Mesophyll protoplasts were isolated from young leaves of Nemesia strumosa (Scroph.), using pectinase and cellulase “Onozuka SS”. An optimum of cell wall regeneration was found at p H 5.6. Cells derived from protoplasts showed maximal divisions in the medium of N itsch and O hyama (1971): 14 days after protoplast isolation 79 % of the protoplasts had survived on Nitsch medium, and 92% of these surviving cells had divided. The medium of N agata and T akebe (1971) proved to be best suited for the subsequent protoplast callus subculture. Shoots and roots could be induced on the same medium containing various combinations of NAA and 6-BA. At the beginning of the induction period shoot formation could be observed only on media containing 6-BA, later on even on media containing NAA alone. The regenerative capacity of Nemesia protoplast callus diminishes rapidly with the increasing duration of culture.