, phytochemicals that have already demonstrated the health benefits in treated elderly, at least experimentally reveal their potential for the somatopausal symptoms remediation. Namely, genistein enhanced GHRH-stimulated cAMP accumulation and GH release in rat anterior pituitary cells; refreshed and stimulated the somatotropic system (hypothalamic nuclei and pituitary GH cells) function in a rat model of the mild andropause, and stimulated the GH output in ovariectomized ewes as well as the amplitude of GH pulses in the rams. Daidzein, on the other hand, increased body mass, trabecular bone mass and decreased bone turnover in the animal model of severe andropause, while both isoflavones demonstrated blood cholesterol-lowering effect in the same model. These data, which necessarily need to be preclinically and clinically filtered, hint some cautious optimism and call for further innovative designing of balanced soy isoflavone-based therapeutics.

Stable expression of the human TSH receptor in CHO cells and the functional assessment

Objective To establish and assess a CHO (Chinese Hamster Ovary) cell line that stably expressed the human TSH receptor. Methods The gene of human TSH receptor was amplified by PCR from the recombinant plasmid that had been constructed previously, and then the target gene was linked to linearized vector GV208. Afterwards, with the reconstructed vector and helper vectors, the target gene was packaged into the lentivirus. The recombinant lentivirus infected the CHO cells and integrated the gene into the chromatin. The established CHO cell line was isolated and cultured and its function was assessed. Results The result of gene test identified the correct sequence of DNA of the transformant. The expression of mRNA in CHO cells transfected with the human TSHR was obviously higher than the cells in control or blank group. Within the range of certain density, the more bovine TSH was used to stimulate the established CHO, the more cAMP would be produced. When stimulated with serum of patients of Graves′ disease, the cell line also significantly produced more cAMP. Conclusion The established CHO cell line transfected with the human TSHR can stably express the human TSH receptor and response to the stimulation of ligand of TSH receptor. (Chin J Endocrinol Metab, 2018, 34: 154-157) Key words: Human thyroid stimulating hormone receptor; Cell line; Establish; Function

Rethinking the lessons from Za'atari refugee camp

Rethinking the lessons from Za'atari refugee camp

Liraglutide Inhibits the Apoptosis of MC3T3-E1 Cells Induced by Serum Deprivation through cAMP/PKA/β-Catenin and PI3K/AKT/GSK3β Signaling Pathways.

In recent years, the interest towards the relationship between incretins and bone has been increasing. Previous studies have suggested that glucagon-like peptide-1 (GLP-1) and its receptor agonists exert beneficial anabolic influence on skeletal metabolism, such as promoting proliferation and differentiation of osteoblasts via entero-osseous-axis. However, little is known regarding the effects of GLP-1 on osteoblast apoptosis and the underlying mechanisms involved. Thus, in the present study, we investigated the effects of liraglutide, a glucagon-like peptide-1 receptor agonist, on apoptosis of murine MC3T3-E1 osteoblastic cells. We confirmed the presence of GLP-1 receptor (GLP-1R) in MC3T3-E1 cells. Our data demonstrated that liraglutide inhibited the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation, as detected by Annexin V/PI and Hoechst 33258 staining and ELISA assays. Moreover, liraglutide upregulated Bcl-2 expression and downregulated Bax expression and caspase-3 activity at intermediate concentration (100 nM) for maximum effect. Further study suggested that liraglutide stimulated the phosphorylation of AKT and enhanced cAMP level, along with decreased phosphorylation of GSK3β, increased β-catenin phosphorylation at Ser675 site and upregulated nuclear β-catenin content and transcriptional activity. Pretreatment of cells with the PI3K inhibitor LY294002, PKA inhibitor H89, and siRNAs GLP-1R, β-catenin abrogated the liraglutide-induced activation of cAMP, AKT, β-catenin, respectively. In conclusion, these findings illustrate that activation of GLP-1 receptor by liraglutide inhibits the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation through cAMP/PKA/β-catenin and PI3K/Akt/GSK3β signaling pathways.

Review: Imperial Bandits: Outlaws and Rebels in the China-Vietnam Borderlands by Bradley Camp Davis

Review: Imperial Bandits: Outlaws and Rebels in the China-Vietnam Borderlands by Bradley Camp Davis

CBP7 Interferes with the Multicellular Development of Dictyostelium Cells by Inhibiting Chemoattractant-Mediated Cell Aggregation.

Calcium ions are involved in the regulation of diverse cellular processes. Fourteen genes encoding calcium binding proteins have been identified in Dictyostelium. CBP7, one of the 14 CBPs, is composed of 169 amino acids and contains four EF-hand motifs. Here, we investigated the roles of CBP7 in the development and cell migration of Dictyostelium cells and found that high levels of CBP7 exerted a negative effect on cells aggregation during development, possibly by inhibiting chemoattractant-directed cell migration. While cells lacking CBP7 exhibited normal development and chemotaxis similar that of wild-type cells, CBP7 overexpressing cells completely lost their chemotactic abilities to move toward increasing cAMP concentrations. This resulted in inhibition of cellular aggregation, a process required for forming multicellular organisms during development. Low levels of cytosolic free calcium were observed in CBP7 overexpressing cells, which was likely the underlying cause of their lack of chemotaxis. Our results demonstrate that CBP7 plays an important role in cell spreading and cell-substrate adhesion. cbp7 null cells showed decreased cell size and cell-substrate adhesion. The present study contributes to further understanding the role of calcium signaling in regulation of cell migration and development.

Effectiveness of a Smoking Cessation Healing Camp for Male Workers in a Workplace Setting

Effectiveness of a Smoking Cessation Healing Camp for Male Workers in a Workplace Setting

The effect of science camp activity on scientifically gifteds' problem-solving ability and creative humanity

The effect of science camp activity on scientifically gifteds' problem-solving ability and creative humanity

«Рая весть в трёхмерности аду»: специфика художественного топоса лагерных стихотворений Анастасии Цветаевой

«Рая весть в трёхмерности аду»: специфика художественного топоса лагерных стихотворений Анастасии Цветаевой

A Not Too Greatly Changed Eden: The Story of the Philosopher's Camp in the Adirondacks by James Schlett

A Not Too Greatly Changed Eden: The Story of the Philosopher's Camp in the Adirondacks by James Schlett

Notes on the Uses of Black Camp

Notes on the Uses of Black Camp

A Not Too Greatly Changed Eden: The Story of the Philosophers’ Camp in the Adirondacks. By James Schlett. Ithaca: Cornell University Press, 2015. 264 pp. Illustrations, notes, select bibliography, and index. Cloth $29.95, paper $19.95.

<i>A Not Too Greatly Changed Eden: The Story of the Philosophers’ Camp in the Adirondacks</i>. By James Schlett. Ithaca: Cornell University Press, 2015. 264 pp. Illustrations, notes, select bibliography, and index. Cloth $29.95, paper $19.95.