Stingless bee honey is one of the most valuable insect products. The increasing popularity of stingless bee honey can be attributed to its composition, which has been linked to medicinal properties. Beekeeping with stingless bees is well-known in Indonesia, with Heterotrigona itama is the most popular stingless bee species cultivated in East Kalimantan, Indonesia. Stingless bees utilize various plant species as sustenance sources. Among those plants, Calliandra calothyrsus is popular planting in Indonesian bee plantations. This study analyzed the antioxidant (DPPH assay), phytochemical (qualitative method), water, and sugar content of H. itama stingless bee honey collected from a C. calothyrsus plantation. The results show that the water and sugar contents of the honey in this study were higher than in other research. Meanwhile, antioxidant capacity was also higher than in other studies. The phytochemical contents detected from honey in this study were carotenoids, coumarins, flavonoids, saponins, steroids, tannins, and triterpenoids. Even though the properties of stingless bee honey can differ based on vegetation and geographical origin, H. itama stingless bee honey collected from C. calothyrsus plantation in East Kalimantan, Indonesia, showed potential antioxidant activity and phytochemical content, which is advantageous to human health.
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