Aim. Investigate competition and selection material of tobacco for resistance to major diseases for immunological characterization of samples and use of results in practical selection.General scientific and special work methods: field, laboratory, selection that use induction apomixis, calculation, mathematical and statistical. The use of apomixis in tobacco breeding helps shorten the selection process by 4–6 years. During the assessment of the selection material for the resistance of tobacco to harmful organisms, we have used the results substantiated by us during 2011−2015 scales for assessing the intensity of damage by the most common and harmful diseases and the settlement and damage by pests. Results. Tobacco varieties and hybrids with individual and complex resistance to diseases, that are common in the Transnistria region of Ukraine, were selected for the purpose of using them in selection work. On the basis of these samples, a characteristic collection of tobacco was created and registered on resistance to biotic and abiotic factors.Conclusions. During the years of research (2016−2020), varieties and hybrids of tobacco were selected in competitive and selective variety tests (Ternopilsky 7, Berley 38, Berley 46, Ukrainsky Novy, Fn Banat × Ukrainian new, An Berlei 46 × Ternopil 7, Zhovtolisty 36, Ukrainian sharp-leaved, Fn [(Banat × Ukrainian new) × Polish burley)] × Yellow-leaved 36, Fn Temp 321 × Sharp-leaved 451, Fn Virginia 23 × Virginia 401) with complex and individual resistance to the main diseases common in Transnistria of Ukraine. On the basis of these samples, a characteristic collection of tobacco was created and registered on resistance to biotic and abiotic factors in the National Center of Plant Genetic Resources of Ukraine.
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