Identification of the race of root knot nematode by differential host test method

Abstract

Root knot nematode (Meloidogyne incognita) cause major damage to the fruit crops, vegetable crops and field crops. Infected plants showed declined symptoms and poor fruit yield also displayed stunting and yellowing symptoms. In order to choose appropriate management control techniques, nematode diagnosis and specimen identification must be accurate, quick and precise. The population of the nematode obtained from the experimental field of Post Graduate Institute, Mahatma Phule Krishi Vidyapeeth, Rahuri were assessed for their host-race status by the differential host test which relies on the combination of resistant and susceptible reactions of six differential hosts to the nematode population. Result revealed that the nematode population infected tobacco (NC 95), pepper (California Wonder), watermelon (Charleston grey), tomato (Rutgers) and unable to reproduce on cotton (Deltapine- 16) and peanut (Florunner) which indicated presence of race 2 of Meloidogyne incognita.