Phytophthora capsici is a major soil-borne oomycete plant pathogen, which severely affects the productivity of peppers including Capsicum annuum L., by causing fruit rot, root rot, and foliar blight diseases. It has a broad host range beyond Capsicum species and evolves rapidly to find new potential hosts for survival under different climatic conditions. In the present study, P. capsici was isolated from soil using castor seed baiting, and characterized through growth patterns and spore morphology. The species identification and molecular characterization were achieved through multi-locus sequence and phylogenetic analysis of three genomic loci (ITS, tef1 and cox), and also attributed given P. capsici isolate Pc_UHF into India-specific ITS-based evolutionary lineage Pc-X. Interestingly, dN/dS estimates revealed reasonably high positive selection in the tef1 gene, which could have possible implications on the pathogen's adaptation and/or virulence. Pathogenicity of this isolate was tested in three commercially growing bell pepper varieties and also in other crop species using detached leaf and/or in planta disease screening. Differential disease symptoms were induced by Pc_UHF in three bell pepper varieties, such as both collar rot and foliar blight symptoms observed in "Yolo Wonder" and "California Wonder", whereas only foliar blight symptoms were recorded in "Solan Bharpur". Furthermore, cross-pathogenicity was also recorded in tomato, citrus, cucumber and eggplant. This is the first report of molecular characterization and cross-pathogenically assessment of P. capsici isolate from farm soils in the North Western Himalayan region of India, which has a dynamic virulence profile and distinct host range.