California Mastitis Test Research Articles

ISOLATION, IDENTIFICATION AND ANTIMICROBIAL PROFILE OF CORYNEBACTERIUM BOVIS FROM SELECTED DAIRY FARM IN BISHOFTU, CENTRAL ETHIOPIA

From January to May 2018, a cross-sectional study was undertaken on lactating dairy cows in Bishoftu town to isolate Corynebacterium bovis, determine the prevalence and risk factors, and evaluate the effectiveness of several antibiotics in lactating dairy farms. Study animals were selected randomly from selected dairy farms in the area. Collecting milk samples from mastitic cows, cultivating, and then performing an antibiotic sensitivity test were the procedures followed. A total of 384 lactating dairy cows were examined with inspection and California Mastitis Test (CMT), in which 86 of them were found to be CMT positive. Accordingly, prevalence was 3.9% and 18.5% for cows affected by clinical and subclinical mastitis respectively. The prevalence of mastitis showed statistically significant difference between, lactation stage, breed, age and washing (p > 0.05). However, there was no statistically significant difference noted in animal husbandry practice (p > 0.05). A total of 384 lactating dairy cows were examined with inspection and CMT, in which 86 of them were found to be CMT positive. Out of the 86 mastitis positive samples (sample indicates milk from one cow) sent to microbiology laboratory for microbiological examination, 7 bacterial isolates were identified as Corynebacterium bovis. The biochemical and morphological characteristics of 7 (1.8%) C. bovis isolated from bovine milk samples and the C. bovis reference strains were found to be uniform. Valuable criteria for identification were presence of catalase and oxidase, production of acid from glucose and a requirement for enriched basal media. C. bovis isolates have revealed a higher sensitivity to the kanamycin and streptomycin (71.4% each). A certain resistance has been noted to oxytetracycline (71.4%) and nalidixic acid (42.8%). Higher number of isolates showed moderate sensitivity or resistance to amoxicillin (51.1%). Regarding to multidrug resistance, the study reflects that only one isolate (14.3%) shows multidrug resistance to four drugs namely kanamycin, amoxicillin, nalidixic acid and oxytetracycline. This study demonstrated that mastitis due to C. bovis is rare in lactating dairy farms in Bishoftu. Some of the risk factors for mastitis can be addressed by practical management of dairy cows. Farm owners should selectively use the antibiotics to which the bacteria do not show resistance, such as streptomycin and kanamycin.

Prevalence, antimicrobial susceptibilities and risk factors of Methicillin resistant Staphylococcus aureus (MRSA) in dairy bovines

Staphylococcus aureus is a common mastitis pathogen in dairy cows, and methicillin-resistant S. aureus (MRSA) has been found in dairy farms all over the world. The study carried out on bovines from three governorates in Egypt, with the goal of determining the prevalence of MRSA in positive milk samples of subclinical mastitis, performing an antibiotic susceptibility test against MRSA isolates and determining the risk factors associated with MRSA. A total of 350 quarter milk samples (n = 200 mixed breed cow; n = 150 water buffalo) were collected and examined for subclinical mastitis using the California mastitis test (CMT) before being exposed to standard microbiological procedures for S. aureus isolation. The disc diffusion method was used to phenotypically analyse the positive S. aureus isolates for MRSA, which was verified by a PCR assay targeting the mecA gene. According to the findings of the study, 41.4% (145/350) milk samples were positive based on CMT, while 35.7% (125/350) of positive samples identified as MRSA based on PCR assay. However, the obtained results revealed non-significant disparity between cattle and buffalo and all predicted risk factors were strongly associated with prevalence of subclinical mastitis. The in-vitro antibiotic susceptibility test revealed that cefoxitin was completely resistant, whereas linezolid, ciprofloxacin, and trimethoprim + sulphamethoxazole were sensitive against the MRSA isolates. The relevance of S. aureus to public health, as well as the development of resistance to antibiotics like methicillin, needs ongoing testing of antimicrobial medications against MRSA isolates.

Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon.

Coliform bacteria are major causative agents of bovine mastitis, a disease that has devastating effect on dairy animal health and milk production. This cross-sectional study, carried out in the North West region of Cameroon, sought to determine the prevalence of bovine mastitis, coliforms associated with bovine mastitis, risk factors for infection and the antibiotic resistance pattern of coliform bacterial isolates. A total of 1608 udder quarters were sampled from 411 cows using a questionnaire, clinical examination, California Mastitis Test and milk culture. Primary isolation of coliform bacteria was done on MacConkey agar while identification of coliforms employed Gram-staining and biochemical testing. Each coliform bacterial isolate was challenged with 11 antibiotics using the Kirby-Bauer disc diffusion method. The prevalence of mastitis was 53.0% (218/411) and 33.1% (532/1608) at the cow- and quarter-levels respectively. Overall, 21.9% (90/411) cows and 8.2% (132/1608) udder quarters showed coliform mastitis. Escherichia coli was isolated in 7.0% of mastitis milk, and other coliforms isolated were Enterobacter cloacae (12.6%), Klebsiella pneumoniae (2.4%), Enterobacter sakazakii (1.1%), Klebsiella oxytoca (0.8%), Citrobacter freudii (0.4%), Serratia ficaria (0.4%) and Serratia liquefaciens (0.2%). Lactation stage, breed, history of mastitis and moist/muddy faeces contaminated environment were significantly associated (P-value < 0.05) with coliform mastitis. Coliform isolates (99.0%; 203/205) were resistant to at least one antibiotic tested. Amoxicillin had the highest resistance (88.8%) while norfloxacin had the least resistance (3.4%). Multidrug resistance was exhibited by 52.7% (108/205) of the isolates in a proportion of 27.8% Enterobacter cloacae, 10.7% E. coli, 6.3% Klebsiella pneumoniae, 2.9% Enterobacter sakazakii, 2.0% Klebsiella oxytoca, 1.0% Citrobacter freundii, 1.0% Serratia ficaria, 0.5% Serratia liquefaciens and 0.5% Serratia odorifera. Results indicate a need to educate these dairy farmers about mastitis (particularly subclinical), proper hygiene methods in milking and the public health implications of consuming contaminated raw milk.

Influence of mastitis on reproductive parameters in holstein-freisian cows

As mastitis is one of the most significant causes of health problems in dairy cows and, besides economic, also has other negative effects, the aim of this paper is to study the influence of mastitis on reproductive parameters in cows that have an evident and clinically obvious mastitis. In this study we observed the duration of the “service period“ and the number of inseminations (frequency of abortions) as reproductive parameters, up to the time of the next conception of lactating cows. Our research was carried out on a farm with Holstein-Friesian cows in duration of 15 months with breaks. First, 30 heads of cattle of various age were selected, the number of their lactations from 3 to 6. We took samples from the whole amount of milk twice a month in order to find out the number of somatic cells on the farm. The California Mastitis Test was carried out in all selected cattle. From all of the quarters of selected cows which showed a positive reaction with CMT, we took samples of udder (milk) secretion and sent them to the laboratory in the “Dr. Vaso Butozan” Veterinary Institute to estimate the number of somatic cells. Highly sophisticated equipment Fossomatic FC was used to identify the found microflora for these purposes. In all of the 30 cows a secretion disorder was found in one quarter: in 3 cows – in the front left quarter; in 5 cows - in the front right one; in 12 cows - in the back left one and in 10 cows - in the back right udder quarter. After the appropriate therapy was administered, we took the samples of udder secretion again in order to estimate the number of somatic cells. By this study it was estimated that the calving to conception interval in cows with clinical mastitis was ±76 days and the insemination index 2.6. The calving to conception interval referring to the farm in whole was ±53.2 days and the insemination index 1.96. Clinical mastitis followed by the increase in the number of SC prolongs the calving to conception interval to the significant extent, i.e., increases the insemination index.

Antibiogram, Haemolysis and Biofilm Properties of Escherichia coli from Bovine Mastitis

Background: Escherichia coli cause mammary gland inflammation in dairy cows with striking local and sometimes severe systemic clinical symptoms. This disease affects many high producing cows in dairy herds and may cause several cases of death per year in the most severe cases. The present study was planned to evaluate the antibiogram and virulence factors of the isolated bacteria responsible for mastitis. Methods: In this study, 300 milk samples were collected and subjected to identify clinical mastitis (based on clinical symptoms) and sub clinical mastitis (using California mastitis test). E. coli was isolated and identified from mastitis positive samples and further tested for antibiogram and virulence factors such as haemolysis, biofilm formation and fimH and pap genes presence. Result: Out of 300 milk samples collected, 121 (40.33%) were positive for mastitis. E. coli was isolated from only 30 (24.79%) out of 121 mastitis positive samples. On Serotyping for somatic antigen, 13 E. coli isolates belonged to somatic serogroup O83, 2 to O157 and one each to O8, O20, O49, O119, O128 and O145. The highest sensitivity of E. coli isolates was recorded for ciprofloxacin, followed by gentamicin, tetracycline, nitrofurantoin, chloramphenicol, amikacin, cefixime, trimethoprim, ampicillin/sulbactam, ceftriaxone, cefoperazone, cefotaxime, kanamycin, cefotaxime/clavulanic acid and amoxycillin in the decreasing order. Multidrug resistance (MDR) was recorded in 96.66% of isolates. Out of 30 E. coli isolates, 46.66% were haemolytic and 40.0% positive for Congo red dye binding. Biofilm production was shown by 76.66% isolates. Molecular characterization revealed presence of fimH gene in 9 isolates, but pap gene was not detected in any of the strain. It may be concluded that E. coli is an emerging environmental mastitogen in cows and isolation of MDR strains with virulence factors is of serious concern.

Cross-sectional study of cow comfort and management factors associated with subclinical mastitis in smallholder dairy farms in Kenya

A number of environmental and contagious factors have been associated with subclinical mastitis (SCM), which is a common and costly problem for smallholder dairy farmers (SDF). We conducted a cross-sectional study on 118 cows in their first two months post-calving on 109 SDF in Kenya. The study objective was to investigate the relationships among various cow and farm management parameters and SCM specific to SDF. The stall floor comfort level was assessed through knee impact and wetness tests, and cleanliness on the leg and udder were also scored. Various mastitis prevention measures were also assessed (e.g., milking protocols, and use of teat dip and dry cow therapy). Individual quarter SCM was assessed on each cow using California Mastitis Test (CMT). Univariable and multivariable logistic regression models were fit to determine management factors associated with cow-level SCM. Farm-level, cow-level and quarter-level prevalence of SCM was 45.9% (50/109), 43.2% (51/118) and 21.9 % (103/471), respectively. The proportion of stalls scored as dirty was 33.1% while 49.1% of cows had dirty legs. Only 10.1% of farms were using either disinfectant teat dip or dry cow therapy (or both) to prevent mastitis. Low parity and poor stall hygiene were significantly associated with occurrence of SCM. At high daily milk yield, the probability of having SCM was higher in cows housed in a shed with a dirty versus clean alleyway, with no significant difference at low daily milk yield. From the study findings, we concluded that certain cow characteristics and comfort measures were associated with SCM and need to be incorporated in education plans for farmers in SDF.

Clinico-biochemical and therapeutic management of mammitis in buffaloes

Nine graded Murrah buffaloes age between 3 to 6 years old were presented to Veterinary College Hospital, Bidar with the history of recent calving, swelling and reddening of one or two teats. On clinical examination revealed normal body temperature, pain on palpation of one or two teats with subsequent thickening and funnel formation were both in teat canal and gland cistern. Haematological examination revealed mild neutrophilia, marked absolute eosinophilia and lymphopenia. Biochemical examination revealed increased total protein, alkaline phosphatase (ALP), alanine aminotransferase (ALT), Concentration of C - reactive protein and decreased Serum Calcium, Serum Phosphorus. Milk examination of affected animals did not reveal any positive for California Mastitis Test and culture examination. Affected animals were treated with budesonide (200 mcg) aerosol spray along with antihistamines (Chlorpheneramine maleate) 0.3 mg/kg B.wt. Intramuscularly, Antiseptic (Iodine-based teat dip) and Antioxidant (Vit.E and Selenium) 10ml, intramuscularly for 3 consecutive days. It was found that steroid (Budesonide 200 mcg fixed dose), antihistaminic, Iodine based dip application and Vit. E and Selenium were found effective in initial stages to minimise inflammation without any adverse effects.

Multidrug-resistant Bacterial Isolates in Bovine Subclinical Mastitis from Southern Rajasthan

Background: In the present study two hundred milk samples were collected from cows and buffaloes with no history of clinical mastitis in the ongoing lactation, from the Sirohi district of Southern Rajasthan. Methods: The pooled sample (collected from each quarter) and examined for the status of subclinical mastitis by Modified California mastitis test and Somatic cell count respectively. Positive samples were further investigated for isolation and identification of the major mastitis-causing pathogens: S. aureus, predominant Streptococcal species and E. coli for assessing antimicrobial resistance models in southern Rajasthan. Result: The results of the current study indicate high levels of multi-drug antibiotic resistance among bacteria that commonly cause mastitis, particularly ampicillin, penicillin, tetracycline, erythromycin and methicillin. However, the highest sensitivity was conferred to ceftriaxone, gentamicin, and co-trimoxazole, suggestive of judicious use of these antibiotics in the treatment of bovine mastitis. Concurrent implementation of gradient PCR indicated the presence of mecA and blaZ genes in 51.9% and 81.4% of S. aureus isolates, respectively. Meanwhile, 56.6% of the streptococcal isolate contained the tetracycline-conferring tetM gene and none of the streptococci contained the ermB gene. The 92.3% E. coli isolates contained the tetA gene and the tetB gene for tetracycline resistance.

Trends of Occurrence and Therapy of Subclinical Mastitis in Lactating Cows at the Northern Part of Bangladesh

Subclinical mastitis (SCM) causes huge economic losses in the dairy industry due to suppression in milk production. We have conducted this study to see the infection rate of subclinical mastitis in milking cows of Raninagar Upazila and assess the efficacy of intramammary infusion in cows infected by subclinical mastitis. We have taken milk samples from 103 milking cows and screened for SCM using California Mastitis Test (CMT). We recorded the infection rate 51.5 % for SCM and treated the udder with the udder infusion of Mastanil® drug containing Gentamicin sulphate 100mg/ml supplied by Techno Drugs Ltd, Bangladesh. The infection rate was remarkably increased with the age and parity of cows. The prevalence of 33.1% was notably found in the left hind (LH) quarter compared to other quarters. We have also found that the effectiveness of intramammary infusion with Mastanil® drug in lactating cows is 50.9%. Despite limitations regarding accurate disease investigation procedure, we conducted our experiments, thus, further research is warranted to observe the hygiene regarding milker’s hand and milking instruments and dry cow therapy with other potential drug to assess and validate our observations in managing SCM substantially.

Prevalence and Risk Factors of Staphylococcal Subclinical Mastitis in Dairy Animals of Chitwan, Nepal

Subclinical mastitis (SCM), the asymptomatic inflammation of breast tissue, is the most common form of mastitis in livestock. SCM prevalence and risk factors in dairy animals in Western Chitwan, Bagmati Province, Nepal, were the primary objectives of this study. Out of 243 dairy animals, 104 representing 42.8% were positive for SCM in the California mastitis test (CMT) in the study area. At the quarter level, out of 972 active quarters tested for SCM, 188 (19.3%) were positive to CMT test. The prevalence of Staphylococcal SCM was 39.92% (97/243) and 18.21% (177/972) at animal level and quarter level, respectively. Coagulase Negative Staphylococcus (CNS) (46.33%) was the most prevalent Staphylococcus to cause SCM at quarter level. While at animal level, SCM due to occurrence of both S. aureus and CNS (36.08%) in an individual was more common. High susceptibility towards Amikacin, Ceftriaxone and Gentamicin was seen against both isolates. Low resistance against Amikacin and Ceftriaxone was seen against both isolates. Ciprofloxacin (41.2% vs 27.4%) and Gentamicin (37.8% vs 23.2%) were more resistance towards CNS whereas Enrofloxacin (41.1% vs 25.2%) and Tetracycline (36.8% vs 15.1%) were more resistant towards S. aureus. Older aged, multiparous and late lactating animals had the highest prevalence of staphylococcal SCM i.e., 58.1%, 56% and 52.6%, respectively. The study concludes that there is a high prevalence of Staphylococcal SCM in Western Chitwan, Nepal. CNS is the most common mastitis pathogen. Increased antimicrobial resistance to S. aureus and CNS could be the result of the indiscriminate use of antibiotic drugs without an antibiotic susceptibility test (AST). This study emphasizes the importance of ongoing antibiotic surveillance, excellent farm and animal hygiene, and suitable housing and feeding management.

Prevalence of Subclinical Mastitis in Crossbred Cows in Lakhani Tahsil of Bhandara District

Prevalence of subclinical mastitis in total 100 crossbred cows from four different villages around Bhandaracity was studied. A total 400 quarters milk samples from 100 crossbred cows were subjected to ModifiedCalifornia Mastitis Test and Draminski Mastitis Detector, out of which 65 (16.25 %) and 86 (21.5 %) sampleswere found to be infected subclinically respectively. Out of 100 crossbred cow screened, 33 (33.00 %) and 38(38.00 %) cows were found positive for subclinical mastitis by Modified California Mastitis Test andDraminski Mastitis Detector respectively. The cow wise and quarter wise incidence rate of subclinical mastitiswas found 5 per cent more by Draminski Mastitis Detector than Modified California Mastitis Test. Themaximum cow wise prevalence of subclinical mastitis by Modified California Mastitis Test (MCMT) was found in Palandur (40%) followed by Dighori (36%), Kolari (36%) and Kharashi (28%). In respect of quarter wise prevalence percentage by Modified California Mastitis Test, maximum outbreak of the disease was observed in Palandur (18%) and minimum (14%) in Kharashi. Thus, the study indicates that mastitis disease needs priority for its monitoring.

Subclinical Mastitis Detected during the Last Gestation Period Can Increase the Risk of Stillbirth in Dairy Calves.

We hypothesized that subclinical mastitis detected during the last gestation period can increase the risk of stillbirth in dairy calves. The aim was to investigate the relation of subclinical mastitis detected during the last gestation period and its pathogens with the stillbirth of calves. Cows from the 210th day of pregnancy were selected for the study. They were divided into two groups: the first group-subclinical mastitis was confirmed on the farm by the California mastitis test (CMT); the second group of cows-mastitis was not confirmed by the CMT test. Groups of cows were compared according to the results of their calving-the number of stillborn calves. A stillborn calf was defined as a calf that dies at birth or within the first 24 h after calving, following a gestation period of 260 days. Our results suggest that decreasing the incidence of subclinical mastitis during the last gestation period (from the 210th day of pregnancy) can decrease the risk of stillbirth in dairy calves. Further, it is important to identify the pathogen because the highest risk of stillbirth was found in cows with mastitis caused by Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, pathogenic Staphylococci and other Streptococci. Cows at the first calving had a 1.38-1.65-times higher risk of having stillborn calves than cows of parity ≥ 2. From a practical point, veterinarians and farmers can consider the effect of subclinical mastitis during late gestation on the risk of stillbirth and it could help for strategies of optimizing reproductive performance in dairy cows.

Prevalence of subclinical mastitis, associated risk factors and antimicrobial susceptibility of the pathogens isolated from milk samples of dairy cows in Northwest Ethiopia

Subclinical mastitis (SCM) is a problem of dairy cattle worldwide that affects the income of dairy farmers and besides a health risk to humans. A cross-sectional study was carried out to estimate prevalence, identify risk factors of SCM and antimicrobial susceptibility test of bacteria isolated from SCM quarters. California Mastitis Test (CMT) positive milk samples were cultured and bacteria isolated. The isolated bacteria were checked for their antimicrobial susceptibility using Kirby-Bauer disk diffusion method. The study findings showed that 32.7% of the quarters and 60.1% of the cows were CMT positive. The isolated bacteria were 29.5% Staphylococcus aureus (S. aureus) and 9.3% Streptococcus agalactiae (Str. agalactae). In the risk factors study, cows from farms of poor hygiene, increased age and stage of lactation had higher odds of SCM. Of the isolated bacterial species, 75.7% and 71.4% of S. aureus isolates were resistant to the effects of penicillin and ampicillin, respectively. Streptococcus agalactiae were resistant to the effects of oxytetracycline (76.9%), streptomycin (61.5%) and cloxacillin (53.8%). Whereas the majority of S. aureus (98.6%) and Str. agalactiae (88.5%) were susceptible to the effects of gentamycin and Clindamycin respectively. In conclusion, SCM had high level of prevalence and associated risk factors with pathogens that were resistant to commonly used antimicrobials. Implementing strict preventive measures and limiting spread of pathogens is a preferred approach rather than treatment with antibiotics to control mastitis in the study area.

Ocorrência de leite instável não ácido (LINA) em propriedades leiteiras no norte do Tocantins

The diagnosis of unstable non-acid milk (UNAM) in Brazil is overlooked due to the lack of information about this problem available to dairy farmers, mainly in the North region of the country. This report describes the occurrence of UNAM in three dairy farms of family farmers in northern Tocantins state, Brazil, who use animal feed based on Urochloa brizantha without mineral supplementation. The animals had been subjected to a poor feeding practice, with low quantity and quality, due to the dry period in the region and inappropriate nutritional management. Fourteen animals that presented alterations in milk characteristics were subjected to 72% alizarol test, California mastitis test, strip cup test, somatic cell count (SCC), boil test, physical-chemical and microbiological analyses, and search for undesirable substances. The time of lactation, udder health, and serum glucose level of animals, and soil and forage quality were also evaluated. The results showed 42% animals diagnosed with mastitis, 21% of them with clinical and 21% with subclinical mastitis; and 78% of animals with samples positive for the alizarol and boil tests, and Dornic acidity between 14° and 18°, characterizing positive cases for UNAM. Only one animal presented SCC above the limit established by the Brazilian legislation. The animals presented undamaged teats and no alteration in glycemia. Changes in nutritional management were recommended and done and the alizarol tests were redone after 14 days, showing all negative results for UNAM. The conclusion is that incorrect nutritional management is the main cause of UNAN in the studied cases.

Aspectos econômicos e produtivos do manejo de ordenha na pecuária leiteira

The Brazilian dairy sector is important for the world agricultural production; however, it presents problems in production efficiency and milk quality, since the occurrence of mastitis in the herds can reach 47%. The present study analyzed data from a rural property with 100 lactating cows in a semi-intensive breeding system, which did not adopt adequate hygiene management for milking processes. Corrective measures were proposed, such as daily strip cup test, biweekly California Mastitis Test, formation of a new milking line, and daily pre dipping and post dipping and cleaning of teat cups. Milk samples from 10 animals with positive clinical mastitis, 10 animals with positive subclinical mastitis, and from the cooling tank were collected at one day before and 90 days after the implementation of the proposed measures and sent to a laboratory for analysis of somatic cell counting (SCC) and total bacteria counting (TBC). An increase of 17% in milk production and decreases of 64% and 40% in clinical and subclinical mastitis, respectively, were found after 90 days, with a consequent decrease in SCC and TBC. The milking duration after this period denoted an adaptation of the employees to the new procedures. The production financial yield was calculated based on the implementation costs of the adopted adequations and increases in milk production, representing a profitability increase of 10.4%. The implemented adequations were efficient for the control of mastitis and increase in milk yield and quality, resulting in a positive economic return.

Subclinical bovine mastitis associated with Staphylococcus spp. in eleven Uruguayan dairy farms.

Bovine mastitis is the most common disease affecting the dairy industry, with staphylococci being considered as one of the most significant and prevalent causes. This study aimed to assess the presence of staphylococcal subclinical mastitis (SCM) in Uruguayan dairy farms and to identify Staphylococcus aureus (SA) and non-aureus staphylococci (NAS) in milking cows. In addition, the antibiotic susceptibility of isolated staphylococci was evaluated. We tested 546 apparently healthy milking cows from 11 farms for detecting SCM using the California Mastitis Test (CMT). The cows were not treated with antibiotics. CMT-positive samples were cultured, and colonies compatible with Staphylococcus spp. were further identified through molecular techniques. The susceptibility of the Staphylococcus spp. isolates against thirteen antibiotics was determined using the disk diffusion method. Subclinical staphylococcal mastitis was present in almost all (82%) farms. SA (n = 39) was more common than NAS (n = 9) in the 48 samples tested. Isolates exhibited resistance to one, two, and even three different antibiotics. Resistance to penicillin was the most frequent among SA (23/39) and NAS (4/9). No staphylococci isolates exhibited resistance to cefoxitin, vancomycin, trimethoprim-sulfamethoxazole, erythromycin, or clindamycin. Staphylococcal SCM is one of the most common diseases in Uruguayan dairy farms. SA was the prevalent pathogen, however SA and NAS mastitis coexisted in many farms. NAS were identified and its distribution was similar to other countries. Penicillin had the highest and most frequent percentage of resistance.

Use of Multivariate Adaptive Regression Splines, Classification Tree and Roc Curve in Diagnosis of Subclinical Mastitis in Dairy Cattle

Subclinical mastitis is one of the most significant diseases that cause economic losses in dairy cattle farming. This investigation was conducted on 112 head Holstein Friesian cows in order to reveal relationship between subclinical mastitis and electrical conductivity milk composition and milk quality. In the study, CMT (California Mastitis Test) and CSCC (Classified Somatic Cell Count) used in diagnosis of subclinical mastitis were used as a binary response variable i.e. healthy and unhealthy. Potential predictors included here were lactation number, days in milk (DIM), L, a, b, H, C, milk fat, milk protein, lactose, milk freezing point, SNF, density, solids, pH and electrical conductivity. CART, CHAID, Exhaustive CHAID, QUEST and multivariate adaptive regression splines (MARS) were used as data mining algorithms that help to make an accurate decision about detecting influential factors increasing risk of subclinical mastitis.In conclusion, better classification performances of CART and MARS data mining algorithms were determined compared with those of remaining algorithms in order to correctly discriminate healthy and unhealthy cows.

The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi

Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.

Milk Immune Cell Composition in Dromedary Camels With Subclinical Mastitis.

Mastitis represents one of the most important infectious diseases in camels with heavy economic losses due to reduced milk quantity and quality. Balanced immune cell composition and function in the mammary gland are essential for effective immune response to mastitis pathogens. The objective of the present study was to characterize the cellular immune response to subclinical mastitis in the mammary gland of dromedary camels. Therefore, immunostaining and flow cytometry were used to compare the cellular composition, leukocyte phenotype, and cell viability in camel milk from healthy she-camels (n = 8) and she-camels with subclinical mastitis (SCM; n = 6). In addition, the ex vivo phagocytic activity of milk phagocytes was compared between healthy and affected animals. The health status of the mammary gland was evaluated based on the California Mastitis Test (CMT) score. SCM (CMT score of ≥3 in the absence of clinical signs of mastitis) was found in six of the 56 sampled quarters (10.7 %) with only one affected quarter per animal. In comparison to milk from healthy camels, milk from SCM animals showed higher somatic cell count (SCC), higher numbers of CD45+ leukocytes with an expanded fraction of CD172a+ myeloid cells. Within the myeloid cell population, there was an increase in the percentage of granulocytes (CD172a+CD14low) with a decreased percentage of macrophages (CD172a+CD14high) in milk from affected animals compared to healthy animals. The decrease in lymphoid cells in SCM milk was mainly due to the decreased fraction of CD4+ helper T cells. Camel SCM was also associated with a stimulated phenotype, increased cell viability, and enhanced phagocytic activity of the milk phagocytes, macrophages and granulocytes. Collectively, the present study identified significant changes in SCC, leukocyte count, phenotype, viability, and function in association with subclinical mastitis in camels. The results of the present study support a better understanding of host-pathogen interaction mechanisms in the camel mammary gland.

The Empowerment of Livestock Farmers in Subclinical Mastitis Test with GAMA Anti-Haptoglobin in “Sahabat Ternak” Etawah Crossbreed Goat Farm

Sahabat Ternak is one of the Etawah crossbreed (PE) goat farm groups in Sleman. This farm group focuses on goat milk production and processed goods. Problems that commonly arise in dairy goat farms are the cases of subclinical mastitis, which are quite high. This disease may cause a decrease in milk production and quality. The mastitis subclinical detection method which is often used by the farming community is the somatic cell count (SCC) and California mastitis test (CMT). However, both tests have low accuracy. Recently, a new method named GAMA Anti-Haptoglobin, which is more accurate and can be done easily has been established by livestock farmers. This community service aims to empower livestock farmers in applying GAMA Anti-Haptoglobin as a sensitive, rapid, and accurate subclinical mastitis detection kit in Sahabat Ternak goat farm. The method used in this activity consisted of discussion, socialization, and training for livestock farmers, as well as laboratory testing, evaluation of test findings, and treatment for PE goats. After the training, the livestock farmers were able to apply GAMA Anti-Haptoglobin mastitis detection method effectively. The implementation of this easy and accurate field mastitis detection method, as well as personnel with reliable skills, will support in the decrease of mastitis cases and increase milk production and quality, as well as community welfare.