AbstractRecent methods using thermistors as an early detection of skin cancer require the higher resolution of 5 mK. Previously, the authors developed a vacuum insulated temperature device that calibrates the resistance temperature characteristics of thermistors, so that it improves the temperature resolution and accuracy of skin cancer diagnosis. In this study, the operational methods of the calibration system were compared for improving further accuracy using the temperature calibration device and the oil tank calibration device. The results of the comparison indicated that the temperature calibration device is probably as accurate as the oil tank calibration device.