Calf Scour Research Articles

Development of a Highly Sensitive Electrochemical Detection of Salivary Glucose and pH Sensors Based on Glucose Oxidase Using Interdigitated Microelectrodes

The use of electrochemical nanosensors has seen a significant uptick in recent years, largely due to the improved chemical and physical properties that result from the modification of nano or microelectrode devices with nanomaterials.[1] These electrodes play a crucial role in enhancing electrochemical-based nanosensors, offering benefits such as a higher signal-to-noise ratio, lower detection limits, cost-effectiveness, ease of fabrication, and the ability to achieve steady-state currents. The interest in electrochemical nanosensors is considerable, given their broad application spectrum, which includes areas like animal health, human health, environmental monitoring, and bioprocess monitoring.[2] The focus of this research is the development of on-chip salivary glucose and pH sensors that are integrated on silicon wafers. These sensors are specifically designed to identify two frequently occurring bovine diseases: bovine respiratory disease (BRD) and scour. Both of these diseases are highly contagious, often resulting in fatal outcomes for calves, and cause economic losses amounting to millions of euros across Europe. Therefore, early detection of BRD and scour is essential to prevent, oversee, or delay the progression of the disease and its related complications. However, the current methods of blood glucose testing for calves have a significant disadvantage, as they require regular invasive blood sampling. Encouragingly, the analysis of saliva has surfaced as a non-invasive and convenient alternative for monitoring glucose levels in calves with above-mentioned disease.[3] Thus, it is necessary to develop a highly sensitive electrochemical nanosensor for early detection of BRD and scour by using biomarkers (glucose, pH) in a calf's saliva. The proposed electrochemical nanosensor is based on a micro fabricated device that integrates a silicon microelectrode, reference electrode, and counter electrode with six individual sensor that can be used for the detection of six different biomarkers i.e. glucose, pH, lactate, electrolytes, etc. The sensing mechanism is based on the measurement of changes in current that occur because of glucose and pH interactions with the modified silicon surface. The sensors are designed to detect small changes in glucose and pH concentrations that can be indicative of the onset of BRD and scour. The main advantage of the proposed electrochemical nanosensor is its ability to provide rapid and accurate detection of BRD and scour in calves. Early detection of these diseases can significantly improve treatment outcomes and reduce the risk of transmission to other animals. In addition, the low cost and ease of fabrication of these sensors make them an attractive option for large-scale deployment in agricultural settings. In conclusion, the development of on-chip electrochemical nanosensors for the early detection of bovine respiratory disease and scour by using saliva as a medium is a promising approach for improving animal health and reducing economic losses. The proposed sensors offer high sensitivity, low cost, and easy fabrication, making them an attractive option for widespread deployment in agricultural settings. Future research can focus on optimizing the sensor design for improved performance and expanding the range of biomarkers that can be detected.This work represents the microfabrication and characterization of gold interdigitated electrodes (IDE) on silicon using standard microfabrication methods i.e., lithography and etching techniques. The sensor have six individual working electrodes (WEs) along with on-chip gold counter and platinum reference electrodes and can be modified individually for glucose and pH sensor. A two-step electrodeposition process was carried out for platinum platting thus incorporating glucose oxidase (GOx) onto a platinum-modified gold IDE with an o-phenylenediamine (o-PD). The enzymatic based nanosensor demonstrated a linear reduction response to wider range of glucose from 0.02-7mM using chronoamperometric measurements in artificial saliva (Figure 1a,b). In addition, real calf samples have been used to detect the glucose level in calf saliva and compared with commercial glucometer (Figure 1c). Furthermore, at gold IDE, the sensor exhibits linear responses for pH ranging from pH 5-8 using cyclic voltammetric analysis (Figure 1d,f).

Comparative Studies on Supportive Treatment of Calf Scour

Comparative Studies on Supportive Treatment of Calf Scour

Genetic characterization and clinicopathological features of <em>Escherichia coli</em> in neonatal septicemic calves

Due to severe diarrhea associated with calf scour and septicemia, colibacillosis is one of the leading causes of neonatal mortality in calves. This study investigated the clinical and hematological manifestations as well as the genetic composition of Shiga toxin-producing E. coli (STEC) strains in septicemic neonatal calves. In this study, 47 fecal specimens were collected from diarrheic calves aged between one and three weeks on dairy farms that are unorganized around Basra, Iraq, while twenty age-matched calves were tested as controls. Of the 47 tested samples, 28 fecal samples were tentatively identified as STEC, with a prevalence of 60%. STEC-affected calves had variable rectal temperatures ranging from subnormal to fever, tachycardia, hyperpnea, diarrhea, partial or complete cessation of suckling, mucous membrane congestion, depression, incoordination and rough hair coat conditions. Significant changes were observed in the hemogram and the leukogram during the analysis of blood samples from calves with diarrhea. Molecular analysis of fecal samples demonstrated that local STEC isolates are more closely related to other strains obtained from both human and animal samples worldwide. Based on the results of the current study, we can conclude that the septicemic calves were likely infected by human or environmental resources and could play a major role in further spreading STEC infection.

Specific pathway abundances in the neonatal calf faecal microbiome are associated with susceptibility to Cryptosporidium parvum infection: a metagenomic analysis

BackgroundCryptosporidium parvum is the main cause of calf scour worldwide. With limited therapeutic options and research compared to other Apicomplexa, it is important to understand the parasites’ biology and interactions with the host and microbiome in order to develop novel strategies against this infection. The age-dependent nature of symptomatic cryptosporidiosis suggests a link to the undeveloped immune response, the immature intestinal epithelium, and its associated microbiota. This led us to hypothesise that specific features of the early life microbiome could predict calf susceptibility to C. parvum infection.ResultsIn this study, a single faecal swab sample was collected from each calf within the first week of life in a cohort of 346 animals. All 346 calves were subsequently monitored for clinical signs of cryptosporidiosis, and calves that developed diarrhoea were tested for Rotavirus, Coronavirus, E. coli F5 (K99) and C. parvum by lateral flow test (LFT). A retrospective case–control approach was taken whereby a subset of healthy calves (Control group; n = 33) and calves that went on to develop clinical signs of infectious diarrhoea and test positive for C. parvum infection via LFT (Cryptosporidium-positive group; n = 32) were selected from this cohort, five of which were excluded due to low DNA quality. A metagenomic analysis was conducted on the faecal microbiomes of the control group (n = 30) and the Cryptosporidium-positive group (n = 30) prior to infection, to determine features predictive of cryptosporidiosis. Taxonomic analysis showed no significant differences in alpha diversity, beta diversity, and taxa relative abundance between controls and Cryptosporidium-positive groups. Analysis of functional potential showed pathways related to isoprenoid precursor, haem and purine biosynthesis were significantly higher in abundance in calves that later tested positive for C. parvum (q ≤ 0.25). These pathways are either absent or streamlined in the C. parvum parasites. Though the de novo production of isoprenoid precursors, haem and purines are absent, C. parvum has been shown to encode enzymes that catalyse the downstream reactions of these pathway metabolites, indicating that C. parvum may scavenge those products from an external source.ConclusionsThe host has previously been put forward as the source of essential metabolites, but our study suggests that C. parvum may also be able to harness specific metabolic pathways of the microbiota in order to survive and replicate. This finding is important as components of these microbial pathways could be exploited as potential therapeutic targets for the prevention or mitigation of cryptosporidiosis in bovine neonates.

Vaccine use in Canadian cow-calf herds and opportunities for improvement.

Vaccinations are one of the most impactful tools available to cow-calf producers to control within herd disease and later, in feedlots. While vaccine use has been studied across Canada, inconsistent and variable regional data makes analysis and interpretation difficult. The objective of this study was to describe vaccination protocols and factors associated with vaccine use in Canadian cow-calf herds and define associations between vaccine use and productivity outcomes. Surveys describing vaccine use in 2020 were collected from 131 cow-calf herds (40 eastern, 91 western), recruited through a national beef cattle surveillance program. Ninety-two percent of cows and replacement heifers, and 72% of bulls were vaccinated with Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea Virus (BVDV), Parainfluenza3 Virus (PI3), and Bovine Respiratory Syncytial Virus (BRSV). At least half of cows and bulls were vaccinated for clostridial pathogens and cows and heifers for viral calf scours. Clostridial vaccines were significantly more likely to be used in western Canada compared to eastern Canada. While 92% of producers vaccinated suckling calves against IBR/BRSV/PI3, only 47% provided a second vaccine prior to weaning; 78% of calves were also vaccinated at least once for BVDV before weaning. Producers who vaccinated calves against IBR/BRSV/PI3 before 3 months of age provided a second dose prior to weaning more often than producers who administer the first IBR/BRSV/PI3 vaccine later. Vaccine use has increased across Canada, particularly in calves, prior to weaning. Relative to label recommendations for annual vaccination, clostridial vaccines were generally underutilized in cows and bulls, and by producers in eastern Canada as compared to western Canada. Opportunities also exist to improve adherence to label recommendations for the booster dose of scours vaccine when used in bred replacement heifers. Protocols including product choices, the timing and boosting of respiratory vaccines in nursing calves vary widely across herds. Use of intranasal vaccines in neonatal calves less than 2 weeks old has increased in western Canada compared to previous reports. There is a need to better understand how timing of vaccination in nursing calves contributes to effectiveness, for respiratory disease in nursing and weaned calves.

Effect of vitamin E and zinc supplementation in pre and post partum period incrossbred cows on anti-oxidant, immunity status and performance of new borncalves

The present study was undertaken to investigate the effect of zinc (Zn) supplementation alone and its combination with vitamin E on growth, anti oxidant status and immunity status of new born calves of crossbred cows. The cows were supplemented with Zn @ 0 ppm (T0), Zn @ 80 ppm (T1) and Zn @80 ppm + 1000 IU α-tocopheryl acetate/d (T2) from 60 days prepartum up to 90 days postpartum. The fat, SNF, total solid, protein, Zn and Cu contents content of colostrum showed no effect of dietary treatments but α-tocopherol content was higher(P<0.01) in group T2 as compared to other groups. Total immunoglobulin in colostrum of T2 group was 25.87% higher than control. There was no effect of dietary treatments on plasma Zn and Cu levels of calves, however α-tocopherol concentration at day 5 was higher (P<0.05) in group T2 as compared to other groups. Status of antioxidant enzymes, lymphocyte proliferation index,total and differential leukocyte count was not affected by the treatments. Higher birth weight (P<0.05) was obtained in groupT2 group as compared to other group. Calves from treatment groups showed lower incidence of morbidity and mortality due to calf scour and other infectious diseases compared to control

Assessment of Calf Management and Hygiene Practices Adopted in Large and Small-Scale Dairy Farms in Wondo Genet Area, Southern Ethiopia.

Although calf management is the cornerstone of dairy cattle productivity and profitability, little attention is given by researchers particularly in developing nations including Ethiopia. Therefore, this research was conducted to characterize calf management and hygiene practices adopted by dairy farmers at Wondo Genet, a potential milk shed for southern Ethiopia. Calf management and hygiene practice-related data were collected from 57 dairy farms through a semi-structured pre-tested questionnaire and personal observation. All sampled farms were visited once by technical staff and administered the questionnaire by face-to-face interview during the period February 2017 to December 2019. The obtained data were analyzed using descriptive statistics. The majority (96.5%) of the farms had a slatted calf pen floor that was made of concrete. Although more than half of the farms (57.9%) had a good calf pen drainage system, only some (n = 8, 14%) are cleaned on a daily basis. All the calves (100%) had a history of calf scour, 96% survived from cowdriosis (heartwater). The majority of the calves born in the dairy farms (68.5%) received colostrum within 30 minutes of birth, but most of them (82.5%) were fed with waste milk till weaning age. Although the majority of the farms separately house recently born calves, 24.6% of the farms raise their calves together with other domestic animals. Dairy farm owners in the study area adopted some established risky calf management and hygiene practices that might lead to high calf morbidity/mortality in the farm. Awareness creation and further study to identify the specific causes of mortality and morbidity should be in place to improve the management and hygiene of calves and implement specific control and preventive measures.

New licence claim for calf scour vaccine

Veterinary RecordVolume 191, Issue 12 p. 490-490 Business & Innovation New licence claim for calf scour vaccine First published: 16 December 2022 https://doi.org/10.1002/vetr.2554Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume191, Issue1217–31 December 2022Pages 490-490 RelatedInformation

Vaccine to prevent calf scour

Veterinary RecordVolume 191, Issue 10 p. 408-408 Business & Innovation Vaccine to prevent calf scour First published: 18 November 2022 https://doi.org/10.1002/vetr.2451Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume191, Issue1019/26 November 2022Pages 408-408 RelatedInformation

Youngstock health: a focus on Mycoplasma bovis, nematode control and the use of NSAIDS in scour

Foreword Farming is under increasing scrutiny on environmental issues (including greenhouse gas emissions, resource use, water and air quality, biodiversity and other impacts), in addition to increasing economic and social pressures. It is increasingly clear that we need to ensure that every animal has a good healthy life, while reducing losses throughout the production system. As cattle vets, we work with cattle at every stage of the production cycle, but on many dairy farms, youngstock are still seen as being of secondary importance compared with the mature herd, suffering significant levels of disease, and sustaining high mortality. This is despite the fact that dairy replacements are, or should be, the group with the highest genetic merit on the farm and are the future of the herd. In contrast to the dairy herd, calves from the suckler herd are the only saleable product of the cow and thus her whole production for the year is lost if the calf should die. Despite this, more could still be done on many farms to improve health and productivity of these calves allowing them to be finished faster with associated environmental benefits. For environmental, economic and social reasons, it is no longer acceptable to cull dairy bull calves, or to ignore high levels of morbidity or mortality. An animal that has a suboptimal growth rate or dies represents a financial loss to the farmer, a welfare concern to the consumer, and an environmental impact that is not compensated for by food (milk or meat) production. If we are to serve our clients to the best of our ability and help make their businesses as sustainable as possible, we need a greater focus on youngstock. This supplement is a good place to start with its focus on Mycoplasma bovis, nematode control and the use of NSAIDs in calf scour.

Etiological and Pathomorphological Investigations of Coronavirus and Rotavirus Gastroenteritis in Goat Kids and Lambs

Abstract The aim of the performed field and laboratory investigations was to determine the commonest enteropathogens in neonatal and juvenile lambs and goat kids, as well as induced macro- and microscopic alterations in the different digestive system compartments. The study comprised a total of 850 newborn and juvenile lambs (600) and goat kids (250) from 9 private dairy farms (3 goat farms, 6 sheep farms). The age of animals was from 24 hours to 20 days. The following rapid antigen detection tests were used: (Rainbow calf scour 5 BIO K 306, Monoclonal Antibody anti-Coronavirus аnd Rotavirus FITC conjugated, BIOX Diagnostics, Belgium). Gross anatomy and histopathological examination of tissue samples was performed on 21 carcasses (9 goat kids and 12 lambs) from animals that died with signs of gastroenteritis. The main detected agents of intestinal infections were Cryptosporidium parvum, rotaviruses, coronaviruses and Escherichia coli.

Calf scour management and alternative therapies

Prevention is better than cure undoubtedly, but allied to that is a new approach to the types of products that should be considered in the light of current antimicrobial resistance issues and the heightened importance of calf immunity.

Adoption gap of scientific management practices among the commercial dairy farmers of Punjab

Result revealed that although of the respondents fell under low and medium adoption gap categories for most of the scientific management practices but still major adoption gap percentage was found in health care (47.4%), feeding management (42.9%) and calf management (31%). The health care practices which were not followed included screening of herd for mastitis and tuberculosis. Medium gap in feeding practices was observed in silage making, processing of feed and UMMB feeding. Practice of calf scour management was sometimes followed by majority of the respondents. Other practices which were never followed by majority of the respondents include practice of availability of foot dips at entrance, feeding milk replacer to calves, milking animals in milk barn and insurance of animals. State animal husbandry department, dairy cooperatives and farmers organisation working in those areas should disseminate knowledge about fodder preservation, health care and hygiene of animals as well as clean milk production which in return would prevent them from incurring economic losses due to disease outbreak and fetching them high price for the clean milk produced.

Cryptosporidium parvum and other enteric pathogens in scouring neonatal dairy calves from the Al Ain region, United Arab Emirates.

Faecal specimens from 36 scouring neonatal calves from two dairy farms located in the Al Ain region of the UAE were screened with pathogen-specific antigen ELISA for Cryptosporidium parvum, Escherichia coli K99, rotavirus, and coronavirus. Additionally, faecal smears were stained with modified-acid-fast for Cryptosporidium oocysts, and the VITEK 2 system plus Gram's stain used to identify bacteria isolated from the faecal samples. Farm management practices were also evaluated during a farm visit. Of the 36 calves, 29, 13, 5, and 6 were positive for C. parvum, E. coli K99, bovine coronavirus, and rotavirus antigens respectively, while 27 were positive for Cryptosporidium oocysts. In various combinations, mixed infections were detected in 20/36 calves. This is the first report of C. parvum, E. coli K99, Salmonella spp., rotavirus, and coronavirus in ≤14-days-old scouring neonatal dairy calves from the UAE. Molecular characterization of these pathogens and nationwide epidemiological calf scour studies are recommended.

Investigation of Rota And Corona Viruses as Causative Agents for Diarrhea in Egyptian Calves

Rota and Corona viruses are the major causes of calf scour involved in 27% to 36% of calf diarrheic in Egypt. This study was aimed to investigate frequency of Rota and Corona viruses in diarrheic cattle and buffalo calves in Egypt. A total of 82 fecal samples were collected from 0-1-month-old in three provinces in Egypt (Ismailia, Alexandria and Al-Sharqia). All fecal samples were tested by probe based real time polymerase chain reaction technique (qRT-PCR) to determine the presence of BRV and BCoV nucleic acids by using specific generic primers. Fourteen (17.1%) and 22 (26.8%) out of 82 fecal samples were positive for BRV and BCoV, respectively. Positivity percentage of different investigation parameters about both BRV and BCoV were calculated depending on our historical data. Corona and Rota viruses' infection in cattle were more predominant than buffalo calves. Infection rate of Coronavirus is more than Rotavirus infection in cattle calves of Ismailia than other studied provinces. Single infection of cattle or buffalo calves with both viruses is mostly predominant but double infection in the same animal species at the same time is possible. Infection rate of BRV and BCoV in cattle and buffalo calves is higher in winter than autumn, summer and spring.

Etiological and Pathomorphological Investigations in Calves with Coronaviral Pneumoenteritis

Abstract The aim of the present study was to report the primary gross and microscopic lesions, as well as etiological agents of field cases of pneumoenteritis in neonate and juvenile calves. The research was done with 370 calves from 6 cattle farms in 4 regions of the country. The age of the animals was from 24 hours to 25 days. Clinical and epidemiological studies were carried out with newborn and growing calves in all farms. For rapid antigenic and viral detection of pathogens, Rainbow calf scour 5 BIO K 306 Detection of Rota, Corona, E.coli F5, Crypto and Clostridium perf. in bovine stool (BIOX Diagnostics, Belgium), and Monoclonal Antibody anti-bovine Coronavirus FITC conjugated) 0,5 ml (20X), BIO 023, (BIOX Diagnostics, Belgium) were used. Eighteen carcasses of calves with signs of pneumoenteritis syndrome (PES) were submitted to gross anatomy and histopathological studies. Bovine coronavirus (BCoV) was the main etiological agent involved in calf pneumoenteritis. The macro- and micro lesions in the lung and the ileum of calves affected by PES are relevant with regard to the differential diagnosis of the syndrome and its differentiation from respiratory (IBR, BVD, BRSV, M. haemolytica etc.) and intestinal (Cryptosporidium parvum, bovine rotaviruses, bovine coronaviruses and Escherichia coli K99 (F5) diseases in this category of animals.

Genomic evaluation for calf wellness traits in Holstein cattle

Reducing calf morbidity and mortality is important for attaining financial sustainability and improving animal welfare on commercial dairy operations. Zoetis (Kalamazoo, MI) has developed genomic predictions for calf wellness traits in Holsteins that include calf respiratory disease (RESP; recorded between 0 and 365 d of age), calf scours (DIAR; recorded between 2 and 50 d of age), and calf livability (DEAD; recorded between 2 and 365 d of age). Phenotype and pedigree data were from commercial dairies and provided directly by producers upon obtaining their permission. The number of records ranged from 741,484 for DIAR to 1,926,261 for DEAD. The number of genotyped animals was 325,025. All traits were analyzed using a univariate threshold animal model including fixed effect of year of birth × calving season × region, and random effects of herd × year of birth and animal. A total of 45,425 SNP were used in genomic analyses. Animals genotyped with low-density chips were imputed to the required number of SNP. All analyses were conducted using single-step genomic BLUP implementing the "algorithm for proven and young" (APY) animals designed to accommodate very large numbers of genotypes. Estimated heritabilities were 0.042, 0.045, and 0.060 for RESP, DIAR, and DEAD, respectively. The genomic predicted transmitting abilities ranged between -8.0 and 24.0, -11.5 and 28.5, and -6.5 to 22.8 for RESP, DIAR, and DEAD, respectively. Reliabilities of breeding values were obtained by approximation based on partitioning of a function of reliability into contributions from records, pedigree, and genotypes, where the genotype contribution was approximated using the diagonal value of the genomic relationship matrix. The average reliabilities for the genotyped animals were 41.9, 42.6, and 47.3% for RESP, DIAR, and DEAD, respectively. Estimated genomic predicted transmitting abilities and reliabilities were approximately normally distributed for all analyzed traits. Approximated genetic correlations of calf wellness with Zoetis dairy wellness traits and traits included in the US national genetic evaluation were low to moderate. The results indicate that direct evaluation of calf wellness traits under a genomic threshold model is feasible and offers predictions with average reliabilities comparable to other lowly heritable traits. Genetic selection for calf wellness traits presents a compelling opportunity for dairy producers to help manage herd replacement costs and improve overall profitability.

Impact of milk feeding frequency on performance, health and behaviour of crossbred cattle calves

The present study was undertaken to study the impact of milk feeding frequency on performance, health and behaviour of crossbred cattle calves. A total of 12 crossbred (Vrindavani) calves were allocated equally in to two groups viz. control (G-I) and experimental group (G-II). During the experimental period (from16th to 70th day of birth), the calves in Gr-II and G-I group were fed milk once and twice daily, respectively without any change in total offered milk quantity. The mean birth weight (kg) of calves in control and experimental group were 21.01±0.88 and 21.25±0.88 kg, respectively. The total body weight gain of calves in control and experimental group were 21.06±0.39 and 21.08±0.39 kg, respectively. The average weekly body weights, gains and body measurements in general were non-significantly different in control and G-II group from 1st to 10th week of age. However, the body length was significantly (P<0.05) higher in G-II from third week to end of experiment. As a whole, 16.67% calves in G-I and 33.34% calves in G-II suffered from calf scour. The change in feeding schedule had no significant effect on immune status and behavioral activities of calves. Once daily feeding saved 29.41% (36 man minutes) labour than twice daily feeding schedule. It was concluded that reduction in milk feeding frequency to calves did not affect growth, feed intake, health, behaviour and overall performance, hence one time milk feeding to calves may be adopted at organized herds, where weaning is being practiced, in view of saving labor cost.

Growth performance of yearling F1 progeny of different crossbred beef cattle

Bangladesh has a great demand of beef but it has been facing demand and supply mismatches due to insufficient production and supply of beef, low carcass yield of native cattle and recent no-cattle export policy of a long bordered neighboring country. Thus, the present work was undertaken with an objective to develop market beef cattle of average >150 Kg carcass weight by 24 months with an average FCR of <6.50 under on farm feeding and management condition. Aiming at developing breeding bulls the native dams of BCB-1 (BLRI Cattle Breed 1) were inseminated with the imported frozen semen of Brahman, Simmental, Charolais and Limousin. The crossbred progeny of different assorted F1 genotypes were being selected and their production and breeding performance were evaluated and compared with BCB-1. All pregnant (> 6 months of gestation period) cows were in pre-natal care, and all calves were raised in a single plane of nutrition and management. All crossbred progeny performed better than BCB-1 in terms of live weights and average daily gains. Among the crossbreds, Charolais×BCB-1 had the highest (p<0.001) birth weight (27.5±1.52 kg) followed by Brahman×BCB-1 (24.1±1.23), Simmental×BCB-1 (21.9±1.78), Limousin×BCB-1 (19.8±1.39) and BCB-1×BCB-1 (18.4±1.09), and the genotypes differences were highly significant (p<0.001). In average growth curve, Simmental cross grew faster followed by Charolais, Limousin, Brahman crosses and purebred BCB-1. The genotype×environment interaction was only observed in birth weight. Purebred BCB-1 had the lowest daily DM intake and showed the lowest FCR in all ages compare to other crosses. Calf scour and alopecia occurred in all genotypes. In this breeding program, calf mortality was found as 5.26%. Simmental×BCB-1 is performing as the best among the five genotypes in terms of growth up to 12 month of age.Bang. J. Anim. Sci. 2017. 46 (2): 82-87

Clinico-Pathological Findings of Septicaemic Colibacillosis in a Calf

Colibacillosis is one of the major cause of mortality among young calves due to severe diarrhoea associated with calf scour and septicaemia. A two weeks old male Friesian cross calf weighing about 50 kg was presented to the large animal clinic of the University Veterinary Hospital with complains of yellowish-watery diarrhoea for the past two days, lethergy and recumbency. The calf was dull and depressed, diarrhoeic, dehydrated with bilateral sunken and congested eyes. Three other calves had recently died of recurring similar symptoms in the same herd. Antibiotic and fluid therapy with analgesic as adjunct treatment plan, but the calf died while being treated.The blood biochemical analysis revealed hyperkalaemia, hypocalcaemia, slightly elevated urea, creatinine and aspartate aminotransferase (AST). Necropsy findings were congestion and haemorrhage in the abomasum, jejunum and colon with sloughing off of the mucosa. The liver was pale, kidneys and heart were congested and haemorrhagic. Bacteriological examination revealed that E. coli was consistently isolated in biopsy samples of the visceral organs. Improved sanitation and provision of adequate colostrum to new-born calves have forestalled the occurrence of the disease in the herd.