Calyx Extract Research Articles

Pre-treatment with Hibiscus Sabdariffa Linn calyx Extract is Protective Against Sub-Chronic Paracetamol Hepatotoxicity in Mice

Many plant parts have been found to have efficient protective and ameliorative effects against drug induced liver injury. The present study aimed to investigate the prophylactic effects of Hibiscus sabdariffa Linn calyx aqueous extract (HSCE) on sub chronic paracetamol induced hepatotoxicity in mice. Twenty mice were randomly distributed into four groups of five mice each. The control and HSCE only group were given aqueous DMSO and HSCE (250 mg/kg b. wt.) respectively. Hepatotoxicity was induced in the mice by oral administration of paracetamol at a sub chronic dose (500 mg/kg b. wt.) in paracetamol only group. The pretreatment group were administered HSCE at zero time and paracetamol (500 mg/kg b. wt.) 8 h later. The blood and liver were collected and subjected to biochemical analysis after 8 weeks. The administration of paracetamol significantly (P ≤ 0.05) induced hepatotoxicity as evidenced by the increase in the level of liver marker enzymes and significant (P ≤ 0.05) decrease in the level of protein and albumin. Pretreatment with HSCE significantly (P ≤ 0.05) reduced serum levels of elevated liver enzyme markers. As regards to oxidative stress markers, pretreatment with HSCE significantly (P ≤ 0.05) decreased the level of malondialdehyde and increased the activities of superoxide dismutase and catalase. The hepatoprotective activity of HSCE was also confirmed by histopathological findings. These results suggest that pretreatment with HSCE has protective effect against paracetamol induced hepatotoxicity in mice.&nbsp

Effect of Different Extraction Methods on Anthocyanin Content in Hibiscus sabdariffa L. and their Antiplatelet and Vasorelaxant Properties.

Hibiscus sabdariffa L. is a worldwide component for tea and beverages, being a natural source of anthocyanins, which are associated with cardiovascular activities. To investigate this relationship, we explored different methods of aqueous extraction on the anthocyanin content and antioxidant activity of H. sabdariffa L. calyx extract (HSCE). Pharmacological effects via platelet aggregation, calcium mobilization, cyclic nucleotide levels, vasodilator-stimulated phosphoprotein Ser157 and Ser239, and on the vasomotor response of aortic rings isolated from mice are studied herewith. We found that the application of ultrasonic turbolization, 20min, combined with acidified water was significantly more effective in the extraction process, providing extracts with the highest levels of anthocyanins (8.73 and 9.63mg/100g) and higher antioxidant activity (6.66 and 6.78μM trolox/g of sample). HSCE significantly inhibited (100-1000μg/mL) arachidonic acid-induced platelet aggregation, reduced calcium mobilization, and increased cAMP and cGMP levels with VASPSer157 and VASPSer239 phosphorylation. Vasorelaxation reduction was confirmed by the aortic rings and endothelium assays treated with nitric oxide synthase inhibitors, soluble guanylyl cyclase (sGC) oxidizing agent, or Ca2+-activated K+ channel inhibitor. The increasing of cGMP levels could be understood considering the sGC stimulation by HSCE compounds in the specific stimulus domain, which allows an understanding of the observed antiplatelet and vasorelaxant properties of H. sabdariffa L. calyx extract.

Active compound analysis of ethanolic extract of roselle calyces (Hibiscus sabdariffa L.)

Roselle (Hibiscus sabdariffa L.) is a comestible plant known for its fleshy red calyces that are used in making a wide range of foods such as wine, juice, jam, syrup, pudding, cakes, ice cream, and herbal tea. The anti- bacterial, diuretic, antioxidant, and anti-mutagenic effects of the roselle calyces are also well known. It is high in vitamins, minerals and bioactive substances such as organic acids, phytosterols, and polyphenols, and because of its extensive phar-macological potential, it has long been used as folk medicine to treat common cold. Gas chromatography-mass spectroscopy (GC-MS) is the best technique to identify the compounds present in the sample by mass spectra data obtained from purely available standards injected under the same conditions. In this study, the GC-MS technique was used to validate the pharmacological potential of Hibiscus sabdariffa by identifying the chemicals found in its calyces. The maximum cyanidin-3-glucoside was found to be the highest in PKM (Periyakulam) HS 04 1784.65 mg/100 g in cyanidin-3-glucoside equivalents, total flavonoid content (28.01 mg QE/g), and DPPH (2-diphenyl-1-picrylhydrazyl) activity % (93.17), and in PKM HS 02 total phenolic content (1.29 GA mg/g). The extract was prepared by soaking a dry calyx powder sample in methanol overnight and the ethanolic extract was then analyzed using GC-MS. Flavonoids, tannins, phenols, saponins, alkaloids, glycosides, terpenoids, and steroids were found in the ethanolic extract of Hibiscus sabdariffa calyces. The existence of 26 bioactive chemicals was discovered by GC-MS analysis, including phthalic acid, astaxanthin, lutein, lycoxanthin, 3-Pyridinecarboxylic acid, rhodoxanthin, molybdenum, and hexadecenoic acid. The presence of some of these bioactive chemicals has been used to support scientific evidence for the plant’s anti-aging, anti-oxidant, anti-hypertensive, and anti-inflammatory capabilities, which constitute valuable preliminary information in pharma industries.

Assessment of Growth-Related Parameters, Immune-Biochemical Profile, and Expression of Selected Genes of Red Tilapia Fed with Roselle Calyces (Hibiscus sabdariffa) Extract

The purpose of this research was to determine whether or not supplementing a diet with ethanolic roselle calyces extract (ER) had any effect on the rate of growth, intestinal morphometry, total carotene in skin and muscle, blood profile, immunity status, and the expression response of red tilapia. The ER was added to four experimental diets at 0% (0 g kg−1), 0.5% (5 g kg−1), 1% (10 g kg−1), and 2% (20 g kg−1), which were designated as ER0 (control group), ER0.5, ER1, and ER2, respectively. The results show that ER1 induced higher weights (final weight, weight gain, specific growth rate, and weight gain rate) and all ER groups had considerably (p < 0.05) decreased feed conversion rates (FCR) compared with the control diet. Histomorphometric examination of the intestinal villi absorptive capacity showed fish given ER, specifically ER1, had increased villus length, width, and goblet cells (p < 0.05). The best hematological and biochemical parameters (the antioxidant enzyme activity of superoxide dismutase and catalase, lysozyme activity, and WBCs count) were observed for 5 g kg−1 ER. In addition, diets supplemented with different levels of ER stimulated phagocytic activity (p < 0.05). Additionally, the highest total carotene content in skin and muscle was observed in ER0.5. The 0.5, 1, and 2% roselle extract diets induced upregulation of IGF-1, GHr, SOD, TNF-α, and LPL, whereas MSTN, HSP 70, and FAS were downregulated. In conclusion, dietary ER supplementations are advantageous for red tilapia because they improve immunological and growth-related parameters.

Formulation Containing Phytosomes of Carotenoids from Nyctanthes arbor-tristis and Tagetes patula Protect D-galactose Induced Skin Aging in Mice

• Gel containing phytosomes of crocin rich extract(equivalent to crocin 1% w/w) obtained from Nyctanthes arbor-tristis and Lutein rich extract (equivalent to lutein 0.5%w/w) from Tagetes patula indicated • Entrapment of crocin and lutein was found to be (60.20% w/w) and (50.81% w/w) respectively. • Improved stability of crocin and lutein through preparation of Phytosomes • Significant (P<0.01) increase in thickness of dermal and epidermal layer • Significant increase (P<0.05) in glutathione level and decrease (P<0.01) in MDA level • Upregulation of expression of elastin and Collagen gene by 0.26 and 0.03 fold respectively : Carotenoids play important role in delay of aging process. Orange coloured tubular calyx of flowers of Nyctanthes arbor-tristis. contains crocin, an apocarotenoid which forms a major component of stigma of saffron. Due to presence of crocin in orange coloured tubular calyx of Nyctanthes arbor-tristis, it can be used as an economical substitute to saffron for its medicinal and cosmetic utilities. Lutein from flower petals of Tagetes patula L , is another popular carotenoid which has antioxidant effect and many health benefits. The carotenoids are highly unstable when exposed to atmosphere, hence in the present study crocin and lutein rich extracts were entrapped into phytosomes to improve stability and efficacy of topical preparation. : Oxidative stress is considered to be a major contributor to the process of aging. Carotenoids viz, Lutein and crocin, being powerful antioxidants, can delay aging of skin through upregulating col type I gene and elastin gene. The carotenoids have very low oxidation potential, due to which they get oxidized fast and protect oxidation of other compounds which make them highly unstable. One of the ways to improve stability of these phytoconstituents, is their entrapment in phytosomes. Preparation of phytosomes will have dual advantages of improving stability as well as bioavailability of molecule. : The phytosomes of Carotenoid rich extract of tubular calyx of Nyctanthes arbor-tristis L. and the petals of Tagetes patula L. (standardized for crocin and lutein content) were prepared using lipid film hydration technique and these phytosomes were then incorporated into gel base. The gel formulation was evaluated for stability as per ICH guidelines. Efficacy of formulation was evaluated by D-galactose induced aging model. Aging in skin was induced by administration of D-Galactose (100mg/kg b.w.s.c.) to albino mice for 42 days. The gel formulation was applied topically for 42 days. Then the effect of formulation on skin aging was evaluated by estimation of biochemical parameters viz. glutathione and malondialdehyde (MDA) and histopathological studies of treated skin samples. Also expression of COL type I and elastin genes was carried out from the skin samples by RT-PCR method. : Percent entrapment (%w/w) of crocin and lutein in phytosomes were found to be 60.20 and 50.81 respectively. Accelerated stability studies showed improvement in stability of carotenoids viz. crocin and lutein and the content of crocin and lutein in formulation was found in the range of 99.98 % w/w to 99.85%w/w at the end of three months. The formulation containing extract of Phytosomes of carotenoid rich extracts of Nyctanthes arbor-tristis L. and the petals of Tagetes patula L. exhibited potent antiaging activity through significant (p<0.05)increase in dermal and epidermal layers, and increase in GSH levels of skin as compared to the untreated group. The treatment with the gel formulation revealed upregulation of col type I and elastin genes. There was significant reduction in lipid peroxidation as revealed through reduction in MDA levels as compared to untreated group. : Crocin and lutein have potential to prevent skin aging via upregulation of COL type I gene and elastin gene. Upregulation of genes resulted into increase in the thickness of epidermal and dermal layer along with reduction in oxidative stress in skin. Entrapment of carotenoid rich extracts of Nyctanthes arbor-tristis and Tagetes patula in Phytosomes enhanced the stability and efficacy of the formulation.

Edible composite films based on chitosan/guar gum with ZnO[sbnd]NPs and roselle calyx extract for active food packaging

The present study aimed to prepare and characterize edible composite films. The edible composite films were made from a mixture of guar gum/chitosan with the loading of zinc oxide nanoparticles (ZnONPs) and roselle calyx extract (RCEx). The incorporation of RCEx and ZnONPs improved the antioxidant and mechanical properties of films. Based on yield of bioactive compounds obtained by the UAE method was used for the development of ZnONPs and the edible composite film. The Cross-section microstructure of the films showed that the films were dense and compact. Although the incorporation of RCEx decreased the transparency of the films, it enhanced the UV–Vis light barrier properties. The tensile strength, elongation at break, and solubility of CS/GG film, CS/GG/RCEx and, CS/GG/RCEx/ZnONPs film was found to be (35.74 MPa, 54.24 MPa and, 69.42 MPa), (15.56%, 12.27% and, 9.69%), and (67.23%, 59.41%, and 61.35%) respectively. Water vapor permeability (WVP) of the films was decreased from 4.68 to 4.06 (g /m2.day.kPa) after the addition of zinc oxide nanoparticles into the films, respectively. RCEx and ZnONPs incorporated films showed the higher antioxidant activity than control film, 11.52%, 42.55% and, 68.15% respectively. This study showed edible composite films incorporated ZnONPs and RCEx have promising potential for applications in active food packaging in the future.

Nephroprotective effect of Physalis peruviana L. calyx extract and its butanolic fraction against cadmium chloride toxicity in rats and molecular docking of isolated compounds

BackgroundCadmium is an environmentally toxic metal that has deleterious effects on both animals and humans due to its accumulation in different body tissues. Physalis peruviana L. fruit and calyx contain many active constituents which are used traditionally for their different biological activities. Based on the traditional uses of P. peruviana L. calyx, we aimed to evaluate the nephroprotective effect of their 80% aqueous methanol extract (AME) and n-butanol fraction (Bu.F.) against cadmium chloride-induced nephrotoxicity in rats and to correlate this activity with phytoconstituents isolated using molecular docking studies.MethodsThe n-butanol fraction of P. peruviana L. calyx was fractionated using various chromatographic techniques and the isolated compounds were identified based on their chemical and spectroscopic data. The nephroprotective activity was assessed using cadmium chloride-induced nephrotoxicity in the rat model, by measuring some important parameters such as body weight, kidney weight, serum urea, and creatinine levels, oxidative stress markers, inflammatory markers, and histopathological examinations of kidney tissue. Molecular docking studies of the isolated compounds were performed.ResultsThree withanolides named 4 β-hydroxywithanolide E (1), Physalin B (2) and 3α, 14β-dihydroxywithaphysalin N (3) were isolated and identified from the n-butanol fraction of P. peruviana L calyx extract. The extract and its butanol fraction significantly improved the serum kidney function markers and tissue oxidative status including malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT). Additionally, the extracts significantly decreased the levels of tumor necrosis factor-alpha (TNF-α) and nuclear factor kappaB (NF-κβ). Moreover, the histological changes were ameliorated by the extracts. The molecular docking study showed that the isolated compounds displayed a remarkable inhibitory activity against IκB kinase.ConclusionThe AME and its butanol fraction of P. peruviana L calyx showed potential nephroprotective activity against cadmium chloride-induced nephrotoxicity which is correlated at least in part to its considerable withanolides content.

Quality assessment of yoghurt formulated with aqueous extract of Roselle calyx, Moringa leaf and Lemon grass

Quality assessment of yoghurt formulated with aqueous extract of Roselle calyx, Moringa leaf and Lemon grass

Beneficial Effects of Hibiscus sabdariffa in Diabetic Wistar Rats

Hibiscus sabdariffa is a yearly shrub that is widely grown in some Asian and African countries including Nigeria where it is commonly prepared as a hot or cold beverage and also used as medicine. It is applied in folklore remedies in the treatment of some ailments. This study was aimed at investigating the effects of the aqueous calyx extract of Hibiscus sabdariffa on some biochemical parameters of diabetic male Wistar rats. The rats were divided into four (4) groups of five (5) rats each. Group one (1) which served as non diabetic (negative) control received distilled water only. Group 2 served as diabetic control and received distilled water after alloxan-induced diabetes. Group three (3) and group four (4) were diabetic rats that received 250mg/kg bw and 500mg/kg bw of the aqueous calyx extract of Hibiscus sabdariffa respectively, for a period of four (4) weeks. The Statistical Program for Social Sciences (SPSS version 21.0) was used for the analysis. Differences between group means was tested using analysis of variance (ANOVA) and compared using the post hoc test. P&lt;0.05 was considered statistically significant. The results obtained showed that the higher dose of the extract caused a significant decrease in blood glucose level in 4 weeks of the study. The changes observed in the plasma concentrations of electrolytes indicated that the extract significantly improved diabetes induced decrease in Cl- level while changes in Na+, K+ and HCO3- were not statistically significant. It also significantly improved lipid profile and reduced oxidative stress in diabetic male Wistar rats. This study revealed that, the calyx extract of Hibiscus sabdariffa demonstrated anti-hyperglycemic effects in diabetic Wistar rats.

Extract of Calyces from Physalis peruviana Reduces Insulin Resistance and Oxidative Stress in Streptozotocin-Induced Diabetic Mice.

Diabetes mellitus is a metabolic disorder mainly characterized by obesity, hyperglycemia, altered lipid profile, oxidative stress, and vascular compromise. Physalis peruviana is a plant used in traditional Colombian medicine for its known activities of glucose regulation. This study aimed to evaluate the anti-diabetic activity of the butanol fraction from an extract of Physalis peruviana calyces in two doses (50 mg/kg and 100 mg/kg) in induced type 2 diabetic mice. Blood glucose levels were evaluated once a week, demonstrating that a dose of 100 mg/kg resulted in greater regulation of blood glucose levels in mice throughout the experiment. The same overall result was found for the oral glucose tolerance test (OGTT) and the homeostatic model assessment for insulin resistance (HOMA- IR). The lipid profile exhibited improvement compared to the non-treated group, a dose of 100 mg/kg having greater protection against oxidative stress (catalase, superoxide dismutase, and malondialdehyde levels). Histopathological findings in several tissues showed structure preservation in most of the animals treated. The butanol fraction from Physalis peruviana at 100 mg/kg showed beneficial results in improving hyperglycemia, lipidemia, and oxidative stress status, and can therefore be considered a beneficial coadjuvant in the therapy of diabetes mellitus.

Nutritionally Enriched Muffins from Roselle Calyx Extract Using Response Surface Methodology.

Hibiscus sabdariffa, often called Roselle, is a flowering plant with a variety of traditional medicinal uses. Its calyx, with a bright and attractive red color, produces a tart and pleasant acidic taste. The purpose of this study was to develop a Roselle muffin and assess the acceptability, nutrition, and shelf life of the muffin using its ingredients. The muffin was developed using different formulations in different proportions resulting from Response Surface Methodology (RSM). Sensory parameters were used to assess the muffin's acceptability. According to the findings, the combination of extract volume 45.37 mL, citric acid 1.11 g, and sodium bicarbonate 1.67 g produces the best muffin, with the panelist's sensory scores reaching up to 84%. The outcome of the study suggests muffins baked with the Roselle calyx extract have high antioxidant (12.53 ± 0.13)%, anthocyanin (126.63 ± 1.96) mg Cyn-3-glu/100 g, phenolic (12.91 ± 0.69) mg GAE/100 g, and ascorbic acid (12.10 ± 0.89) mg/100 g contents. The microbial shelf life of the developed muffin is estimated to be 6 days at room temperature. The study findings can therefore be utilized in the development of foods containing Roselle calyx extract.

Development of a single stage roselle calyx extractor

An 8-litre capacity roselle calyx extractor was developed with Arduino based temperature controls ranging from 30 to 100⁰C. Performance evaluation conducted on the extractor showed that efficiency of the extractor increased as the weight of the calyces decreased at constant mass of water which ranged from 55% to 89%. The effect of extraction time (5, 10 and 15 minutes), process temperature (30, 50, 75 and 100⁰C) and calyx-water mass ratio (1:50, 1:20 and 1:10) on the pH, density and colour of the extract was determined. The pH ranged from 2.4 to 2.9 and density was from 1.02 to 1.05gcm-3. Statistical analysis conducted showed that temperature and calyx-water mass ratio has a linear significant effect (p&lt;0.001) on all the responses while time had a linear significant effect (p&lt;0.001) on all responses except density. Time and temperature had interaction significant effect (p&lt;0.01) on pH, while temperature and calyx-water ratio had interaction significant effect (p&lt;0.01) on the density of the extract. Calyx-water ratio had a quadratic significant effect (p&lt;0.001) on the pH and colour of the extract. The contour plots presented the interactions between the factors and their responses. This study demonstrated that roselle calyces extract can be produced more efficiently than practices currently available and also that processing conditions are very important in the quality of extracts produced.

Porous Biodegradable Sodium Alginate Composite Fortified with Hibiscus Sabdariffa L. Calyx Extract for the Multifarious Biological Applications and Extension of Climacteric Fruit Shelf-Life

Porous Biodegradable Sodium Alginate Composite Fortified with Hibiscus Sabdariffa L. Calyx Extract for the Multifarious Biological Applications and Extension of Climacteric Fruit Shelf-Life

Antidyslipidemic, Antioxidant, and Anti-inflammatory Effects of Jelly Drink Containing Polyphenol-Rich Roselle Calyces Extract and Passion Fruit Juice with Pulp in Adults with Dyslipidemia: A Randomized, Double-Blind, Placebo-Controlled Trial.

Oxidative stress and inflammation play key roles in the pathophysiology in the pathophysiology of dyslipidemia, which are positive risks that increase atherosclerosis leading to important healthcare problems. Therefore, we aimed to study the antioxidant, anti-inflammatory, and lipid-lowering effects of jelly drink containing polyphenol-rich roselle calyces extract and passion fruit juice with pulp concentrate (RP jelly drink) in comparison to a placebo jelly drink for 8 weeks. Forty-three adults with dyslipidemia were randomly assigned into two groups: the RP jelly drink group and the placebo group. Glucose, total cholesterol (TC) triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), oxidative stress biomarkers, inflammatory parameters, and monocyte chemotactic protein-1 (MCP-1) were measured with fasting blood samples at baseline, 4 weeks and 8 weeks of intervention. Results showed a significant decrease in LDL-C and TG, respectively, after 8 weeks of RP jelly drink consumption (LDL-C: 107.63 ± 22.98 mg/dL; TG: 109.79 ± 38.83 mg/dL) compared to baseline measurements (LDL-C: 128.43 ± 32.74 mg/dL; TG: 132.33 ± 75.11 mg/dL). These may be possible due to reduced inflammation and improvements in oxidative stress, as demonstrated by the reduction of tumor necrosis factor- (TNF-) α and malondialdehyde (MDA), and the enhancement of glutathione (GSH) after consuming the RP jelly drink for 8 weeks. However, no significant differences of treatment on glucose, total cholesterol, MCP-1, interleukin-6, and interleukin-10 were observed. In conclusion, daily consumption of RP jelly drink for 8 weeks resulted in significant improvement in lipid profiles in subjects with dyslipidemia. However, more research is needed to assess its nutritional and functional potential.

Influence of different irrigation regimes and planting times on the quality and quantity of calyx, seed oil content and water use efficiency of roselle (Hibiscus sabdariffa L.)

This study was carried out to examine the physiological traits, quality of calyx extract and seed oil content of roselle (Hibiscus sabdariffa L.) as affected by irrigation regimes and planting dates. The growth period from seed sowing to calyx harvesting was shortened as planting time was delayed. Stem diameter and plant height were decreased by drought stress or late planting, but calyx yield, total phenolic content, total anthocyanin content, vitamin C, and calyx water use efficiency increased under mild drought condition. In addition, antioxidant activity and calyx water use efficiency were significantly increased by late planting. It was suggested that an increase in calyx harvest index in delay in planting would be due to better photosynthesis activity and higher assimilate use efficiency because of the increase in sink capacity. Seed oil content decreased considerably due to drought stress and delay in planting date. These findings suggest that mild drought stress improves the quality and quantity of calyx and water use efficiency.

Metabolomics combined to chemometrics reveals the putative α-glucosidase and α-amylase inhibitory metabolites of ground cherry (Physalis pruinosa L.)

In this work, metabolic profiling of the different parts of ground cherry (P. pruinosa) including fruits, calyces, leaves, stems and roots using UPLC-MS/MS analysis combined to chemometric analysis was attempted. A total of 82 chromatographic peaks belonging to different metabolite classes were identified including terpenes, flavonoids genin and glycosides, withanolides, physalins, sucrose esters, fatty acids and other miscellaneous compounds with withanolides being the most predominant class. Roots extracts possessed the highest relative content of the identified 5β,6β-epoxy withanolides and intermediate withanolides, while sucrose esters and flavonoidal glycosides were found in a great abundance in calyces extracts. Moreover, physalins were found in all extracts except for roots extracts. Studying the coefficients plots revealed that terpenes and physalins (physanicantriol, loliolide, physalisitin C) were responsible for discrimination of fruits extracts. Calyces, leaves and stems extracts were found to possess antioxidant activity and higher inhibition of α-glucosidase activity. In an attempt to identify the compounds responsible for the hypoglycemic activity using both α-amylase and α-glucosidase inhibition assays, OPLS models coefficient plots were constructed which indicated that physangulide B, physaperuvin G, neophysalin A, and acylsucroses were positively correlated to α-glucosidase inhibition, while guaiacyl-primeveroside, phyperunolide C, physalactone, physalolactone C and perulactone, were positively correlated to α-amylase inhibitory activity.

Computer-Aided Identification of Cholinergic and Monoaminergic Inhibitory Flavonoids from Hibiscus sabdariffa L.

The reduced levels of acetylcholine and dopamine lead to Alzheimer's disease (AD) and Parkinson's disease PD, respectively, due to the action of cholinesterase and monoamine oxidase B. Therapeutic options for AD and PD involve respective cholinergic and monoaminergic inhibitors, and considering the adverse outcomes of cholinergic- and monoaminergic- inhibitory therapeutics, phytoconstituents may be promising alternatives. Reports have shown that different extracts of the calyx of Hibiscus sabdariffa exhibit anticholinesterase and monoamine oxidase B inhibitory properties with the potential to delay and prevent the development of AD and PD. However, there is limited knowledge on the multitarget cholinergic and monoaminergic inhibitory activities of individual compounds in this plant. Computational methods were used to identify the specific compounds responsible for the observed cholinergic and monoaminergic inhibitory activities of the H. sabdariffa calyx extracts. Results confirm that three flavonoids: delphinidin-3-sambubioside, kaempferol-3-O-rutinoside and quercetin-3-rutinoside showed strong binding affinity with acetylcholinesterase, butyrylcholinesterase and monoamine oxidase B while the observed stability of the ligands-enzymes complexes over the MD simulation time suggests their cholinergic and monoaminergic inhibitory properties. The three flavonoids may be responsible for the reported anticholinergic and monoaminergic inhibitory potentials of H. sabdariffa extracts and could be enlisted as multi-target inhibitory agents for cholinesterases and monoamine oxidase B.

The uterotonic screening of calyx extracts of Hibiscus sabdariffa on estrogenized isolated Uterus of Wister strain albino rats

The claim of the use of orally consumed concentrated Hibiscus sabdariffa calyx preparation for the purpose of abortion in view of its widely uses as a beverage in the present period of economic hardship where it is a preferred beverage due to its affordability, naturalness and for its numerous medicinal properties has posed another element of concern to the female folks. This study was aimed to screen extracts of H. sabdariffa calyx of its acclaimed uterotonic activity. Contractile responses of estrogenized isolated rat uterus using various volumes (0.1, 0.2, 0.4, 0.6, 0.8, 1.0,1.6 ml) were established with oxytocin (1x10-4 g/ml), ergometrine (1x10-4 g/ml), misoprostol (1x10-4 g/ml), Acetylcholine (1x10-3 g/ml) and five different extracts of H. sabdariffa - ethanol (2.0x10-3 g/ml), methanol (2.0x10-3 g/ml), acetone (2.0x10-3 g/ml), cold water (2.0x10-3 g/ml) and hot water (2.0x10-3 g/ml). After these, nifedipine, acetylcholine and salbutamol were used to challenge the maximal responses of the isolated uterine tissue to oxytocin, acetylcholine and the cold aqueous extract of H. sabdariffa (CWEHs). The CWEHs was equally potent at inducing contraction activity compared to the standard drugs. This study further shows reduction in the amplitude of contraction produced by CWEHs in the presence of Promethazine (1×10−6 g/ml) and Nifedipine (1×10−6 g/ml). The blockade is more pronounced in promethazine compared to Nifedipine. Aqueous extract of Hibiscus sabdariffa calyx possess uterotonic activity on estrogenized isolated rat uterine muscle with mechanism of action related to histamine receptors and contraction of the myometrial cells as the was antagonized by promethazine and Nifedipine. This could be a justification for its alarming use as an abortifacient.

Roselle Calyx Extract as an Antioxidant Supplement on Tris-Base Extender for Goat Semen

In this study, we used extract from Roselle (Hisbiscus sabdariffa L.) Calyx to determine its ability as an antioxidant supplement to improve motility, progressive score and abnormality of goat’s sperms. Pooled semen from four male goats with average of live body weight 34.4 ± 0.6 kg and body condition score 2.5 ± 0.1 were aliquot into two groups: Control group; 0 mg/ml and Treatment groups; (Treatment 1:13 mg/ml, Treatment 2: 20 mg/ml, Treatment 3: 33 mg/ml, Treatment 4: 43 mg/ml of Roselle’s extract. All the semen parameter was observed at 0 hour, 0.5 hour, 1.0 hour, 1.5 hours after semen collection. The supplementation of extender with 13 mg/ml, 20 mg/ml, 33 mg/ml, 43 mg/ml of extract from Roselle Calyx does not affect sperm motility, progressive score and morphology of goat (p&gt;0.05). However, all sperm, regardless with supplementation and without supplementation of Roselle extract were maintained in good quality (60%) up to 1.5 hours. It is quite difficult to conclude that there are no effect of Roselle extract on sperm quality due to arise possibility of factors that may contribute to inefficiency effect of Roselle extract on sperm parameter. There were high possibility that the causes are due to inefficiencies of extraction method and/or degradation of anthocyanin pigment during extraction and storage process. Unsuitable extraction method could results in low yield and unstable of anthocyanin pigment. Moreover, temperature, pH and light causes the pigment to become unstable during processing and storage leading to degradation. Thus we suggest anthocyanin content monitoring in extract Roselle Calyx are required for future study.

The Effect of Acute Administration of the Aqueous Calyx Extract of Hibiscus sabdariffa on Blood pressure, Heart rate and Double Product of Apparently Healthy Human Subjects during Different Postures.

The aqueous calyx extract of Hibiscus sabdariffa (HS) is widely consumed as a beverage in Northern Nigeria and other parts of the world. HS has been reported to lower blood pressure (BP) in animals and man. However, not much is known about the effect of HS on BP in different postures. We tested the hypothesis that HS may lower BP, heart rate (HR) and heart rate-pressure product or double product (DP) by attenuating the discharge of the autonomic nervous system in different postures. Experiments were performed in accordance with the Principles of the Declaration of Helsinki. Following ethical approval and informed consent, BP and HR were measured in different postures (supine, sitting and standing) in apparently healthy human subjects (n=20) before and after (+HS) the oral administration of 15mg/Kg HS. Mean arterial pressure (MAP; taken as representative BP) and DP were calculated. Results are expressed as mean ±SEM. Paired t test and ANOVA with a post hoc Bonferroni test were used for statistical analyses. P<0.05 was considered significant. In the supine position MAP, HR and DP were significantly (P<0.0001 each) reduced in the presence of HS (85.6±1.7mmHg, 72.1±1.1/min and 8716±320mmHg.bpm) compared to its absence (89.6±2.0mmHg, 73.7±1.6/min and 8921±444mmHg.bpm). Similar trends were observed in the sitting position in the presence of HS (85.4±2.7mmHg, 73.7±1.8/min and 9098±345mmHg.bpm vs its absence: 91.4±2.3mmHg, 77.1±1.9/min and 9388±478mmHg.bpm; P<0.0001, P<0.0001 and P=0.007 respectively) and in the standing position (+HS: 89.3±2.0mmHg, 78.1±1.8/min and 10164±230mmHg.bpm vs its absence: 94.3±2.1mmHg, 81.8±2.3/min and 10742±268mmHg.bpm; P<0.0001, P<0.0001 and P=0.007 respectively). In the absence of HS, HR and DP were significantly higher in the standing posture (81.8±2.3/min, 10742±268mmHg.bpm) compared to the sitting (77.1±1.9/min, 9388±478mmHg.bpm; P<0.05 and P<0.0001 respectively) and the supine (73.7±1.6/min, 8921±444mmHg.bpm; P<0.001 each) postures while the BP remained similar. A similar trend was observed across the three postures in the presence of HS although the parameters were significantly lower. It is concluded that HS lowered BP, HR and DP by modulating autonomic mechanisms through the inhibition of both parasympathetic withdrawal and sympathetic nervous system discharge across the postures. Also the standing posture is associated more with a higher sympathetic nervous system discharge and a higher cardiac oxygen demand and workload than the sitting and supine postures in the absence or presence of HS.