Large conductance voltage- and calcium-dependent potassium channels (BK) open probability is regulated by changes in transmembrane voltage and intracellular divalent ions (Ca2+ and Mg2+) concentration. The gating ring, formed by the two RCK (Regulator of Conductance of Potassium) domains present in the C-terminal of each subunit of the tetramer, contains the divalent ion binding sites. We previously have showed that large rearrangements of the gating ring occur in a Ca2+ and voltage dependent manner. This movement of the gating ring is not directly related with the opening of the channel. It appears that a change in the voltage dependence of the open probability of the channel does not modify the voltage dependence of the movement of the gating ring. However, the voltage dependence of the movement of the gating ring is shifted toward a more negative potential if we modify the voltage dependence of the voltage sensor by co-expression of the BK alpha subunit with the Beta1 subunit or by mutations. These results indicate that the voltage dependent movement of the gating ring appears to be related to the voltage dependence of the voltage sensor and not directly related with the opening of the gate.