A Significant essential oil is Eucalyptus oil (C10H18O). Fresh and dried leaves, as well as branch tips, are used to extract the oil. Bioactive properties, and a length history of usage against many disease make eucalyptus oil a valuable medicinal tool. The results showed that the essential oil considerably affected Trichophyton rubrum's growth in liquid medium (SDB), and that impact developed as concentration increased. In comparison to the control, which weighed 4.87 gm, the weight of the mycelium mat was 1.17, 2.83, 3.57 gm at concentrations of 100, 50, and 25%, respectively. The lowest weight was found at concentration 25%, which was 3.57 gm. The concentration of 100 percent yielded the most extreme level of inhibition (76.13%), while the concentration of 25% yielded the lowest level of inhibition (26.54%). The medium SDA showed the most noteworthy growth, with radial growth of 76.67 mm following 7 days, trailed by the medium PDA at 70.33 mm. The case of the medium YEA 20.33 mm showed the most minimal growth rate. A statistical analysis uncovered that there is no significant difference (P < 0.05) between the medium MEA and YEA, but there is one among SDA and every single tried medium. subsequently the accompanying tests can be performed utilizing the medium SDA.