Alternaria late blight of pistachio caused by Alternaria alternata, has become a serious problem in pistachio orchards in California. As part of the California pistachio improvement program, we evaluated the resistance/susceptibility of the breeding progenies to Alternaria late blight at two locations. The heritability of resistance ranged from 0.35 to 0.38 based on half-sib progenies analysis. Open-pollinated (OP) progenies from three cultivars showed moderate to high resistance. Greenhouse inoculation confirmed that OP progenies of cultivars Bronte and Trabonella had the greatest resistance. OP progenies of cultivar Red Aleppo were highly susceptible in greenhouse inoculations compared to moderate resistance found in field evaluations. OP progenies of the only commercial cultivar Kerman in California were susceptible in both field and greenhouse evaluation. The results show the potential for development of resistant cultivars is available in the breeding population of the California pistachio improvement program.